Operation Manual SM ECO ABC - Bioair

Australian Biosafety Cabinet
Class II Biological Safety Cabinets
OPERATING MANUAL
PART 1: USER
PART 2: INSTALLATION AND MAINTENANCE
SafeMate Eco+ ABC
ORIGINAL INSTRUCTION
80.021.210 Revision 00 April 2021
BioAir S.p.A.
Head Offices and Manufacturing Plant: Via Lombardia, 12 - 27010 Siziano (PV) Italy
+39 0382 6672.1
www.bioair.it - info@bioair.it
BioAir has a Quality System certified in compliance with EN ISO 9001
80.021.210 R00 - April 2021
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SafeMate Eco ABC Operating Manual
CONTENTS
GENERAL INFORMATION 7
FOREWORD TO MANUAL 7
MARKING 8
WHAT IS SUPPLIED INSIDE 8
MEANING OF SYMBOLS 9
GLOSSARY 10
WARRANTY AND LIABILITY 10
DEFINITION OF IMPROPER USE 10
CABINET DEFINITION 10
INTENDED USE 11
NOT ALLOWED USE 11
DECLARATION OF CONFORMITY 12
APPROVALS AND CERTIFICATIONS 12
CONTACTS 13
PART 1: USER 15
1. ASSESSMENT OF RISKS 17
2. INSTALLATION 24
2.1 CHOICE OF LOCATION 24
2.2 FLOOR STAND 25
2.2.1 Restrictions on adjustable stand use 25
2.3 CONNECTING TO THE MAINS 26
2.3.1 Mains supply cord 27
2.3.2 Mains socket 27
2.3.3 Appliance mains inlet and main fuses 28
2.4 CONNECTION OF GAS LINES (OPTIONAL) 28
2.5 EXHAUST AIR TREATMENT 28
2.6 FINAL PROCEDURES 29
3. TECHNICAL DATA 30
3.1 POWER SUPPLY 30
3.2 REFERENCE STANDARDS 30
3.3 DECLARATIONS AND APPROVALS 30
3.4 USE ENVIRONMENTAL CONDITIONS 31
3.5 TRANSPORT AND STORAGE CONDITIONS 31
3.6 WEIGHT AND DIMENSIONS 31
3.7 PERFORMANCES 32
3.8 MATERIALS 32
3.8.1 METAL PARTS 32
3.8.2 GLASS PARTS 33
3.8.3 FILTERS 33
3.8.4 LIGHTING (2) 33
3.8.5 UV-C LAMP 34
3.9 OUTLET 34
3.9.1 SERVICE SOCKETS 34
3.9.2 CONNECTOR VFC 34
3.10 OPTIONAL ACCESSORIES FEATURES 34
3.10.1 FLOOR STAND 34
3.10.2 INERT FLUIDS UTILITY 35
3.10.3 FLAMMABLE GAS UTILITY 35
3.10.4 LED LIGHTING 35
3.11 ORDINARY OPTIONALS 36
4. OPERATING PRINCIPLE 37
5. OPERATING INSTRUCTIONS 38
5.1 CABINET DESCRIPTION 38
5.1.1 Lighting 39
5.1.2 Service sockets 39
5.1.3 Front window 40
5.1.3.1 FRONT WINDOW MOVEMENT JAMMING 40
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5.1.4 UV-C germicidal lamp 41

5.1.4.1 PROTECTION AGAINST UV-C RADIATIONS 42
5.1.5 Gases utilities (optional) 43
5.1.5.1 LINE FOR INERT GASES, VACUUM AND COMPRESSED AIR 44
5.1.5.2 FLAMMABLE GAS LINE 44
5.1.6 Working chamber 45
5.1.6.1 WORK SURFACE 45
5.1.6.2 SAFE WORKING AREA 45
5.1.6.3 INTAKE GRILLES 46
5.1.7 CONTROL PANEL 47
5.1.7.1 Quarter-turn locks 47
5.1.7.2 Start/stop switch 47
5.1.7.3 Alarm pilot light 47
5.1.7.4 Service outputs and inputs 47
5.1.7.5 Key pad 48
5.1.7.6 Display 48
5.1.7.7 Normal keys function 48
5.1.7.8 Window sash keys 48
5.1.7.9 Advanced keys function 49
5.2 OPERATING PROCEDURES 50
5.2.1 MODE 1 ACCESS 51
5.2.1.1 Lighting ON/OFF 51
5.2.1.2 Sockets ON/OFF 51
5.2.1.3 Solenoid GAS valve ON/OFF 51
5.2.1.4 Run hours meter 51
5.2.1.5 ECO MODE 52
5.2.1.6 IRREGULARITIES AND ALARMS 53
5.2.2 MODE 2 ACCESS 54
5.2.2.1 Lighting ON/OFF 54
5.2.2.2 Sockets ON/OFF 54
5.2.2.3 Language selection 54
5.2.2.4 Date and time setting 54
5.2.2.5 Activation and programming of UV-C cycle 55
5.2.3 SHUTDOWN 55
5.2.3.1 Operational shutdown 55
5.2.3.2 Disconnection from mains 55
6. GENERAL RULES – GUIDELINES 56

