Answer the following Questions in the Word file I have provided.

Please modify the file

name so that your last name and first initial is the first part of the file name (e.g.,
SmithJExamIIIofflineSection01). Also, before answering these questions, make up a
“code word” so that I can post your semester’s grades and overall grade anonymously.
The code word must only be known to you and me and cannot be used by others to easily
identify you. So, no student id# or Social Security # can be used as a code word.
Finally, please do not confer with others to work on these questions. If you use internet or
other sources to help you, please provide a list of references at the end of this exam. You
have till midnight, Apr. 20 to send me this via e-mail: michael.perlin@louisville.edu
Code Word: Macintosh
1. (Worth 40 pts) Evaluate the following pedigrees. Indicate the type of inheritance likely
for each pedigree (in other words: Dominant vs. Recessive, Sex-linked vs. Autosomal, X-
linked or Y-linked, Sex-limited, Sex-influenced) and your rationale in making that
determination. Also, indicate the genotypes of all individuals shown in each pedigree.
A.
B.

2. A. (40 pts) Bart Simpson found a new phage in the Falls of the Ohio, which he called FCD
(Falls City Dude) phage. He then discovered that he could isolate a conditional lethal mutant
of FCD Phage, called the ruok mutant, which produces smaller plaques than wildtype. The
Permissive condition for this mutant is that it can infect and produce progeny on E. coli A;
the Restrictive condition is that the ruok mutants can infect but not produce progeny on E.
coli C. Wildtype FCD Phage can infect and produce progeny on either bacterium. Having
nothing better to do while his skateboard was being repaired, he isolated several thousand
ruok mutants.
a) How could he map the distance between the mutations in his ruok mutants. Be sure to
describe in detail so Bart can, like, do it, man. If Bart counted 2800 total plaques when
infecting E. coli A with two different ruok mutants and 140 wildtype plaques when infecting
E. coli C with the same two mutants, what is the map distance between these two ruok
mutants? What additional measures should Bart take to make sure that the mutants he is
mapping are close enough to be able to effectively use the Map Distance formula?

b) How could he determine the rate of reversion for any of his ruok mutants? Why is this
important?
c) Give a detailed plan for determining whether Bart's ruok mutants had mutations in the same or
in different genes. Describe in detail.

d) (10 pts) Describe epistasis as shown in Bombay Phenotype, including how the epistasis
affects the ratio of phenotypes produced (in say, an F2 generation). Be sure to show the Punnett
square.

Bonus (1 pt)

Answer A or B, not more than one.

A. Name and briefly describe an important aspect of human genetics you learned about in
this class.

B. Current genetic genealogical studies for the Appalachian descendants of the early
colonists to what eventually became the US demonstrate that they carried DNA haplotypes
(male or female lineages) and genes from which of the following ethnic groups as
“founders”?: 1)Sephardic, 2) Ottoman, 3) North African, 4) none of the above, 5) all of the
above.