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Sub-Topic: Effect of Amylase on Starch

Skills Assessed

C2 Making and recording observations and measurements.

C3 Interpreting and evaluating experimental observations and data.

C4 Planning, carrying out and evaluating experiments and suggesting improvements.

Aim: Investigate the effect of amylase enzyme on starch

Hypothesis: -

Apparatus and Chemicals: Dialysis tube, test tubes, water bath, Benedict’s solution,
Amylase enzyme, distilled water

Starter Questions

i. What is the role of amylase enzyme?

ii. Identify two organs in the body where amylase enzyme is produced.

iii. Briefly describe how you would test the products of this enzymatic reaction?

Safety Precautions
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Variables:

Independent Variable __________________________

Dependent Variable____________________________

Constant variable _____________________________

Design an investigation using these variables, alongside the materials provided to test
the effect of amylase enzyme on starch.

Procedure:

Result /observation: Draw a table

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Conclusion

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Explain your conclusion.

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Evaluation:

To Improve the experiment I could…………………………………………………………..

How could I make my results more reliable ………………………………………………

Questions:-

1. Describe how you would test for the presence of starch in the solutions.

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2. Why is it important to rinse the outside of the dialysis tubing bags with distilled water?

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3. The students wanted to find out if amylase enzyme passed through the dialysis tubing
into the large test tube. Amylase is made of a protein. Describe how they would test for
proteins in the large test tube.

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4. Plan an investigation to show the effect temperature on enzyme activity.

Clue:

 Identify the dependent, independent and constant variables.

 List the apparatus you would use and explain how you would measure your
dependent variable.

 Explain how you would make your results more reliable.

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