SM - V4.1

Photometer 5010
Version 4.x
upwards serial no. 5000
Service Manual
CONTENTS
chapter headline page file
1 General notes Sm_V4_a.pdf

1.1 Contact partner 1.1 Sm_V4_a.pdf
1.2 Proved security 1.1 Sm_V4_a.pdf
1.3 Precautions and hazards 1.2 Sm_V4_a.pdf
2 Service concepts Sm_V4_a.pdf

2.1 Service level 2.1 Sm_V4_a.pdf
2.2 Conditions 2.2 Sm_V4_a.pdf
3.1 Block diagram 3.1.1 Sm_V4_a.pdf

3.2 Short instruction guide 3.2.1 Sm_V4_a.pdf
3.3 Service tools 3.3.1 Sm_V4_a.pdf
3.4 Plug identification 3.4.1 Sm_V4_a.pdf
4.1 Mains power circuit 4.1 Sm_V4_b.pdf

4.2 Mains connector 4.2 Sm_V4_b.pdf
4.3 AC / DC power supply 4.3 Sm_V4_b.pdf
4.10 Optical unit 4.10.1 Sm_V4_b.pdf

4.11 Filter wheel 4.11.1 Sm_V4_b.pdf
4.12 Halogen lamp 4.12.1 Sm_V4_b.pdf
4.13 Photodiode with plug 4.13.1 Sm_V4_b.pdf
4.14 Heating unit 4.14.1 Sm_V4_b.pdf
4.15 Peltier element 4.15.1 Sm_V4_b.pdf
4.16 Pump motor 4.16.1 Sm_V4_b.pdf
4.17 Tooth belt 4.17.1 Sm_V4_b.pdf
4.18 Step motor filter wheel 4.18.1 Sm_V4_b.pdf
4.19 Light barrier filter wheel 4.19.1 Sm_V4_b.pdf
4.20 Bubble detector 4.20.1 Sm_V4_b.pdf

4.21 Flow-through cuvette adaptor 4.21.1 Sm_V4_b.pdf
4.22 Standard cuvette adaptor 4.22.1 Sm_V4_b.pdf
4.23 Connector bubble detector 4.23.1 Sm_V4_b.pdf
4.24 Lamp holder 4.24.1 Sm_V4_b.pdf
4.25 Cuvette lense kit 4.25.1 Sm_V4_b.pdf
4.26 Tubes 4.26.1 Sm_V4_c.pdf
RIELE BERLIN Photometer 5010 / V4 1 22.09.2003

CONTENTS
4.30 Top case 4.30.1 Sm_V4_c.pdf

4.31 Display glass 4.31.1 Sm_V4_c.pdf
4.32 Metal tube inlet 4.32.1 Sm_V4_c.pdf
4.33 Micro switch 4.33.1 Sm_V4_c.pdf
4.34 Keyboard 4.34.1 Sm_V4_c.pdf
4.35 Printer mechanism 4.35.1 Sm_V4_c.pdf
4.40 Mother board RR_115 Sm_V4_d.pdf

General description 4.40.1 Sm_V4_d.pdf
Component diagram 4.40.2 Sm_V4_d.pdf
Voltages 4.40.3 Sm_V4_d.pdf
Voltage 12 VDC of halogen lamp 4.40.4 Sm_V4_d.pdf
Fuses F1, F2 and F3 4.40.4 Sm_V4_d.pdf
Amplifier of photocurrent and ADC 4.40.5 Sm_V4_d.pdf
Adjustment of amplifier stage 4.40.6 Sm_V4_d.pdf
Contrast of LCD 4.40.7 Sm_V4_d.pdf
Temperature control 4.40.8 Sm_V4_d.pdf
Adjustment of temperature control 4.40.9 Sm_V4_d.pdf
Thermal adjustment 4.40.10 Sm_V4_d.pdf
Installation instruction 4.40.11 Sm_V4_d.pdf
Circuit diagrams 4.40.13 Sm_V4_e.pdf
Circuit diagrams 4.40.17 Sm_V4_f.pdf
4.42 Display unit pcb RR-114 4.42.1 Sm_V4_g.pdf

4.43 Cuvette connection pcb RR-103-V4 4.43.1 Sm_V4_g.pdf
4.44 Printer controller pcb 4.44.1 Sm_V4_g.pdf
4.50 Fan 4.50.1 Sm_V4_g.pdf

4.51 Bottom case 4.51.1 Sm_V4_g.pdf
4.52 Carton Photometer 5010 4.52.1 Sm_V4_g.pdf
5 Mantling / Dismantling Sm_V4_h.pdf

CONTENTS
6.1 Communication protocol 6.1 Sm_V4_h.pdf

6.2 Exchange of EPROM 6.6 Sm_V4_h.pdf
6.3 DOS: Download to FLASH MEMORY 6.7 Sm_V4_h.pdf
6.4 WINDOWS: Resume after malfunction 6.9 Sm_V4_h.pdf
6.5 Initialization of bootstrap 6.12 Sm_V4_h.pdf
7 Trouble shooting 7.1 Sm_V4_h.pdf
8 Spare parts 8.1 Sm_V4_h.pdf
9.1 Calibration procedure of Photometer 5010

Subject: Cuvette temperature 9.1.1 Sm_V4_h.pdf

1 GENERAL NOTES
The data and information provided in this manual correspond to the state of knowledge existing at
the time of introducing the Photometer 5010 on the market. Any important changes will be taken
into account in the next edition of this manual.
In any case, the respective packaging leaflet should be regarded as authoritative.
This service manual was created for the telephone service and technical service staff.
1.1 Contact Partner

Technical product management and support:
ROBERT RIELE GmbH & Co KG
Address: Kurfürstenstraße 75 - 79
D - 13467 Berlin
Germany
Phone: ++49 / 30 / 404 40 87

Fax: ++49 / 30 / 404 05 29
e-mail: info@riele.de
1.2 Proved Security

This instrument has been constructed under the regulations of EN 61010-1:1993 and
EN 61010-1/A2:1995.
This device was examined and left the factory in perfect technical condition. To preserve this and
protect safe and faultless operation, the user must follow the orders and remarks of this service
manual.
To preserve this condition and to guarantee a safe working, informations and working advices,
which are included in this working instructions, have to be heeded by the applier.
This instruments meets protection 1 (earth conductor wire).
1.3 Precaution and Hazards

All electrical equipment is potentially hazardous. Never remove covers without first ensuring that it
is isolated from the AC supply, unless specific maintenance instructions or repairs are being carried
out by authorized ROBERT RIELE personnel.
The hard- and software is subject to a program of continuos evaluation and improvement and,
therefore, may be changed in the future. This also concerns service requirements.
All samples and reagents should be treated with caution accorded to those known to contain
pathogenic organisms. Similarly, the cleaning of component part of Photometer 5010 should be
done with respect to human health.
RIELE BERLIN Photometer 5010 / V4 1.1 20.08.2003

1 GENERAL NOTES
Warning: All components used on Photometer 5010 must be regarded as potentially dangerously
contaminated when doing repair work. Use rubber gloves or double gloves whenever cleaning or
sterilizing components. The most frequently used components (tubings, cuvette, measuring
chamber flap, sip. lever and waste container) should be cleaned and treated with a suitable
disinfecting solution (75% alcohol) prior to doing any service work.
Disinfect and wash hands after work is completed.
This instrument has been constructed and checked in accordance with Standards IEC 1010. When
the instrument leaves our factory it is in perfect condition from the point of view of work safety. To
keep it that way and to ensure safe operation the user must follow the instructions and warnings
given in the operation instruction manual.
The electrical protection of the apparatus corresponds to Class I (it has a protective earth).
The power plug must only be inserted into a socket that has a protective contact. The protection
must not be abolished by using an extension lead that does not have a protective earth wire.
Warning:
Any break in the earth wire inside or outside the apparatus and any loose connection of the earth
wire can make the operation of the apparatus dangerous. A deliberate break or interruption is not
allowed.
When the housing is opened or the components are being removed (except when this can be done
by hand), live parts may be exposed. Connection may also be live.
Therefore, if the carrying out of an adjustment, service or repair on the open apparatus in the live
state is unavoidable, this must be done by an expert who is familiar with the dangers involved.
Make sure you only use fuses of the specified type and rated amperage when replacing the old
ones. Repaired fuses must not be used and the fuse holder must not be bypassed.
If you have any reason to believe that the instrument can no longer operate safely, take it out of
use and make sure no one can use it accidentally.
It must be assumed that safe operation is no longer possible when the instrument:
• shows visible signs of demage
• fails to operate
• has been stored under unfavourable conditions for a fairly long time
• has been transported under rough conditions
The Photometer 5010 should be used by appropriately qualified persons only.

2 SERVICE CONCEPTS
2.1 Service Level

From the early stage of development, Photometer 5010 was designed for simple error detection
and easy exchangeability of modules. This gives the service workshops the possibility of a fast and
easy repair of the instrument on service level A (module level). No big stock or expensive
equipment is necessary and service technicians are easier to train. Also, a permanent technical
improvement in layout and components took and takes place for better productivity and economic
production.
On repairable modules the quality and function is always provided by the manufacturer according
to the latest technology. This keeps Photometer 5010 always on the highest quality level.
The exchange price for modules will be kept on a low level to guarantee repairs, on an economical
basis.
2.2 Conditions
Warranty period The warranty period for instruments is 16 months starting with the date of
for instruments ... shipment ex works Berlin/Federal Republic of Germany or 12 months
starting with the date of the first installation, whichever period is shorter.
... and spare parts For spare parts a warranty of 6 months from installation date of the part
applies. Prerequisite for this is, that the warranty period for spare parts shall
not fall short of the warranty period of the instrument where a spare part has
been installed. A further prerequisite is, that this period shall not exceed a
period of 24 months starting with the delivery of the spare part ex works
Berlin/Federal Republic of Germany.
Hint:
In case the instrument has a remaining warranty period of more than
6 months, the parts remain under warranty until the warranty period of the
instrument expires.
Handling of Replacement of parts

warranty claims The defective parts have to be handled via Return Authorization (RA)
procedure. Please answer all the questions on the RA form with the greatest
care.
Especially a detailed fault description is needed or the warranty claim will not
be accepted by the manufacturer.
Complete instruments are not accepted unless this has been agreed with
the service department of ROBERT RIELE GmbH & Co KG.
Important information:
− Only parts marked with "A" in the price list are generally accepted under
warranty.
− Only return those parts marked with "R" in the spare part list.
− All defective parts ( non-"R" and "A" parts ) should be kept on stock for
a period of 7 months. In case the manufacturer needs the part for
investigation it will be requested from Berlin.
− All parts returned to Berlin and not requested by Berlin will be sent back
at the expense of the dealer/customer.

2 SERVICE CONCEPTS
Exclusion of The aforementioned warranties do not apply in case of improper use,
warranty handling, transportation or storage, faulty installation, repair or maintenance,
Chemical influence or contamination as well as damages resulting from that,
failure to follow operating instructions, alterations or modifications of
instruments or parts thereof not authorized or recommended by
ROBERT RIELE GmbH & Co KG and resulting damages, normal wear and
tear and in case of other circumstances beyond the control of
ROBERT RIELE GmbH & Co KG.
Handling of As a general rule, all instrument repairs should be carried out by authorized
repairs and trained personel only.
Repair of parts marked with "R"

Parts which are economically worth repairing are marked with "R" in the
spare parts price list. The defective parts have to be handled via Return
Authorization procedure. For correct handling of the exchange it is
absolutely necessary to return the complete filled in RA form (preferable via
electronic mail), giving full details of the defect.
Repair of instruments
Complete instruments are not accepted for replacement or repair unless
this has been agreed with ROBERT RIELE GmbH & Co KG.
Before replacement or repair can take place, the validity of the request must
be examined and the question of costs must be settled in a written
agreement with ROBERT RIELE GmbH & Co KG.
Terms of delivery All shipping conditions for spare parts to the dealer/customer are
ex. work Berlin.
Emergency shipments require additional costs to be charged.
Emergency In case of emergency, a copy of the complete RA form should be sent by fax
shipment (++49 / 30 / 404 05 29) or electronic mail (e-mail: info@riele.de) to
ROBERT RIELE GmbH & Co KG, Berlin
marked with EMERGENCY
If fax was used for Emergency RA the original RA form has to be mailed or
sent by electronic mail to Berlin.
Handling of costs Replacements for R-parts are shipped at a repair price. In case the parts are
no longer repairable, the dealer/customer will be charged lateron with the
difference of the new price.
Replacements for warranty requests are shipped free of charge.
In case the manufacturer does not accept the warranty request, the
dealer/customer will be charged lateron with the R-price for R-parts and the
new price for non R-parts.

