(12)1<7!'(-2+1-(1297!)=;!91<(--(4$;129:;!'(3'&1!;$!$;$)(3U&)&!2&!.1-5891</!)&2)!&'1.&.

&)(1<T6!2Y4<(1286B!)-!)&2)!v[^klwlmB!+&*5;&1!7)1!-1!+Y-!-9/+
%&*#*##0"
&* ) "..:! $'-9-;:;&)$1 ! 2& ! 9&4$0Y)&(- ! '-2-2)7 ! Dehydrated culture medium

&0329$)$ ! '-,
eNf ! &'1),45/;-;)$1 ! 9& ! )- ! 2Y2)89$ !,-./ 0 121345 0 6789! 2,9>=;-Y; ! 9
Rev. 0 - 3rd Ed. 2004

MbcM!d! W
;-;)$1 ! 5(8219-'-1:;)$6 ! Z[\ ! ]^[Z_ ! E`aMbcM ! d ! e<=+W ! C@@NPNB ! C?@NPf ! <$1 ! .1-5891</ !
Page 1 of 2

=28!)86!$;$4;:(1286!)=;!91<(-.*=;W!X$!Z[\!]^[Z_!E`aMbcM!d!'&(135-,;!)$!,01</!<$0013
TETRATHIONATE BROTH BASE
hkGlmn_o ! Lghi ! <$1
Enrichment ! G_pi[I[Z_
medium ! LghiW
for isolating ! X$ ! $'-)&0329$)$
Salmonella ! )-, ! $;)1.1-4(/99$)-6
typhi and other salmonellae ! 9& ! Ei[m !
from feces, sewage,
-Y; ! 9& ! etc.
)$ ! $'-)&0329$)$ ! )86 ! 9&%7+-, ! rhq_i ! _p ! h^W ! ! '-, ! 2,;12)/)$1 ! $'7 ! )8; ! Fll
eDf

-9&*)$1! =6! &'1<*;+,;-! 2Y9>=;$! 9&! )8;! 125Y-,2$! ;-9-%&

hp[lm!eFsLf!<$1!$'7!)8;!dhp[lmh^!GlII[pp__!li!G^[m[kh^!Jhtlihplio!cphmZhiZ]edGGJcf!EWc
-4T!)-,!)&2)!2)$)12)1<T6!98!'$($9&)(1<T6!2Y4<(1286B!)-!)&2)!v[^klwlmB!+&*5;&1!7)1!-1!+Y-!-
2&16! {?|B !41}$,)7! +&;! $'$1)&*)$1! 8! +1$%&2197)8)$! )86
TYPICAL FORMULA (g/l)
3(-,; !Calcium
289$;)1</K
Peptospecial
Carbonate
! xy@B@DNCW
5.0
10.0
!!4-%&*#*##0" ) "..:! $'-9-;:;&)$1 ! 2& ! 9&4$0Y)&(- ! '-2-2
1$! 2,2<&,T! 91$6! 5(T286! '-,!
06789!2&!25328!9&!)$!Z[\!]^[Z_!E`aMbcM!d! W '(3'&1! ;$! 5(8219-'-1&*)$1!
eNf
Bile Salts 1.0
Sodium Thiosulphate 30.0

&'$44&09$)1<T!5(T28!<$1!'(3'&1!;$!5(8219-'-1&*)$1!2)-!&(
"+B!(& Final pH = 8.4 ± 0.2 at 25°C.*
*Misured before iodine solution is added.
7;! ,-./ 0 121345 0 6789B !+&;! )$U1;-9&*)$1! =6! &'1<*;+,;-! 2Y9>=;$! 9&! )8;! 125Y-,2$! ;-9
$1!9&!&4<&<(193;&6!$28')1<36!<$1!$2>$0&*6!9&%7+-,6!2&!253
DIRECTIONS
.0$.&(36!
Suspend-,2*&6! 2&! in2,4<&;)(:2&16!
46.0 g of powder {?|B
980 ml of distilled or deionized water.!41}$,)7! +&;! $'$1)&*)$1! 8! +1$%&2197)8)$!
Cool to below 45°C and add 20 ml of Iodine supplement (code 81009) immediately before use.
$6WX-! ,-./012134506789!
Mix continuously whilst dispensing 10 &*;$1! 91$!into
ml volumes 2,2<&,T! 91$6!
sterile tubes. Use 5(T286!
the complete '-,!
medium (with'(3'&1! ;$!
added iodine) 5(8219-'-1&*
on the day of
1<T!5(T28!'4);'-&*B!'(--(*~&)$1!41$!&'$44&09$)1<T!5(T28!<$1!'(3'&1!;$!5(8219-'-1&*)$1!2)-
preparation.

