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L 7 Immobilized Enzymes Reactors


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The document discusses various types of enzyme reactors that can be used for different applications. It describes batch reactors as the simplest type where the enzyme is placed in a
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 In an enzyme reactor, the highest specific enzyme Chapter 5


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CorONTINUOUS PACKED BED REACTORS
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enzymes and immobilized microbial cells. 6


 In these systems, it is necessary to consider the
pressure drop across the packed bed or column,
and the effect of the column dimensions on the
reaction rate.
 There are three substrate flow possibilities in a
packed bed and they are illustrated below:
 Downward flow method
 Upward flow method
 Recycling method

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 e.g., dialysis membrane, to contain the enzyme in
a chamber into which the substrate moves and the
product moves out.

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 Generally, membrane reactors use a hollow fiber of
200 /lm diameter with 50 /lm thick membranes.

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 Each reactor contains hundreds


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which the enzyme is retained; usually these 6


reactors use soluble enzymes.

 The substrate is kept in the main chamber of the


reactor.

 These reactors can also be used in a continuous


mode and the substrate flow rate is adjusted to
achieve the desired level of reaction.

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AorDVANTAGES AND LIMITATIONS
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(i) easy to establish,
7
(ii) permit the use of more than one enzyme to catalyze a
chain of reactions,

(iii) allow easy replacement of enzymes, and

(iv) are useful in producing small scale (g to kg) quantities.

The chief limitations of these systems are:

(i) regular replacement of membranes adds to cost

(ii) the need for substrate diffusion through the membrane


often limits its applications

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Filtration Unit.
 This type of reactor begins as a typical CSTR.

 However, the product passes through an Ultra


Filtration Unit where the enzyme is removed and
recycled back into the reactor.

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In a combined CSTR/UF reactor the enzymes are immobilized in that they
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can not leave the reactor because of the filtration unit.
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The Ultra Filtration Unit contains a membrane which provides a
semipermeable barrier which allows products and unreacted substrate,7if
there is any, to pass through while holding back the enzyme.

There are other possibilities for similar reactors, such as combined reactor-
separators.

However, the combined CSTR/UF reactor has proven useful for several
types of reactions where a typical immobilized enzyme would not be as
effective.

One example of this is for the conversion of benzylpenicillin to 6-


aminopenicillanic acid by penicillin amidase.

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often, but is very important to consider when 6


studying immobilized enzymes.

 It is very important to chemical engineering


because it allows some substrates to be processed,
which could not be processed using other reactor
types.
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 The recycling method is this


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linear velocity of the substrate solution affects the 6
reaction flow rate.
 This is because the recycling method allows the
substrate solution to be passed through the column
at a desired velocity.
 For industrial applications, upward flow is generally
preferred over downward flow because it does not
compress the beds in enzyme columns as
downward flow does.
 When gas is produced during an enzyme reaction,
upward flow is preferred.

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 If the entire product stream is recycled back to the


inlet stream, then it is called a total recycle reactor.
 This can obviously only be used in a batch process,
because if the entire product stream is recycled
back into the reactor in a continuous reactor, the
volume of the reactor would increase to infinity.

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This type of rector is used when you have a substrate that
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These reactors continue to move the same substrate
through the reactor so that the effective contact time is high 6
enough to allow the substrate to be processed.

 Recycle reactors also allow the reactor to operate at high


fluid velocities.

This is important because it minimizes the bulk mass


transfer resistance to the transport of the substrate.

A recycle reactor is simply a reactor, such as a CSTR or


fluidized-bed reactor, with a recycle stream.
 .

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molecular weight and a product of low molecular 6


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 The enzyme used here is soluble, no improvement


in the stability of the enzyme can be expected.

 A hollow fiber device can also be used and its


characteristics are essentially the same as those of
an ultrafiltration membrane

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