2 SERVICE CONCEPTS
RA form (see Return Authorization
form at next page) Please answer all the questions on the RA form with the greatest care and
sign the form.
− Date (problem date)

− Type of instrument
− Serial No. (of the instrument)
− Installation date (of instrument)
− mark Spare Part (instruments should not be returned)
− Mat.-No. of the spare part
− Fault description (incl. error code and job codes)
− mark request e.g. Repair and Replacement
− fill in RA-number
− give place and date (when filled in form) and sign RA form
Note: RA sent via electronic mail to Berlin is valid without a

signature.
In case of
instrument out of − Installation date of spare part when part was installed within the last
warranty 6 months.
All returned parts should be individually labelled with the corresponding RA

No. and shipped together with the completed RA form (if not sent via
electronic mail) to:
ROBERT RIELE GmbH & Co KG

Kurfürstenstraße 75 − 79
D-13467 Berlin
Germany

3.1 BLOCK DIAGRAM
RIELE BERLIN Photometer 5010 / V4 3.1.1 04.09.2003

3.2 SHORT INSTRUCTION GUIDE
MODE FUNCTIONS
The mode functions serve for changing definite basic settings during a course of method. It is not necessary to
exit the method and to change to the level of UTILITY PROGRAMS (OM chapter 8). Also temporary parameters
remain unchanged. Corresponding to these features this group of functions is available only within the repetition
sequence of a course of method (chapter 5.5 – DESCRIPTION OF METHOD COURSES).
Change to the selection level of the mode functions with [MODE].
MODE FUNCTIONS Selection level of the mode functions: Selecting the desired mode function by
direct selection i.e. input of the number key in question (see table) or by scrolling
through all mode functions with [+] ... [+]. Confirm with [ENTER].
Exit the selection level of the mode functions and change back to the course of
method with [BREAK].
After working off a mode function the return is carried out to the course of method.
MODE FUNCTIONS
Key/-combination Function Chapter
[MODE] [4] Parallel printers ON / OFF 5.6
[MODE] [5] Multi-standard tools 5.10
[MODE] [6] Pump utilities 5.7
[MODE] [7] Serial interface utilities 5.8
[MODE] [8] Changing sample numerator 5.9
RIELE BERLIN Photometer 5010 Service 3.2.1 22.08.2003

3.2 SHORT INSTRUCTION GUIDE
SELECTION OF UTILITY PROGRAMS

METHOD NO. Method selection: change to utility program selection with [MODE].
INPUT 1-250
Confirm with [MODE].
INPUT 1-n/+ Utility program selection: direct selection of required utility program, i.e. input of
UTILITIES concerning identity no. (see table) and confirming with [ENTER], or by scrolling
through all utility programs with [+] ... [+].
Abort of utility program selection and return to program selection with [BREAK].
A utility program can be broken off with [BREAK] at any time with return to the
selection of utility programs.
After working off a utility program automatic return to the selection of utility
programs.
Identity Utility Program Descript

ion
-No.
in
chapter
1 Dark level adjustment 8.2.1
2 Adjustment of bubble detector 8.2.2
3 Error list 8.2.3
4 Deleting method 8.2.4
5 Language selection 8.2.5
6 Beep ON / OFF 8.2.6
7 Temperature control ON / OFF 8.2.7
8 Temperature calibration 8.2.8
9 AD-Transducer 8.2.9
10 ADC dark current values 8.2.10
11 Display test 8.2.11
12 Printing device configuration 8.2.12
13 Real time clock ON / OFF 8.2.13
14 Filter wheel test 8.2.14
15 Temperature correction single cuvette 8.2.15
adaptor
16 Temperature correction flow-through 8.2.16
adaptor
17 Printing user defined methods 8.2.17
RIELE BERLIN Photometer 5010 Service 3.2.2 22.08.2003

3.3 SERVICE TOOLS
SELECTION OF SERVICE TOOLS
In the service program level there are functions influencing special basic parameters of the instrument.
Because they are allowed to be changed divergent from the work configuration only in exceptions and only by
trained staff, their unintentional selection is protected by a password.
Program selection: Change to service program selection with [MODE].

METHOD NO.
INPUT 1-250
Confirm with [LF].
Enter password [13467]. Confirm with [ENTER].

PASSWORD 13467
The service level will be opened until to the next shutdown of the device.
SERVICE TOOLS
Service tools selection: Direct selection of desired service tool, i.e. input of
INPUT 1-n/+ concerning item no. (see table) and confirming with [ENTER], or by scrolling
SERVICE TOOLS through all service tools with [+] ... [+].
Abort of service tool selection and change back to program selection with
[BREAK].
A service tool can be broken off with [BREAK] at an any time. Then a change is
carried out back to the selection of service tools.
After working off a service tool the return is carried out to the selection of service
tools.
Item no. Service tool Description

in chapter
1 System initialization S1
2 Free methods initialization S2
3 Parameter initialization S3
4 Serial number S4
5 Boost mode S5
6 Filter select S6
7 Automatic Result S7
8 Temperature functions S8
9 Block Code Character from S9
method
10 Analog-digital converter S10
correction
11 Voltages S11
12 Reduction of lamp voltage S12
13 Attribut of PCB layout S13
14 Patients data initialization S14

3.3 SERVICE TOOLS
DESCRIPTION OF SERVICE TOOLS
S1 System initialization
With this function the default parameters will be set.
Start menu by [MODE] [LF] [1]:
Confirm with [ENTER].
Following settings are then typed over in the battery powered RAM. They remain
SYSTEM INIT. S1 active, even after switching the instrument off/on, until they are specifically
SERVICE TOOLS changed. This action ends with a long beep and a print-out of the program
release.
Beeper ON
Printer internal ON
Printer external OFF
Real time clock ON
Boost mode up to 390 nm
Language english
Analog-digital converter correction 75
Temperature control: Correction values 3000
EDP OFF
EDP protocol STX/ETX/BCC
Baud rate 9600 bps
Resulting factors of methods based on standard cleared
Error list cleared
Laboratory name not affected
User name not affected
Patients data not affected
Bubble detector ON
S2 Free methods initialization

Attention! With this function all methods edited by the user are deleted without call-back. In the case of doubt
these methods should be checked before: Scroll through all methods with the method editor and note them
down for an optional later new programming.
This action ends with a long beep.
FR.METH.INIT. S2
SERVICE TOOLS

3.3 SERVICE TOOLS
S3 Parameter initialization
Attention! The extended memory for the parameters of all nonlinear methods is deleted by this function without
call-back and the corresponding user defined methods become useless.
This action ends with a beep.

PARAM. INIT. S3
SERVICE TOOLS
S4 Serial number
Display of the current serial number. A new number can be entered with max. five digits.
Confirm the input with [ENTER].

SERIAL NO. S4
SERVICE TOOLS
S5 Boost mode
To improve the low light gain at small wavelengths, the measurement time is extended when operating below
a definite wavelength. This definite wavelength will be displayed for approx. 2 sec. when starting this service
program. The default value is 390 nm.
Enter [0] = BOOST OFF or a value between 300 and 900 as definite wavelength
BOOST ON/OFF S5 in nanometers, below which the boost mode is activated. Finally confirm the input
SERVICE TOOLS with [ENTER].
S6 Filter select
The assignment of filter positions can be made.
Select filter position by numeric key. Confirm the input with [ENTER].
FILTERSELECT S6 Enter new wavelength by numeric key. Confirm the input with [ENTER].
SERVICE TOOLS Finish the operation with [BREAK].

3.3 SERVICE TOOLS
S7 Automatic Result
With this service program a continuous measuring mode based on calculation procedure 1 (endpoint with
factor) is possible. After the input of wavelength, factor and delay-time and after measuring blank the con-
tinuous measuring mode is started.
This mode is active until [BREAK] is pressed (and held for a while).
AUTO. RESULT S7
SERVICE TOOLS
S8 Temperature functions
A sub menu with eight items for temperature functions is called.
Direct selection of desired service tool, i.e. input of concerning item no. and
TEMP. FUNCT. S8 confirming with [ENTER], or by scrolling through all temperature functions
SERVICE TOOLS with [+] ... [+].
Item no. Service tool Description

in chapter
1 Temperature regulation on / off S8.1
2 Temperature value of standard S8.2
cuvette adaptor
3 Temperature value of S8.3
flowthrough cuvette adaptor
4 Temperature calibration S8.4
5 Temperature required / actual S8.5
6 Temperature Analog-Digital- S8.6
Converter

3.3 SERVICE TOOLS
S8.1 Temperature regulation on / off

The temperature regulation can be switched on or switched off. In the display the current setting is shown.
Start menu by [1]:
TEMP. ON/OFF
INPUT +/E/B
Changing of the setting with the keys [1] = ON or [0] = OFF.

The displayed setting becomes active with [ENTER] and [BREAK].
HEATING OFF
1/ON 0/OFF
Select temperature level by [1], [2] or [3] in case of setting ON.
HEATING ON
1/25 2/30 3/37
S8.2 Temperature value of standard cuvette adaptor

Display of the current setting. The correction of the temperature regulation is possible by change of the
temperature setting.
Start menu by [2]:
E-TEMP. VALUE
INPUT +/E/B
Select temperature level by [1], [2] or [3].

E-TEMP. VALUE
1/25 2/30 3/37
Enter new temperature value and confirm with [ENTER]

37C VALUE E or finish operation with [BREAK]. The window shows for example the value 13047
13047 for the temperature level 37 °C.
S8.3 Temperature value of flowthrough cuvette adaptor

Display of the current setting. The correction of the temperature regulation is possible by change of the
temperature setting.
Start menu by [3]:
D-TEMP. VALUE
INPUT +/E/B
Select temperature level by [1], [2] or [3].

D-TEMP. VALUE
1/25 2/30 3/37
Enter new temperature value and confirm with [ENTER]

37C VALUE D or finish operation with [BREAK]. The window shows for example the value 13047
13047 for the temperature level 37 °C.

3.3 SERVICE TOOLS
S8.4 Temperature calibration

The correction of the temperature regulation is possible by input of the temperature which is measured in the
cuvette with an external measuring system (only when the temperature regulation is stabilized).
Start menu by [4]:
TEMP. CALIBR.
INPUT +/E/B

The display shows the current temperature level at the required value of 37 °C.
INPUT DEGREE C Enter the actual temperature value always four-digit in °C e.g. [3] [7] [,] [2] [0]
37C 37.20 [ENTER] measured by an external measuring system. The system calculates and
stores the new setting for 37.0 °C and starts working with it at once.
S8.5 Temperature required / actual

The correction of the temperature regulation is possible by input of the temperature which is measured in the
cuvette with an external measuring system (only when the temperature regulation is stabilized).
Start menu by [5]:
TEMP. REQU / ACT
INPUT +/E/B

TEMP. REQU / ACT The display shows in the second line the required value (e.g. 14270),
14270 14275 D the actual value (e.g. 14275) and the type of cuvette adapter (e.g. D).
S8.6 Temperature Analog-Digital-Converter

Display of the counter value of the temperature ADC and of the current stability value.
Start menu by [6]:
TEMP. ADC
INPUT +/E/B

TEMP. ADC The display shows in the second line the actual value of the ADC (e.g. 954),
ADC 954 S 0 and the stability value S (e.g. 0).

