Effect of pH on Enzyme Activity

(1ML EACH)

WATER
paper turns kind of green (6-7ph)
banana float (bubbles formed) about 2cm of bubbles

HYDROCHLORIC ACID
acid (ph around 1)
banana slightly float (no bubbles formed)
SODIUM HYDROXIDE
base (ph around 12 or 13)
doesn’t float (no bubble formed)

Effect of Temperature Change on Enzyme Activity

ROOM TEMP
bubble formed, banana also float
COLD WATER
bubble formed, banana slightly float
HOT WATER
no bubble formed, banana stays at the bottom

Effect of Substrate Concentration on Enzyme Activity

NO WATER (3% peroxide solution)

thick bubble formed, banana floats
APPRX 2ML WATER (1% concentration)
some bubbles coming out / going up, banana remains at the bottom(didn’t float)
3ML WATER (0.03% / few drop / very low concentration)
very few bubble coming out approximate 1-5 bubbles ? … (almost no bubbles)
Effect of pH on Enzyme Activity
Test Tube 1 WATER
Test Tube 2 HYDROCHLORIC ACID
Test Tube 3 SODIUM HYDROXIDE
Effect of Temperature Change on Enzyme Activity
Test Tube 1 ROOM TEMP WATER
Test Tube 2 COLD WATER
Test Tube 3 HOT WATER
Effect of Substrate Concentration on Enzyme Activity
Test Tube 1 NO WATER (3% peroxide
solution)
Test Tube 2 2ML WATER (1%
concentration)
Test Tube 3 3ML WATER (0.03% / few
drop / very low concentration
Paper turns kind of green (6-7ph)
The Banana float and there is also about 2cm of bubbles
formed
Acid (pH around 1)
The Banana slightly Float but there’s no bubble formed
Base (pH around 12 - 13)
The banana doesn’t float and there is also no bubble
formed
Bubbles formed and the banana also floats
Bubbles formed and the banana slightly float
No bubbles form and the banana remain at the bottom
of the test tube
Thick bubble formed and the banana also floats
Some bubbles coming out / going up but banana
remains at the bottom of the test tube
very few bubbles coming out approximately 1-5
bubbles? … (almost no bubbles)