COLLEGE OF MEDICAL LABORATORY SCIENCE

Name: ________________________________ Date: ______________

Year and Section: _______________________

Activity No. 6
THREE-PHASE BLOOD COMPATIBILITY TESTING

Background:

Compatibility test or cross matching is performed subsequent to the ABO grouping and
Rh typing of the recipient’s and donor’s blood. It is the final criterion as to the suitability
of a particular donor blood for a particular recipient.
The recipient’s blood is obtained fresh while the donor’s blood is obtained from the pilot
tube. CPDA-1 anticoagulant donor’s blood should not be more than 35 days and
constantly stored at 4°C.

Principle:

Serum of the recipient is tested against the red cells of the donor under different
conditions in order to establish their cross matching or non-agglutination. Agglutination
in any of the conditions indicates the presence of incompatible antibody in patient,
natural or immune.

Reagents/ Samples/ Supplies:

Blood samples
LISS
AHG
Serologic Centrifuge
Dry Bath Incubator
Test Tubes / Test Tube Racks
Permanent Pen
Calibrated Plastic Pipets
0.9 Normal Saline Solution
Serological Centrifuge
Dry Bath
Typing Sera
Glass Slides

Procedure:
1. Label test tubes properly.
2. Add 2 drops of patient’s serum/plasma
3. Add 1 drop of 3-5% donor red cell
4. Centrifuge for 1 minute
5. Read and Record the result of the IMMEDIATE SPIN (IS)
6. Add 2 drops of LISS
7. Mix and incubate for 15 minutes at 37 degrees Celsius (dry bath)
8. After incubation, centrifuge for a minute
9. Read and record result – THERMO PHASE (37 degrees Celsius)
10. Wash 3-4x with large amount of NSS
11. After the last washing, blot the tube in a gauze pad/ tissue paper to absorb excess
saline
12. Add 2 drops AHG reagent

Minante I, Cauayan City, Isabela

13. Spin for another minute and read immediately macroscopically/microscopically – AHG
PHASE
14. Record results.

Draw your compatibility testing set-up.

Minante I, Cauayan City, Isabela

 Patient’s Blood Type Interpretation

Anti-A Anti-D Anti-B A1 cells B cells

Donor 1 Blood Type Interpretation

Anti-A Anti-D Anti-B A1 cells B cells

Donor 2 Blood Type Interpretation

Anti-A Anti-D Anti-B A1 cells B cells

Donor Number Blood Type Crossmatch Interpretation/

Remarks
IS 37C AHG

Interpretation Remarks:
No hemolysis/ No agglutination/ COMPATIBLE
With hemolysis/ with agglutination/ INCOMPATIBLE

Questions:

1. What is Major Compatibility Testing?

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2. What are the three phases in compatibility testing? Discuss each phase.

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Minante I, Cauayan City, Isabela

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3. What is the importance of performing compatibility testing between the

patient’s and donor’s blood?

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4. What is the purpose of using Anti Human Globulin (AHG) or Coombs Test in

compatibility testing?

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5. Can you issue an uncrossmatched blood to a patient in case of emergency?

What will be your restrictions?

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Minante I, Cauayan City, Isabela