Choose the correct answer.

Name:

1. Fluoresceine is a What light source is used in most flow cytometers?

a. fluorochrome, b. FITC, c. fluorescing dye, d. all the three

2. What is the sequence of the three main systems in a flow cytometer?

a. Fluidics, optics and electronics, b. Optics, fluidics and electronics
c. Electronics, optics and fluidics, d. Fluidics, electronics and optics

3. What type of biological sample is best suited for flow cytometric analysis?
a. Single cell suspension, b. Tissue sample, c. Cell suspension, d. Cells

4. Cell-sorter is not to isolate a pure population of a particular cell population.

True, b. False

5. All flow cytometric measurements can be made simultaneously on a single cell.

a. True, b. False

6. Particles must be in single-cell suspension before flow cytometric analysis.

a. True, b. False

7. PMT stands for a. Photon-multiplier tube, b. Photo-multiplier tube, c. Photo muscle

tube, d. none of the three

8. Increasing sample pressure ______________ the sample flow rate

a. Increases, b. decreases, c. maintains the same, d. blocks

9. In Flow cytometry cell pass through:

a. Cell tube, b. flow cell, c. cell pipe, d. direct pass

10. Light scatter collected at 90 degrees to the laser beam is called:

a. Forward scattered light, b. Side scattered light, c. Direct light, d. Indirect light

11. In cells ATPs are generated in: a. mitochondria, b. nucleus, c. membrane, d. ribosome

12. ATP is generated in: a. mitochondria, b. nucleus, c. cell membrane, d. ribosome

13. Bacterial cell is a eukaryote: a. True, b. False

14. Nucleolus is part of the: a. nucleus, b. ribosome, c. cytoplasm, d. RNA

15. Rough endoplastic reticulum contains: a. golgi, b. ribosomes, c. nucleolus d. enzyme

16. Prokaryots do not have: a. nuclear membrane, b. DNA, c. cytoplasm, d. cell membrane

17. Centrioles are part of: a. nucleus, b. ribosome, c. cytoplasm, d. centrosome

18. Ribosomes are for synthesis of: a. protein, b. fat, c. carbohydrate, d. all the above

19. Post office of the cell: a. cell, b. nucleus, c. ATP, d. golgi

20. Robert Hooke discovered: a. cell, b. nucleus, c. ATP, d. DNA