3.3 SERVICE TOOLS
S9 Block Code Character from method

To check the correct storing of a user-defined method, the special test byte BCC concerning the data in the
memory is used. With this service program the BCC of a method can be determined.
The number of the concerning method must be entered and confirmed with
BCC FROM METH.S9 [ENTER]. Then the BCC is displayed in the decimal form.
SERVICE TOOLS Press [ENTER] and select another number of a method.
Press [BREAK] for exit.
S10 Analog-digital converter correction

Display of the actual correction value of the analog-digital converter (photometric measuring system). In the
delivering condition the value is 75.
By input of a correction value between 0 and 255 and confirming with [ENTER]
ADC CORRECT.S10 measurements seeming too low can be increased a little bit. This correction
SERVICE TOOLS recognizably affects only at high results (~ 2 Absorbance).
S11 Voltages
Display of the actual voltages on the pcb.

VOLTAGES S11
SERVICE TOOLS
The display shows the actual voltages of

12.03 12+0.07 + 12 VDC of halogen lamp / fan (1. line left)
-4.93 14.75 0.01 + 12 VDC and - 12 VDC of analogic circuits (1. line right)
+ 5 VDC of VCC (2. line left)
+ 15 VDC of power supply (2. line middle)
VDC voltage of Peltier element (2. line right)

3.3 SERVICE TOOLS
S12 Reduction of lamp voltage

The voltage + 12 VDC of halogen lamp can be reduced for lamp adjustment in vertical direction.
Confirm with [ENTER]. Adjust lamp in vertical direction.
LAMP DOWN S12
SERVICE TOOLS Press [BREAK] for exit.
S13 Attribut of PCB layout

The attribut of the PCB layout can be read and set. Software applications may be dependent on this attribut.
The attribut is labeled on the PCB RR_111 near plug ST13 (RS232C interface).
PCB LAYOUT S13
SERVICE
c TOOLS+EB
In the second line the display shows the version of PCB layout, e.g. the value „c“.
Change the value by [+] in case of a deviation and confirm the input by [ENTER].
Leave the window by [BREAK] in case of no change.
S14 Patients data initialization

The memory of patients data mangement is cleared completely.
The memory is cleared by confirming with [ENTER].
DEL.PATIENTS S14
SERVICE TOOLS This action ends with a long beep.

3.4 PLUG IDENTIFICATION
In the device all plug-in connections are marked with P1 ... P11 as described below:
PLUG FROM ⇒ TO
• P1 AC / DC power supply ⇒ motherboard pcb RR-115
• P2 motherboard pcb RR-115 ⇒ halogen lamp
• P3 motherboard pcb RR-115 ⇒ fan
• P4 motherboard pcb RR-115 ⇒ Peltier element
• P5 motherboard pcb RR-115 ⇒ motor peristaltic pump
• P6 motherboard pcb RR-115 ⇒ motor filter wheel
• P7 motherboard pcb RR-115 ⇒ cuvette connection RR-103-V4
• P8 motherboard pcb RR-115 ⇒ photodiode
• P9 motherboard pcb RR-115 ⇒ display unit RR-114
• P10 motherboard pcb RR-115 ⇒ printer internal CN4
• P11 motherboard pcb RR-115 ⇒ printer internal CN2

4.1 MAINS POWER CIRCUIT
Function Description
The mains power circuit shows the design of the complete primary circuit. Components of the circuit are:
• Mains connector
• Mains fuses F1 and F2 ID 1707353001
• Mains switch
• AC/DC power supply ID 5010-016
• Line filter
• Ground connection
• Wires
Location
Backside of Photometer 5010, basis plate
Replacement
The Photometer 5010 has to be opened. The top case has to be separated from the bottom case.
See chapter 4.3 for replacement of AC/DC power supply.
Installation
For installation proceed in reverse order.
See also exploded view, chapter „Dismantling“
Fuses
Between the mains switch and the mains cable connector there is a cartridge with two fuses F1 and F2 with
following specifications:
• dimensions [mm] : 5 * 20
• standard: IEC 60127-2/V
• time-current characteristic: time lag (T)
• voltage rating: 250 V
• rated current: 1.6 A
• marking: T 1.6 A H

4.1 MAINS POWER CIRCUIT
power input
90 V~ - 264 V~
gnye gnye
fuses F1 and F2
ground
connection
wh gr
mains switch
br bk
line filter
FG N L
AC / DC
power supply

4.2 MAINS CONNECTOR
The unit connects the power input with the AC/DC power supply.
Location
Backside of Photometer 5010, basis plate
fan
mains switch
fuses F1 and F2
power input
90 V~ - 264 V~
waste tubing

4.3 AC / DC POWER SUPPLY ID 5010-017
The AC / DC power supply is designed for producing following voltages:
• V1: + 5 VDC / 7 A
• V2: + 15 VDC / 3 A
• V3: - 15 VDC / 1 A
Location
Basis plate of Photometer 5010
Replacement
Installation
Terminal block

4.3 AC / DC POWER SUPPLY ID 5010-017
power input
90 V~ - 264 V~
P1
line filter
ground
connection
P1
blue red
violet pink
+15V/GND/+5V/-15V

4.10 OPTICAL UNIT
The optical unit consists of the following components:
• Filter wheel with filters
• Step motor for filter wheel
• Light barrier filterwheel
• Tooth belt
• Halogen lamp
• Photodiode
• Lenses
• Step motor for peristaltic pump
• Cuvette connection pcb RR-103-V4
Location
Basis plate
Replacement
Remove the lamp shield. The lamp shield is fixed with two screws at the bottom case.
Loosen four screws A (see page 4.10.2).
Loosen one screw B near the step motor of filter wheel (see page 4.10.2).
Unplug the connectors:
• P4 Peltier element
• P5 Peristaltic pump
• P7 Cuvette connection pcb RR-103-V4
• P8 Photodiode
Installation
bottom case
lamp shield

4.10 OPTICAL UNIT
A A A
A

4.11 FILTER WHEEL ID 5010-004
The filter wheel has eight filter positions. Six filters with standard wavelengths are equipped.
The wavelengths are: 340 nm, 405 nm, 492 nm, 546 nm, 578 nm and 623 nm
Location
Optical unit
Replacement
Remove optical unit (see chapter 4.10)
Remove filter wheel axle and filter wheel.
Installation
White mark
of filterglass
340 nm
405
340 546
578
#8 492
#7 623
6
8
54
57
5
40
2
49
White mark
of filterglass
0
34
3
62
340 nm
#7
#8

Installation of additional filters
The filter wheel can be equipped with additional optical filters in the positions 1 to 8.
In the standard equipment the not used positions are optically locked by metal slices.
The respective metal slice is replaced with a filter at installation of an additional filter. In the memory of the
Photometer 5010 the value of the wavelength (e.g. 670) for the corresponding position (e.g. 7) becomes
changed by a service software routine.
Mechanical operations
Location
Optical unit
Replacement
Open the Photometer 5010. Separate the top case from the bottom case.
The filter wheel is turned by hand so that the corresponding hole is at the cut-out of the motor fastening plate
(see page 4.10.2).
With the Tool kit filter exchange ( RIELE ID-No. 5010-001 ) a filter exchange can be easily carried out. The
Tool kit filter exchange consists of the tools A and B.
Tool B
pipe wrench
Tool A
With the tool A (left illustration) and a pipe wrench the metal slice can be pushed from the hole in the filter
wheel. The cheeks of the pipe wrench grasp at the tool and at the filter wheel. With a screwdriver the Seeger
ring and the metal slice can be taken.
Set the filter glass into the corresponding hole of the filter wheel. If the filter has only one reflecting surface,
this reflecting surface must be judged to the light source. The Seeger ring can loosely be inserted into the hole
in the filter wheel.
With the tool B (left illustration) and a pipe wrench the filter glass and the Seeger ring can be fixed.
Service utilities
1. Connect device with the mains and switch it on.
2. If the display shows error messages:
Confirm them with [ ↵ ]
3. The display shows:
METHOD NO.
INPUT 1-250

4. Press [MODE], [LF] for entering service level
PASSWORD
SERVICE TOOLS
5. Enter password [13467] for entrance into service level

PASSWORD 13467
SERVICE TOOLS
6. Press [ ↵ ]
7. The instrument is ready for operations in service level and the display shows:
INPUT 1-n/+
SERVICE TOOLS
8. Press [7], [ ↵ ] for editing wavelength

FILTERSELECT S7
SERVICE TOOLS
9. Press [ ↵ ]
10. Select corresponding hole ( e.g. 7 ) of the filter wheel with [ + ] and [ ↵ ]
FILTER
F7 999NM +-#
9. Enter the value of the new wavelength ( e.g. 670 )

FILTER
F7 999NM 670#
11. Press [ ↵ ]
12. The display shows:
FILTER
F7 670NM +-#
13. Press [BREAK], [BREAK]

14. Press [MODE], [MODE], [ 9 ], [ ↵ ], [ ↵ ], [ ↵ ] for control of ADC-counter value in corresponding filter
position (OM chapter 8.2.9 / AD-Transducer measurands).
Select corresponding hole ( e.g. 7 ) of the filter wheel with [MODE], [ 7 ].
The ADC-counter value should be within a range of 3.000 and 600.000.
ADC COUNTS 9
0 327180 F7
15. Finish the operation with [BREAK]

4.12 HALOGEN LAMP ID 0554871001
The halogen lamp is working with 12 VDC and has an electrical power of 20 W.
Location
Optical unit
Replacement
Loosen the two screws for mounting of halogen lamp.
Remove the halogen lamp.
Installation
Insert the halogen lamp. Do not touch the glass of the halogen lamp with fingers.
Use the plastic foil within the original packing of the spare part.
The socket of the halogen lamp should be mounted horizontally with direct contact to the lamp holder.
Tighten the two screws for mounting of halogen lamp.
Install the lamp shield.
Switch on photometer 5010.
The display shows:

METHOD NO.
INPUT 1-250
Press [MODE], [MODE], [ 9 ], [ ↵ ], [ ↵ ], [ ↵ ] for control of ADC-counter value in corresponding filter position
(OM chapter 8.2.9 / AD-Transducer measurands).
Check one after the used positions of the filter wheel.
Select corresponding hole ( e.g. 2 ) of the filter wheel with [MODE], [ 2 ].
The ADC-counter value should be within a range of 3.000 and 600.000.
ADC COUNTS U9
0 327180 F2
The halogen lamp has to be adjusted horizontally by the adjustment screw to maximum value of ADC.
Finish the adjustment with [BREAK].

4.12 HALOGEN LAMP ID 0554871001

4.13 PHOTODIODE WITH PLUG ID 1707388001
A photodiode is used as receiver, which gives an electrical signal depending on received light.
Location
The photodiode is situated at the optical unit. The Plug P8 ist connected with the plug ST8 on the mother
board RR-115.
Replacement
Unplug the photodiode from the mother board RR-115.
Remove the two screws (B).
Remove the adjustment piece (C) of the holder.
Installation
P8
Shield A 1
2
wh K 3
Photodiode
K A with Anode (A) and Kathode (K)
bottom view

4.14 HEATING UNIT ID 1707612001
As heating unit a power darlington transistor is used located at the optical unit. The transistor is delivered with
plug.
Location
Optical unit.
Replacement
Remove the optical unit (see chapter 4.10).
Remove 4 screws of cuvette adaptor well with heating unit and 2 screws between cuvette adaptor well and
heating unit.
Loosen screw of heating transistor.
Installation
TIP 132
yellow
thermal compound
violet
orange
P4

4.15 PELTIER ELEMENT ID 1707370001
A peltier element is used to cool the cuvette adaptor.
Location
Optical unit
Replacement
Loosen 4 screws of cuvette adaptor well with heating unit and 2 screws between cuvette adaptor well and
heating unit.
Loosen 2 clamp screws (glued) of Peltier element
Installation
Take care that the 2 clamp screws are equally tightened. Put one drop screw glue on each thread tip.
Put heat conducting paste on the Peltier element.
Clamp Peltier element only so tight that it cannot be moved by hand.
ld
co
black
red
P4

4.16 PUMP MOTOR WITH PLUG ID 5010-017
The peristaltic pump is used for sample aspiration into the flow-through cell. A step motor drives the rotor of
the pump.
Location
Optical unit
Replacement
Remove optical unit (see chapter 4.10).
Remove pump wheel with clamp screw (alan key).
Loosen 4 screws of pump motor.
Installation
gr 4
gr 3
gr 2
ye 1
P5

