LANES

Electrophoresis Transfer Immunodetection

11 15

Electrophoresis and Western Blotting Layout 22 16

EXPERIMENT

USER DATE
33 17
MW STANDARD
44 18
GEL TYPE % BUFFER TIME VOLTAGE

Remove the bottom sealing tape before you forget! 55 19

MEMBRANE TYPE

66 20
TRANSFER CONDITIONS AMPS VOLTS TIME

BLOCKING BUFFER TYPE VOLUME TIME

77 21
1º AB 1ST TARGET DILUTION TIME

1º AB 2ND TARGET DILUTION TIME 88 22

1º AB 3RD TARGET DILUTION TIME
99 23
1º AB WASH BUFFER VOLUME TIME CYCLES

2º AB SPECIES AND CONJUGATE DILUTION TIME 10 24

2º AB SPECIES AND CONJUGATE DILUTION TIME

2º AB SPECIES AND CONJUGATE DILUTION TIME

11 25
2º AB WASH BUFFER VOLUME TIME CYCLES
12 26
ECL SUBSTRATE TYPE VOLUME

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NOTES 13 for more information.

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