HumaLyzer 3000

| User Manual

Cat.No. 16700/1
 Revisio
Revision
n List of
of the Manua
Manual
l

No. DATE / Rev. REVISION DESCRIP RIPTION

1 03/2004-04 First
rst edition
2 04/2008-09 Ref
Reformat
3 05/
05/2008
008-11
-11 Corre
orrectctiionof small
all typi
typinger
ngerro
rorrs
1 INTRODUCT
INTRODUCTION
ION

This manual is considered as a part of the instrument; it has to be at the operator’s hand as well as at the
maintenance operator’s availability. For accurate installation, use and maintenance, please read the following
instructions carefully. In order to avoid instrument or personal damages, carefully read the ”GENERAL SAFETY
WARNINGS
WARNINGS”, ”, describing
describing the suitable
suitable operating procedures.
procedures. In case of breakdowns
breakdowns or any troubles with the
instrument, apply to the local Technical Service.

2 USER
USER WARR
WARRAN
ANTY
TY

HUMAN warrants that instruments sold by one of its authorised representatives shall be free of any defect in
material or workmanship, provided that this warranty shall apply only to defects which become apparent within
one year from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item at no charge, except for transportation
expenses to the point of repair.
This warranty excludes the HUMAN representative from liability to replace any item considered as expendable in
the course of normal usage, e.g.: lamps, valves, syringes, glassware, fuses, diskettes, tubing et c.
The HUMAN representative shall be relieved of any liability under this warranty if the product is not used in
accordance with the manufacturer's instructions, altered in any way not specified by HUMAN, not regularly
maintained, used with equipment
equipment not approved by HUMAN or used for purposes for which it was not designed.
HUMAN shall be relieved of any obligation under this warranty, unless a completed installation / warranty
registration form is received by HUMAN within 15 days o f installation of this product.
This warranty does not apply to damages incurred in shipment of goods. Any damage so incurred shall be re-ported
to the freight carrier for settlement or claim.

3 INTEND
INTENDED
ED USE OF THE INSTRU
INSTRUMEN
MENT
T [IVD]
The instrum
instrument
ent has to be used
used for the exp
expect
ected
ed purpose
purposess and
and in perfec
perfectt techn
technica
icall condit
condition
ions,
s, by qualif
qualified
ied
personnel, in working conditions and maintenance operations as described in this manual, according to the
GENERAL SAFETY WARNINGS. This manual contains instructions for pr ofessional qualified operators.

4 GENER
GENERAL
AL SAFE
SAFETY
TY WARN
WARNIN
INGS
GS

Use only chemical reagents and accessories specified and supplied by HUMAN and/or mentioned in this manual.
Place the product so that it has proper ventilation.
The instrument should be installed on a stationary flat working surface, free from vibrations.
Do not operate in area with excessive dust.
Work at room temperature and humidity, according to the specifications listed in this manual.
Do not operate this instrument with covers and p anels removed.
Only use the power cord specified for this product, with the grounding conductor of the power cord connected to
earth ground.
Use only the fuse type and rating
rating specified by the manufacturer
manufacturer for this instrument
instrument,, use of fuses
fuses with improper
ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the instrument.
Do not power the instrument in potentially explosive environment or at r isk of fire.
Prior to cleaning and/or maintaining the instrument, switch off the instrument and remove the power cord.
For cleaning use only materials specified in this manual, otherwise parts may become damaged.
It is recommended always to wear protective apparel and eye protection while using this instrument.
Respective warning symbols, if appearing in t his manual, should be carefully considered.
co nsidered.
5 DISPOSAL MANAGEMENT CONCEPT

The currently valid local regulations governing disposal must be observed. It is in the responsibility of the user to
arrange proper disposal of the individual components.
All parts which may comprise potentially infectious materials have to be disinfected by suitable validated
procedures (autoclaving, chemical treatment) prior to disposal. Applicable local regulations for disposal have to be
carefully observed.
The Instruments and electronic accessories (without batteries, power packs etc.) must be disposed of according to
the regulations for the disposal of electronic components.
Batteries, power packs and similar power source have to be dismounted from electric/electronic parts and disposed
off in accordance with applicable local regulations.