4.17 TOOTH BELT ID 1707558001
The tooth belt is used for driving the filter wheel.
Location
Optical unit
Replacement
Remove filter wheel axle and filter wheel.
Remove tooth belt.
Installation
Adjust belt tension by moving step motor.
3 - 4 mm
#7
#8 623
492
340 578
405 546

4.18 STEP MOTOR FILTER WHEEL ID 1707540001
The step motor is used for driving the filter wheel.
Location
Optical unit
Replacement
Disconnect plugs P2 and P6 of mother board.
Remove lamp/ filter wheel unit with 2 screws.
Remove lamp unit from filter wheel plate with 3 more screws.
Open cable tree and disconnect motor.
Remove motor with screws.
Installation
Take care of belt tension (see 4.17).
8 7 6 5 4 3 2 1 P6
gn
rd
vio
wh
bl
bl
wh
Motor
Filterwheel
Light barrier
+ D Top view of
E + light barrier

4.19 LIGHT BARRIER FILTER WHEEL ID 1707213001
A light barrier is used to control positioning of the filter wheel.
Location
Optical unit
Replacement
Disconnect plugs P2 and P6 of mother board.
Remove lamp/ filter wheel unit with 2 screws.
Remove filter wheel and tooth belt with filter wheel axle.
Remove light barrier from preadjusted mounting.
Open cable tree and disconnect light barrier.
Installation
8 7 6 5 4 3 2 1 P6
gn
rd
vio
wh
bl
bl
wh
Motor
Filterwheel
Light barrier
+ D Top view of
E + light barrier

4.20 BUBBLE DETECTOR WITH PLUG ID 1706870001
The bubble detector controls the rotations of the peristaltic pump means the aspirated volume.
Location
Optical unit
Replacement
Disconnect plug of detector.
Installation

4.21 FLOW-THROUGH CUVETTE ADAPTOR ID 1704796001
The flow-through cuvette adaptor consists of:
• Plastic adaptor
• Flow-through cuvette with a volume of 32 µl
• Heating unit
• Plug
• Potentiometer for factory calibration
Location
Optical unit
Replacement
Loosen clamp screw, open tube connection, remove adaptor.
Installation
After installation of a new cuvette adaptor or a change e.g. from the standard cuvette adaptor to the flow-
through cuvette adaptor a dark level adjustment with [MODE] [MODE] [1] is recommended.

4.22 STANDARD CUVETTE ADAPTOR ID 1704800001
The standard cuvette adaptor is used for disposable cuvettes.
The standard cuvette adaptor consists of:
• Plastic adapter
• Heating unit
• Plug
• Potentiometer for factory calibration
Location
Optical unit
Replacement
Loosen clamp screw, remove adaptor.
Installation
After installation of a new cuvette adaptor or a change e.g. from the flow-through cuvette adaptor to the
standard cuvette adaptor a dark level adjustment with [MODE] [MODE] [1] is recommended.
holding spring for disp. cuvette

ID 1707159001

4.23 CONNECTOR BUBBLE DETECTOR ID 1706900001
The connector bubble detector connects the bubble detector with cuvette connection pcb.
Location
Optical unit
Replacement
Disconnect plug P2 of cuvette board.
Loosen milled nut and remove connector bubble detector.
Installation
grey
violet P2
blue

4.24 LAMP HOLDER ID 1707205001
The lamp holder is used for fixing the halogen lamp.
Location
Optical unit
Replacement
Loosen clamp screw (alan key) and remove holder with lamp.
Installation

4.25 CUVETTE LENSE KIT ID 1706926001
Location
At the light entrance side of cuvette adaptor
Replacement
Flow-through adaptor: O-ring, lense, snapring SB12
Disposable cuvette adaptor: lense, snapring SB12
Installation
O - ring
Lense
Snapring SB12

4.26 TUBES
Waste tubing Pump tube with joints

ID 1704834001 ID 5010-013
Outlet tube cuvette

ID 5010-015
Aspirate tubing
ID 5010-014
Joint inlet tube cuvette

ID 1707175001

4.26 TUBES
Waste tubing
ID 1704834001
Aspirate tubing
ID 5010-014
Joint inlet tube cuvette

ID 1707175001
Outlet tube cuvette Pump tube with joints

ID 5010-015 ID 5010-013
Aspirate tubing ID 5010-014
Joint inlet tube cuvette ID 1707175001
Outlet tube cuvette ID 5010-015
Pump tube with joints ID 5010-013
Waste tubing ID 1704834001

4.30 TOP CASE ID 5010-008
The top case is one of the two main parts of the housing. It is assembled with:
see
• Display glass 4.31
• Microswitch 4.33
• Keyboard 4.34
Location
Top case
Replacement
For dismantling of see

• Metal tube inlet 4.32
• Printer mechanism 4.35
• Display unit 4.42
• Printer controller 4.44
Sipping lever:
Remove pin from sipping lever.
Loosen 2 screws of bearing holding the metal tube inlet and remove both.
For installation into new top case proceed in reverse order.
Sliding lid:
Loosen guide rail at the top case and remove it together with sliding lid.
Remove spring bearing.
For installation into new top case proceed in reverse order.
Important: Be informed spring tension is factory adjusted to a certain force.
Special tool and adjustment gauge is necessary.
Installation

4.31 DISPLAY GLASS ID 1706934001
The display glass protects the LCD against mechanical damaging and liquid spill over.
Location
Top case
Replacement
Remove display unit (see 4.42)
Remove old display glass and clean frame from remaining glue.
Installation
Glue new display glass (use for example glue „Delomet Typ 2301“)
Display glass

4.32 METAL TUBE INLET ID 1703230001
The metal tube inlet is used to guide the aspirate tubing.
Location
Top case
Replacement
Remove aspirate tubing from metal tube inlet.
Remove metal tube inlet by turning and pulling at the same time.
Installation
Metal tube inlet
7 8 9 MODE LF
SERIAL NUM. SETUP
4 5 6 - +
PARAL EL EDIT
1 2 3 METHOD ZERO
0 WASH RESULT

4.33 MICROSWITCH ID 1707248001
The microswitch is pressed by the sipping lever, its signal starts the peristaltic pump.
Location
Top case
Replacement
Disconnect plug P2 of display unit.
Remove microswitch by a screw driver and clean frame from remaining glue.
Installation
Use for example glue „Delomet Typ 2301“.
gr
wh
P4

4.34 KEYBOARD ID 5010-002
The keyboard consists of 20 keys.
The top layer is made of polyester. The keyboard is self adhesive.
Location
Top case
Replacement
Disconnect ground and plug P3 of keyboard.
Remove keyboard carefully.
Note: When keyboard is removed from instrument it might not be reusable.
Installation

4.35 PRINTER MECHANISM ID 1223003001
The printer prints texts and graphics.
Location
Top case
Replacement
Remove top cover for printer, paper and ribbon.
Disconnect 18-pin plug CN16 from printer-controller-pcb.
Loosen 2 front screws in the hollows and remove the other 2 screws.
Remove printer mechanism.
Installation
Solder printer connection cable (ID 1707655001) to printer mechanism (ID 1223003001). Take care not to
solder 3 wires which are not used (see drawing). Also care for blue mark.
PUSH
EJECT
blue

4.40 MOTHER BOARD RR-115 ID 5010-115
The mother board is the central electronical unit within Photometer 5010.
Following components are equipped on the pcb:
• multiprocessorsystem based on microcontrollers SAFC517 and 87C51/52

• battery powered ram
• battery powered real time clock
• adc for photocurrent
• adc for temperature control
• stepper motor control for peristaltic pump
• stepper motor control for filter wheel
• interface infrared bubble detector
• interface RS-232C
• interface internal printer (Centronics)
• interface LCD
• interface Peltier element
• interface heating unit
• halogen lamp regulator and interface
• fuses F1, F2 and F3
Location
Basis plate
Replacement
The basis plate has to be separated from the bottom case.
Disconnect all plugs from the pcb. The plugs are labled with ST1 up to ST11.
Remove five screws fixing the pcb with basis plate.
Installation
Use heat conductor paste for getting cooling effect.
See also exploded view in chapter 5 Mantling / Dismantling.
Continue with installation instruction at the end of this chapter in case of changing a board.

Component diagramm

Voltages
1. Before adjustment switch off Photometer 5010. Open the instrument. Open the photometer regarding
safety rules of chapter 1 of this service manual.
2. Switch on instrument.
3. Use DVM for measuring ± 12 VDC.
4. Connect the negative lead of DVM to the ground connector (soldering connector).
5. Connect the positive lead of DVM to the cathode of diode D7.
6. Check the voltage + 12 VDC (tolerance ± 5%) at C13.
7. Connect the positive lead of DVM to the anode of diode D6.
8. Check the voltage - 12 VDC (tolerance ± 5%) at R59.
9. Connect the positive lead of DVM to the pin 11 of plug ST17.
10. Check the voltage + 5 VDC (tolerance ± 5%).
11. Connect the positive lead of DVM to the battery BT1.
12. Check the voltage + 3 VDC (tolerance ± 10%).
+ 3 VDC ± 10%
+ 5 VDC ± 5% Ground connector + 12 VDC ± 5% - 12 VDC ± 5%

Voltage 12 VDC of halogen lamp
3. Use DVM for measuring 12 VDC.
4. Connect the negative lead of DVM to the ground connector (soldering connector).
5. Connect the positive lead of DVM to the plus lead of the halogen lamp holder (red line).
6. Check the voltage +12 VDC (tolerance ± 100 mVDC).
7. If necessary adjust with potentiometer P1.
Turning clockwise increases voltage.
Turning counter-clockwise decreases voltage.
Potentiometer P1
Fuses F1, F2, and F3

Name Protection Specification
F1 VCC (+ 5 VDC) / Peltierelement T 6.3 A / 250 V
F2 + 15 VDC / + 12 VDC T 3.15 A / 250 V
F3 Power supply printer + 5 VDC T 6.3 A / 250 V

Amplifier for photocurrent and ADC
The photocurrent of the photodiode is converted with shortcircuit application into a voltage signal with the aid
of the amplifier IC11 (AD 711). Potentiometer P4 sets the amplification and potentiometer P3 sets the offset
voltage of the amplifier stage.
An voltage / frequency converter IC13 (AD654) is connected to the output of the amplifier.
The microcontroller can set the amplification of the amplifier stage by the multiplexer IC12 (ADG428). The
eight different settings of amplification are called V0 up to V7. The setting V0 is equivalent to the lowest
amplification.
The micocontroller converts the frequency signal of the voltage / frequency converter to a digital result in a
range between 0 and ~ 600.000. This result is called value of the ADC.
The value of the ADC in the case of photocurrent = 0 (darkness) is called offset. The offset is
• dependent on the setting of the amplifier stage.

• dependent on the used cuvette adapter
• must be positiv and <> 0.
• is set with potentiometer P3.
The following diagramm shows the ADC-counter values versus the photocurrent.
ADC
~ 600.000
offset
0
photo current

Adjustment of amplifier stage
1. Before starting adjustments switch off photometer 5010 and unplug from line. Open the photometer
regarding safety rules of chapter 1 of this service manual.
2. Insert standard cuvette adaptor for disposable cuvettes into the instrument, but do not insert cuvette.
3. Put the top case at the right side of the bottom case. Meanwhile all plugs remain plugged. Switch
instrument on.
4. Be sure that the optical unit is adjusted faultless (see chapter 4.10) and check the supply voltages and
the lamp voltage (see chapter 4.40.3 and 4.40.4).
5. Use utility AD-Transducer measurands (see OM 8.2.9)
6. METHOD NO. Start menu by [MODE] [MODE] [9]:

INPUT 1-250
7. ADC COUNTS U9 Confirm with [ENTER].

