CHAPTER 2 MATERIAL AND METHODS

2.1 Experimental work-

A) Instrumentation

Table 2.1: List of instruments and equipments

Instrument Source

HPLC system Shimadzu SPD-M20A, Prominence Diode

array detector, Software- LC-Solution
pH meter Equip-Tronics pH meter, EQ-610, Equip
Sonicator UCB40 spectra Lab, Mumbai
UV-Vis spectrophotometer V630 Jasco, Japan
Electronic balance CB-50 Contech, Navi Mumbai
Distillation Unit Lab Sil Instrument, Bangalore
Hot air oven 250BSS, Pathak, Mumbai
Constant temperature water Intex, FRNW-326
bath
B) Chemical and reagent

1) Acetonitrile (HPLC grade), Research Lab., Mumbai, India

2) Methanol (HPLC grade), Research Lab., Mumbai, India

3) Water (HPLC grade), double distilled, Lab sil Instrument, Bangalore, India

4) Ammonium Acetate (AR grade), Research Lab., Mumbai, India

5) Hydrochloric acid (AR grade), Qualigens fine chemicals, Mahape, India

6) Sodium Hydroxide(AR grade), Research Lab., Mumbai, India

7) Hydrogen peroxide (LR) S.D. Fine-Chem Ltd., Mumbai, India

8) Glacial acetic acid (AR grade), Research Lab., Mumbai, India

C) Consumables

Column - ACE (150 x 4.6 mm, 5 µm)

Filter paper - Pall India PVT LTD (0.22 µm)

Sinhagad Institute of Pharmacy Narhe Pune - 41 34