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FEVER PROFILE
SEROLOGY
Test Name Result Biological Reference Method
Range
WIDAL (SLIDE AGGLUTINATION)
S. Typhi Antigen (O) <1:80 < 1:80 Negative Agglutianation
S. Typhi Antigen (H) <1:80 < 1:80 Negative Agglutianation
S. Paratyphi Antigen (AH) <1:80 < 1:80 Negative Agglutianation
S. Paratyphi Antigen (BH) <1:80 < 1:80 Negative Agglutianation
Widal test is a Slide agglutination test employed in serological diagnosis of enteric fever.Timings of test is important as antibodies begin to
arise during end of first week. The titres increase during second, third and fourth week after which it gradually declines. The test may be
negative in early part of first week. A single Widal test is of little clinical relevance due to the number of cross reacting infections, including
malaria. If no other tests (either bacteriologic culture or more specific serology) are available, a four fold increase in the titer (e.g., from
1:40 to 1:160) in the course of the infection, or a conversion from an IgM reaction to an IgG reaction of at least the same titer, would be
consistent with a typhoid infection.
NOTE
1.Individuals vaccinated with typhoid vaccine (TAB) may show moderately elevated titre of all three 'H' antibodies.
2.Repeated subclinical infection may give high titres due to previous antibodies.
3.Treatment with antibiotic such as chloramphenicol before the test gives false negative result for 'O' agglutinin. In that case diagnosis
should be based on the significant elevation of 'H' agglutinin in the paired sera.
4.Patients of chronic active liver disease may give high titre due to failure of antigens in discriminating the specific Ab from the
dysglobulinaemia of chronic active liver disease.
5.Infection with many non-Salmonella organisms like Malaria, Dengue, Miliary Tuberculosis, Endocarditis, Brucellosis, Influenza etc. may
give anamnestic response.
6.Potential carriers of the disease exhibit negative result due to high antibody concentration.
7.Immunological disorders such as Rheumatoid Arthritis, Rheumatic fever or Nephritic Syndrome demonstrate high titre of 'O' and 'H'
agglutinins.
8.Narcotic addicts demonstrate non-specific activity to the Widal test.
9.VI antigen may block the 'O' antigen binding to 'O' antibody, leading to false negative results.
10.In endemic areas people usually show moderately elevated level 'O' and 'H' agglutinins
Limitations : Numerous false positives due to cross reacting antibodies and heterospecific anamnestic responses and false low titres as a
result of partial treatment are observed. This makes clinical correlation with lab findings mandatory.
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Disclaimer:-This report is not for medico-legal purpose. For test performed on specimens received or collected from non-CRL locations, it is presumed that the specimen belongs to the patient named or identified as labeled on the container/test request and such verification has been
carried
out at the point generation of the said specimen by the sender. CRL will be responsible Only for the analytical part of test carried out. All other responsibility will be of referring Laboratory.sample processed and reported at CRL DIAGNOSTICS PVT. LTD , Delhi.
Name of Patient : Mrs. USHA DEVI Test Request ID : 82782309260019
Age/Gender : 25 Yrs/Female Specimen Drawn ON : 26-Sep-2023 10:00AM
Collected AT : CRL COLLECTION POINT (GURUGRAM Specimen Received ON : 26-Sep-2023 02:49PM
Referred BY : Dr. SELF Report DATE : 26-Sep-2023 03:37PM
Sample Type : Whole Blood EDTA - A4850422, - ,Serum - A4850421,EDTA Blood - A4850422
Ref Customer :
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Page 2 of 3
Disclaimer:-This report is not for medico-legal purpose. For test performed on specimens received or collected from non-CRL locations, it is presumed that the specimen belongs to the patient named or identified as labeled on the container/test request and such verification has been
carried
out at the point generation of the said specimen by the sender. CRL will be responsible Only for the analytical part of test carried out. All other responsibility will be of referring Laboratory.sample processed and reported at CRL DIAGNOSTICS PVT. LTD , Delhi.
Name of Patient : Mrs. USHA DEVI Test Request ID : 82782309260019
Age/Gender : 25 Yrs/Female Specimen Drawn ON : 26-Sep-2023 10:00AM
Collected AT : CRL COLLECTION POINT (GURUGRAM Specimen Received ON : 26-Sep-2023 02:49PM
Referred BY : Dr. SELF Report DATE : 26-Sep-2023 03:37PM
Sample Type : Whole Blood EDTA - A4850422, - ,Serum - A4850421,EDTA Blood - A4850422
Ref Customer :
QR CODE
Page 3 of 3
Disclaimer:-This report is not for medico-legal purpose. For test performed on specimens received or collected from non-CRL locations, it is presumed that the specimen belongs to the patient named or identified as labeled on the container/test request and such verification has been
carried
out at the point generation of the said specimen by the sender. CRL will be responsible Only for the analytical part of test carried out. All other responsibility will be of referring Laboratory.sample processed and reported at CRL DIAGNOSTICS PVT. LTD , Delhi.