UTILITIES
8. BOOST OFF Boost operation is deactivated. Accept the displayed status with [ENTER].
1/ON 0/OFF
9. ADC COUNTS U9 On the second line the current sensitivity level V (e.g. V0) in the range 0...7
0 23711 F1 is shown on the left and the actual AD-transducer value (e.g. 23711) to the
middle. On the right the actual filter position (e.g. F1) is shown.
The current sensitivity level can be changed by numerical keys [0] up to [7].
If the current sensitivity level should be different from 0, press the key [0] up
to the beep.
The filter position can be changed by [MODE] and a numerical keys [1] up
to [9].
Filter position 1 up to 8 are used for interference filters.
Filter position 9 is used for dark position of filterwheel.
10. Amplification: Turn the filter wheel to that position, so that the shown AD-transducer value becomes
maximum. Adjust the AD-transducer value to 500.000 (± 50.000) counts with the potentiometer P4 on
the mother board. In case of not reaching this value, turn the potentiometer P4 clockwise (increasing) to
the maximum. Then reduce the amplification with turns counter-clockwise (decreasing) until AD-
transducer value is changing.
11. Offset: Switch off Photometer 5010 and change standard cuvette adaptor with flow-through adaptor.
12. Connect the negative lead of DVM to analog ground.
13. Connect the positive lead of DVM at measuring point MP1.
14. Switch on Photometer 5010.
15. METHOD NO. Start menu by [MODE] [MODE] [9]:
INPUT 1-250
16. ADC COUNTS U9 Confirm with [ENTER].

UTILITIES
17. BOOST OFF Boost operation is deactivated. Accept the displayed status with [ENTER].
1/ON 0/OFF

18. ADC COUNTS U9 Select current sensitivity level V7 by numerical keys [7].
7 17933 F9
19. Interrupt the light path by turning the filter wheel into dark position F9. The filter position can be changed
by [MODE] [9].
20. Set the DVM to the smallest voltage range.
21. Adjust the voltage to 0.0 mVDC with potentiometer P3.
22. Check all sensitivity level V0 up to V7. The AD-transducer values shall increase or decrease step by
step. For example valid values for the increasing case could be:
V0: 16865, V1: 16869, V2: 16872, V3: 16887, V4: 16899, V5: 16901, V6: 16923, V7: 16933
The deviation between V0 and V7 shall be smaller than absolute 200 points.
23. Finish the operation with [BREAK].
Ground connector P4 P3 MP1

Contrast of LCD
3. Check the contrast of LCD.
4. Adjust contrast with potentiometer P5 if necessary.
Potentiometer P5

Temperature control
General Description
The temperature control takes care for the correct temperature inside the flow-through cuvette or inside the
disposable cuvette adaptor, i.e. 25, 30 or 37 degrees Centigrade, selectable by the program.
The temperature control circuit consists of:
• microcontroller system
• temperature sensor
• analog-digital-converter (ADC)
• heating unit
• cooling unit
Microcontroller System
The temperature control circuit is a PID-controller, a software module in the microcontroller system. The PID-
controller works in the background all the time. Dependent on the cuvette adaptor the PID-controller uses
different parameters.
The required value of temperature is ascertained by the program of the microprocessor system. The
temperature is controlled according to the set temperature in the selected method.
The PID-controller gives information to the higher program about the given temperature tolerances and
therefore about the readiness for measuring.
Temperature Sensor and ADC

The voltage signal of the temperature sensor LM335 is converted by the IC2 (LTC1286) to an equivalent
frequency. The microcontroller system IC3 (8031PLCC) converts the frequency to a digital value.
Heating
The control variable of the controller has influence on the heating. A switched Darlington transistor in source of
power-switching is used as heating element. The PID-controller produces a voltage at the emitter of the
Darlington transistor by a pulse-width-modulation. This voltage controls the current through the transistor.
Involved components are the microcontroller system IC3 (8031PLCC), the OP-booster IC1A (LM324) and the
MOS-Fet T3 (BS170).
Cooling
For cooling or counter cooling during heating the microcontroller system switches on a Peltier element. There
are four possible levels with following terms and meaning:
0 = Cooling out
1 = Cooling (low level)
2 = Cooling (middle level)
3 = Cooling (high level)
In case of temperature changing by method selection cooling level 0 is selected if desired temperature is
higher as selected one.
In case of temperature changing by method selection cooling level 3 is selected if desired temperature is lower
as selected one.

If selected temperature is reached it is switched over to a cooling level depending on the temperature:
• for 37 degrees Centigrade ⇒ cooling level 1
The cooling element is a Peltier element. The Peltier element functions as a heat pump and transports heat
out of the cuvette adaptor to the heat sink. The heat sink is provided with air by the fan of the device and
transports the heat outside to the area.
The Peltier element operates with a voltage, switchable on four levels:
• cooling level 0 ⇒ 0 VDC
• cooling level 1 ⇒ 0.5 VDC
• cooling level 2 ⇒ 1 VDC
• cooling level 3 ⇒ 1.65 VDC
Involved components are the microcontroller system IC3 (8031PLCC), the OP-booster IC1B (LM324), the
transistor T1 (BD679), the transistor T2 (BD243 with cooling body), the transistor T4 (BC547) and the Peltier
element.
Adjustment of temperature control

The voltage signal of the temperature sensor LM335 is at temperatures:
• 25 degrees Centigrade typical 2.98 VDC
This means a change of 10 mVolt indicates of temperature change of one degree Centigrade.
Because of tolerances of the temperature sensor LM335 following can be measured at the measuring
amplifier:
• 25 degrees Centigrade ⇒ ≥ 2.92 VDC
• 37 degrees Centigrade ⇒ ≤ 3.16 VDC
The ADC converts the input voltage to a digital value between 0 and 32767 as shown on following diagram:
ADC
32767
ca. 17000
ca. 15200
ca. 13900
0
2,98 VDC 3,03 VDC 3,1 VDC
input voltage
of temperature sensor

Thermal adjustment
The PID-controller algorithm works always as follows:
ACTUAL VALUE = REQUIRED VALUE
REQUIRED VALUE = TEMPERATURE VALUE + CORRECTION VALUE
Explanation:
ACTUAL VALUE ⇔ Actual value of the ADC
REQUIRED VALUE ⇔ Sum of TEMPERATURE VALUE + CORRECTION VALUE
TEMPERATURE VALUE ⇔ Value assigned to a temperature level of 37 °C, 30 °C or 25 °C. This
value is automatically selected by the device according to the state of
operating. The temperature value is board-characteristic and cuvette
adaptor specific. The values for the temperature levels of 37 °C, 30 °C
or 25 °C are ascertained in factory and noted during the check of the
board. They are stored within the battery powered RAM.
Dependent on the used cuvette adaptor different service programs can
show and edit the values stored in battery powered RAM. The system
can be adjusted to lower or higher temperature values. A change of
about 25 counts means a temperature change of 0.1 °C.
• Standard cuvette adaptor:
Service program S8 (TEMP. FUNCT) /
item 2 (E-TEMP. VALUE)
• Flow-through cuvette adaptor:
Service program S8 (TEMP. FUNCT) /
item 3 (D-TEMP. VALUE)
• Service program S8 (TEMP. FUNCT) /
item 4 (TEMP. CALIBR.) calibrates the system
automatically.
RR_115 5001 The values are noted on a sticker. The sticker is shown on the left and
put on top of the IC15 (SABC517).
S FTH The sticker shows the serial serial number of the board (e.g. 3501) and
25 °C 10487 10325 the temperature values of 25 °C, 30 °C, 37 °C of standard cuvette
adaptor (S) and flow-through adaptor (FTH).
30 °C 11667 11628
37 °C 13367 13420 In addition the values are documented in the instrument card of
Photometer 5010 and stored in a file at the manufacturer.
CORRECTION VALUE ⇔ Correction value in the range of 0 and 9999. The default value is 3000.
After using the service program S1 (System init.) all correction values
are set to the default value 3000.
Dependent on the used cuvette adaptor different utility programs can
show and edit the values stored in battery powered RAM. The system
can be adjusted to lower or higher temperature values. A change of
about 25 counts means a temperature change of 0.1 °C.
• Standard cuvette adaptor:
Utility program U15 (E-CUV. CORR.)
• Flow-through cuvette adaptor:
Utility program U16 (D-CUV. CORR.)
• Utility program U8 (TEMP. CALIBR.) calibrates the system
automatically.

Installation instruction
In case of changing the mother board following items have to be done after switching on the device:
1. Check the contrast of LCD.
See Service Manual chapter 4.40 – Contrast of LCD.
2. Check the voltage 12 VDC of halogen lamp. The voltage was preadjusted at factory.
See Service Manual chapter 4.40 – Voltage 12 VDC of halogen lamp.
3. Check voltages + 12 VDC, - 12 VDC, + 5 VDC and + 3 VDC (battery power).
See Service Manual chapter 4.40 – Voltages.
4. Perform the adjustment of amplifier stage.
See Service Manual chapter 4.40 – Adjustment of amplifier stage.
5. The display of Photometer 5010 shows:
METHOD NO.
INPUT 1-250
6. The serial number can be set by applying the service tool S4.
See Service Manual chapter 3.4 - S4 Serial number.
7. The assignment of filter positions has to be done if additional filters has been built in
(e.g. 670 nm). Apply the tool S6.
See Service Manual chapter 3.4 - S6 Filter select.
8. Do a setting of temperature values by performing the calibration procedure of Photometer 5010 /
Subject: Cuvette temperature.
See Service Manual chapter 9.1
9. Do a setting of “attribut of PCB layout“ by applying the service tool S13:
Software applications may be dependent on this attribut.
See Service Manual chapter 3.4 - S13 Attribut of PCB layout.
10. Do a “patients data initialization“ by applying the service tool S14:
Attention! The memory of patients data mangement is cleared completely.
In case of a migration to operating system version 3.5 the operation has to be done.
11. Define the laboratory name by applying the utility U18:
The name of the laboratory can be stored permanently.
12. Define the user names by applying the utility U19:
The names of maximum five users can be stored permanently.
13. Do a dark level adjustment by applying utility U1 in combination with flow-through system.
See OM chapter 8.2.1 - Dark level adjustment.
The utility is working specifically to the current adaptor. An adjustment of the standard cuvette adaptor
should be done later on.
14. Do a media test by applying utility U2.
See OM chapter 8.2.2 - Adjustment of bubble detector.
15. Perform a pump calibration by applying MODE FUNCTION - Pump utilities - Calibration.
See OM chapter 5.7 - Pump calibration.
16. Switch off Photometer 5010. Change the cuvette adaptor. Switch on Photometer 5010
17. Do a dark level adjustment by applying utility U1 in combination with standard cuvette adapter.
18. Control the settings by applying utility U12.
See OM chapter 8.2.12 - Printing device configuration.









