EDEXCEL AS BIOLOGY 2024 PLATINUM BUSINESS ACADEMY DR SHAKEEL JALEEL

1.Use a semi-quantitative method with Benedict’s reagent to estimate the

concentrations of reducing sugars and with iodine solution to estimate the
concentrations of starch, using colour standards.

Objectives:

o To understand what is meant by a semi-quantitative test

(by observing colour change on a scale it is possible to estimate the concentration of
the reducing sugar)
o To be able to estimate concentrations of reducing sugars using Benedict’s
reagent.

o To be able to estimate concentrations of starch using iodine solution.

o To develop the skills needed to plan an investigation using dilutions.

Example: Describe how a valid and reliable investigation can be carried out by the
nutritionist to determine the reducing sugar content of the milk brands

o Reducing sugar content of the milk brands can be determined by using Benedict’s
reagent.
o Take 5cm3 of milk each from different brands in different test tubes of same
diameter and add 1cm3 of Benedict’s reagent to each of this.
o Place these test tubes in a water bath kept at 700C and start the stopwatch.
o After 2 minutes take the test out from the water bath and observe the colour
change from blue to green to red.

O Use a standard colour chart to identify concentration of reducing sugar in each

milk product

o Repeat at least three times for each milk brand and find the mean concentration.

o Throughout the experiment control the variables such as temperature, using

temperature- controlled water bath and volume of Benedict’s reagent used using
graduated pipette.

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EDEXCEL AS BIOLOGY 2024 PLATINUM BUSINESS ACADEMY DR SHAKEEL JALEEL

Example: Describe how a valid and reliable investigation can be carried out by the
nutritionist to determine the protein content of the milk brands

o Protein content of the milk brands can be determined by using Biuret’s reagent
o Take 5cm3 of milk each from different brands in different test tubes of same
diameter and add 1cm3 of Biuret’s reagent to each of this.
o After 30 seconds, compare the purple color in the test tube
o After 2 minutes take the test out from the water bath and observe the colour
change from blue to green to red.

O Use a standard colour chart to identify concentration of protein in each milk

product

o Repeat at least three times for each milk brand and find the mean concentration.

o Throughout the experiment control the variables such as temperature, using

temperature- controlled water bath and volume of milk and biuret’s reagent used
using graduated pipette.

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EDEXCEL AS BIOLOGY 2024 PLATINUM BUSINESS ACADEMY DR SHAKEEL JALEEL

Example: Describe how a valid and reliable investigation can be carried out by the
nutritionist to determine the starch content of different potato species

o Starch content of the potatoes can be determined by using Iodine solution

o Use same extraction method, same mass of potato, same volume of water added
to crushed potato
o Measure equal volumes of potato solution using a graduated pipette in same
diameter
o Add equal volumes of iodine solution to each test tube.

O Use a standard colour chart to identify concentration of starch concentration in

each potato extract

o Repeat at least three times for each potato species and find the mean
concentration.

o Throughout the experiment control the variables such as temperature, using

temperature- controlled water bath and volume of potato extract using a graduated
pipette

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