6.1 FEATURES OF LOCATION: 56
6.2 WORKING POSTURE: 56
6.3 WORKING PROCEDURES: 56
7. CLEANSING AND DECONTAMINATION 57
7.1 CLEANSING 57
7.1.1 Painted surfaces 57
7.1.2 Stainless steel surfaces 57
7.1.3 Glass surfaces 57
7.1.3.1 Complete cleaning of the front window 58
7.1.4 Equalizer fabric of LAF filter 59
7.2 DAILY DECONTAMINATION 60
7.3 BIOLOGICAL DECONTAMINATION 60
7.4 LAMPS REPLACEMENT 60
7.4.1 UV LAMP 61
7.4.2 FLUORESCENT LAMPS 61
7.4.3 IN CASE OF BREAKAGE OF LAMPS 62
7.4.4 DISPOSAL OF LAMPS 62
PART 2: INSTALLATION AND MAINTENANCE 63
8. INSTALLATION 65
8.1 HANDLING AND UNPACKING 66
8.2 POSITIONING 66
8.3 INSTALLATION OF OPTIONAL EQUIPMENT 67
8.3.1 FLOOR STAND 67
8.3.1.1 Stand assembling 67
8.3.1.2 Placing of the cabinet on the stand 67
8.3.2 ASSEMBLING OF GASES AND FLUIDS TAPS 69
8.3.2.1 Testing of gas circuits tightness 69
8.3.3 Ducting of exhausted air flow 70
8.3.4 VFC output (auxiliary contact) 71
8.3.5 Alarm mute inlet 71
8.3.6 Data output port 71
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8.4 DISPOSAL OF THE PACKAGING 71

8.5 CONNECTION TO THE MAINS 72
PROTECTION DEVICE FOR THE ELECTRIC LINE 72
SUPPLY BY UNINTERRUPTIBLE POWER SUPPLY (UPS) 72
8.5.1 Mains socket 73
8.5.2 Mains supply cord 73
8.6 CONNECTION TO THE GAS LINES (OPTIONAL) 74
8.6.1 Vacuum/Inert gases line 74
8.6.2 Flammable gas line 74
8.7 PERFORMANCE TEST 75
ADVISED ADDITIONAL INSTRUMENTS 75
8.8 FILTERS INTEGRITY TEST (DOP TEST) 75
8.8.1 Exhaust and LAF filters testing 75
8.9 AIRFLOWS TEST 76
8.9.1 INFLOW air: 77
8.9.2 DOWNFLOW air (LAF): 78
8.9.3 CONTAINMENT AT THE FRONT APERTURE: 79
8.9.4 WORK ZONE INTEGRITY: 79
8.9.5 Smoke pattern test: 79
8.9.6 Working area air cleanliness test 79
8.9.7 Check of the alarm thresholds: 80
8.9.8 Manual control of the fan: 81
9. BIOLOGICAL DECONTAMINATION 82
9.1 DECONTAMINATION BY MEANS OF H2O2 83
9.1.1 OPERATING PRINCIPLE 83
9.1.2 REQUIRED EQUIPMENT 83
9.1.2.1 MICRONEBULIZER 83
9.1.2.2 CONTAINMENT BAG 83
9.1.2.3 RICIRCULATION TUBE 83
9.1.2.4 DECONTAMINATING SOLUTION 84
9.1.2.5 PROCESS CHEMICAL INDICATORS 84
9.1.2.6 VARIE 84
9.2 DECONTAMINATION PROCEDURE 85
9.2.1 PREPARATION OF THE CABINET 85
9.2.2 ACTIVATION OF THE DECONTAMINATION CYCLE 87
9.2.3 REMOVAL OF THE DECONTAMINATION SYSTEM 89
9.2.3.1 H2O2 bottle extraction 89
9.2.4 Automatic cycle for alternative fumigation 90
9.2.4.1 PROCEDURE 90
10. SERVICE AND MAINTENANCE 91