6 INSTRUMENT DISINFECTION

Analytical instruments for in vitro diagnostic involve the handling of human samples and controls which should be
considered at least potentially infectious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as potentially infectious.
Before doing any servicing on the instrument it is very important to thoroughly disinfect all possibly contaminated
parts. Before the instrument is removed from the laboratory for disposal or servicing, it must be
decontaminated/disinfected. Decontamination/disinfection should be performed by a authorised well-trained
personnel, observing all necessary safety precautions. Instruments to be returned have to be accompanied by a
disinfection certificate completed by the responsible laboratory manager. If a disinfection certificate is not
supplied, the returning laboratory will be responsible for charges resulting from non-acceptance of the instrument
by the servicing centre, or from authority’s interventions.

7 NOTICE

Every effort has been made to avoid errors in text and diagrams, however, HUMAN GmbH assumes no
responsibility for any errors which may appear in this publication. It is the policy of HUMAN GmbH to improve
products as new techniques and components become available. HUMAN GmbH therefore has to reserve the right
to change specifications if necessary in the course of such improvements.
NOTICE
Analytical instruments for in vitro diagnostic application involve the handling of human samples and controls
which should be considered at least potentially infectious. Therefore every part and accessory of the respective
instrument which may have come into contact with such samples must equally be considered as potentially
infectious.

BIOHAZARD

The „BIOHAZARD“ warning label must be affixed to instrument prior to first use with biological material !

Servicing Note:
Before doing any servicing on the instrument it is very important to thoroughly disinfect all possibly contaminated
parts. Before the instrument is removed from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination should be performed by authorised well-trained personnel only, observing all necessary safety
precautions. Instruments to be returned have to be accompanied by a decontamination certificate completed by
the responsible laboratory manager. If a decontamination certificate is not supplied, the returning laboratory will
be responsible for charges resulting from non-acceptance of the instrument by the servicing centre, or from
authority’s interventions.

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
| Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
| Tel.: +49 61 22/99 88-0 · Fax: +49 61 22/99 88-100
| e-Mail: tech-support@human.de · www.human.de
 Contents

1 INTRODUCTION 5
1.1 Applications 5
1.1.1 Intended Use 5
1.1.2 Summary of the Instrument 5
1.2 Specifications 5
1.3 Warning Markings 7
1.3.1 Safety Symbols 7
1.3.2 Safety Terms 7
1.4 Safety Precautions 7
1.5 Setup 9
1.5.1 Unpacking 9
1.5.2 Installation/Preparation 9
1.5.3 Keypad Description 11
1.6 Checkout 11
1.7 Getting Started 12
1.7.1 Set Date/Time and Laboratory Name 12
1.7.2 Printer Set Up 13
2 OPERATING PROCEDURES 14
2.1 General Selections 14
2.1.1 Flowcell Configuration 14
2.1.2 Lamp Warm-up and Lamp Saver 15
2.1.3 External Ports 15
2.1.4 Serial Port 15
2.1.5 PC Connection 16
2.1.6 Error Messages While Using the Parallel or Serial Ports 16
2.1.7 Units of Measurement 16
2.1.8 Entering Names 17
2.1.9 Ranges and Controls 17
2.1.10 Reports 20
2.1.11 Blanking 21
2.1.12 Reading Samples 21
2.1.13 Bichromatic Operation (Differential Filter) 22
2.2 Calculation Programs 22
2.2.1 Absorbance Mode 22
2.2.2 Single Standard Mode 23
2.2.3 Factor Mode 25
2.2.4 Multi-point Mode 25
2.2.5 Rate Mode 27
2.3 Stored Tests 31
2.3.1 Recalling a stored test 32
2.3.2 Listing stored tests 32
2.3.3 Deleting a test 32
2.3.4 Editing a test 32
2.3.5 Using WORKLIST 33
2.4 Special Features 33
2.4.1 Self-Check 33
2.1.8 Entering Names
It is sometimes necessary to enter an alphanumeric name. For instance, user tests and controls can be named, and
the laboratory name shown in the header may be changed. When prompted for a name, the following display is
shown if no external keyboard is connected:

The cursor is the small triangle beneath the “A” on the top line. Press 4 to move the cursor to the left, 6 to move the
cursor to the right, 2 for up, or 8 for down. When the cursor is beneath the first letter of the chosen name, press
SELECT. The bottom line clears and shows the selected letter. Continue using the 2, 8, 4 and 6 keys and SELECT to
select each letter in the name. When complete, press COMPLETE. To remove a letter from the end of the name,
press F2 (Back Sp.). To cancel and return to the main prompt, press QUIT.

The HumaLyzer 3000 offers the option of using an External Keyboard. If it is plugged in before turning instrument
On, the External Keyboard is automatically activated by pressing any key. If the External Keyboard is active, each
time the instrument prompts the user to “Enter Name”, the External Keyboard alone will be used and the KeyPad
screen (shown in this section) will not appear. The External Keyboard’s key usage limitations are listed as follows:
 Any letter or number key are available for data entry.
 The F1 - F4 keys work the same way that the F1 thru F4 keys work on the keypad.
 The Shift or CAPS LOCK keys can be used to change the case of the characters.
 Windows Specific keys have no function. (i.e. Ctrl, Alt, Tab, Esc, etc.)

2.1.9 Ranges and Controls

In all modes except Absorbance Mode, the user has the option of entering ranges and / or controls. The display will
show:
Press “1” (Set Up Ranges). The display shows:

If applicable, enter the Blank Abs. Min and Max values. In this example, those values were skipped (Any value can
be skipped by simply pressing ENTER). Enter the concentration limits for the Normal Range and Linear Range.
The display will show:

The user may also change any of the values by using the CLEAR (F3) key or the ARROW (F2) Key. When the
instrument takes a reading, a word indicating the range (none, LOW, HIGH, OUT) is shown to the right of the
concentration on both the display and the printer.
None: The concentration is within the normal range.
LOW: The concentration is lower than the Minimum value of the Normal range, but within the linear range.
HIGH: The concentration is higher than the Maximum value of the Normal range, but within the linear range.
OUT: The concentration is outside the Linear range.
If finished, press ENTER when COMPLETE is highlighted.
The user is returned to the Results Parameters Setup Menu. Press 2 to Setup controls. The instrument allows the
user to enter and name up to 3 controls per test. Controls are designated samples with specified concentration
ranges that provide a reference for comparisons. The display shows:
Select 1, 2 or 3 and Enter to choose the desired control type. A Normal control is used in this example. Select 1. The
display shows:

From this menu, if F1 (QUIT) is chosen, the instrument returns to the Main Menu and quits the test. Press F4
(Disable) and return to the Result Parameters Setup Menu. If F3 (ENABLE) is selected, the instrument prompts to
“Enter Normal Control Name”. (For more information, see section 2.1.8 Entering Names.) Once completed, the user
is prompted to “Enter Normal Control Lot Number”. Enter this number in the same way.
Once complete, the instrument prompts to “Enter Normal Control Expiration Date:” Using the keypad or key-board,
enter the expiration date in the MM.YY format. For instance, if the expiration date is “September 2007” the user
will enter “09.07”. When finished, press F4 (ENTER). The display will prompt you to enter a Low Range Limit.

Enter the Low Range and press Enter. Then input the High Range and press Enter. The display now shows:

The user should understand that in all selections except #4, passing control data is saved. Select the number which
corresponds with the reaction that should be taken in the event of a failed control and press Enter. The display will
return to the Control Selection menu. Select another control or press 4 to return to the Result Parameters Setup
Menu.
Control results can be saved and used later to generate a Levey-Jennings report. (See section 2.1.10 Reports for
more info.)
When finished, select 3, Setup Complete.
Then gives the test number, then the name the user saved it as. If it is the first saved test and is named HIV, the
display and printout will show:
Saving Test # 1 HIV

If in tube mode, the instrument now makes an air reference reading. If Flowcell is active, it uses the stored reading.
The display then shows:

Insert the blank tube or sample the blank material. See the section “ 2.1.11 Blanking” for details. When finished
reading, remove the tube or sample the material. Re-blanking may be done at any point by pressing BLANK.

