GOVERNMENT COLLEGE UNIVERSITY Roll No.

_________________
FAISALABAD
Question Paper: (Objective Type) Discipline: Medical Laboratory Technology
Examination: Mid Term Semester: 4th
Course Title: Histopathology & Histotechnology Couse Code: MLT-408
Time Allowed: 20 minutes Marks: 10
Question # 1: Encircle the best option. (Cutting and over writing is not allowed)

1. All of these factors of characteristics for fixation except

a.) Arrests autolysis b.) Activates bacterial decomposition

c.) Minimizes loss of cytoplasmic components d.) Stabilizes tissue for further treatment
2. When preparing a tissue for light microscopy which method precedes clearing the specimen with organic solvent

a.) Fixation b.) clearing

c.) Embedding d.) staining
3. How can you achieve better resolution in microscopy?

a.) Using paraffin-embedded sections b.) Using thicker resin-embedded sections

c.) Using formaldehyde-embedded sections d.) Using a mixture of paraffin and resin
4. very expensive but most ideal and reliable method to check the extent of decalcification is?

a.) X Rays b.) Chemical

c.) Physical d.) Chelation
5. Slow dehydration of tissue in increasing grades of alcohol is advised to avoid

a.) Penetration b.) Shrinkage

c.) Staining d.) Autolysis
6. In _____ microtome knife is moved horizontally against fixed block

a.) Rotary b.) Rocking

c.) Sliding d.) Rotary Rocking
7. Trimming is usually done at thickness

a.) 2-4 µm b.) 5 µm

c.) 20-30 µm d.) 10 µm
8. If sections are too thick what will be probable cause

a.) Wrong micrometer setting b.) Sectioning at recommended speed

c.) Good processing d.) New microtome blade/knife
9. The most used basic dye is

a.) Eosin b.) Methylene Blue

c.) Hematoxylin d.) Toluidine
10. Time required for water bath flattening of ribbon floating of sections after microtomy is

a.) 30 minutes b.) 30 seconds

c.) 5 minutes d.) 5 seconds

GOVERNMENT COLLEGE UNIVERSITY Roll No. _________________

FAISALABAD
Question Paper: (Subjective Type) Discipline: Medical Laboratory Technology
Examination: Mid Term Semester: 4th
Course Title: Histopathology & Histotechnology Couse Code: MLT-408
Time Allowed: 30 minutes Marks: 8
(Attempt all questions) Marks (02 each)

Question # 2: Name steps involved in tissue processing?

Question # 3: What are advantages and disadvantages of Frozen sections cut by microtomes?
Question # 4: If we have different types of specimens after microtomy, how many specimens we can float at
the same time in a water bath and why?
Question # 5: A section is 4µm thick and cohesive, other section is 2µm thick and severely disrupted, which
section will give better results in microscopy and why?

11. All of these factors of characteristics for fixation except

a.) Arrests autolysis b.) Activates bacterial decomposition

c.) Minimizes loss of cytoplasmic components d.) Stabilizes tissue for further treatment
12. When preparing a tissue for light microscopy which method precedes clearing the specimen with organic solvent

a.) Fixation b.) clearing

c.) Embedding d.) staining
13. How can you achieve better resolution in microscopy ?

a.) Using paraffin-embedded sections b.) Using thicker resin-embedded sections

c.) Using formaldehyde-embedded sections d.) Using a mixture of paraffin and resin
14. 1. In _____ microtome knife is moved horizontally against fixed block

a.) Sliding b.)

c.) d.)
2. Slow dehydration of tissue in increasing grades of alcohol is advocated to avoid

a.) Penetration b.) Shrinkage

c.) Staining d.) Autolysis
3. very expensive but most ideal and reliable method to check the extent of decalcification is?

a.) X Ray b.) Chemical

c.) Physical d.) Chelation
4. Trimming is usually done at thickness

a.) 2-4 µm b.) 5 µm

c.) 20-30 µm d.) 10 µm
5. If sections are too thick what will be probable cause

a.) Wrong micrometer setting b.) Sectioning at recommended speed

c.) Good processing d.) New microtome blade/knife
6. The most commonly used basic dye is
 a.) Eosin b.) Toluidine
c.) Hematoxylin d.) Methylene blue
7. Time required for water bath flattening of ribbon floating of sections after microtomy is

a.) 30 minutes b.) 30 seconds

c.) 5 minutes d.) 5 seconds