10.1.1 ROUTINE CHECKS 91
10.1.2 FILTERS REPLACEMENT 92
10.1.2.1 DISPOSAL OF FILTERS 92
10.1.3 REPLACEMENT OF VARIOUS PARTS 92
10.1.4 CALIBRATION AND PC BOARD SETTING 92
10.2 TROUBLE SHOOTING 93
10.3 SPARE PARTS LIST 94
10.3.1 Common replacements 94
10.3.2 Critical replacements 94
10.3.3 Full list 95
11. WIRING DIAGRAM 95
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GENERAL INFORMATION
FOREWORD TO MANUAL
Original instructions.
In case of a disagreement between the translation and the original version of this manual or a notice or
disclaimer, the original English version will prevail.
This manual is intended to provide the USER with instructions for use and to provide the SERVICE with
installation and maintenance instructions, for the following cabinet family:
Model: +
SafeMate Eco ABC
Size: 0.9 0.9 0.9 0.9
Code: LDM3802 LDM4802 LDM5802 LDM6802
The use of this equipment is limited by what is described in the paragraphs CABINET DEFINITION,
INTENDED USE and DEFINITION OF IMPROPER USE.
WARNING!
The operating manual is an integral part of the machine and it refers to the above cabinet
codes only.
Special versions of this cabinet with different code could be partially covered by this
manual, but they could also need additional instruction to add or substitute parts of this
manual.
In order to make the guide as complete and comprehensible as possible, the utmost care
has been taken in the collection and verification of the documentation contained in this
manual, but it is not designed to act as your procedure manual or act as a substitute for
validation of your own procedures.
The cabinet code is printed on the data label (see MARKING).
Unless otherwise specified, the information contained in this manual is applicable to all
codes listed above.
 Read the manual before starting ANY ACTIVITY involving the machine, including its
handling, installation and maintenance.
 The instructions given in this Manual shall be carefully read and understood before
switching this equipment on before you operate it, in order to become familiar with its
operation and function, and to avoid an IMPROPER USE.
 This manual should always be available in the operating area of the cabinet, in order to be
quickly consulted for use and service (routine checks and maintenance, and must
accompany it from the time of its manufacture until its dismantling, in the event of
relocation, sale, etc.
 In case of special cabinets derived from this model, consult the dedicated manual or the
supplementary manual referring to the code of the special cabinet.
For a better consultation, the operating manual is divided into the following sections:
 Part 1: containing the use instructions for the OPERATOR.
 Part 2: containing installation and maintenance instructions, intended solely for suitably trained
TECHNICAL personnel authorized to work on the cabinet.
BioAir reserves the right to modify or update the content at any time without prior notice.
Nothing contained in this manual can be considered as a warranty, either expressed or implied,
including, not in a restrictive way, the suitability warranty for any special purpose.
Nothing contained in this manual can be interpreted as a modification or confirmation of the terms of
any purchase contract.
ALL RIGHTS ARE RESERVED ACCORDING TO THE INTERNATIONAL COPYRIGHT CONVENTIONS,

the reproduction of any part of this manual, in any form, is forbidden without the prior written
authorization of BioAir S.p.A. (hereinafter referred to as BioAir).
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MARKING
On the right side of the control box of every apparatus (see 5.1 - Pic. 15 – item 4) a label is placed with
the following important information as shown in the example below (Pic. 1):
 Logo
 Name of manufacturer
 Address of manufacturer
 CE Mark (for European Market only)
 Model
 Code
 Voltage, Frequency, Power
 Warnings (3)
 Serial number
 Year of manufacturing
 Standard conformity (1)
 Competent Body Mark (2)
 Factory use code (4)
Pic. 1
Do not remove this label.
For any enquiry relevant to service or spares parts, please always communicate the data on this label
relating to CODE, MODEL and SERIAL NUMBER.
WHAT IS SUPPLIED INSIDE

The following parts are provided inside packaging:
ON THE TOP INSIDE WORK ZONE
Intake front grille.
Power cord Work surface.
ON THE SIDE OF CABINET