The display shows:

Remove the sample and repeat as necessary. To exit Absorbance Mode and return to the main prompt, press QUIT
(F1). The instrument prints “Test Ended” and returns to the main prompt.

2.2.2 Single Standard Mode

In Single Standard Mode, the instrument reads and prints absorbances, and calculates concentrations based on a
standard material of known concentration. Results are calculated according to Beer’s Law. The calibration factor is
printed for future use.

Press PROGRAM (F2). Select 2. The display shows:

Standard Mode
Differential Samples?

Press YES to use differential samples. This works exactly as described below, except that each sample has its own
blank, rather than using the same blank for all subsequent samples. The instrument automatically prompts for the
blank preceding each sample. If not using differential samples, press NO to continue.
The Select Filter Screen will be shown. Primary Filter is Highlighted
Press the numeric key that corresponds to the desired wavelength. To confirm the choice, press ENTER. The selected
wavelength is highlighted.
Differential Filter is then Highlighted. Select the differential wavelength in the same way the primary wavelength
was chosen and press ENTER. The Wavelengths are then printed.
The display shows:

Enter the value of the standard and press ENTER. To clear a mistake and re-enter the factor press CLEAR. The
standard concentration is then printed.
Note: The instrument will accept a factor which is up to seven digits, and there can be up to (2) decimal places.
The instrument then prompts for a unit of measure to be entered. Enter the unit’s code (0-13) and press ENTER. See
the section “2.1.7 Units of Measurement” for details. The entire list of Units is displayed. Type in the corresponding
number and press ENTER. The display then shows the selected unit. Press YES to accept.
The Result Parameters screen is then shown prompting the user to set up Ranges and Controls. See section 2.1.9
Ranges and Controls for more on this option.
The next two options are first to save the test and then to name the test. If yes to saving the test, the option will
then be given to name the test. When a test is saved, it will always be saved as the next available number. 1, 2, 3, 4,
etc. After naming the test and pressing COMPLETE, Display shows:
Saving Test #

Then gives the test number, then the name the user saved it as. If it is the first saved test and is named HIV, the
display and printout will show:
Saving Test # 1 HIV

If the instrument is in tube mode, it displays “Referencing Air” and makes an air reference reading. In Flowcell
mode, the stored water values are used as a reference.
Insert the blank tube or sample the blank material. See the sections “2.1.14 Blanking” and “2.1.15 Reading
Samples” for details.
The display shows:

Insert the standard tube or sample the standard material. See the section “2.1.15 Reading Samples” for details. The
instrument will read the absorbance and determine the factor such that the concentration of the standard is the
value that was specified. The Standard Reading calculated factor will be printed. Repeat as necessary.
Re-Blanking may be done at any point by pressing BLANK. To exit Standard Mode and return to the main prompt,
press QUIT. The printer outputs “Test Ended”, and the instrument returns to the main prompt.
2.2.3 Factor Mode
In Factor Mode, the instrument reads absorbances at the selected wavelengths, and calculates concentrations by
multiplying the absorbance by the user supplied factor.
Press PROGRAM (F2). Select 3. The display shows:
Factor Mode
Differential Samples?

Press YES to use differential samples. This works exactly as described below, except that each sample has its own
blank, rather than using the same blank for all subsequent samples. The instrument automatically prompts for the
blank preceding each sample. If not using differential samples, press NO to continue.
The Select Filter Screen will be shown. Primary Filter is highlighted. Press the numeric key that corresponds to the
desired wavelength. To confirm the choice, press ENTER. The selected wavelength is highlighted.
Differential Filter is then Highlighted. Select the differential wavelength in the same way the primary wavelength
was chosen and press ENTER. The Wavelengths are then printed.
Enter the Factor when prompted and press ENTER. To clear a mistake and re-enter the factor press CLEAR. When
ENTER is pressed, the factor is shown on the printer.

