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Initially, crude methanol extract was tested for its ability to inhibit extracellular promastigotes of L.
The discovery of antibiotics to combat these pathogens marked a resolution in the 20th century. The
aim of the present study was to evaluate the in vitro antioxidant activities of methanolic extract of
Lawsonia inermis L. Keywords: Flavonoids; Henn a; Pharmacological act ion; Phenolic c ompounds.
This urged us to implement new bioanalytical technologies for faster analyses targeting the bioactive
constituents. XRD analysis exhibited the crystalline nature of AgNPs and showed face-centred cubic
structure. Article types Author guidelines Editor guidelines Publishing fees Submission checklist
Contact editorial office Submit your research Search Download article. The research scholarship to
KI is provided by the International Research Support Initiative Program by the Higher Education
Commission of Pakistan. Antimicrobial and Antioxidant Potentials of Selected Yemeni Medicinal
Plants from the Island Soqotra. The synthesised AgNPs revealed significant antibacterial activity
against gram-positive bacteria. The characteristics of the synthesized silver nanoparticles suggest their
application as a potential antimicrobial and anticancer agent. While both luteolin ( 5 ) and apigenin (
6 ) have previously been shown to possess activity against L. Other group of molecules reported
from this plant include quinones, phenylpropanoids, flavonoids, terpenoids, phenolics, fatty acids,
carbohydrates, proteins, tannins, alkaloids, xanthones, coumarin, glucosides, naphthoquinone,
saponins, triterpenoids, sterols and dioxin derivatives. Stability assay: The Butantan surfactant used
in this assay had been stored for one year in the refrigerator (4 to 8?C) and its stability was
evaluated in distinct assay conditions using a premature rabbit model. Furthermore, the double bond
between C-2 and C-3 proved to be important for the activity. MRSA in the community: Summary of
an experts’ meeting convened by the Centers for Disease Control. Activities of Essential Oils from
Leaves and Fruits of Commiphora caudata Engl., Int. J. of. This plant contain other chemicals such
as isoplumbagin, apigenin, apigenin glycosides, luteolin, luteolin-7 glucosides, p-coumarin and
lupeol among which many are reported for their cytotoxicity and chemopreventive activity against
different type of cancer cell. Pharmacognosy and Phytochemical Research, 7(1) (2015) 38-44.
Interrupted mitochondrial membrane potential, release of cytochrome C, activation of caspases 3 and
9 fetched apoptosis in Ca9-22 cells via inactivation of the EGF receptor Chien et al., 2010. Amino-
derivatives of lawsone and lapachol were found to be cytotoxic against Ehrlich carcinoma and
human K562 (leukemia cells). The tumour inhibitory tendency was sustained during the 20-week test
period. Preliminary observations were recorded in terms of colour change of the reaction mixture
from yellow to greyish black. In vitro antiplasmodial activity was evaluated on chloroquine sensitive
NF-54 strain by the determination of pfLDH a. Wash fastness Dry rubbing Wet rubbing Light
fastness. Masters’ dissertation, Kent State University, Kent, OH. The plant has been reported to have
analgesic, hypogl y ce mic, hepatoprotecti ve, immunostimulant, anti-inflammat ory, antibacterial,
antimicrobial, antifungal, antiviral, antiparasitic, antitr ypanosomal, antidermatoph ytic, antioxidant,
antifertility, tubercul ostatic and anticancer propertie s. Today, it is ballparked that a propos of 70-95
% people dwelling in developing nation relies on traditional medicines for their primary health care
Dahanukar et al., 2000. Drugs from plant origin posturing to become source for medicine have led to
an unexpected augmentation in the number of herbal industries Kirtikar and Basu, 2005. The
dynamic light scattering studies have revealed an average size and surface Zeta potential of the NPs
as, ?39.5 nm and ?21.6 mV, respectively. The potential antibacterial and antifungal activities of the
AgNPs were evaluated against Bacillus subtilis, Staphylococcus aureus, Candida kruseii and
Trichophyton mentagrophytes. You can download the paper by clicking the button above. Results:
No specific antibodies were detected to P.