IN PLASTIC BAG IN CARDBOARD
BOX
BIOHAZARD Inert gas tap

Power cord fixer Switch keys adhesive label install kit
80080158
Flammable gas
This manual Short instruction tap install kit
80.021.210 Shelves extractor Control panel keys adhesive label 80080159
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MEANING OF SYMBOLS
GENERAL WARNING
CAREFULLY READ THE MANUAL BEFORE USING
ELECTRIC SCHOCK HAZARD WARNING
BIOLOGICAL HAZARD WARNING
PINCH HAZARD WARNING
UV RADIATIONS HAZARD WARNING
DO NOT USE products containing chlorine
DO NOT USE RADIOACTIVE MATERIAL
DO NOT USE CYTOTOXIC / CHEMICAL MATERIAL
DO NOT USE IN RESIDENTIAL AREAS
DO NOT USE IN EXPLOSIVE RISK AREAS
GENERAL SIGN OF MANDATORY PROVISIONS TO BE IMPLEMENTED
DISCONENCT THE CABINET FROM THE MAINS (by means of power cord plug)
SEE SPECIFIC DOCMENTATION
MAIN GROUND TERMINAL BLOCK
Separated collection for electric and electronic equipment.

According to 2012/19/EU DIRECTIVE (WEEE)
User information
1
NOT FOR USER, Service information only

2
OPERATION THAT REQUIRES TWO PEOPLE
IMPORTANT NOTE
OPERATIONS ALLOWED ONLY TO PERSONNEL TRAINED AND AUTHORIZED BY BioAir
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GLOSSARY
Apf: Aperture protection factor.
BSC: Biological Safety Cabinet.
DOP: Dispersed Oil Particle.
EMC: Electromagnetic Compatibility.
EMI: Electromagnetic Interference.
Exh: Exhaust air flow.
HEPA: High Efficiency Particulate Air.
LAF: Laminar Air Flow.
MPPS: Most Penetrating Particle Size.
PPD: Personal Protection Device
SDS: Safety Data Sheet
SOP: Standard Operating Procedure
TLV: Threshold Limit value
UPS: Uninterruptible Power Supply
UV-C: Ultraviolet radiation (range C - germicidal).
VFC: Voltage Free Contact.
WARRANTY AND LIABILITY

Warranty and liability claims in the event of injury and property damage will not be accepted if these
can be attributed to one or more of the following causes:
 IMPROPER USE of the cabinet

 Inexpert installation, start-up, operation, servicing and repair work of the cabinet
 Combinations with accessories (e.g. FLOOR STAND) not authorised by BioAir
 Installation or use despite the presence of damage or defects
 Use of the cabinet with defective safety equipment or not correctly fitted or not in proper working
order
 Use of inadequate techniques in the workmanship
 Non-observance of the safety instructions
 Non-observance of the installation and maintenance instructions
 Unauthorised structural modifications
 Force majeure
 Handling with unsuitable equipment and means
 Defects in supply lines
 Missing or defective earth connection
DEFINITION OF IMPROPER USE

Any installation and use of this equipment, other than what is described in this manual, is to be
considered improper use.
Use of this equipment for works and in manner not provided for in this manual and going against with
its DEFINITION and INTENDED USE is to be considered IMPROPER USE.
CABINET DEFINITION
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The definition of the cabinet SafeMate Eco ABC is the following:
CLASS II BIOLOGICAL SAFETY CABINET (BSC)
FOR PERSONNEL, ENVIRONMENT AND PRODUCT PROTECTION as per AS 2252.2-2009
NOT DESIGNED FOR USE WITH FLAMMABLE, EXPLOSIVE OR HIGHLY VOLATILE LIQUIDS, CYTOTOXIC
DRUGS OR TOXIC COMPOUNDS.
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INTENDED USE
The purpose of the cabinet is to provide protection from hazardous biological agents for personnel and
the environment and also to protect material used in the cabinet from exogenous contamination.
This cabinet (hereinafter also named BSC) is designed to reach the following targets during the
handling of biological agents and hazardous or potentially hazardous microorganisms:
 reducing of the risk to the operator
 protection of the environment
 protection of the product against environmental and cross contamination risk
NOTE: the categories of etiological agents are defined by the statutory legislation in your country.
Reference to the listed classes of microbial species in order to assess which ones can be handled in this
BSC.
NOT ALLOWED USE

WARNING!
This BSC is not suitable for handling with chemicals, potentially radioactive material and
cytotoxic drugs.
PRECAUTIONS TO BE TAKEN:
 Refer to Tab. 1 to check the products and handlings allowed and not.
Tab. 1 – List of handling and products

DESCRIPTION RISK
 Handling of non-pathogenic biological sterile materials ABSENT
 Preparation of non-toxic pharmaceutical substances ABSENT
 Plant cell cultures ABSENT
 Treatment of thin films ABSENT
 Transplantation on small animals ABSENT
 Assembly of electronic components ABSENT
 Handling of pathogens (see in INTENDED USE) BIOLOGICAL
 Handling of products potentially contaminated by