0080$!&<'$1+&,93;-!'(-2='1<7!<$1!9&!&4<&<(193;&6!$28')1<36!<$1!$2>$0&*6!9&%7+-,6!2&
Iodine supplement
&6!-,2*&6W!
1 Vial contents (each vial is sufficient for 500 ml of medium):
Iodine 6.0 g
(51<T!)-,!2,2<&,$2*$W !‚&;!'(3'&1!;$!>,0/22&)$1!<-;)/!
Potassium iodide
Distilled water
5.0 g
20.0 ml

!+1$<,9/;2&16W!ƒ'7!$,)36!)16!2,;%T<&6!)-!'(-z7;!125Y&1!
)-!2&!%&(9-<($2*$!N€R!•G
DESCRIPTION !2)8;!$(51<T!)-,!2,2<&,$2*$W !‚&;!'(3'&1!;$!>,0/22&)$1!<-;
$6!<$1!'(3'&1!;$!$'->&Y4-;)$1!-1!+1$<,9/;2&16W!ƒ'7!$,)36!)16!2,;%T<&6!)-!'(-z7;!125Y
1<3)$W!„8;!)$!5(8219-'-1&*)&!'3($;!$,)T6!)86!89&(-98;*$6
TETRATHIONATE BROTH BASE is an American formulation which complies with the description given in the US
Pharmacopoeia for the enrichment of specimens undergoing examination for salmonellae.
;*$!0TU86!'-,!$;$4(/>&)$1!2)8;!&)1<3)$W!„8;!)$!5(8219-'-1&*)&!'3($;!$,)T6!)86!89&(-98
The selectivity of the medium depends on the ability of thiosulphate and tetrathionate in combination to suppress commensal
0-*=286W
'-1&*)&!&/;!'$(-,21/~-,;!289&*$!$00-*=286W
coliforms organisms.
Organisms which possess the enzyme tetrathionate reductase grow in the medium.
Salmonella and Proteus species possess the enzyme; Escherichia coli and shigellae do not.
C*0)%<0$:*&(@%'%(*@!;%<0<"(>%<
Proteus can be inhibited by adding 40 μg/ml of novobiocin to the incomplete medium before the addition of iodine.

