GOST 12.4.

152-85

Occupational Safety Standards System

(OSS). Artificial leather.
Methods for the determination of mushroom
resistance

GOST 12.4.152-85
Group T58
 INTERSTATE STANDARD Occupational Safety Standards

System LEATHER

ARTIFICIAL

Fungus Resistance Methods

Occupational safety standards system. Artificial leather.

Methods for determining mold resistance

ISS 13.100
OKP 87 1000
Introduction date 1986-07-01

By the Resolution N 270 of the USSR State Committee on Standards dated February 6, 1985, the introduction date
has been established 01.07.86

The expiration date is lifted under protocol N 5-94 of the Interstate Council for Standardization, Metrology and
Certification (ICS 11-12-94)

REVERSE. September 2003

This standard applies to artificial leather for special clothes, hand protection, technical and domestic purposes, with
and without treatment with fungicides, and establishes methods for determining mushroom resistance.

The essence of the methods consists in maintaining elementary samples of artificial skin contaminated with spores
of certain types of mold fungi, under conditions that are optimal for their development, with subsequent
evaluation of fungus resistance in points.

Methods are also used to determine the effectiveness of the protective action of fungicides.

1. SAMPLING METHOD
1.1. From each roll selected for testing, a point sample 0.3 m long is cut across its entire width.

1.2. For testing from a selected point sample at a distance of at least 50 mm from the edge, twelve elementary
samples of (30 ± 1) x (30 ± 1) mm are cut out.

2. EQUIPMENT, MATERIALS, REACTIVES

2.1. Apply the equipment, materials and reagents according to GOST 9.048-89 for testing .

2.2. For testing artificial leather samples, the following types of fungi are used:

Aspergillus niger van Tieghem; Aspergillus terreus Thom; Chaetomium globosum Kunze; Gladosporium herbarum
BKMF 1686; ex Fr.

3. PREPARATION FOR TESTS

3.1. Preparation for testing is carried out in accordance with GOST 9.048-89 .
3.2. Mold fungi cultures are subcultured from model polyvinyl chloride films (enhanced strains), as indicated in
mandatory Appendix 1.

3.3. For each type of material prepare four Petri dishes.

3.4. To control the vital activity of the mushrooms, nine Petri dishes are prepared for each species according to
para. 2.2.

3.5. Prepare a spore suspension of mixed cultures of mold fungi in distilled water according to GOST 9.048-89 ,
using the types of fungi indicated in paragraph 2.2.

When testing with enhanced strains, a spore suspension of mixed cultures of mold fungi in distilled water according
to GOST 9.048-89 is prepared , using the species of mushrooms that have been sown according to clause 3.2.

3.6. Prepare a suspension of spores of mixed cultures of mold fungi in the environment of čapek-Doksa, diluted
with distilled water in a ratio of 1:15 according to GOST 9.048-89 , using the types of fungi mentioned in paragraph
2.2.

3.7. The medium of čapek-Doksa with agar without sucrose is poured into two Petri dishes in the amount of 20-30
cm and allowed to frost.

3.8. The medium of čapek-doksa with agar and sucrose is poured into two Petri dishes in the amount of 20-30
cm and allowed to cool.

4. TESTING

Mushroom resistance of artificial skin is determined in parallel by two methods.

4.1. Method A

4.1.1. The method establishes whether artificial skin is a breeding ground for the development of fungi.

4.1.2. Elementary samples of artificial leather are cleaned from external contamination with a coarse calf
moistened with ethyl alcohol and placed in Petri dishes.

The tests are carried out in two Petri dishes prepared according to clause 3.7: three elementary samples are placed
in one bowl upwards with the base, and the other three samples are placed upside down with a coating.

4.1.3. Petri dishes with elementary samples are transferred to the box and the surface of the elementary samples is
infected with a suspension of fungi spores prepared according to section 3.5, by uniformly applying it with a spray
bottle, preventing the drops from merging.

4.1.4. Infected elementary samples in Petri dishes are placed in a chamber or a desiccator, at the bottom of which
distilled water is poured, and sealed.

4.1.5. At the same time, control Petri dishes are placed in a chamber or a desiccator, as indicated in Section 3.4.

4.1.6. The tests are carried out at a temperature of (30 ± 2) ° C and a relative air humidity of 95% -100%. Moisture
condensation, forced ventilation and exposure to direct natural or artificial lighting are not allowed in the chamber
or in the desiccator.

4.1.7. The duration of tests of elementary samples of artificial leather for the evaluation of fungus resistance is 28
days; upon infection with reinforced strains, 21 days with an intermediate inspection after 14 days. The duration of
the tests of elementary samples for the evaluation of fungicidal properties is 56 days.
4.1.8. After 5 days, examine the control Petri dishes. If the growth of fungi is not observed on the nutrient medium,
the spores of the fungi used for infection are considered non-viable. The test is repeated on new elementary
samples with a newly prepared suspension from a new batch of mushrooms.

4.1.9. For air inflow, every 7 days, the chamber door or the desiccator lid open for 15 s.

4.2. Method B

4.2.1. The method establishes the presence of artificial skin fungicidal properties and the effect of external
pollution on the fungus resistance.

4.2.2. The elementary samples of artificial leather, not cleared of external contamination, are placed in two Petri
dishes prepared according to section 3.8: three elementary samples are placed in one bowl with the base, and the
other three samples with upward coating are placed.