BIOLOGICAL
pathogens (see in INTENDED USE)
 Handling of chemicals CHEMICAL
 Preparation of cytotoxic drugs CHEMICAL/TOXIC
 Generic use of radionuclide RADIOACTIVE
allowed use not allowed use
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS FOUND AND
FOR DAMAGES CAUSED TO PERSONS AND/OR THINGS DUE TO MISUSE, UNAUTHORIZED USE,
TAMPERING OR ANY GENERAL USE OF THE EQUIPMENT THAT IS NOT PERMITTED OR THAT DOES
NOT COMPLY WITH THE OPERATING INSTRUCTIONS CONTAINED IN THIS MANUAL.
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DECLARATION OF CONFORMITY
The Manufacturer:
BioAir S.p.A.
Via Lombardia, 12
27010 Siziano (PV) - Italy
HEREWITH DECLARES THAT THE FOLLOWING PRODUCTS:
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MODEL: SafeMate Eco ABC
SIZES: 0.9 - 1.2 – 1.5 - 1.8
CODES: LDM3802 - LDM4802 - LDM5802 - LDM6802
DESCRIPTION: CLASS II BIOLOGICAL SAFETY CABINETS
COMPLY WITH THE FOLLOWING STANDARDS:
 IEC 61010-1:2010 Safety requirements for electrical equipment for measurement,

+A1:2016 control and laboratory use. Part 1: General requirements.
 IEC 61326-1:2012 Electrical equipment for measurement, control and laboratory use
- EMC requirements - Part 1: General requirements.
 AS 2252.2—2009 Controlled environments. Part 2: Biological safety cabinets Class II

—Design
OTHER ADDITIONAL HARMONISED EUROPEAN STANDARDS PARTIALLY APPLIED:
 EN 12469:2000 Biotechnology - Performance criteria for microbiological safety

cabinets.
APPROVALS AND CERTIFICATIONS

Any approval marks are listed in the DATA SHEET table (see 3.3).
NOTE: obtained approval marks refer solely to the standard cabinet, floor stand, germicidal UV-C
light and installation in "recirculation of exhaust air in the environment" mode.
Changes or different configurations invalidate the approval markings obtained.
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CONTACTS
If you require any further information or explanation regarding the contents of this manual, and for any
request relevant to
 service
 spare parts
 technical enquiries
contact:
WITHIN ITALY ABROAD

SAT Italy
+ 39 0382 6672.1 contact your local distributor or
sat@bioair.it sat@bioair.it
For any additional information, please contact the manufacturer:

BioAir S.p.A.
Via Lombardia, 12
27010 Siziano (PV)
+ 39 0382 6672.1
info@bioair.it
Check the manufacturer’s label (see MARKING) to communicate to the concerned staff for the cabinet
code and serial number.
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PART 1:
USER
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Part 1: User
ORIGINAL INSTRUCTION 1
1. ASSESSMENT OF RISKS
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SafeMate Eco ABC is a Class II Biological Safety Cabinet according to AS 2252.2—2009.
NOTE 1: notwithstanding conformity with the above standard, during operating of the cabinet some
residual risks remain and were assessed in order to indicate the measures to be taken to prevent the
hazards arising from normal use and foreseeable misuse.
The risks considered are the following:
 General
 Misuse
 Electric shock
 Biological
 UV-C radiations
 Pinch
 Spread of fire from the equipment
 Liberated gases and explosion
The analysis was done as per IEC 61010-1:2010/A1, 7.3.3 and Annex J.
The residual risks have been assessed as per the following Tab. 2:
Tab. 2 – Risk Category
CATEGORY ASSESSMENT DESCRIPTION
1 Broadly This Category fulfils the requirement for tolerable risk.
acceptable.
2 As low as This Category does not automatically fulfil the requirement for
reasonably tolerable risk. If possible, reduce these risk to Category 1.
practicable.
If not possible, then the instructions should contain a description of
the risk so that the Responsible Body can take appropriate steps to
protect the safety of operators.
3 Intolerable. This category contains risks that are not tolerable risks.
NOTE 2: no residual risk of this equipment belongs to Category 3.
NOTE 3: read the following warnings before operating this isolator.

