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  • Molecular Biology: Investigations Result Unit

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    This document summarizes the results of a COVID-19 PCR test. The test was performed on a 21-year-old male named Mr. Om Shahi. Nasopharyngeal and oropharyngeal swabs were collected and tested using real-time RT-PCR. The test results for both the E gene and Orf1a gene were negative, resulting in an overall negative test result. The test works by amplifying target regions of the virus and detecting them through a threshold cycle. It has a clinical sensitivity and specificity of 100% when validated against a gold standard PCR test.

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    2022030605

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    This document summarizes the results of a COVID-19 PCR test. The test was performed on a 21-year-old male named Mr. Om Shahi. Nasopharyngeal and oropharyngeal swabs were collected and tested using real-time RT-PCR. The test results for both the E gene and Orf1a gene were negative, resulting in an overall negative test result. The test works by amplifying target regions of the virus and detecting them through a threshold cycle. It has a clinical sensitivity and specificity of 100% when validated against a gold standard PCR test.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    4 views1 page

    Molecular Biology: Investigations Result Unit

    Uploaded by

    bzhzprmn77
    This document summarizes the results of a COVID-19 PCR test. The test was performed on a 21-year-old male named Mr. Om Shahi. Nasopharyngeal and oropharyngeal swabs were collected and tested using real-time RT-PCR. The test results for both the E gene and Orf1a gene were negative, resulting in an overall negative test result. The test works by amplifying target regions of the virus and detecting them through a threshold cycle. It has a clinical sensitivity and specificity of 100% when validated against a gold standard PCR test.

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    © All Rights Reserved
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    of 1

    UHID NUMBER : 2022030605 REG.

    DATE : 06-03-2022 10:04 AM

    PATIENT NAME : Mr. OM SHAHI COLLECTION DATE : 06-03-2022 10:04 AM

    AGE/ GENDER : 21 Year/Male REPORTING DATE : 06-03-2022 02:46:22 PM

    SERIAL NO. : 21432

    REFFERED BY : SELF

    SPECIMEN : NASOPHARYNGEAL&OROPHARYNGEAL SWABS CASE ID : GORN0076359034

    Investigations Result Unit Biological Reference Range

    MOLECULAR BIOLOGY
    COVID 19 REAL TIME RT-PCR
    CoV-19 E Gene NOT DETECTED
    CoV-19 Orfla Gene NOT DETECTED
    FINAL RESULT NEGATIVE

    ICMR APPROVED LABCODE : MAMPAGOUP

    Technology: tests works on principle of Real Time Reverse Transcription Polymerase Chain Reaction (real time RTPCR).
    It involves the specific amplification of target region. This analysis is done on Truelab real time PCR by using the
    sensitive and specific Taqman assay method. Amplified products are indicated by threshold cycle (Ct) in amplification
    curve.
    Analytical Sensitivity: The test results were found to 100% concordant with the results of the Gold standard (Real Time
    PCR used at State level VRDL , BMCRI, Bengaluru). The clinical sensitivity, Clinical specificity and overall concordance
    were determined to be 100%. The POC test herewith is recommended for Confirmation of SARC COV 2 Cases.The
    Performance of this test is been Validated and Evaluated by ICMR.
    Target Selection:The target sequence used for COVID-19 is the Eand Orf1a genes. Human RNaseP gene serves as a full
    process internal control (IPC) for proper swab collection, nucleic acid extraction and PCR.
    Method:- Real Time PCR.

    Note: Assay result should not be interpreted only in the context of other laboratory findings and the total clinical status of
    patient

    --------------End of Report--------------

    2022030605

    DR.MASTRAJ SINGH
    MD PATH

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