To link to this page, Copy and Paste the following HTML code. The characterization of AgNPs were
analyzed using UV visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy
(TEM) and Fourier transform infra-red spectroscopy (FTIR). The studied methanol and water leavse
extracts of L. This review gives a view mainly on the traditional uses, phytochemistry and
pharmacological actions of the plant. Initially, crude methanol extract was tested for its ability to
inhibit extracellular promastigotes of L. We have included maximum available information from its
in vitro and in vivo studies by referring different websites, text books, notes, and articles, abstract,
summary and consulting worldwide accepted scientific databases. The extract was subjected to
phytochemical screening using standard procedures, and the antimicrobial activity against three (3)
bacterial isolates (Escherichia coli, Staphylococcus aureus and Pseudomonas aerugenosa) and two (2)
fungal isolates, (Candida Albicans and Microsporum canis.) were tested using Kirby bauer disc
diffusion method. Hence, the isolated bacterial isolates were confirmed asS.aureus, S.mutans,
P.aeruginosa and fungal isolates such as A.niger, A.flavus and Fusarium. In this study, antimicrobial
activity was performed by disc diffusion and well diffusion method. The present study was
conducted to evaluate the antimicrobial activity from ethanolic extract against different life
threatening pathogenic microorganisms and screening for various phytochemical constituents of
Lawsonia inermis Linn. Phytochemical component in the extract that possess antibacterial properties
such as saponin, tannin, alkaloid, flavonoid, steroid and phenolic were also determined. Each of the
increments constituted 500 mL solvent, which yielded 89 fractions. A solution of sodium formate
clusters was injected in the beginning of each run to enable internal mass calibration. It is now
considered as a valuable source of unique natural products for development of medicines against
various diseases and also for the development of industrial products. About 1500 g of the powder
was extracted using Soxhlet apparatus for 12 h using 5.0 L of methanol as solvent. Download Free
PDF View PDF Current Issues in Pharmacy and Medical Sciences Phytochemical and antioxidant
activities of methanolic extract of Lawsonia inermis L. In this ethanol extraction also shows
maximum activity (100%). This work is licensed under a Creative Commons Attribution 4.0
International License. Pseudomonas aeruginosa JP2 isolated from metal contaminated soil was
evaluated towards extracellular synthesis of silver NPs (AgNPs). Download Free PDF View PDF
Plant-mediated green synthesis of silver nanoparticles using trifolium resupinatum seed exudate and
their antifungal efficacy on neofusicocum parvum and rhizoctonia solani Samira Salari, M K, Pooya
Ghasemi Nejad Almani In recent years, biosynthesis and the utilization of silver nanoparticles
(AgNPs) has become an interesting subject. Dogs are considered as the main reservoirs of visceral
leishmaniasis (VL) in the urban environment and diagnosis improvement was fundamental in this
species. Leaves are small, opposite in arrangeme nt along the branches, sub-sessile, about 1.5 to 5 cm
long, 0.5 to 2 cm wide, greenish brown to dull g reen, elliptic to broadly lanceolate with entire
margin, petiole short and glabrous an d acute or obtuse apex with tapering base. Download Free PDF
View PDF See Full PDF Download PDF Loading Preview Sorry, preview is currently unavailable.
Council of Scientific and Industrial Research New Delhi, 1962, 6, pp. Add 'Search on Flickriver' to
your browser's search box. It is worthy to notice that lawsone itself has significant antioxidant and
anti-inflammatory activity that could be major characteristic of numerous anticancer phytochemicals.
IHC showed sensitivity of 98.51 %, specificity of 1. Allyl-amine derivatives of lawsone and lapachol
were found potent cytotoxic with an IC 50 values of 23.89 and 16.94 ?M respectively da Silva et al.,
2002. Dichloroallyl lawsone, an analog of the lapachol, and acivicin inhibit the biosynthesis of
nucleotide and showed anticancer activity against certain experimental tumor models Kemp et al.,
1986. Dichloroallyl lawsone is an important chemotherapeutic agent causes cardiac toxicity in the
rhesus monkey McKelvey et al., 1979. Further, the size, charge, and polydispersity nature of AgNPs
were studied using dynamic light scattering spectroscopy. Results: All the extract displayed
significant analgesic effect (P Download Free PDF View PDF Phytomedicine: international journal
of phytotherapy and phytopharmacology Fraxetin and ethyl acetate extract from Lawsonia inermis
L. JI: Supervised and assisted KI on the antileishmanial work and participated in writing the
manuscript.