Note: The instrument will not accept a factor which is more than seven digits, and there can be up to (2) decimal
places.

Enter the unit’s code (0-13) and press ENTER. See the section “Units of Measurement” for details. The entire list of
Units is displayed. Type in the corresponding number and press ENTER. The display then shows the selected unit.
Press YES to accept.
The Result Parameters screen is then shown prompting the user to set up Ranges and Controls. See section 2.1.9
Ranges and Controls for more on this option.
The next two options are first to save the test and then to name the test. If yes to saving the test, the option will
then be given to name the test. When a test is saved, it will always be saved as the next available number. 1, 2, 3, 4,
etc. After naming the test and pressing COMPLETE, display shows:
Saving Test #

Then gives the test number, then the name the user saved it as. If it is the first saved test and is named Test HIV,
the display and printout will show:
Saving Test # 1 TEST HIV

Read the sample Blank. Then continue with normal sampling following the prompts given by the instrument. See
the sections “2.1.14 Blanking” and “2.1.15 Reading Samples” for details.
To exit the mode and return to the main prompt, press QUIT. The printer outputs “Test Ended”, and the instrument
returns to the main prompt.

2.2.4 Multi-point Mode

In Multi-point mode, the instrument reads and prints absorbances, and calculates the concentration based on the
concentrations of the standards. Up to seven standards can be entered. The absorbances of the standard are used
to construct a point-to-point curve which passes through all of the standards and the point (0,0). Unknown
samples are calculated as follows: First, the unknown sample’s absorbance is calculated and compared to the
standard absorbances. The line segment of the standard curve used to determine the concentration of the un-
known is the line connecting the pair of standards whose absorbances are closest above and below the unknown
absorbance.
An unknown sample with absorbance greater than the highest standard absorbance is calculated on the line
passing through the highest 2 standard points. An unknown sample with absorbance less than the lowest standard
absorbance is calculated on the line passing through the lowest 2 standard points.
For samples requiring flowcell aspiration volumes of 350 µl or less, the shorter sample tube (provided in the tubing
kit) must be installed. Refer to the section “Flowcell tubing replacement”.
Press F2 (PROGRAM) then 4. The display shows two choices. Press 1 and Enter to choose Standard Multi-Point
Mode, 2 and Enter for Multi-Point % Absorbance Mode. The Multi-point % Abs mode is similar in calculation to the
Multi-Point mode, except that the percent absorbance is calculated and printed, and the standards must be in
descending order.
3 CLEANING AND MAINTENANCE
3.1 Cleaning
3.1.1 Exterior
CAUTION: Solvents such as acetone or thinner will damage the instrument! Use only water and recommended
cleaners! Avoid abrasive cleaners. The keypad and display areas are liquid-resistant, but are easily scratched.

The exterior of the instrument may be cleaned with a soft cloth using plain water. If needed, a mild all-purpose
(nonabrasive) cleaner may be used. A 1.5% solution of chlorine bleach or 70% isopropyl alcohol may be used as a
disinfectant. Take special care not to spill any liquid into the read well.

3.1.2 Flowcell
The Flowcell should be cleaned when the instrument will not be used for an extended period, e.g. overnight, end of
shift, and when storing the Flowcell. Proper cleaning will help to prevent clogging of the Flowcell tubing and valve
tubing. Cleaning is extremely important to obtaining accurate, repeatable results. If reagent, serum, or other
proteinaceous fluid is allowed to dry in the Flowcell, it is extremely difficult to remove and its presence can affect
test results.
To clean the Flowcell:
1. Purge with air for at least 5 seconds.
2. Aspirate a small amount of FLOW CELL CLEANER ( [REF] 18222). Allow the solution to remain in the Flowcell for
3 minutes.
3. Aspirate 15 ml of distilled water then purge with air for 5 seconds.
4. Aspirate 0.1N hydrochloric acid (HCl). Allow the solution to remain in the Flowcell for 3 minutes.
5. Purge with at least 15 ml of deionised water.
6. Leave the Flowcell filled with water.
7. If preparing the Flowcell for storage, follow same instructions but purge completely after cleaning.

