Worksheet

Observing a Hay Infusion/ Pond water observation

What is a Hay infusion?
A hay infusion is a process of using pond water and hay in order to safely multiply microorganisms, so that they
can be studied under a microscope.

Instructions:

1. Perform a wet mount using a drop of water supplied by your teacher. Use the proper placement of the coverslip and
test tube in performing the wet mount.

2. Place the wet mount in the microscope. Focus the microscope using the LPO. After that, use the HPO to zoom in. Scan
the entirety of the wet mount so that you won’t miss any of the organisms there.

3.Try to find as many organisms as you can. Draw these on organisms on your notebook. You can also take pictures and
post them on your social media with the hashtag #microscopy. Make sure to tag your teacher.

Organisms that I saw.

1. Name: ______________ 2. Name: ______________ 3. Name: ______________ 4. Name: ______________

5. Name: ______________ 6. Name: ______________ 7. Name: ______________ 8. Name: ______________

3. What do you think are the organisms that you see. Use this Picture guide to find out some of the common
microorganisms

Euglena Amoeba Paramecium

Yeast Mosquito Larvae Pond water Algae

 Rotifer Blood worms Daphnia

4. If you find anything that Is not on the picture guide, draw them and research about them. Tell your teacher what you
found out about them tomorrow.

Questions:

1. What can you say about the organisms that you saw?

2. Did you see any moving organisms?

3. Did you see more or less organisms as time passed?

4. Did the movement of the organisms decrease or increase as time went on?

5.Can you drink this water? Why or why not?