The in vitro antibacterial and antifungal activities were tested by agar disc diffusion method.
Phytochemical study reveals the presence of Saponins, Tannins, Steroids and Anthraquinones in the
ethanolic extract. The antimicrobial activities of silver nanoparticles were evaluated against different
pathogenic bacterium and fungi. Download Free PDF View PDF Plant-mediated green synthesis of
silver nanoparticles using trifolium resupinatum seed exudate and their antifungal efficacy on
neofusicocum parvum and rhizoctonia solani Samira Salari, M K, Pooya Ghasemi Nejad Almani In
recent years, biosynthesis and the utilization of silver nanoparticles (AgNPs) has become an
interesting subject. This plan t is commonly known as Henna or Mhendi and abund antl y availa ble
in tropical and subtropical areas. The dynamic light scattering studies have revealed an average size
and surface Zeta potential of the NPs as, ?39.5 nm and ?21.6 mV, respectively. The potential
antibacterial and antifungal activities of the AgNPs were evaluated against Bacillus subtilis,
Staphylococcus aureus, Candida kruseii and Trichophyton mentagrophytes. Traditionall y in India,
mehndi is applied to hands and feet. The characterization of AgNPs were analyzed using UV visible
spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier
transform infra-red spectroscopy (FTIR). Leaves Extract by GC-MS and their Antibacterial Potential
Anjana Goel Download Free PDF View PDF STUDY ON ANTIMICROBIAL POTENTIAL AND
PRELIMINARY PHYTOCHEMICAL SCREENING OF LAWSONIA INERMIS LINN pavan
dahake The disease causing bacteria have evolved the genetic ability to transmit and acquire
resistance to drugs used as therapeutic agents. The plant is reported to contain Lawsone, Esculetin,
Fraxetin, and anticancer properties. This investigation was performed at the Quantitative Pathology
Center of the Institute Adolfo Lutz (NPQ-IAL). By this study, the pathogens are isolated from
wound sample. One way to prevent antibiotic resistance is by exploring new bioactive compounds
from traditional medicine which is not based on the existing synthetic antimicrobial agents. The
response of the phytoconstituents resides in E. This plant contain other chemicals such as
isoplumbagin, apigenin, apigenin glycosides, luteolin, luteolin-7 glucosides, p-coumarin and lupeol
among which many are reported for their cytotoxicity and chemopreventive activity against different
type of cancer cell. See Full PDF Download PDF About Press Blog People Papers Topics Job Board
We're Hiring. In Kokan, leaf juice mixed with water and sugar is given as a remedy for
spermatorrhoea. To embed this view, Copy and Paste the following HTML code. Allyl-amine
derivatives of lawsone and lapachol were found potent cytotoxic with an IC 50 values of 23.89 and
16.94 ?M respectively da Silva et al., 2002. Dichloroallyl lawsone, an analog of the lapachol, and
acivicin inhibit the biosynthesis of nucleotide and showed anticancer activity against certain
experimental tumor models Kemp et al., 1986. Dichloroallyl lawsone is an important
chemotherapeutic agent causes cardiac toxicity in the rhesus monkey McKelvey et al., 1979. Weight
of 25mL flask solvent evaporated 73.22gm 91.42gm 108gm 88gm 86.11gm. While viewing any
Flickr photos page, click on the bookmarklet to open the same view on Flickriver. The extract was
filtered and concentrated in vacuo to afford 500 g of an oily extract. The extract characterization was
done by the quantitative analysis of eight phytochemicals using gradient reverse phase HPLC
method. IHC showed sensitivity of 98.51 %, specificity of 1. Download Free PDF View PDF Article
wjpps 1410169597 2014 Dr. Sarika Gupta Download Free PDF View PDF Biosynthesized Silver
Nanoparticle based Hybrid Materials Kiruba Daniel, Muthusamy Sivakumar Download Free PDF
View PDF 228-244 K. Masters’ dissertation, Kent State University, Kent, OH. Young branches are
green in colour and quadrangular which turn red with age. The aim of this study is to determine the
antibacterial activity of Citrus limonum (Lemon) juice and Lawsonia inermis (Henna) leaves
combination extract by using agar well-diffusion method and 96-well microtiter plate for MIC test.