Material required

[REF] 18222 FLOW CELL CLEANER 100 ml

Detergent 0.1%
NaOH < 0.5%
Sodium azide 0.1%

Chemicals
HCl 0.1 N

Disinfectant:
Isopropylalcohol 70% or
Sodiumhypochlorite (bleach) 1.5%

Warning
The above chemicals present the following hazards and should be handled with due care:
HCl and sodium hypochlorite are corrosive and toxic solutions which cause severe burns.
Propane-2-ol (isopropyl alcohol) is highly inflammable and harmful.

3.1.3 Waste Bottle

The waste bottle may be autoclaved or it may be cleaned with a commercially available all-purpose cleaner or
disinfectant. A 1.5% chlorine bleach solution or 70% isopropyl alcohol may also be used. Always turn instrument
“OFF” before disconnecting the waste bottle.
3.2 Maintenance
3.2.1 Calibration and Linearity
Each instrument is calibrated during manufacturing using standards that are traceable to the National Institute for
Standards and Testing (NIST), and is tested to verify its linearity to 2A. This pre-set calibration is very stable.
Absolute calibration can be verified with the use of NIST filters, or by periodic comparison to a reference instru-
ment that is known to be calibrated to NIST filters. Calibration may be confirmed using Awareness Technology’s
Redi-Check, a commercially available calibration check set which can be obtained from your distributor. A periodic
verification of instrument linearity is advised.
Since most lab test results are based upon standards rather than upon absolute absorbance, the linearity of the
instrument is the more critical indicator of instrument performance. A reduction in linearity with age may be in-
dicative of optical filter deterioration. In this event, filter replacement is required for continued reliable operation.
The best way to assure quality instrument performance is to include a sufficient number of controls in each as-say
to cover the entire operational range.

3.2.2 Opening the Instrument

Refer to Figure 2 - Instrument Interior. The cover is hinged at the rear panel, and can be raised to allow access to the
inside of the instrument. Disconnect the power cable, the tubing, and the sensor lead from the rear panel. Remove
the waste bottle. Move the instrument forward until the front edge overhangs the work surface. Locate and
remove the cover screw from the middle of the underside of the front lip. Gently lift the front of the cover upward,
taking care to clear the photometer. Prop the cover open with a suitable object.
Do not force the cover backwards. Damage to the cover or fittings may result.
To reinstall the cover, reverse the procedure. Carefully lower the cover until it seats on the chassis, taking care to
clear the incubation block and the Flowcell Luer fitting.

MAIN PCB
Power
Supply

Pump

Photometer

Fan

Valve

Remove screw from

underneath to remove cover

Figure 2
3.2.3 Lamp Replacement
The lamp should be replaced only if it fails to light, or several filter voltages are reported as low.
To replace the lamp, follow the procedure below. Turn Power “Off” and unplug instrument.
Open the instrument as described in “Opening the Instrument”. Locate the photometer, and the lamp at the right
side of the photometer. Refer to Figure 3, Lamp Removal and Replacement. The figure shows the right side view of
the photometer assembly.
CAUTION: Lamp is HOT. Allow the lamp to cool before handling.
Loosen but do not remove the 2 centre lamp connector screws. Remove the lamp by lifting upward.
Use a pair of pliers or tweezers to handle the new lamp. Avoid handling with bare skin. Insert the lamp leads into
the connector until they hit bottom. Refer to Figure 3, Lamp Alignment. The lamp filament must be centred on the
lens and the lamp body must be parallel with the lens bracket. While holding the lamp in alignment, tighten the
lamp connector screws.
Set the power switch to ON. Observe the projection of the light from the lamp onto the cell holder (behind the
lens). Refer to Figure 3, Spot Alignment. The spot should be small and centred on the oval hole in the cell block
(behind the lens). If the spot is not centred, use the adjustment screws to position the spot. The vertical adjustment
screw raises and lowers the lamp bracket. The lamp bracket is slotted at the horizontal adjustment screw, so that
the lamp bracket can be moved. The horizontal adjustment screw serves to lock down the lamp bracket.
Insert a borosilicate 12 mm tube filled with plain water into the read well. Do not use a soda-lime glass tube, since
it does not transmit at 340nm. Press F4 (TOOLS). Press 4 and ENTER. Press 1 and ENTER The display shows the
voltages for each filter.

