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MLS 038 (Principles of Medical Laboratory

Science and Practice 2)
STUDENT ACTIVITY SHEET BS MEDICAL TECHNOLOGY / FIRST YEAR
Session # 2 & 3

LESSON TITLE: PRE-ANALYSIS Materials:

LEARNING OUTCOMES: Book, pen and notebook, index card/class list,

evacuated tubes
Upon completion of this lesson, the MedTech student can:

1. Define key terms in this chapter References:

2. Identify patient variables including physical activity,
circadian rhythm, diet, medications, age, sex, etc. that can
affect test results
3. Explain the importance of proper collection and handling
techniques of samples
My Library
1. Bishop, M. L., Fody, E. P. & Schoeff, L. E.
(2010). Clinical Chemistry: Techniques,
Principles, Correlations, 8th ed. Philadelphia, PA:
Lippincott Williams & Wilkins.
2. Mcpherson, R.A. & Pincus, M.R. (2017).
Henry’s Clinical Diagnosis and Management by
Laboratory Methods, 23rd ed. Philadelphia:
Elsevier Inc.
3. Strasinger, SK and Di Lorenzo, MS (2011) The
phlebotomy Textbook (3rd ed) Philadelphia: FA
Davis.

SUBJECT ORIENTATION (10 minutes)

Students are asked to read the lesson ahead of time. Main reference of the lesson is found on Chapter 3: Pre-analysis,
20-32 of reference book 2.

MAIN LESSON (50 minutes)

Lesson Outline:
A. Definition of Terms
B. Pre-collection Variables
C. Specimen Collection
D. Specimen Transport and Processing

Lesson Notes:
A. Definition of Terms

1. Anticoagulant - tube additive that prevents blood clotting

2. Basal State - refers to the resting metabolic state of the body early in the morning after fasting for a minimum of
12 hours.
3. Clot activator - tube additive that accelerates clotting
4. Diurnal variation - normal daily fluctuations in body chemistry related to hormonal cycles, sleep-wake cycles, and
other regular patterns of change
5. Hemolysis - rupture or destruction of Red Blood Cells
6. Icteric - used to describe a serum or plasma sample that is bright to dark yellow due to excess bilirubin
7. Lipemia - a turbidity of the serum or plasma sample caused by increased lipids, specifically the lipoprotein
Chylomicrons.

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