Microbiology Lab Work

Day 1:
Media Preparation
 For media preparation, we used 2.8g of agar in
100ml of water
 For this, we weighed the agar on a balance and then
we put it in the flask
 After that, we added water (100ml) in the flask
 The flask was autoclaved at 121°C
Sample Collection of face flora
 By using the sample sticks, we collected bacteria
from the face of our two members
 The sample sticks were then spread on the petri
dishes in a microbiology safety cabinet
 Then, these dishes were incubated overnight
 On the next day, colonies of bacteria were observed
on the plates
Day 2:
Streaking
On day 2, we performed streaking.
Procedure
 We used the T-Streak pattern for the dilution of our
bacterial culture concentration.
 First, we marked a distinct colony on the petri dish
and then picked it up by using a sterile loop.
 After that, we streaked the colony on another petri
dish containing the solidified agar.
 The whole petri dish was covered by primary,
secondary, tertiary and quaternary streaks.
 In the end, we incubated our sample dishes
overnight.
Day 3:
Preparation of Soap Solution
 In order to observe the zones of inhibition of
bacteria on our prepared petri dishes, we prepared
the different varieties of antibacterial and normal
soap solutions.
 The soaps which we used were Safeguard, Garnier
face wash, Dove, _______.
 We made a 0.1% solution by dissolving 1g of each
soap in 10 ml of distilled water in separate
graduated cylinders.
 Then the tubes were slightly shaken to dissolve the
soaps.
 After that, the different soap mixtures were
transferred to separate flasks.
 The mouths of flasks were then covered with
aluminum foils and the flasks were labelled with the
soap names.
 Then they were put in the Shaker for complete
dissolution of soap for about 24 hours.