Measurement uncertainty using the Nordtest approach

Acrylamide determination in crisp bread

Analysis of certified reference materials

Crisp bread Potato chips

C_ref = 1179 μg/kg C_ref = 860 μg/kg
U (k=2) = 68 μg/kg U (k=2) = 42 μg/kg

Days C (µg/kg) Days C (µg/kg)

1/5/2008 1172 4/3/2008 845
3/6/2008 1186 4/3/2008 832
4/3/2008 1153 4/3/2008 802
1/8/2009 1151 4/27/2008 829
3/18/2009 1181 4/27/2008 851
4/3/2009 1147 4/27/2008 834
4/11/2009 1097
4/16/2009 1102
4/25/2009 1162 Mean: 832 μg/kg
8/3/2009 1138 Std Dev: 17 μg/kg
8/28/2009 1122
11/27/2009 1191

Mean: 1150 μg/kg

Std Dev: 31 μg/kg
Measurement uncertainty using the Nordtest approach
Acrylamide determination in crisp bread
Measurement result value: 998 μg/kg
uncertainty is estimated in relative quantities

Reproducibility, u(Rw)
From repeated analysis of one of the CRMs
u(Rw) = s_Rw = 31 μg/kg
u(Rw)_rel = 2.70 %

Bias, u(bias)
From measurements with 2 different CRMs

Ref value U (k=2) uc Lab result uc_rel biasi bias_rel

μg/kg μg/kg μg/kg μg/kg % μg/kg %
1179 68 34 1150 2.88 -29 -2.45
860 42 21 832 2.44 -28 -3.24

u(Cref)_rel= 2.67 %
RMSbias_rel= 2.87 %
u(bias)_rel = 3.92 %

Result:
uc_rel = 4.8 %
uc = 48 μg/kg
U_rel (k=2) = 9.5 %
U (k=2) = 95 μg/kg