Professional Documents
Culture Documents
DNA
Technolgoy
Esther Sparks, Bri'an Barrow,
Michaela Palmer, Brianna Walters,
Arielle Johnson
Presentation
Outline
Examples
What is
Recombinant
DNA
Technology?
WHAT IS RECOMBINANT DNA
TECHNOLOGY?
1. Gene Cloning: The isolated genes are cloned, typically into a plasmid vector,
which serves as a carrier for the foreign genes.
2. Transformation of Rice Cells: The cloned genes are introduced into rice plant
cells using a method such as agrobacterium-mediated transformation or
biolistic bombardment (gene gun). This process can be performed in tissue
culture under controlled conditions.
3. Integration into Rice Genome: The introduced genes integrate into the rice
genome, often at random locations. This step is critical for stable inheritance of
the desired trait in subsequent generations of rice plants
4. Regeneration of Transgenic Rice Plants: The transformed rice cells are cultured
to regenerate whole plants. This involves selecting and nurturing cells that have
successfully incorporated the foreign genes and developed into viable plants.
Diagram Showing Golden Rice
Production
RECOMBINANT
VACCINNES
https://www.youtube.com/watch?v=DgDDw2gQJx0
QUESTIONS
https://www.britannica.com/science/recombinant-DNA-
technology/Creating-the-clone (additional information)
https://www.youtube.com/watch?v=8cYvyYOjzOc (information
on DNA isolation)
https://www.vedantu.com/biology/recombinant-dna-
technology (information on the steps for recombinant DNA
technology)
https://www.genscript.com/what-is-restriction-
digestion.html#:~:text=Restriction%20digestion%20is%20acco
mplished%20by,outside%20of%20the%20recognition%20seque
nce. (information on restriction enzymes)
REFERENCE
https://www.evitria.com/journal/recombinant-
antibodies/recombinant-dna-technology/
https://www.nlm.nih.gov/exhibition/fromdnatobeer/exhibition-
interactive/recombinant-DNA/recombinant-dna-technology-
alternative.html#:~:text=Recombinant%20DNA%20is%20a%20te
chnology,insulin%20gene%20in%20the%20laboratory.
(examples)
https://www.vedantu.com/biology/recombinant-dna-
technology (rDNA insertion into host, isolation of recombinant
cells)