Professional Documents
Culture Documents
The good old lager is gaining in popularity around the world among
craft brewers, but with nowhere to hide this is not the easiest style
to master.
Krishna Rajiv & Simon Jeanpierre (Fermentis) will be sharing a
quick trouble shooting guide along with remedies for some of the
issues they have seen which puts many breweries off brewing a
lager; notably fermentation temperature, neutrality, diacetyl
andacetaldehyde.
By the end of the session you will be able to brew a good lager
with confidence thanks to the experts tips and tricks – even in the
most adverse of conditions found around the region.
This session’s key Take-Away’s. Learn to:
- Avoiding off flavours
- Producing a crisp, clean lager beer
- Enhancing the raw material characteristics in your lager
SEABrew Sessions
The Lost art of Lagers: Linking new drinkers to
Craft Lagers
Simon Jeanpierre and Krishna Rajiv, Fermentis © Fermentis communication – All rights reserved
Agenda
Often lager beer was stored and fermented in cool caves and cellars prior to artificial refrigeration
arriving in the late 1800s. Refrigeration made reliable lager beer brewing possible year-round,
Helles was an early arrival
Aging: chemical reactions take place slowly during cold storage, and the flavour D.G. Yuengling & Son brewery caves, Pennsylvania, USA 1840
profile of the beer further develops. Cell autolysis responsible for some of the
changes in lagering (cell releases material into the beer, e.g. amino acids, peptides,
nucleotides, phosphates)
Q: Is long, cold lagering still absolutely necessary…?
MOUTHFEEL
CLARITY …”higher temperature.. faster
… “a short period (6 hours) release of fatty acids into
FLAVOUR at a very low temperature beer…. The rate at 20°C being Modern methods
… ”once Vicinal diketones and (e.g., −2.5°C) is more 20 times that in 0°C in
acetaldehyde have been dealt efficacious than a horizontal tanks… fatty acids versus traditional
with, there is no merit in the prolonged period at a are needed in certain amounts techniques…
prolonged storage of beer”.. warmer temperature (e.g., to give the proper mouthfeel”
(Metrulas et al, J. Inst. Brew. 0°C)” (Brewing New Technologies:
2019; 125: 242-249) (Miedl & Bamforth, J. Am. Chapter 12, ‘Accelerated
Soc. of Brew. Chem. Vol. Processing of Beer’, Bamforth
62, 2004 - Iss. 2) 2006) © Fermentis communication – All rights reserved
5
Differences between lager and ale yeasts
Lager
Lower
Lower
Flavour diversity?
The Baseline Study
• Pitching : 50g/hl
• Temperature : 23°C
• Atmospheric pressure
Analytical Sensory
Lagers: A
similar ester
profile at
23°C
Saccharomyces
pastorianus
°P
Yeast °C
Strain
Pitching
Rate
Fermentation Performance
Volatiles Profile
Sensory Analysis
°P 1 6 0P PROTOCOL
2 0 0P • All malt wort (pils)
• 28 EBU
1 2 0C • Direct pitching
1 6 0C
°C 2 0 0C STUDIED CONDITIONS (14)
2 4 0C
Temperature Pitching rate
BE256 Scale Condition Density (°P)
(°C) (g/hL)
25g/hL 1 Pilot C1 16 12 50
Pitching 50g/hL 2 Pilot C2 20 24 50
Rate 3 Pilot C3 20 24 100
100g/hL 4 Pilot C4 12 20 50
200g/hL
5 Pilot C5 16 20 50
6 Pilot C6 20 20 50
7 Pilot C7 16 20 25
8 Pilot C8 16 20 100
9 Pilot C9 12 20 100
10 Pilot C10 16 16 100
11 Pilot C11 12 12 25
Fermentation Performance 12 Pilot C12 12 16 50
time (h) 13 Pilot C13 20 16 25
14 Pilot C14 20 12 100
Volatiles
LAB TRIALS 100ML
C1-P 20
C2-P
✓ 4 to 19 days of fermentation
Extract (°P)
1.5
0.5
0
C1-P C2-P C3-P C4-P C5-P C6-P C7-P C8-P C9-P C10-P C11-P C12-P C13-P C14-P
Neutral across all
n-propanol Isobutanol (isobutyl alcohol) Amyl alcohol Isoamyl alcohol Phenylethyl alcohol fermentations!!