!"(>%<
5(T28TECHNIQUE
! )- !,-./ 0 121345 0 6789! <$1 ! )$ ! ,01</ ! '-, ! T(%$; ! 2& ! &'$>T ! 9& ! )- ! +&*49$
*;-;)$1!<$1!;$!$'-((*')-;)$1!2Y9>=;$!9&!)16!2,;T%&16!)&5;1<36!&(4$2)8(*-,!41$!)8;!$'-
Inoculate the broth with 1-2 g of the specimen and mix thoroughly to disperse the sample. Incubate at 36+/-1°C for 18-24 hours
! <$1and! )$ ! ,01</ ! '-, ! T(%$; ! 2& ! &'$>T ! 9& ! )- ! +&*49$ ! '
then subculture on a selective medium for salmonella isolation.
(1…8!'1%$;:6!9-0,293;-,!,01<-YW!
>=;$!9&!)16!2,;T%&16!)&5;1<36!&(4$2)8(*-,!41$!)8;!$'-0Y9
(+&*
QUALITY CONTROL
Dehydrated medium
Appearance: free-flowing, homogeneous.
Color: white to off-white, may have a slight greenish tint.
Prepared medium
>IJKLGM &NOLPNQORQ
/.3SW0121345
Appearance: supernatant over a heavy white precipitate. QA?CD N@!-%"-"
Color: nearly colorless to yellow.
Incubation conditions: 36 ± 1 °C for 18-24 hours.
&0&<@X%"?;
>IJKLGM &NOLPNQORQ
Microorganism ATCC Growth
(1<T Escherichia coli „8;!)-
25922 none ‡&(1&579&;-!)86
4;=2)1<TSalmonella typhimurium 14028 good #$)$2<&,$2)T6!
&'$;$5(8219-'-1&*)& 2,2<&,$2*$6
2<&,T!'4);'-&*
STORAGE QA?CD N@!-%"-"
ˆY%($,2)-B
The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and
%976 use it before the expiry date on the label or until signs of deterioration or contamination are evident. ‡(-2-5TB!+&*)&!)16
5&1(12)&*)& ‰(T28!3=6! Store prepared tubes,
$074-,!without iodide-iodide solution, at 2-8 °C. Iodide-iodide solution can then be added at the time of use -+84*&6!5(T286
to the quantity of medium
'(-2&<)1</
needed.

LIOFILCHEM s.r.l.
Via Scozia, Zona Ind.le - 64026, Roseto D.A. (TE) - ITALY
#$)$2<&,$2)T6!
‡&(1&579&;-!)86 IVD ‡&(1
1&*)& Phone +390858930745 Fax +390858930330
2,2<&,$2*$6
Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net %&(9
!YYZHKLQ0[0\]
‡(-2-5TB!+&*)&!)16 #=+
‰(T28!3=6!
 %&(97)8)$6!<$1!'(3'&1!;$!$'->&Y4-;)$1!-1!+1$<,9/;2&16W!ƒ'7!$,)36!)16!2,;%T<&6!)-!'(-z7;!125
$'-0,9$*;-;)$1!<$1!;$!$'-((*')-;)$1!2Y9>=;$!9&!)1
(12)1<7!'(-2+1-(1297!)=;!91<(--(4$;129:;!'(3'&1!;$!$;$)(3U&)&!2&!.1-5891</!)&2)!&'1.&.
&)(1<T6!2Y4<(1286B!)-!)&2)!v[^klwlmB!+&*5;&1!7)1!-1!+Y-!-9/+
89&(-98;*$!0TU86!'-,!$;$4(/>&)$1!2)8;!&)1<3)$W!„8;!)$!5(8219-'-1&*)&!'3($;!$,)T6!)86!89&(-98
)8;!$'7((1…8!'1%$;:6!9-0,293;-,!,01<-YW!
5(8219-'-1&*)&!&/;!'$(-,21/~-,;!289&*$!$00-*=286W

%&*#*##0"
&* ) "..:! $'-9-;:;&)$1
+'%::(C*0)%<0$:*&(@%'%(*@!;%<0<"(>%< ! 2&<$1!! )$9&4$0Y)&(-
'+:%<&(+&* ! '-2-2)7 !
Dehydrated culture medium

&0329$)$ ! '-, ! &'1),45/;-;)$1 !

„&)/ ! )8 ! 5(T28 ! )-9& !
!,-./ )- ! 2Y2)89$
0 121345 0 6789!!,-./ !0,01</
121345 0 6789!
! '-, ! T(%$; 2,9>=;-Y;
! 2& ! &'$>T ! 9
! 9& ! )- ! +&*49$
Rev. 0 - 3rd Ed. 2004

MbcM!d! eNf
W $'-0,9$*;-;)$1!<$1!;$!$'-((*')-;)$1!2Y9>=;$!9&!)16!2,;T%&16!)&5;1<36!&(4$2)8(*-,!41$!)8;!$'-
;-;)$1 ! 5(8219-'-1:;)$6 ! Z[\ ! ]^[Z_ ! E`aMbcM
'DEFGH
! d ! e<=+W ! C@@NPNB ! C?@NPf ! <$1 ! .1-5891</ !
STUT0V4/.3SW0121345
)8;!$'7((1…8!'1%$;:6!9-0,293;-,!,01<-YW!
Page 2 of 2