4.42 DISPLAY UNIT PCB RR-114 ID 5010-009
The display unit pcb connects following components:
• LCD with 2 lines and 16 characters

• Panel with 20 keys
• Sipping lever
• Mother board pcb RR-115
Location
Basis plate
Replacement
Disconnect all plugs from the pcb. The plugs are numerated with J1 up to J5.
Remove four screws fixing the pcb with top case.
Remove one screw fixing earth ground.
Installation
See also exploded view, chapter 5.1 Mantling / Dismantling



4.43 CUVETTE CONNECTION PCB RR-103-V4 ID 5010-019
The cuvette connection pcb connects:
• Mother board pcb RR-115

• Flow through cuvette adaptor or standard cuvette adaptor
• Heating unit
• Bubble detector for sip control
Location
Optical unit
Replacement
Remove 4 screws of cuvette adaptor well (incl. heating unit) and 2 screws between cuvette adaptor well and
heating unit.
Unscrew board and disconnect plug.
Installation

4.43 CUVETTE CONNECTION PCB RR-103-V4 ID 5010-019

4.44 PRINTER CONTROLLER PCB ID 5010-020
The printer controller pcb is a microprocessor subsystem. The printer mechanism is driven by the printer
controller pcb.
Location
Top case
Replacement
Unplug conectors CN1, CN2 and CN4.
Remove four screws fixing pcb.
Installation
All switches of SW1 have to be set to OFF.
In case of using the spare part: printer controller pcb ID 1707418001

The solder strap B14 has to opened and the solder strap B16 has to closed.
18 pin
1
CN1
1
CN6
8
CN4
26 pin
SW1 1
CN5
CN2
1
1
solder strap B14
solder strap B16 6 pin

4.50 FAN ID 1706985001
The fan has a nominal voltage of 12 VDC.
The fans blows the air out of the system for inside cooling.
Location
Backside of Photometer 5010, basis plate.
The plug P3 ist connected with the plug ST3 on the mother board pcb RR-115.
Replacement
Unplug P3 from the mother board pcb RR-115.
Remove the four screws (A).
Clean air vents.
Installation
bl rd
2 1 P3

4.51 BOTTOM CASE ID 1706861001
The bottom case is one of the two main parts of the housing.
Location
Bottom of Photometer 5010
Replacement
The groundplane has to be separated from the bottom case. The groundplane is fixed by six srews with the
bottom case.
Remove the bottom case from the groundplane.
Installation

4.52 CARTON PHOTOMETER 5010 ID 1706896001
1 Aspirate tubing
1 Axis printer paper ID 1706853001
Paper 1 Dust cover ID 1704818001
Paper 2 Fuses for line power
1 Mains cable ID 0552402001
2 Printer paper
1 Pump tube with joints ID 5010-013
1 Ribbon band printer ID 1010735001
1 Standard cuvette adaptor ID 1704800001
1 Top cover small for printer ID 1707574001
1 Waste tubing
Plastic foil
Photometer 5010
BOEHRINGER
MANNHEIM

5 MANTLING / DISMANTLING
display glass
top case ID 1706934001
ID 5010-005 top cover small for printer
ID 1707574001
display with controller pcb

Metal tube inlet ID 5010-009
ID 1703230001
keyboard
ID 5010-002
microswitch with plug

ID 1707248001
lamp shield
bottom case
ID 1706861001
4 x rubber foot
ID 1707469001

mains power circuit fan mother pcb RR-115

ID 1706985001 ID 5010-115
see page see page

5.3 5.5

pump tube with joints

ID 5010-013
bubble detector with plug

ID 1706870001
connector bubbledetector
ID 1706900001
pump motor with plug

ID 5010-017
see page
5.4

photodiode with plug

ID 1707388001
pcb cuvette connection

ID 5010-019
transistor TIP 132 with plug

ID 1707612001
Peltier element with plug

ID 1707370001

5
filterwheel with filters toothbelt for filterwheel motor step with plug
ID 1707051001 ID 1707540001 ID 1707540001
RIELE BERLIN
snapring for filterwheel
ID 1707485001
halogen lamp 12V / 20W lampholder

ID 0554871001 ID 1707205001
Photometer 5010 / V4
MANTLING / DISMANTLING
light barrier with plug

ID 1707213001
5.5
09.09.2003
6.1 COMMUNICATION PROTOCOL
The settings of the serial interface are as follows:
Type of interface: RS 232 C

Baudrate: 9600 bit/s or 4800 bit/s
Data format: 7 data bits, 1 stop bit
Parity: even
Protocol: STX - [string] - ETX – BCC
( STX = start of text, ETX = end of text, BCC = block code character )
Checksum: The BCC is generated from the data string including the ETX (not STX).
Parity of BCC is even.
Hardware connection: 1:1 data cable with the following pin configuration:
25-pin 9-pin Photometer 5010
9-pin female
3 2 2 RXD (Receive Data)
2 3 3 TXD (Transmit Data)
7 5 5 GND (Signal Ground)
7 RTS (Request to send)
8 CTS (Clear to send)
The procedure of communication is as follows:
First activate the remote mode of the Photometer 5010 starting serial interface utilities (OM chapter 5.8).
The communication starts with a block where the photometer (master) enables the computer (slave) to
send a command (ACK = acknowledge, NAK = not acknowledge):
Photometer 5010 <=> Computer
STX MØ ETX BCC =>
<= ACK (or NAK)
STX C ETX BCC =>
<= ACK (or NAK)
Within the next block the computer gives the command (for the list of commands see next page):
Photometer 5010 <=> Computer
<= STX [command] ETX BCC
ACK (or NAK) =>
optional, if an answer is requested:
STX [answer] ETX BCC =>
<= ACK (or NAK)
The two blocks are appearing like a loop alternately one after the other.
Exit from the loop is possible with the command „Z“.

List of commands:
Command Action Answer
A [xxxxx] Set: Device number

[xxxxx] = value
A Query: Device number A [xxxxx]
e.g.: A3501
B Query: Software version B [version, date of version /
version M1 / version M2]
e.g.:
BV4.0a 03.07.03 D/2.0/1.0
D Set: Dark level adjustment
E [x] Query: Dark values of E [dark values]
standard cuvette adaptor E and e.g.:
flow-through cuvette adaptor D E 111 D 123
[x] = multiplexer level 0...7
F Set: Measuring zero F [value]
e.g.: F 5.2739
F0 Set: Measuring zero with multiplexer F [value]
level select e.g.: F 5.2740
G Set: Result without delay G [Result]
e.g.: G 0.3596
H [x] Set: Multiplexer level
H Query: Multiplexer level H [0], …, H [7]
e.g.: H6
I Query: ADC value I [ADC value]
e.g.: I 174066
J Query: Structure of method memory J [string length of a method;
number of basis methods;
number of programmable
methods;
number of pre-programmed
methods;
total number of methods]
e.g.: J 36; 19;230; 1;250;
K [method no.] Query: Method string K [method no.;
method status;
calculation procedure /
temperature level;
wavelength;
no. of standards;
factor / standard;
volume
reserved
method name
unit
time 0
time 1
limit low
limit high
adress of parameters]
e.g.:
K 1; 19; 1; 0; 0; 0; 0;0;
C/F; ; 0; 1; 0; 0;
0;

L [method no.; Set: Method string

method status;
calculation
procedure /
temperature
level;
wavelength;
no. of
standards;
factor /
standard;
volume
reserved
method name
unit
time 0
time 1
limit low
limit high
adress of
parameters]
N Query: Show actual position x of filter N [1], …, N [9]
wheel e.g.: N1
N [1], …, N [9] Set: Turn to position of filter wheel
O [xxx] Set: Wash: xxx µl
P [xxx] Set: Pump with bubble detector In case of a detected bubble
the error code is C 45 instead
of C.
Q Query: Language Q1 or Q2
Q1 Set: English language
Q2 Set: German language
R Set: Reset
S Query: Printer ON / OFF S [0], …, S [3]
e.g.: S1
S1 Set: Printer internal ON
S2 Set: Printer external ON
S3 Set: Printer internal and external ON
S0 Set: All printers OFF
T Set: Clear all programmed methods
U Query: Temperature U [0], …, U [3]
e.g.: U0
U0 Set: Temperature control OFF
U1 Set: Temperature control = 25 °C

V1 Query: Temperature value 25 °C and V [xxxxx]

standard cuvette adaptor e.g.: V11570
V1 [xxxxx] Set: Temperature value 25 °C and
standard cuvette adaptor
flow-through cuvette adaptor e.g.: V10015
V4 [xxxxx] Set: Temperature value 25 °C and flow-
through cuvette adaptor
through cuvette adaptor
through adaptor
W Query: Required value of temperature W [xxxxx]
control e.g.: W 0
X Query: Actual value of temperature X [xxxxx]
control e.g.: X14102
Y Query: Stability of temperature control Y0 or Y1
Z Set: Suspend remote control After a delay of 5 seconds
the system sends a protocol
„M0“ in distances of
4 seconds until an „ACK“ is
received by an external
device.
a [xxxx] Set: Calibration of temperature
b Query: Beep ON / OFF b0 or b1
b0 Set: Beep OFF
b1 Set: Beep ON
c Reset system status
d Query: ADC of temperature control d [xxxxx]
e.g.: d 940
e Query: Type of cuvette adaptor e1 or e2
e1 = Standard cuvette adaptor
e2 = Flow-through cuvette adaptor
f [x] Query: Settings of filter positions [x] f [x] [www]
[www] = value of wavelength e.g.: f3 546
f [x] [www] Set: Value of wavelength of filter position
g Set: Acoustic signal
h not used

i [x] Set: Character of PCB layout version

[x] = character e.g.: a; b; c; d; ...
i Query: Character of PCB layout version i [x]
e.g.: ia; ib; ic; id; ie; ...
j [cccccccccccccccc] Set: Laboratory name
[cccccccccccccccc] = maximum 16 characters
j Query: Laboratory name j [cccccccccccccccc]
e.g.: j Lab 1
k [x] Set: User name
[cccccccccccccccc] [x] = Number of user in the range 1 up to 5
[cccccccccccccccc] = maximum 16 characters
k [x] Query: User name k [x] [cccccccccccccccc]
[x] = Number of user in the range 1 up to 5 e.g.: k3 Tom

6.2 EXCHANGE OF EPROM
The software of the Photometers 5010 is structured as follows:

• operating system
• method data
• text data
The program can be distributed in an EPROM IC14 (27C512). The EPROM is marked with a label e.g.:
P100 V4.xa
dd.mm.yyyy
Replacement of EPROM
1. Before start of replacement empty tube system absolutely by [WASH] and the wash lever.
2. Switch off the device and disconnect the mains cord.
3. Turn device on its top side.
4. Dismantle lid of EPROM.
5. The EPROM become visible as shown below:
P100 V4.xa
dd.mm.yyyy
6. Replace the EPROM by using an IC extractor. Notice that electrostatic discharge can destroy the
EPROM and the motherboard of Photometer 5010. Avoid to produce shortcuts on the pcb. Some
pins and connections are connected to the power supply (battery) of the RAM.
7. Close lid.
8. Turn device on its bottom side.
9. Connect device with the mains and switch it on.

6.3 DOS: DOWNLOAD TO FLASH MEMORY
FLASH Memory
The FLASH memory IC14 (28F512) is a non-volatile memory which may be erased and programmed
electrically. This feature allows to replace the software of Photometer 5010 consisting of:
• operating system
• method data
• text data
The process is done by a serial interface utility DOWNLOAD ( see OM chapter 5.8) and a software modul
running on a personal computer. The devices Photometer 5010 and the personal computer have to be
connected by a serial interface cable.
1. Connect the devices Photometer 5010 and the personal computer by a serial interface cable.
2. Switch on both devices.
3. Install in a new directory of the personal computer the files DL_5010.EXE, D_LOAD.HEX and
P_100.HEX.
4. Photometer 5010: Start the serial interface utility DOWNLOAD ( see OM chapter 5.8) by [MODE]
[SERIAL] [5] [ENTER].
5. Personal computer: Change to DOS-input level. Select the directory with the installed three files.
Start the program file DL_5010.EXE.
DL_5010.EXE is a stand alone executable file for IBM/DOS systems.
6. The menu shows following items:
0 -> END of application

1 -> Select port COM 1
2 -> Select port COM 2
3 -> DOWNLOAD to Photometer 5010
4 -> RESUME after malfunction
7. By item 1 or item 2 the port of the serial interface can be selected. After starting the program the
default value is COM 1.
8. By item 3 the DOWNLOAD to Photometer 5010 is startet.
A progress bar shows the transferring of the files D_LOAD.HEX and P_100.HEX to Photometer
5010. The operation needs a time of approximate three minutes.
IMPORTANT: Be sure that during this operation there is no power fail. Do not interrupt the running
program.
9. After end of operation Photometer 5010 performs a restart operation and shows the start menue.
Otherwise in case of malfunction:
10. The Photometer 5010 shows a dialog after finishing the DOWNLOAD to Photometer 5010 and after
waiting more than five minutes:
DOWNLOAD Vx.x
PLEASE WAIT
11. Switch off Photometer 5010.

13. Dismantle lid of FLASH MEMORY or EPROM (see drawing in SERVICE MANUAL
chapter 6.2).

6.3 DOS: DOWNLOAD TO FLASH MEMORY
14. Change the position of the jumper beside the FLASH memory IC 14 (29F010).
Jumper position: Bootstrap
FLASH memory
29F010

17. If the Photometer 5010 shows the dialog:
Personal computer: Start the program file DL_5010.EXE.
DOWNLOAD Vx.x Select item 4 -> RESUME after malfunction.
PLEASE WAIT The file P_100.HEX will be loaded. A progress bar shows the transferring of
the file. The operation needs a time of approximate three minutes.
IMPORTANT: Be sure that during this operation there is no power fail. Do not
interrupt the running program.
After end of operation the PC application is closed.