1.5
0.5
0
C1-P C2-P C3-P C4-P C5-P C6-P C7-P C8-P C9-P C10-P C11-P C12-P C13-P C14-P
* ^
^
LS means
C11-P = off-note
Temperature (°C)
Isoamyl acetate 0,859 -0,541 15
Ethyl butyrate 0,994 -0,758
Ethyl hexanoate 0,838 -0,643
10
Ethyl octanoate 0,600 -0,388
Hop Components
°P
Analysis
Monoterpene
Alcohols
Hop derived Esters Terpenes °C
Ox. Sesquiterpenes Ketons Aldehydes
Linalol 2+3 Methylbutylpropanoate β-Myrceen Caryophylleen oxide β-Damascenon Citral
3-Methylbutyl-2-
Geraniol α-Humuleen 2-Undecanon
methylpropanoaat Pitching
2-Methylbutyl-2- Rate
Citronellol Limonene
methylpropanoaat
2-Methylbutyl-3-
α-Terpineol
methylbutanoate
1-Terpinen-4-ol Methyloctanoate
Methylgeranate
Geranylacetaat
Late Hopping - Added at beginning of pumping the wort in the whirlpool Fermentation Performance
(pumping takes 10 minutes, then 15 minutes of settling), around 90°C
Volatiles Profile
Dry Hopping* - added 24h after filling of vessel, Fermentation day 1 (24
°C). Sensory Analysis © Fermentis communication – All rights reserved
The case of W-34/70
1.5
T58_Dh
Herbal BE134_Lh
1
Gen. Hoppy
S189_Lh
US05_Dh
S23_Dh
T58_Lh S04_Dh
S23_LDh Floral K97_Dh
0.5 S33_Dh
WB06_Lh
S04_LDh
Bitterness BE134_Dh
W3470_Dh Afterbitterness BE134_LDh
0
F2 (27,07 %)
US05_LDh S189_Dh
WB06_LDh
W3470_Lh S33_LDh
Fruity
W3470_LDh S23_Lh K97__LDh
-0.5
S189_LDh
S04_Lh T58_LDh
-1
WB06_Dh
K97_Lh
US05_Lh
-1.5
S33_Lh
-2
-2 -1.5 -1 -0.5 0 0.5 1 1.5 2
F1 (36,26 %)
© Fermentis communication – All rights reserved
The gypsy lager strain S-189
1.5
BE256_LDh
T58_Dh
Herbal BE134_Lh
1
Gen. Hoppy
S189_Lh US05_Dh
S23_Dh
T58_Lh S04_Dh
S23_LDh Floral
0.5
BE256_Dh
WB06_Lh
S04_LDh
Bitterness BE134_Dh
W3470_Dh BE134_LDh
Afterbitterness
0
S189_Dh
F2 (27,07 %)
US05_LDh
WB06_LDh
W3470_Lh
Fruity
W3470_LDh S23_Lh
BE256_Lh
-0.5
S189_LDh
S04_Lh T58_LDh
-1
WB06_Dh
US05_Lh
-1.5
-2
-2 -1.5 -1 -0.5 0 0.5 1 1.5 2
F1 (36,26 %) © Fermentis communication – All rights reserved
S-23 … 1.5
BE256_LDh
T58_Dh
Herbal BE134_Lh
1
Gen. Hoppy
S189_Lh
US05_Dh
S23_Dh
T58_Lh S04_Dh
0.5
S23_LDh Floral
BE256_Dh
WB06_Lh
S04_LDh
BE134_Dh
Bitterness
W3470_Dh BE134_LDh
Afterbitterness
0
F2 (27,07 %)
US05_LDh S189_Dh
WB06_LDh
W3470_Lh
Fruity
W3470_LDh S23_Lh
BE256_Lh
-0.5
S189_LDh
S04_Lh T58_LDh
-1
WB06_Dh
US05_Lh
-1.5
-2
-2 -1.5 -1 -0.5 0 0.5 1 1.5 2
F1 (36,26 %)
© Fermentis communication – All rights reserved
Yeast and Cascade
Conclusion: Part 1
❖But…
© Fermentis communication – All rights reserved
❑Integrating a ‘Blanco’ (non
hopped)
B L A N C O + 1 L AT E H O P P I N G R E G I M E S
NO LATE Hopping 0
Late Hopping + Dry Hopping* 200+200
Late Hopping - Added at beginning of pumping the wort in
the whirlpool (pumping takes 10 minutes, then 15 minutes
of settling), around 90°C
Dry Hopping* - added 24h after filling of vessel,
Fermentation day 1 (24 °C).
➢ + Detailed Sensory
(discriminating
fermentation from
hop flavours)
➢ + Analytics
© Fermentis communication – All rights reserved
Yeast & Cascade study with blank/non-hopped control
2 2
Gen. Hoppy
Phenolic, clovy Phenolic, clovy Gen. Hoppy Expression
Expression
0 0
Lagers
S189 S23 W3470
8
7
Sensory LS means
6
5
4
3
2
1
0
Turbidity Malt Fruity Global Gen. Hoppy Hop Herbal, Floral HOP Citric HOP Fruity Fruity Apple Phenolic, Sulfury Bitterness
level Character Expression Vegetal Tropical BANANA clovy
6
5
4
3
2
1
0
Turbidity Malt Fruity Global Gen. Hoppy Hop Herbal, Floral HOP Citric HOP Fruity Fruity Apple Phenolic, Sulfury Bitterness
level Character Expression Vegetal Tropical BANANA clovy
SUGAR PROFILE
60 65 70
Disaccharide 61 55 41
Trisaccharide 9 12 16
Monosaccharide 10 9 8
Dextrins 20 24 35
13.5°P 18°P
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