=28!)86!$;$4;:(1286!)=;!91<(-.*=;W!X$!Z[\!]^[Z_!E`aMbcM!d!'&(135-,;!)$!,01</!<$0013
hkGlmn_o ! Lghi ! <$1'+:%<&(+&*
REFERENCES
! G_pi[I[Z_
'DEFGH
1. The United States Pharmacopeia
'(;+>+&0&<@X%"?;
! LghiW ! X$ ! $'-)&0329$)$
XXI (1985). Microbial Limit Tests. Rockville, Md.
! )-, ! $;)1.1-4(/99$)-6&NOLPNQORQ
>IJKLGM
! 9& ! Ei[m !
-Y; ! 9& 2.! )$ ! $'-)&0329$)$ ! )86(1980).
! 9&%7+-, ! rhq_i †$)(1<T
! _p+1$4;=2)1<T
! h^W ! !of'-,
eDf
! 2,;12)/)$1 15th! ed.
$'7 ! )8; ! Fll
American STUT0V4/.3SW0121345
Public Health Association Standard methods for the examination QA?CD
water and „8;!)-
wastewater. N@!-%"-"
APHA

-9&*)$1! =6! &'1<*;+,;-!

'(;+>+&0&<@X%"?; 2Y9>=;$! 9&! )8;! 125Y-,2$! ;-9-%&
hp[lm!eFsLf!<$1!$'7!)8;!dhp[lmh^!GlII[pp__!li!G^[m[kh^!Jhtlihplio!cphmZhiZ]edGGJcf!EWc &'$;$5(8219-'-1&*)&
Inc. Washington DC.
2,2<&,T!'4);'-&*
-4T!)-,!)&2)!2)$)12)1<T6!98!'$($9&)(1<T6!2Y4<(1286B!)-!)&2)!v[^klwlmB!+&*5;&1!7)1!-1!+Y-!-
PRESENTATION
2&16! {?|B !41}$,)7!
3(-,; ! 289$;)1</K
Product
†$)(1<T
! xy@B@DNCW
+1$4;=2)1<T +&;!
!!4-%&*#*##0"
2,2<&,T!'4);'-&*
$'$1)&*)$1!
„8;!)-
) "..:!
S(1%976
8!
$'-9-;:;&)$1
<$)$074-,!
&'$;$5(8219-'-1&*)&
+1$%&2197)8)$!
!
#$)$2<&,$2)T6!2&
ˆY%($,2)-B
! ‡&(1&579&;-!)86
9&4$0Y)&(-
2,2<&,$2*$6 ! '-2-2
5&1(12)&*)& )86
'(-2&<)1</
1$! 2,2<&,T! 91$6! 5(T286! '-,!
06789!2&!25328!9&!)$!Z[\!]^[Z_!E`aMbcM!d! ˆY%($,2)-B '(3'&1! ;$! 5(8219-'-1&*)$1!
W
TETRATHIONATE BROTH BASE (10.8 l) eNf610183 500 g
S(1%976 ‡(-2-5TB!+&*)&!)16
TETRATHIONATE BROTH <$)$074-,!
BASE (2.1 l) 5&1(12)&*)&
620183 ‰(T28!3=6! 100 g -+84*&6!5(T286
&'$44&09$)1<T!5(T28!<$1!'(3'&1!;$!5(8219-'-1&*)$1!2)-!&(
"+B!(& '(-2&<)1</

7;! ,-./ 0 121345 0 6789B !+&;! )$U1;-9&*)$1! =6! &'1<*;+,;-! 2Y9>=;$! 9&! )8;! 125Y-,2$! ;-9
$1!9&!&4<&<(193;&6!$28')1<36!<$1!$2>$0&*6!9&%7+-,6!2&!253
TABLE OF SYMBOLS

.0$.&(36! -,2*&6! 2&! 2,4<&;)(:2&16! {?|B !41}$,)7! +&;! $'$1)&*)$1! 8! +1$%&2197)8)$!