Active analytes were cumulatively trapped on SPE cartridges and the structures elucidated by
analysis of NMR spectra obtained in the HPLC-HRMS-SPE-NMR mode. You can download the
paper by clicking the button above.
TEM images revealed the spherical shape of the silver nanoparticles with an average size of 17 nm.
You can download the paper by clicking the button above. It was found that a minimum of two
hydroxy substituents on the A ring, preferably on position 5, 7, and 8, greatly increased the
antileishmanial potential. Once added to your personalized homepage, just edit widget settings to
select your desired view. The plant is reported to contain carbohydrates, proteins, flavonoids, tannins
and phenolic compound s, alkaloids, terpe noids, quinones, coumarins, xanthones and fatt y acids.
The samples used were pure lemon juice and henna leaves that were extracted by using 100%
methanol. Characteristics of AgNPs were determined using techniques, such as ultraviolet-visible
spectroscopy (UV-vis), Fourier transform infrared (FTIR) analysis, scanning electron microscopy
(SEM), energy-dispersive X-ray spectroscopy and X-ray diffraction (XRD). Loaded cartridges were
dried with pressurized nitrogen gas for 45 min each prior to elution with methanol- d 4. Dr. NN
Chavan Keynote address on ADNEXAL MASS- APPROACH TO MANAGEMENT in the.
Flickriver widget for iGoogle or Netvibes can display almost any Flickriver view - most interesting
today, by user, by group, by tag etc. Download Free PDF View PDF Journal of Food Agriculture and
Environment Antimicrobial properties of Lawsonia inermis syn. Moreover, rhamnolipids and proteins
were identified as stabilising molecules for the AgNPs through Fourier transform-infrared
spectroscopy. Initially, crude methanol extract was tested for its ability to inhibit extracellular
promastigotes of L. Weight of antioxidant in sample 25.24gm 40.54gm 52.84gm 35.28gm 42.26gm.
This work is licensed under a Creative Commons Attribution 4.0 License. A partner of Crossref. The
antioxidant property was studied by radical scavenging (DPPH) assay and cytotoxic activity was
evaluated against A-431 osteosarcoma cell line by MTT assay. Plants, rich in secondary metabolites
and essential oils have been studied during the last few decades for potential source of drug.
Phytochemicals from medicinal plants showing antimicrobial activities have the potential of filling
this need, because their structures are different from those of the more studied microbial sources.
Since the dawn of civilization, plants are essential element of human society and used extensively in
folk medicine for the treatment of innumerous ailments. TLC fingerprinting and ash value estimation
was done for drug standardization. Phytochemical screening shows the presence of alkaloids,
saponins, steroid, flavonoids, tannins, terpenoids and glycosides in the plant extract. IHC showed
sensitivity of 98.51 %, specificity of 1. Also, antifungal efficacy of biogenic AgNPs against two
important plant-pathogenic fungi (R. Preliminary observations were recorded in terms of colour
change of the reaction mixture from yellow to greyish black. Leaves Extract by GC-MS and their
Antibacterial Potential Anjana Goel Download Free PDF View PDF STUDY ON
ANTIMICROBIAL POTENTIAL AND PRELIMINARY PHYTOCHEMICAL SCREENING OF
LAWSONIA INERMIS LINN pavan dahake The disease causing bacteria have evolved the genetic
ability to transmit and acquire resistance to drugs used as therapeutic agents. This plant contain other
chemicals such as isoplumbagin, apigenin, apigenin glycosides, luteolin, luteolin-7 glucosides, p-
coumarin and lupeol among which many are reported for their cytotoxicity and chemopreventive
activity against different type of cancer cell. Unfortunately, because of the inappropriate use of
antibiotics in human and veterinary medicine, certain strains of bacteria and fungi developed the
ability to produce substances which block the action of antibiotics or change their target or ability to
penetrate cells. This is an open-access article distributed under the terms of the Creative Commons
Attribution License (CC BY). Immunological assays can be used to investigate the attraction of
vectors to animals, which may be hosts of these parasitoses. Calyx is with a 0.2 cm tube and 0.3 cm
spread lobes. Fruit is a small brown coloured round capsule.
