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Connie R. Mahon
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Textbook of Diagnostic Microbiology
SIXTH EDITION
2
Table of Contents
Cover image
Title Page
Copyright
Dedication
Reviewers
Contributors
Preface
Organization
Pedagogic Features
Acknowledgments
Part I Introduction to Clinical Microbiology
Classification/Taxonomy
Bacterial Morphology
3
Bacterial Biochemistry and Metabolism
Bacterial Genetics
Bibliography
Role of the Microbial Biota in the Host Defense Against Infectious Disease
Bibliography
Outbreak Investigation
Education
Bibliography
Microbiology Safety
Bibliography
4
Chapter 5 Performance Improvement in the Microbiology Laboratory
General Guidelines for Establishing Quality Control
Performance Improvement
Test Validation
Bibliography
Culture Workup
Bibliography
Stains
Microscopes
Bibliography
5
Direct Examination in Gram-Positive Bacillary Infections
Bibliography
Miscellaneous Tests
Bibliography
Antigen Detection
6
Bibliography
Nanomedicine
Bibliography
β-Lactamase Inhibitors
Bibliography
Time-Kill Assays
Synergy Tests
7
Serum Bactericidal Test
Bibliography
Chapter 14 Staphylococci
General Characteristics
Laboratory Diagnosis
Antimicrobial Susceptibility
Bibliography
Laboratory Diagnosis
Bibliography
Bibliography
Bibliography
8
Negative Bacilli
Chapter Outline
Objectives
Key Terms
Haemophilus
HACEK Group
Capnocytophaga
Pasteurella
Brucella
Francisella
Bibliography
Chapter Outline
Objectives
Key Terms
Legionella
Laboratory Diagnosis
Bordetella
Bibliography
Chapter 19 Enterobacteriaceae
General Characteristics
Bibliography
Aeromonas
Bibliography
9
Chapter 21 Nonfermenting and Miscellaneous Gram-Negative Bacilli
General Characteristics of Nonfermenters
Bibliography
Bibliography
Borreliae
Treponemes
Bibliography
Bibliography
Clinical Infections
Laboratory Diagnosis
Antimicrobial Susceptibility
Bibliography
10
Chapter 26 Mycobacterium tuberculosis and Nontuberculous Mycobacteria
General Characteristics
Mycobacterium leprae
Bibliography
Taxonomy
Mycoses
Antifungal Susceptibility
Bibliography
Bibliography
Hepatitis Viruses
11
Prions
Antiviral Therapy
Bibliography
Biosafety
Biosafety Levels
Agents of Bioterror
Concluding Thoughts
Forensic Microbiology
Bibliography
Chapter 31 Biofilms
Microbial Biofilms Defined
Architecture of Biofilms
Biofilm Properties
Mechanisms of Pathogenicity
Detection of Biofilms
Potential Interventions
Bibliography
12
Virulence Factors of Pathogenic Organisms
References
Viral Infections
Parasitic Infections
Laboratory Diagnosis
Bibliography
Anatomic Considerations
Bibliography
Laboratory Diagnosis
Bibliography
13
Chapter 36 Bacteremia and Sepsis
General Concepts Related to Bacteremic Infections
Epidemiology
Causes
Pathogenesis
Laboratory Diagnosis
Treatment
Prevention
Bibliography
Laboratory Diagnosis
Microbial Detection
Interpretation of Results
Susceptibility Reporting
Bibliography
Cervicitis
Vulvovaginitis
Bibliography
14
Health Care–Associated Infections
Ventilator-Associated Pneumonia
Malignancy
Complement Deficiency
Burns
Organ Transplantation
Postsplenectomy Sepsis
Cystic Fibrosis
Diabetes
Aging
Bibliography
Emerging Zoonoses
Bibliography
Bibliography
15
Appendix A Selected Bacteriologic Culture Media
Acetate Agar
Cefsulodin-Irgasan-Novobiocin
Cetrimide Agar
Chocolate Agar
CHROMagars
16
Dilute Gelatin Medium (0.4%)
Esculin Agar
Gram-Negative Broth
Hippurate Broth
Lim Broth
Löwenstein-Jensen Medium
MacConkey Agar
Malonate Broth
Mueller-Hinton Agar
Nutrient Agar
17
Phenylalanine Deaminase Agar
PPLO Agar
Regan-Lowe Medium
Salmonella-Shigella Agar
Schaedler Agar
Selenite Broth
Streptococcus-Selective Agar
Tetrathionate Broth
Tinsdale Agar
Tryptophan Broth, 1%
Vaginalis Agar
Xylose-Lysine-Desoxycholate Agar
Bibliography
Fungal Stains
Bibliography
18
Glossary
A
Index
19
Copyright
Elsevier Saunders
3251 Riverport Lane
St. Louis, Missouri 63043
This book and the individual contributions contained in it are protected under
copyright by the Publisher (other than as may be noted herein).
