Program: B.

Pharm Semester : VII

Course : Instrumental Methods of Analysis - Practical Code: BP705P
Teaching Scheme Evaluation Scheme
Term End
Lecture Practical Internal Continuous
Examinations (TEE)
(Hours per (Hours per Credit Assessment (ICA)
(Marks- 35
week) week) (Marks -15)
in Question Paper)
0 4 2 Marks Scaled to 15 Marks Scaled to 35
Prerequisite : Basic knowledge of chemistry and spectroscopic techniques
Scope: This subject deals with the application of instrumental methods in qualitative and
quantitative analysis of drugs. This subject is designed to impart a fundamental
knowledge on the principles and instrumentation of spectroscopic and
chromatographic technique. This also emphasizes on theoretical and practical
knowledge on modern analytical instruments that are used for drug testing.
Objectives
The course is designed to understand separation techniques (Thin layer chromatography,
paper chromatography, column chromatography, gas chromatography, high pressure liquid
chromatography), characterization techniques (NMR, Mass spectroscopy), modern
hyphenated techniques (LC-MS, LC-MS/MS, GC-MS) and analytical method validation.
Outcomes: Upon completion of the course the student shall be able to
 Understand the interaction of matter with electromagnetic radiations and its applications
in drug analysis
 Understand the chromatographic separation and analysis of drugs.
 Perform quantitative & qualitative analysis of drugs using various analytical
instruments.
Detailed Syllabus: ( per session plan )
Unit Description
1 Determination of absorption maxima and effect of solvents on absorption maxima of
organic compounds
2 Estimation of dextrose by colorimetry
3 Estimation of sulfanilamide by colorimetry

4 Simultaneous estimation of ibuprofen and paracetamol by UV spectroscopy

5 Assay of paracetamol by UV- Spectrophotometry

6 Estimation of quinine sulfate by fluorimetry

7 Study of quenching of fluorescence

8 Determination of sodium by flame photometry
9 Determination of potassium by flame photometry
 10 Determination of chlorides and sulphates by nepheloturbidometry

11 Separation of amino acids by paper chromatography

12 Separation of sugars by thin layer chromatography
13 Separation of plant pigments by column chromatography
14 Demonstration experiment on HPLC

15 Demonstration experiment on Gas Chromatography

Text Books:
1. Text book of Pharmaceutical Analysis by Kenneth A. Connors
2. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
3. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
4. Organic Chemistry by I. L. Finar
5. Organic spectroscopy by William Kemp
6. Quantitative Analysis of Drugs by D. C. Garrett
7. Spectrophotometric identification of Organic Compounds by Silverstein
Reference Books:
1. Indian Pharmacopoeia 2014, Volume I, II, III & IV; Government of India, Ministry of Health
and Family Welfare; The Indian Pharmacopoeia Commission, Ghaziabad.
2. National Formulary of India, 2011; Government of India, Ministry of Health and Family
Welfare; Indian Pharmacopoeia Commission.
3. British Pharmacopoeia 2014, Volume 1, 2, 3, 4 & 5; British Pharmacopoeia Commission.
4. USP 37/ NF 32, 2014, Volume 1, 2, 3 & 4; The United States Pharmacopeial Convention,
Rockville, MD.
5. European Pharmacopoeia 2014 8.0; Volume 1 & 2; EDQM Publication.
6. The International Pharmacopoeia; 3rd edition; 1995 Volume 1, 2, 3 & 4; WHO, CBS
Publishers & Distributors.
Any other information :
• http://www.cis.rit.edu/htbooks/nmr/
• http://en.wikipedia.org/wiki/NMR_spectroscopy
• www.astbury.leeds.ac.uk/facil/MStut/mstutorial.htm
• http://teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/chrom1.htm
• http://en.wikipedia.org/wiki/Chromatography
• http://www.rpi.edu/dept/chem-eng/Biotech-Environ/CHROMO/chromintro.html
• http://www.ptfarm.pl/pub/File/Acta_Poloniae/2012/3/371.pdf

Total Marks of Internal Continuous Assessment (ICA) : 15 Marks

Distribution of ICA Marks :

Description of ICA Marks

Sessional Practical Examination 10
Term Work 5
Total Marks : 15

____________ ____________
Signature Signature
(Prepared by) (Approved by)
Dr.Mayur Yergeri Dr.Bala Prabhakar
Dean, SPPSPTM