$6WX-! ,-./012134506789! &*;$1! 91$! 2,2<&,T! 91$6! 5(T286! '-,! '(3'&1! ;$! 5(8219-'-1&*!YYZ
1<T!5(T28!'4);'-&*B!'(--(*~&)$1!41$!&'$44&09$)1<T!5(T28!<$1!'(3'&1!;$!5(8219-'-1&*)$1!2)-
!YYZHKLQ0[0\]
0080$!&<'$1+&,93;-!'(-2='1<7!<$1!9&!&4<&<(193;&6!$28')1<36!<$1!$2>$0&*6!9&%7+-,6!2&
&6!-,2*&6W!
(51<T!)-,!2,2<&,$2*$W !‚&;!'(3'&1!;$!>,0/22&)$1!<-;)/!
!+1$<,9/;2&16W!ƒ'7!$,)36!)16!2,;%T<&6!)-!'(-z7;!125Y&1!
)-!2&!%&(9-<($2*$!N€R!•G !2)8;!$(51<T!)-,!2,2<&,$2*$W !‚&;!'(3'&1!;$!>,0/22&)$1!<-;
$6!<$1!'(3'&1!;$!$'->&Y4-;)$1!-1!+1$<,9/;2&16W!ƒ'7!$,)36!)16!2,;%T<&6!)-!'(-z7;!125Y
1<3)$W!„8;!)$!5(8219-'-1&*)&!'3($;!$,)T6!)86!89&(-98;*$6
;*$!0TU86!'-,!$;$4(/>&)$1!2)8;!&)1<3)$W!„8;!)$!5(8219-'-1&*)&!'3($;!$,)T6!)86!89&(-98
0-*=286W
'-1&*)&!&/;!'$(-,21/~-,;!289&*$!$00-*=286W
C*0)%<0$:*&(@%'%(*@!;%<0<"(>%<
!"(>%<
5(T28 ! )- !,-./ 0 121345 0 6789! <$1 ! )$ ! ,01</ ! '-, ! T(%$; ! 2& ! &'$>T ! 9& ! )- ! +&*49$
*;-;)$1!<$1!;$!$'-((*')-;)$1!2Y9>=;$!9&!)16!2,;T%&16!)&5;1<36!&(4$2)8(*-,!41$!)8;!$'-
! <$1 ! )$ ! ,01</ ! '-, ! T(%$; ! 2& ! &'$>T ! 9& ! )- ! +&*49$ ! '
(1…8!'1%$;:6!9-0,293;-,!,01<-YW!
>=;$!9&!)16!2,;T%&16!)&5;1<36!&(4$2)8(*-,!41$!)8;!$'-0Y9
(+&*
>IJKLGM &NOLPNQORQ
/.3SW0121345 QA?CD N@!-%"-"

&0&<@X%"?;
(1<T
4;=2)1<T
>IJKLGM
„8;!)-
#$)$2<&,$2)T6!
&NOLPNQORQ
‡&(1&579&;-!)86
&'$;$5(8219-'-1&*)& 2,2<&,$2*$6
2<&,T!'4);'-&* QA?CD N@!-%"-"
ˆY%($,2)-B
%976 ‡(-2-5TB!+&*)&!)16
5&1(12)&*)& ‰(T28!3=6!
$074-,! -+84*&6!5(T286
'(-2&<)1</

LIOFILCHEM s.r.l.
‡&(1&579&;-!)86
Via Scozia, Zona Ind.le - 64026, Roseto D.A. (TE) - ITALY
#$)$2<&,$2)T6! IVD ‡&(1
1&*)& Phone +390858930745 Fax +390858930330
2,2<&,$2*$6
Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net %&(9
!YYZHKLQ0[0\]
‡(-2-5TB!+&*)&!)16 #=+
‰(T28!3=6!