Notices
Practitioners and researchers must always rely on their own experience and
knowledge in evaluating and using any information, methods, compounds or
experiments described herein. Because of rapid advances in the medical
sciences, in particular, independent verification of diagnoses and drug dosages
should be made. To the fullest extent of the law, no responsibility is assumed by
Elsevier, authors, editors or contributors for any injury and/or damage to
persons or property as a matter of products liability, negligence or otherwise, or
from any use or operation of any methods, products, instructions, or ideas
contained in the material herein.
20
Names: Mahon, Connie R., editor. | Lehman, Donald C., editor.
Title: Textbook of diagnostic microbiology / [edited by] Connie R. Mahon,
Donald C. Lehman.
Description: Sixth edition. | St. Louis, Missouri : Elsevier Saunders, [2019] |
Includes bibliographical references and index.
Identifiers: LCCN 2017050818 (print) | LCCN 2017051723 (ebook) | ISBN
9780323482127 (ebook) | ISBN 9780323482189
Subjects: | MESH: Microbiological Techniques | Communicable Diseases—
diagnosis | Bacterial Infections—diagnosis | Virus Diseases—diagnosis |
Mycoses—diagnosis
Classification: LCC QR67 (ebook) | LCC QR67 (print) | NLM QW 25 | DDC
616.9/041—dc23
LC record available at https://lccn.loc.gov/2017050818
21
Dedication
To my husband Dan for his love and continued support and understanding;
my son Sean who inspires me; my daughter Kathleen, for showing me courage;
and my granddaughters Kelly Amelia, Natalie Page, and Madeline Belle, who
have given us so much pleasure.
CRM
To my wife Terri, for her constant support and encouragement, and whose love
makes me realize anything is possible, and my grandchildren Shane, Athena,
Jordan, and Vincent, who keep me young at heart.
DCL
22
Reviewers
Keri Brophy-Martinez MHA/ED, MT(ASCP)
Department Chair/Professor
Medical Laboratory Technology
Austin Community College
Austin, Texas
23
Assistant Professor of Biology
University of Saint Francis
Crown Point, Indiana
24
Clinical Laboratory Science
College of Southern Nevada (Full Time)
Diagnostic Center of Medicine (Part Time)
Las Vegas, Nevada
25
Contributors
Yousif Barzani MD, MLS(ASCP)CM
Assistant Professor
Department of Integrated Health Sciences
School of Medicine and Health Sciences
The George Washington University
Washington, DC
Nina Clark MD
Professor
Department of Medicine
Division of Infectious Diseases
Director, Transplant Infectious Diseases
Loyola University Medical Center
Maywood, Illinois
James L. Cook MD
Clinical Professor of Medicine
Division of Infectious Diseases, Department of Medicine
Loyola University Medical Center
Maywood, Illinois
Staff Physician and Research Scientist
Infectious Diseases Section
Edward Hines, Jr. VA Hospital
Hines, Illinois
26
George Washington University
Washington, DC
Adjunct Instructor
CAHS School of Social Work
Cincinnati, Ohio
27
Newark, Delaware
28
Silver Spring, Maryland
Susan M. Pacheco MD
Physician
Medicine
Edward Hines, Jr. VA Hospital
Hines, Illinois
Assistant Professor
Department of Medicine
Loyola University Medical Center
Maywood, Illinois
29
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