19. Change the position of the jumper beside the FLASH memory IC 14 (29F010).
Jumper position: Normal
FLASH memory
29F010
20. Switch on Photometer 5010. The display shows the start menue.

6.4 WINDOWS: RESUME AFTER MALFUNCTION
In case of having a malfunction during download of operating system the Photometer 5010 shows following
dialog after finishing the download to Photometer 5010 and after waiting more than 10 minutes:
DOWNLOAD Vx.x
PLEASE WAIT
Following procedure is recommended:

3. Dismantle lid of FLASH MEMORY or EPROM.
Jumper position
Normal
FLASH MEMORY
Bootstrap
Jumper
4. Change the position of the jumper beside the FLASH memory IC 14 (29F010) to bootstrap.
7. Run on PC the application „Serial Monitor 5010“ (Smon5010.exe)“.

8. Select „Download operating system“.
9. Select the second option – Resume after malfunction – and confirm with [ENTER].
10.
An open dialog is shown. Open the file e.g. P_100.hex. Never open the file D_load.hex. The
download begins…
The window is closed and the progressbar shows the proceding of operation. After three up to
seven minutes the PC stops the transferring of data.
11. After end of operation switch off Photometer 5010.
12. Change the position of the jumper beside the FLASH memory IC 14 (29F010) to normal.
13. Close lid


15. After updating the general operating software of Photometer 5010 several initialization
procedures have to be done by using service tools and utilities.
A detailed description of the procedure is written in the Operating Instruction of Serial Monitor
5010 chapter 4.5.

6.5 INITIALIZATION OF BOOTSTRAP
If the display of Photometer 5010 shows black rectangles in the first line after switching on the instrument,
the bootstrap is destroyed. The bootstrap has to be initialized by using an EPROM containing a valid
operating system.
Following procedure is recommended:

1. Switch off Photometer 5010 and disconnect the mains cord.
2. Be sure working with flow-through system.
4. Dismantle lid of FLASH MEMORY or EPROM.
Jumper position
Normal
FLASH MEMORY
Bootstrap
Jumper
5. Replace the FLASH MEMORY by an EPROM containing a valid operating system (see Service
Manual chapter 6.2). Notice that electrostatic discharge can destroy the FLASH MEMORY or
EPROM and the motherboard of Photometer 5010. Avoid to produce shortcuts on the pcb.
Some pins and connections are connected to the power supply (battery) of the RAM.
6. The position of the jumper beside the FLASH MEMORY IC 14 (29F010) has to be normal.
8. Connect PC and Photometer 5010 with a data interface cable.
9. Connect Photometer 5010 with the mains and switch it on.

10. When errors appear during operation, first of all they have to be confirmed with [ENTER] before
remedying (see chapter OM chapter 10 - ERROR MESSAGE / CORRECTION).
11. Date and time can be set (see OM chapter 8.2.13 - Real time clock ON / OFF):
DATE mm.dd.yy
TIME hh:mm:ss

METHOD NO.
INPUT 1-250
13. Start DOWNLOAD by [MODE], [SERIAL], [5] and [ENTER].

DOWNLOAD
15. Run on PC the application „Serial Monitor 5010“ (Smon5010.exe)“.

See chapter OM Serial Monitor 5010 chapter 4.5.
16. During proceding of operation a warning box is shown on the screen of the PC:
ERROR 1: Timeout
Press [Ok].
17. Close window by the [CLOSE BUTTON].
An error message is shown:
Error Code: 19 [ VARIABLE NOT INITIALIZED ]
Ignore the error message by pressing button [Abort] and finish the application.
DOWNLOAD Vx.x
PLEASE WAIT
19. Switch off Photometer 5010 and disconnect the mains cord.
20. Replace the EPROM by the FLASH MEMORY.
21. The position of the jumper beside the FLASH MEMORY IC 14 (29F010) has to be Bootstrap.
22. Connect Photometer 5010 with the mains and switch it on.
23. Continue with chapter 6.4 - Resume after malfunction.
Perform item 7 up to 13.
24. After loading the general operating software of Photometer 5010 an initialization can be done by
using a special keycode. The procedure makes a default setting of several variables.
Attention: The performing of the following instructions may only be done in case of having a
cleared RAM or a replaced battery.
Description of keycode:
Press and hold the button [WASH], switch on the instrument and wait until a beep.
Let the button off.
Press within the next two seconds the button [ZERO] and wait until a beep.
Let the button off.

25. Date and time can be set (see OM chapter 8.2.13 - Real time clock ON / OFF):
DATE mm.dd.yy
TIME hh:mm:ss

26. Photometer 5010 performs a reset operation. The display shows an error:
PHOTOMETER 5010
ERROR 8
Confirm the error with [ENTER].

METHOD NO.
INPUT 1-250
28. The serial number can be set by applying the service tool S4.
See Service Manual chapter 3.4 - S4 Serial number.
29. The assignment of filter positions has to be done if there are additional filters build in
(e.g. 623 nm). Apply the tool S6.
See Service Manual chapter 3.4 - S6 Filter select.
30. Make a setting of temperature values by applying the service tool S8.2 / S8.3.
The values are noted on the instrument card.
Besides the values are noted on a sticker.
See Service Manual chapter 4.40 - Thermal adjustment.
See Service Manual chapter 3.4 - S8.2 Temperature value of standard cuvette adaptor.
See Service Manual chapter 3.4 - S8.3 Temperature value of flowthrough cuvette adaptor.
31. Make a setting of “attribut of PCB layout“ by applying the service tool S13:
Software applications may be dependent on this attribut.
See Service Manual chapter 3.4 - S13 Attribut of PCB layout.
32. Make a dark level adjustment by applying utility U1 in combination with flow-through system.
The utility is working specific to the current adaptor. An adjustment of the standard cuvette
adaptor should be done later on.
33. Make a media test by applying utility U2.
See OM chapter 8.2.2 - Adjustment of bubble detector.
34. Perform a pump calibration by applying MODE FUNCTION - Pump utilities - Calibration.
See OM chapter 5.7 - Pump calibration.
35. Switch off Photometer 5010. Change the cuvette adaptor. Switch on Photometer 5010
36. Make a dark level adjustment by applying utility U1 in combination with standard cuvette
adapter.
37. Control the settings by applying utility U12.
See OM chapter 8.2.12 - Printing device configuration.

7 TROUBLE SHOOTING
CODED ERROR MESSAGES

Legend : SM = Service Manual / OM = Operator’s Manual
No. (possible) Causes Remedy
1 - not occupied -
2 check sum of a free programmed method is wrong program new method;
check voltage of battery (see SM 4.40.3)
3 forbidden input, wrong number format repeat input in permitted area
4 method not available Method editor:
check method no.
5 dark value is absolutely too high (> 16 bit) or higher as repeat dark level adjustment;
the measurement check lamp / filter;
ADC overflow check blank;
adjust amplifier stage (see SM 4.40.6)
6 all multiplexer positions are too bright/too dark at setting repeat dark level adjustment;
to zero check filter / lamp;
check blank;
7 mathematical overflow, at measurement calculation check filter; check standard;
check measuring solution
8 check sum error in the data record of the dark offset repeat dark level adjustment;
check voltage of battery (see SM 4.40.3)
9 check sum error in the data record of the device automatic error remedy;
standard adjustment (status, ADC correction) check voltage of battery (see SM 4.40.3)
10 division by a too small value (< 0.0156 Bel) check filter; check standard; check measuring solution
11 - not occupied -
12 setting to zero not possible (zero value is < 32768 cycle) check lamp;
check filter;
check zero solution;
13 setting to zero not possible (zero value is > 983039 check lamp;
cycle) check filter;
check zero solution;
14 no valid standard measure valid standard solution
15 no parameter memory vacant (too little memory for delete a no longer actual nonlinear method
nonlinear methods)
16 method no. is occupied select other method no.; delete a no longer actual
nonlinear method
17 check sum error in the parameter memory (nonlinear program method newly;
method) check voltage of battery (see SM 4.40.3)
18 at calculation overflows in nonlinear method check factor; check parameter
19 clock malfunction
20 overflow at Kinetic check measuring solution

7 TROUBLE SHOOTING

29 Input of deltas or time per delta wrong restart method
30 - not occupied -
31 communication: wrong data format check data sent at the communication partner formally
32 communication: sent data not plausible / not check data sent at the communication partner as
interpretable regards content
33 communication: mentioned module does not answer in check interconnecting cable; check mentioned module
a certain time
34 communication: overflow send/receive buffer reduce amount of data at the communication partner
35 Remote control: wrong method number external software problem
36 Remote control: unknown command external software problem
37 Remote control: wrong data format external software problem
38 timeout at reception from modul 1 switch off/on device
39 check sum error at reception from modul 1 switch off/on device
40 timeout at reception from modul 2 switch off/on device
41 check sum error at reception from modul 2 switch off/on device
42 NAK at reception from modul 2 switch off/on device
43 NAK at reception from modul 1 switch off/on device
44 error at pump calibration clamp pump tube;
control volume of aspiration;
the pump tube is not clamped; control connectors of tubes;
no liquid was aspirated; the plug of the bubble detector has a bad contact;
to much liquid was aspirated when calibrating; the cable of the bubble detector has a bad contact;
the power of the pump is to small; adjust bubble detector (OM chapter 8.2.2);
the motor does not rotate or stops; replace inlet tube (OM chapter 9.7)
connectors of tubes leak;
bubble detector works incorrect;
the inlet tube is blocked;
the inlet tube has a change in color
45 error at sipping solution clamp pump tube;
control volume of aspiration;
the pump tube is not clamped; control connectors of tubes;
no liquid was aspirated; do a calibration of peristaltic pump (OM chapter 5.7);
the power of the pump is to small; the plug of the bubble detector has a bad contact;
the motor does not rotate or stops; the cable of the bubble detector has a bad contact;
connectors of tubes leak; adjust bubble detector (OM chapter 8.2.2);
bubble detector works incorrect; replace inlet tube (OM chapter 9.7)
the inlet tube is blocked;
the inlet tube has a change in color
46 filter position outside tolerance
47 + 12 VDC of halogenlamp out of range check voltage (SM chapter 4.40)

7 TROUBLE SHOOTING
48 + 15 VDC out of range check voltage (SM chapter 4.3)

49 + 5 VDC out of range check voltage (SM chapter 4.40)
50 +/- 12 VDC out of range check voltage (SM chapter 4.40)
51 - not occupied -
52 timeout printer internal The internal printer is temporarily disconnected
53 - not occupied -
54 non-linear measurement: value out of range Control the absorbance of the sample

8 SPARE PARTS
ID-No. Description
0369616001 Interference filter 623nm

0554871001 Halogen lamp 12V/20W
0745235001 Printer ribbon, 5 pcs
1010735001 Printer ribbon, 1 pc
1223003001 Printer mechanism w. cable
1268457001 Printer paper, 5 rolls
1704796001 Flow-through cuvette adaptor
1704800001 Standard cuvette adaptor
1704818001 Dust cover
1704834001 Waste tubing, 2 pcs
1706853001 Axis printer paper
1706861001 Bottom housing
1706870001 Bubble detector with plug
1706896001 Carton Photometer 5010
1706900001 Connector bubble detector
1706926001 Cuvette lense kit
1706934001 Display glass
1706985001 Fan
1707078001 Flatcable analog/digital/printer
1707159001 Holding spring for disp. cuvette
1707167001 Inlet tube peristaltic pump, 5 pcs
1707175001 Joint inlet tube cuvette
1707205001 Lampholder
1707213001 Lightbarrier w. plug filterwheel
1707230001 Metal tube inlet
1707248001 Microswitch with plug
1707370001 Peltier element with plug
1707388001 Photodiode with plug
1707442001 RTC / RAM
1707469001 Rubber foot, 4 pcs
1707477001 Snapring for filter, 5 pcs
1707485001 Snapring for filterwheel