These solutions were assayed for their in vitro antileishmanial activity toward extracellular
promastigotes. This investigation was performed at the Quantitative Pathology Center of the Institute
Adolfo Lutz (NPQ-IAL). The principal components were Tridecane (7.7%), phytol (10.30%),
Hexadecane (14.88%), Tetradecane (16.77%), Heptadecane (23.48%) Rahmat et al.,2006. Structures
of the reported anticancer molecules from Lawsonia inermis and some of the derivatives of lawsone
is given in Figure 1. The dynamic light scattering studies have revealed an average size and surface
Zeta potential of the NPs as, ?39.5 nm and ?21.6 mV, respectively. The potential antibacterial and
antifungal activities of the AgNPs were evaluated against Bacillus subtilis, Staphylococcus aureus,
Candida kruseii and Trichophyton mentagrophytes. However, antimonials have severe side effects
like myalgia, pancreatitis, cardiac arrhythmia, hepatitis, and accumulation of the drug in liver and
spleen. Leaves aqueous extract of Shahdad ecotype had the highest antioxidant activity compared
with those of the other ecotypes. KK: Took part in the chemical investigation and participated in
writing the manuscript. International journal of cancer Journal international du cancer. The present
study was conducted to evaluate the antimicrobial activity from ethanolic extract against different
life threatening pathogenic microorganisms and screening for various phytochemical constituents of
Lawsonia inermis Linn. A similar level of efficacy for the Butantan surfactant, as compared to
Curosurf was demonstrated by the pulmonary dynamic compliance, ventilatory pressure, and
pressure-volume curve results. Thus, there is an urgent need for new chemical entities for non-toxic
and effective treatment of leishmaniasis ( Brooker et al., 2004; Markle and Makboul, 2004 ). The
mode of action of many such secondary metabolites is known. The characterization of AgNPs were
analyzed using UV visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy
(TEM) and Fourier transform infra-red spectroscopy (FTIR). Fruit opens irregularly and splits into
four sections at maturity and is many seeded. Various secondary metabolites synthesized by plants
are biologically active for humans; and thus, they impart medicinal value to the plant species. To
browse Academia.edu and the wider internet faster and more securely, please take a few seconds to
upgrade your browser. Download Free PDF View PDF Green rapid biogenic synthesis of bioactive
silver nanoparticles (AgNPs) using Pseudomonas aeruginosa Sukumar Karuganti The present work
was focused on isolating a bacterial strain of Pseudomonas sp. TEM images revealed the spherical
shape of the silver nanoparticles with an average size of 17 nm. Henna extract prevent the growth of
bacteria in a dose dependent manner. FTIR measured the possible biomolecules that are responsible
for stabilisation of AgNPs. The active major constituents of subfractions 5, 7, 10, and 13 were
identified by HPLC-HRMS-SPE-NMR directly from the crude extract and the IC 50 values of the
identified, active metabolites were determined. Young branches are green in colour and quadrangular
which turn red with age. The morphology of the nanoparticles was determined by scanning electron
microscopy. Lawsonia inermis (L.) popularly known as Mehndi or Henna, is a cosmetically
renowned plant of the oriental region possesses diverse pharmacological activity including anti-
carcinogenic, antimicrobial, anti-inflammatory, analgesic, antipyretic, hepatoprotective, anti-
tuberculostatic. Current result shows core involvement of plant extracts containing glycosides,
flavonoids, and phenolic compounds played a crucial role in the biosynthesis of AgNPs. While
viewing any Flickr photos page, click on the bookmarklet to open the same view on Flickriver. This
work is licensed under a Creative Commons Attribution 4.0 International License. The in vitro
antibacterial and antifungal activities were tested by agar disc diffusion method. This result may
open important perspectives alternative antimicrobial therapies. The aim of this study is to determine
the antibacterial activity of Citrus limonum (Lemon) juice and Lawsonia inermis (Henna) leaves
combination extract by using agar well-diffusion method and 96-well microtiter plate for MIC test.

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