Figure 3
Follow these steps to restore the electronic calibration:
1. Shut off the instrument. Remove any tubes or cuvettes from the read well. Carefully lift up the instrument and
locate the Calibration Data label on the underside of the unit. There are (2) values recorded there: Absorbance
and Cell Temp. Write down these numbers.
2. Set the power switch to ON(1).
3. If the date and time have been reset or are incorrect, enter the correct date and time. See the section “Set Date
and Time”
4. Press TOOLS (F4). Press 4 and Enter. The Diagnostics Menu is shown. Choose 3 for Absorbance Calibration and
Enter. Enter the Number.
5. Choose 4 for Temp Calibration and Enter. Enter the Number.
6. Press Run Test (F1), type in 213, and press ENTER to get a report of the calibration data. The cell temperature
adjustment will be printed along with the absorbance adjustment. Make sure that these values are the same as
those recorded on the calibration label.

Restore Filter Labels

Like the calibration data, the wavelengths for the two optional filters are stored in non-volatile memory. In the
event this data is lost or corrupted, the following message will be displayed and printed.
Filter Labels 7&8 Clrd!

Filter labels need to be re-entered for two of the filters. Open the instrument and locate the filter label on the side
of the photometer cover.
Key 7 is xxx
Key 8 is xxx

where “xxx” is a three-digit wavelength value. If there are no 7th or 8th filters, they will be listed as BLOCKED. Press
RUN TEST (F1), type in 248, and press ENTER. The display prompts:
Key 7 = ??? nm

Type in the wavelength for Key 7 that is printed on the label and press ENTER. Repeat for Key 8. use “000” for
unused filter positions. Press QUIT to return to the main prompt. Note that, if values for Key 7 and Key 8 are
entered when there are no filters present, the filters will be flagged as “low” when Self Check is run.
For Key 8. use “000” for unused filter positions. Press QUIT to return to the main prompt. Note that, if values for Key
7 and Key 8 are entered when there are no filters present, the filters will be flagged as “low” when Self Check is run.

Other Tests:
Vacuum count
The last 4 readings are averaged together and compared to 200
Greater than 203 shuts the vacuum off
Less than 197 turns it on.

Cell and block temp use the same cut-off numbers.

The last 4 readings are averaged together and compared to 462
Greater than 466 turns the heat off.
Less than 458 turns the heat on.

The Block heat temperature is not displayed if the count is less than 250

Waste count
A count of less than 150 is considered a full bottle condition.

The "Filter Wheel Speed" menu selection provides the user with the current wheel speed. The speed is set to
300 +/- 10
5 CONTACT INFORMATION
In the unlikely event that a problem is experienced with the instrument, please consult your dealer first.
Dealer:

If the dealer is unable to resolve the problem, our support staff at Human GmbH is happy to assist, and can be
reached in Germany by:

Fax: (49) 6122 – 9988 - 100

E-mail: tech-support@human.de
Mail: Human GmbH
Max-Planck-Ring 21
D-65205 Wiesbaden
Germany

When contacting Human GmbH, please provide the following:

The serial number of the instrument
A description of the problem with as much detail as possible
Printouts, which can be submitted by mail, fax or e-mail
HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
| Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
| Tel.: +49 61 22/99 88-0 · Fax: +49 61 22/99 88-100
| e-Mail: human@human.de · www.human.de