Printed on: Thu Feb 09 2023, 12:38:32 AM(EST) Status: Currently Official on 09-Feb-2023 DocId: GUID-BBECB3D1-081C-4BAC-9100-AF594B44D49D_2_en-US

Printed by: Dang Van Vu Official Date: Official as of 01-May-2019 Document Type: USP @2023 USPC
Do Not Distribute DOI Ref: kc4cs DOI: https://doi.org/10.31003/USPNF_M89070_02_01
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Table 1 (continued)
Witch Hazel
Time Solution A Solution B
(min) (%) (%)
DEFINITION
Witch Hazel is a clear, colorless distillate prepared from 20 0 100
recently cut and partially dried dormant twigs of Hamamelis
virginiana L. Standard solution: 0.03 mg/mL of tannic acid
Prepare Witch Hazel as follows. Macerate a weighed amount Sample solution: Witch Hazel
of the twigs for about 24 h in about twice their weight of Chromatographic system
water, and then distill until NLT 800 mL and NMT 850 mL (See Chromatography á621ñ, System Suitability.)
of clear, colorless distillate is obtained from each 1000 g of Mode: LC
the twigs taken. Add 150 mL of alcohol to each 850 mL of Detector: UV 280 nm
distillate, and mix thoroughly. Column: 4.6-mm × 15-cm; packing L1
OTHER COMPONENTS Flow rate: 1 mL/min
• ALCOHOL DETERMINATION á611ñ, Procedures, Method I— Injection volume: 25 µL
Distillation Method: 14.0%–15.0% of ethanol Analysis
Samples: Standard solution and Sample solution
CONTAMINANTS Acceptance criteria: If any peak corresponding to the
• NONVOLATILE RESIDUE: Evaporate 100 mL to dryness in a retention time of tannic acid in the Standard solution is
tared dish on a hot plate at 60°. The weight of the residue present for the Sample solution, its peak area is NMT that of
does not exceed 25 mg (0.025%). the tannic acid in the Standard solution.
• LIMIT OF TANNINS
Solution A: 1000 mL of water adjusted with phosphoric Delete the following:

al
acid to a pH of 2.5 ± 0.1
Solution B: Acetonitrile ▲
• ARTICLES OF BOTANICAL ORIGIN á561ñ, Pesticide Residue
Mobile phase: See Table 1. Analysis: Meets the requirements▲ (USP 1-May-2019)
Table 1 SPECIFIC TESTS
• SPECIFIC GRAVITY á841ñ: 0.979–0.983
Time
(min)
0
Solution A
(%)
90
(%)
10
ci
Solution B
• PH á791ñ: 3.0–5.0
ADDITIONAL REQUIREMENTS
• PACKAGING AND STORAGE: Preserve in tight containers, and
5 90 10 avoid exposure to excessive heat.
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