8 SPARE PARTS
ID-No. Description
1707493001 Snapring SB12 / lense

1707523001 Snapring SB21 / heatcut filter
1707540001 Motor stepp w.plug filterwheel
1707558001 Toothbelt for filterwheel
1707566001 Top case
1707574001 Top cover small for printer
1707612001 Heating unit, Transistor TIP132 with plug
1707655001 Printer connection cable
1804553001 Stepmotor driver
501-002 Data cable serial interface
5010-001 Tool kit filter exchange
5010-002 Keyboard self adhesive
5010-004 Filter wheel with filters
5010-005 Operator’s Manual
5010-006 Service Manual
5010-007 EPROM 5010
5010-008 Top case, replacement
5010-009 Display unit PCB RR_114
5010-013 Pump tube with joints
5010-014 Aspirate tubing
5010-015 Outlet tube cuvette, 5 pcs
5010-016 AC/DC power supply
5010-017 Pump motor with plug
5010-018 Fuses for line power, 10 pcs
5010-019 PCB cuvette connection
5010-020 Printer controller
5010-021 Cable AC/DC power supply
5010-022 Fuses T3.15A / 250V, 10 pcs
5010-023 Fuses T6.3A / 250V, 10 pcs
5010-025 Flatcable RR-115 / Display
5010-026 Cable RR-115 / Controller
5010-115 Motherboard RR-115
5010-670 Interference filter 670nm

9.1 CALIBRATION PROCEDURE OF
PHOTOMETER 5010
SUBJECT: CUVETTE TEMPERATURE
1. Purpose
This calibration procedure describes the calibration of the cuvette temperature of Photometer 5010. The
meaning of calibration is to determine the difference between the true temperature and the set
temperature. The procedure is to carry out for the temperatures which are used by the customer.
2. General description and measuring theory

The temperature is measured with a selected thermistor-probe. The thermistor is supplied by RIELE. The
certified tolerance of this thermistor is ± 0.1 °C.
The photometer-cuvette temperature is measured in case of using the single cuvette adaptor in an
aluminium-dummy cuvette or in case of using the flow-through cuvette adaptor in the original flow-through
cuvette.
The resistance of the thermistor is measured by a 4½ digit digital multimeter (DMM). The requirements of
the DMM are given in chapter 7. The corresponding temperature is listed in chapter 8.
The implementation of the whole procedure lasts approximately 1.5 hours.
3. Operating conditions
The calibration should be performed under normal operating conditions, i.e.
• Room temperature: 15 °C to 30 °C
• Relative humidity: 40 % to 80 %
• No ventilation or draft
Note that the variation of the room temperature should not exceed ± 2 °C during the calibration process.
4. Traceability of the calibration standards

The traceability to the National Temperature Standard is given by selected thermistor and a calibrated
DMM. The last Calibration Certificate of both, the DMM and the thermistor, must not be older than
12 months. If the 24 month accuracy of the DMM fulfils the requirements in chapter 7, the last DMM
Calibration Certificate can be up to 24 months old.
5. Test procedure
a. In case of using the single cuvette adaptor insert the dummy-cuvette instead of a square cell.
In case of using the flow-through adaptor remove the joint inlet tube cuvette / aspirate tubing and
the outlet tube cuvette.
Pipette 200 µl distilled water in the dummy-cuvette or the flow-through cuvette.
b. Connect the thermistor lead to the two-wire resistance input of the DMM.
c. Insert the thermistor-probe into the hole of the photometer dummy-cuvette or the hole of the outlet
tube cuvette of the flow-through cuvette.
Caution: Handle the delicate probe with care! Pay attention to the correct position of the probe.
Avoid bubbles within the aperture of the flow-through cuvette.
d. Switch on the DMM and Photometer 5010 and wait 15 minutes for warming up.
e. Select the two-wire resistance function and appropriate range on the DMM. The resistance of the
thermistor is in the range of 6 kOhm up to 10 kOhm for the temperature range of 25 °C up to
37 °C. The table of resistance of thermistor ID 090-063 shows the assigned resistance for a given
temperature.
f. Enter the service program level.

PHOTOMETER 5010
For additional information see chapter 3.4 – SERVICE TOOLS.
g. Perform a system initialization by applying service program item 1 – S1 - System initialization.
h. Enter the service program item 8 – S8 Temperature functions.
i. Select the temperature 25 °C by using service program item 1 – S8.1 - Temperature regulation
on / off. Activate the temperature control by selecting ON and 25 °C.
j. Check the temperature control by using service program item 5 – S8.5 - Temperature required /
actual.
As long as temperature is not stabilized, the character 16 of the first line is alternately showing a
“T” or a black rectangle. It needs minimum five minutes until a stabilized temperature level is
reached. Wait 10 minutes furthermore.
k. Read the displayed measurement of DMM in Ohm. Look up in the table of resistance the
appropriate set of values. Calculate by performing a linear interpolation the assigned temperature
value (see chapter 9).
l. Make an automatic temperature calibration by using service program item 4 – S8.4 - Temperature
calibration.
m. Check the temperature control by using service program item 5 – S8.5 - Temperature required /
actual.
As long as temperature is not stabilized, the character 16 of the first line is alternately showing a
“T” or a black rectangle. It needs minimum five minutes until a stabilized temperature level is
reached. Wait 5 minutes furthermore.
n. Read the displayed measurement of DMM in Ohm. Look up in the table of resistance the
appropriate set of values. The temperature value should show the desired value.
o. Read the required value (first value in the second line) and subtract the value 3000. The result is
called temperature value. Note this value in calibration protocol combined with adaptor type and
temperature level.
See for additional information in chapter 4.40 – MOTHER BOARD – Thermal adjustment.
p. Repeat above mentioned steps with the temperature levels 25 °C, 30 °C and 37 °C for both types
of adaptors.
6. Documentation
For documentation use a form containing following table:
Single cuvette adaptor Flow-through adaptor

25 °C Temperature value Temperature value
7. Requirements for the Digital Multimeter (DMM)

The DMM for the resistance-measurements has to fulfil the following requirements:
• 12 month accuracy of resistance measurements

within the 0 – 30 KOhm range: 0.05 % + 2 digits
• Current for resistance measurement: ≤ 15 µA
• Latest Calibration Certificate: not older than 12 months
If the 24 months resistance-accuracy fulfils the above accuracy requirements, the latest
Calibration Certificate can be ≤ 24 months old.

PHOTOMETER 5010
8. Table of resistance – Thermistor ID 090-063
Temperature Resistance Temperature Resistance Temperature Resistance

[° C] [Ohm] [° C] [Ohm] [° C] [Ohm]
23,5 10682 28,5 8590 35,5 6397

23,6 10636 28,6 8553 35,6 6371
23,7 10589 28,7 8517 35,7 6345
23,8 10543 28,8 8480 35,8 6319
23,9 10496 28,9 8443 35,9 6293
24,0 10450 29,0 8407 36,0 6267
24,1 10404 29,1 8371 36,1 6241
24,2 10358 29,2 8335 36,2 6216
24,3 10313 29,3 8300 36,3 6191
24,4 10268 29,4 8264 36,4 6166
24,5 10223 29,5 8230 36,5 6141
24,6 10178 29,6 8195 36,6 6116
24,7 10133 29,7 8161 36,7 6091
24,8 10089 29,8 8126 36,8 6066
24,9 10044 29,9 8092 36,9 6042
25,0 10000 30,0 8057 37,0 6017
25,1 9956 30,1 8023 37,1 5993
25,2 9913 30,2 7989 37,2 5968
25,3 9870 30,3 7955 37,3 5944
25,4 9827 30,4 7922 37,4 5920
25,5 9784 30,5 7888 37,5 5896
25,6 9742 30,6 7855 37,6 5872
25,7 9700 30,7 7822 37,7 5848
25,8 9657 30,8 7789 37,8 5824
25,9 9615 30,9 7756 37,9 5801
26,0 9573 31,0 7723 38,0 5777
26,1 9531 31,1 7690 38,1 5754
26,2 9490 31,2 7658 38,2 5730
26,3 9449 31,3 7626 38,3 5710
26,4 9408 31,4 7593 38,4 5684
26,5 9368 31,5 7562 38,5 5661

PHOTOMETER 5010
9. Linear interpolation
This procedure describes the performing of a linear interpolation in case of a given resistance R.
The assigned temperature is called T.
If the resistance R is found in the table, get the appropriate temperature value and finish this chapter.
If the actual temperature of the thermistor is in the range of the required temperature ± 1.5 °C, two values
of resistance can be read from table with the relation:
R0 > R > R1
The resistances R0 and R1 form an interval. They are a set of data points next to the resistance R.
The assigned temperature values of the resistances R0 and R1 are called T0 and T1.
The assigned temperature T can be calculated by the formula:
T = T0 + (T1 – T0) × (R - R0) / (R1 – R0)
Example:
In case of a given resistance R with the value 9978 Ohm you can find the interpolated temperature T by:
T = 25 °C + 0.1 °C × (9978 - 10000) / (9956 – 10000)
T = 25.05 °C

SM - V4.1

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Photometer 5010

1 General notes Sm_V4_a.pdf

2 Service concepts Sm_V4_a.pdf

3.1 Block diagram 3.1.1 Sm_V4_a.pdf

4.1 Mains power circuit 4.1 Sm_V4_b.pdf

4.10 Optical unit 4.10.1 Sm_V4_b.pdf

4.20 Bubble detector 4.20.1 Sm_V4_b.pdf

RIELE BERLIN Photometer 5010 / V4 1 22.09.2003

4.30 Top case 4.30.1 Sm_V4_c.pdf

4.40 Mother board RR_115 Sm_V4_d.pdf

4.42 Display unit pcb RR-114 4.42.1 Sm_V4_g.pdf

4.50 Fan 4.50.1 Sm_V4_g.pdf

5 Mantling / Dismantling Sm_V4_h.pdf

RIELE BERLIN Photometer 5010 / V4 2 22.09.2003

6.1 Communication protocol 6.1 Sm_V4_h.pdf

7 Trouble shooting 7.1 Sm_V4_h.pdf

8 Spare parts 8.1 Sm_V4_h.pdf

9.1 Calibration procedure of Photometer 5010

RIELE BERLIN Photometer 5010 / V4 3 22.09.2003

In any case, the respective packaging leaflet should be regarded as authoritative.

1.1 Contact Partner

ROBERT RIELE GmbH & Co KG

Phone: ++49 / 30 / 404 40 87

1.2 Proved Security

This instruments meets protection 1 (earth conductor wire).

1.3 Precaution and Hazards

RIELE BERLIN Photometer 5010 / V4 1.1 20.08.2003

Disinfect and wash hands after work is completed.

• shows visible signs of demage

• has been transported under rough conditions

The Photometer 5010 should be used by appropriately qualified persons only.

RIELE BERLIN Photometer 5010 / V4 1.2 20.08.2003

2.1 Service Level

Handling of Replacement of parts

RIELE BERLIN Photometer 5010 / V4 2.1 21.08.2003

Repair of parts marked with "R"

Emergency shipments require additional costs to be charged.

marked with EMERGENCY

RIELE BERLIN Photometer 5010 / V4 2.2 21.08.2003

− Date (problem date)

Note: RA sent via electronic mail to Berlin is valid without a

All returned parts should be individually labelled with the corresponding RA

ROBERT RIELE GmbH & Co KG

RIELE BERLIN Photometer 5010 / V4 2.3 21.08.2003

RIELE BERLIN Photometer 5010 / V4 3.1.1 04.09.2003

Change to the selection level of the mode functions with [MODE].

[MODE] [4] Parallel printers ON / OFF 5.6

[MODE] [5] Multi-standard tools 5.10

[MODE] [6] Pump utilities 5.7

[MODE] [7] Serial interface utilities 5.8

[MODE] [8] Changing sample numerator 5.9

RIELE BERLIN Photometer 5010 Service 3.2.1 22.08.2003

SELECTION OF UTILITY PROGRAMS

Confirm with [MODE].

Identity Utility Program Descript

RIELE BERLIN Photometer 5010 Service 3.2.2 22.08.2003

SELECTION OF SERVICE TOOLS

Program selection: Change to service program selection with [MODE].

Enter password [13467]. Confirm with [ENTER].

Item no. Service tool Description

RIELE BERLIN Photometer 5010 / V4 3.3.1 19.09.2003

DESCRIPTION OF SERVICE TOOLS

S2 Free methods initialization