4.2.3. Infection of elementary samples is carried out, as indicated in clause 4.1.3, with a suspension of fungal spores
prepared according to clause 3.6.

4.2.4. A further procedure for testing - pp.4.1.4; 4.1.6-4.1.9.

4.3. At the end of the test, elementary samples are removed from the chamber or desiccator and inspected with
the naked eye. Inspection is carried out under daylight illumination from the north or with a light source of at least
600 lux. In the absence of the growth of fungi visible to the naked eye, the inspection is continued with an increase
of 56-60 in diffused light at illumination of 2000-3000 lux.

Evaluation of the fungus resistance of artificial skin on the growth of fungi is carried out on a six-point scale in
accordance with table 1.

Table 1
Score The degree of growth of mushrooms
0 When viewed under a microscope, the growth of mold fungi is not visible
one When viewed under a microscope, germinated spores and slightly developed mycelium in the form of non-
branching hyphae are visible.
2 Seen under a microscope, mycelium is seen as branching hyphae, sporulation possible.
3 When viewed with the naked eye, the growth of fungi is barely visible, but clearly visible under a
microscope
four When viewed with the naked eye, the growth of fungi covering less than 25% of the test surface is clearly
visible.
five When viewed with the naked eye, the growth of fungi covering more than 25% of the test surface is clearly
visible.
5. PROCESSING RESULTS
5.1. For the result of the test take the maximum score, which is set for at least two elementary samples, from the
base or coating.

5.2. Evaluation of the mushroom resistance of artificial leather is given in table 2.

table 2
Growth of fungi, score Evaluation of mushroom resistance
Method A Method B
4-5 4-5 Non-resistant
3 3 Satisfactory fungus resistant
1-2 1-3 Mushroom resistant
0 0 Fungicide
5.3. Artificial skin has fungicidal properties, if around the elementary sample on the nutrient medium there is an
inhibitory zone (zone of no growth of fungi) or there is no growth of fungi on the surface and edges of the
elementary samples.

5.4. The test results are recorded in the protocol, the form and the procedure for filling in which are given in the
recommended Appendix 2.

6. SAFETY REQUIREMENTS
6.1. Safety requirements - in accordance with GOST 9.048-89 .

ANNEX 1 (mandatory). CALCULATION OF CUTTED MUSHROOM CULTURES WITH POLYVINYLCHLORIDE FILMS

(STRENGTHENED STRAINS)
ANNEX 1
Mandatory
1. Strengthened cultures of mold fungi are obtained by reseeding them from polyvinyl chloride films without
fungicidal additives.

2. For receiving the strengthened cultures of mushrooms use museum lots of cultures age from 14 to 28 days,
counting from the moment of reseeding.

3. For the cultivation of all cultures of mushrooms using the environment of čapek-Doksa with agar and sucrose.

4. On test tubes prepared for replanting, they indicate the types of repotted mushrooms and set the replanting
date, which will be entered in the register of replanting of fungal cultures.

5. Cultures of fungi for testing are grown in test tubes with a beveled surface of the nutrient medium, on which is
placed a polyvinyl chloride film.

6. Cultures of mushrooms from museum batches are cut onto a polyvinyl chloride film using a bacteriological loop.

7. Test tubes seeded with fungal spores are placed in a thermostat at a temperature of (29 ± 2) ° C and kept in it for
14 days.

8. For the preparation of a suspension of spores of reinforced cultures, cultures of mushrooms from 14 to 28 days
old are used from the time of subculture.

9. A suspension of spores with a concentration of 1-2 million / cm is prepared separately for each type of
fungus. To this end, fungal spores are transferred from a test tube film to a tube containing (15 ± 5) cm
of sterile distilled water.

10. Spores from the tube into the tube transferred by capturing the spores of fungi bacteriological loop. The
number of spores of fungi in suspension is calculated using a Goryaev counting chamber.

APPENDIX 2 (recommended). Mushroom Resistance Test Report

APPENDIX 2
Recommended
NAME OF ORGANIZATION, ENTERPRISES

PROTOCOL N ________
trials on mushroom
resistance
 name of artificial leather

1. The brand of the test material (or its formulation)

2. The number of elementary samples (pcs.)
3. Name of the manufacturer
4. Date of the beginning and end of the test (day, month, year)
5. Designation of the standard by which the test is conducted.
6. Evaluation of elementary sample flexibility (point):
Method A Method B
the first the first
second (from the coating side) second (from the coating side)
the third the third
the fourth the fourth
fifth (from the base) fifth (from the base)
sixth sixth
7. Conclusion on mushroom resistance
Head by laboratory
personal signature full name
Executor
personal signature full name

The text of the document is verified by: the

official publication
Occupational Safety Standards System.
12.4.126-83-GOST 12.4.128-83, GOST 12.4.129-2001,
GOST 12.4.130-83-GOST 12.4.134-83, GOST 12.4.135-84-
GOST 12.4.139-84, GOST 12.4.141-99, GOST 12.4.142-84-
GOST 12.4.149-84, GOST 12.4.150-85-GOST 12.4.155-85,
GOST R 12.4.204-99 (ISO 11933-2-87): Sat Gostov. -
M .: IPK Publishing house of standards, 2003