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Standard XII
Biology Practical Examination Skeleton Question Paper
Time: 3 Hours Max Marks: 30

PART - A
Q. 1. Make a temporary potato osmoscope to study osmosis. Sketch and label the
diagram showing the change in the water level. (4)
OR
Make a temporary slide to study plasmolysis. Sketch and label the diagram. (4)
OR
Make a temporary mount of leaf epidermis to study the structure and
distribution of stomata. Sketch and label structural apparatus. (4)
OR
Perform the experiment to isolate DNA from given sample. Show your result to
the examiner. (4)
Q. 2. Dissect and display floral whorls. Take a T.S. of ovary to show number of
chambers. Sketch and label the diagram of the same. (3)
OR
Make a temporary preparation to show pollen germination. Draw neat and
labelled diagram of the same. (3)
OR
Calculate the population density / frequency of given species by quadrat method.
Q.3. Perform the suitable test to compare the given water sample for their pH
and clarity. Make a note on their pH and clarity. (3)
OR
Perform the suitable test to compare the given soil sample for their pH and type.
Make a note on their pH and soil type. (3)
OR
Make a temporary mount of given water sample. Prepare a report for the
presence of different planktons. (3)
Q.4 Identify the spots ‘A’ to ‘J’ as per the instructions. (1 mk each ) (10)
Q.5 Project report. (5)
Q.6 Viva voce based on first four questions. (2)
Q.7 Certified Journal. (3)
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Q. 1. Make a temporary potato osmoscope to study osmosis. Sketch and label

the diagram showing the change in the water level. 4 mks
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OR
Q. 1. Make a temporary slide to study plasmolysis. Sketch and label the
diagram. 4 mks
A) Preparation of slide to show plasmolysis - 2 mks

B) Diagram of the plasmolysed cell – 1 mk

C) Observation and inference – 1 mk

Dig : Plasmolysed cell

Observations :

It is observed that, there is shrinkage of cytoplasm which moves away from the
cell wall. It becomes concentrated to the corner of the cell wall. This is called
plasmolysis.
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OR
Q. 1. Make a temporary mount of leaf epidermis to study the structure and
distribution of stomata. Sketch and label structural apparatus. 4 mks

A) Preparation of slide to show stomata - 2 mks

B) Diagram of the stomata with label – 1 mk

C) Observations – 1 mk

Fig : Dicot Stomata

Observations :
1. Under the microscope, The peel of leaf shows large number of stomata which
is surrounded by epidermal cells.

2. Stomata shows two guard cells which are in kidney shaped. It encloses small
opening called stomatal opening.

3. Stomata play an important role in transpiration.

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Q.2. Dissect and display floral whorls. Take a T.S. of ovary to show
number of chambers. Sketch and label the diagram. 3 mks

A) Display of floral whorls on paper with names – 1 mk

B) Preparation of slide of T. S. of ovary - 1 mk

C) Draw the diagram of T. S. of ovary in the answer paper - 1 mk

Fig : T. S. of ovary
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OR
Q.2. Make a temporary preparation to show pollen germination. Draw
neat and labeled diagram of the same. 3 mks

A) Preparation of slide - 1 mk
l) Remove the anther from Catharanthus ( Vinca rosea) flower with the foreceps.
2) Tease it in 35% sucrose solution and keep it for 5 -10 mins.
3) Remove the trashes and cover with coverslip. Observe under microscope.

B) Draw Labelled diagram – 1 mk

C) Observations – 1 mk

Fig : Pollen germination

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OR
Q.2. Calculate population density / frequency of any one of the species by
quadrat method. 3 mks
A) Observation table and calculation of density / frequency of the species – 2
mks

B) Result - 1 mk

A) Observation table :

Name No. of individual per Total Total Total Population Frequncy

of quadrat No. of No. of no. of Density Percentage
Plant Species quadrat quadrat
species ( N) inwhich studied N/B A/B x 100
species (B)
occurred
(A)
Q1 Q2 Q3 Q4

B) Result :

1) The population density of the given species is …………………….

2) The frequency percentage of the given species is …………………

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Q. 3. Perform the suitable test to compare the given water samples for their
pH and clarity. Draw observation table and make a note of their pH and
clarity. 3 mks

Two water samples varying in pH and clarity to be given to each student.

A) Detecting pH of each sample - 2 mks

B) Detecting clarity of each sample ( Clear, turbid, highly turbid ) - 1 mk

pH
Water sample (Acidic / Basic / Clarity
Neutral )

I
II
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OR
Q.3. Perform the suitable test to compare the given soil samples for their
soil type. Draw observation table and note on soil type. 3 mks

Two soil samples varying in pH and type to be given to each student.

A) Detecting pH of each sample - 1 mk each
B) Detecting soil type of each sample ( sandy, clay, loam ) – 2 mks

Soil sample pH % of % of % of Soil

(Acidic / sand silt clay Type
Basic /
Neutral )
I
II
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OR
Q. 3. Make a temporary mount of given water sample. Make a report of the
presence of different plankton. 3 mks

Preparation of slide – 2 mks

Put a drop of water sample on the slide. Cover with coverslip. Observe under
microscope.

Observations – 1 mk
Various living microorganisms observed under microscope such as paramecium,
diatoms, algae, spirogyra, and some plankton such as copepods, daphnia,
rotifers, larvae, etc.

PART - B
Q.4. Identify the spots ‘A’ to ‘J’ as per the instructions. (1 mk each) 10 mks
A) Correct identification of specimen - ½ mk
B) Description as per instructions ( 3 points ) – ½ mk
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SPOT A

The given photograph is of Salvia flower

and it shows insect pollination.
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SPOT A

The given photograph is of Maize and it

shows wind pollination.
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SPOT B
The given photograph shows separation
of plant pigments by paper
chromatography experiment.
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SPOT C
The given photograph is of
Calotropis (Ruee).
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SPOT C
The given photograph is of
Opuntia.

SPOT C
The given photograph is of Acacia arabica (Babhool).
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SPOT C
The given photograph is of
Eichhornia (water hyacinth).

SPOT C
The given photograph is of
Hydrilla.
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SPOT C
The given photograph is of Typha (Cat tail).

SPOT D
The given photograph is of T.S. of testis of mammal.
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SPOT D
The given photograph is of T.S. of ovary of mammal.

SPOT D
The given photograph is of V.S. of blastula of mammal.

SPOT E
The given photograph is of pedigree chart showing Rolling of
tongue.
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SPOT E
The given photograph is of pedigree chart showing Widow’s
peak.

SPOT E
The given photograph is of pedigree chart showing Blood
groups.

SPOT E
The given photograph is of pedigree chart
showing Colorblindness.
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SPOT F
The given photograph is of causative pathogen
Plasmodium.

SPOT F
The given photograph is of causative pathogen
Entamoeba.
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SPOT F
The given photograph is of causative pathogen
Ascaris.

SPOT F
The given photograph is of causative pathogen
Ringworm.
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PART - C

Q.5 Project report. 5 mks

Q.6 Viva voce based on first four questions. 2 mks
Q.7 Certified Journal. 3 mks