Additional warnings shall be shown at the specific paragraphs.
NOTE 4: other risks associated with transportation, installation, electrical connection, installation of
accessories, as well as maintenance and/or service in general, are also described in Part 2 in the
relative paragraphs.
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS FOUND
AND FOR DAMAGES CAUSED TO PERSONS AND / OR THINGS DUE TO MISUSE,
UNAUTHORISED USE, TAMPERING OR ANY GENERAL USE OF THE EQUIPMENT THAT IS NOT
PERMITTED OR THAT DOES NOT COMPLY WITH THE OPERATING INSTRUCTIONS CONTAINED
IN THIS MANUAL.
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Part 1: User
1.1 General hazard. Risk category: 1

 The cabinet is supplied “NOT READY TO USE”.
 The use of this BSC before installation and testing has been carried out
can compromise the safety of the equipment and cause serious risks for
WARNING! the operator.
Taken measures to reduce the risk:
 Warning marking (caution) is provided on the cabinet.
 Adequate information for user.
 DO NOT operate this cabinet before reading this manual entirely.
 All operations required for installation and commissioning (unpacking,
positioning, assembly, installation of optional accessories, connection to the
mains power supply and testing) must only be carried out by specialized and
authorised personnel, in accordance with the instructions listed in PART 2 of this
manual (Installation and Maintenance).
 DO NOT connect the cabinet to the mains before it has been correctly installed,
put into operation and provided with a final test report that confirms the correct
performance as per the factory document.
1.2 Misuse hazard. Risk category: 1
 Misuse (see paragraph DEFINITION OF IMPROPER USE) compromises the

safety of this BSC and can become a biological hazard for personnel,
environment and product.
WARNING!
 The KEY of the start/stop switch is removable in both ON and OFF position.
 Read carefully this manual before using this BSC.
 Keep this manual always and easily available.
 Allow this BSC to be used only by authorized and properly trained personnel.
 Prevent unauthorised people or general public access to this cabinet.
 Never leave the switch KEY inserted.
 Keep the switch KEY in a safe place to avoid the possibility of using this BSC by
unauthorised personnel.
1.3 Biological hazard. Risk category: 1

 Operating modes other than MODE 1 (see 5.2.1) do not provide
protection against biological risk.
WARNING!  In an alarm condition the protection is compromised or absent.

 In the event of a blackout, protection is absent, but it is not signalled
by an alarm.
 In MODE 1 an unsafe condition is alerted by an acoustic and visual alarm.
 Modes other than MODE 1 are indicated with a visual alarm.
 Set up a blackout alerting system.
 DO NOT operate when alarm is on.
 Follow the instructions indicated in the event of an alarm in MODE 1 (5.2.1.6).
 Call servicing organization.
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Part 1: User

 This BSC protects neither the operator nor products from
contamination deriving from massive spilling of substances or use of
inadequate techniques.
WARNING!
 The use of this cabinet does not reduce the importance of compliance
with rules, procedures and behaviours used to ensure safety.
 Follow the procedures and guidelines, general and laboratory, relating to the
behaviours to be kept and the clothes and PPE to wear during the processing of
the substances used and in emergencies.
 Prepare an adequate emergency cleaning procedure previously validated.
 Wash hands and arms with a germicidal soap before and after work.

 The obstruction of intake grilles in the work chamber compromises the
performance of this BSC, with risk for operator safety and product
protection.
WARNING! The front grille of the working surface for the air inlet ensures the containment
of contaminated air inside the cabinet and protection of the product from
contamination of the external environment.
The intake grille on the rear wall balances the suction of the front one, allowing
an optimal division of the vertical laminar flow. This ensures a correct flow
distribution and an adequate containment by the front barrier.
 Keep always free from any object the grilles.
 Pay attention to paper, plastic film and cloths for cleaning.

 The use of the cabinet carries the risk of biological contamination
within it and exposes to this risk both the operators and the staff
present in the same area, both the assistance staff technique and
WARNING! maintenance, as well as no longer guarantee sterility environment in
which a product is manipulated.
Maintenance work such as replacing filters or other parts that can be
contaminated, need for moving or decommissioning, or emergency situations
due to errors or accidents, expose certainly at this risk.
Consequently, in these cases an appropriate decontamination of the cabinet is
mandatory.
 A validate method is described in PART 2 of this manual (see 9.1).
 Warning marking (biohazard) is provided on the cabinet.
 Authorize qualified and trained personnel to use this BSC.
 Cabinet users should identify the method of effective decontamination against
agents used.
 Prepare an SOP that allows effective decontamination in emergencies.
 Decontamination should exclusively be performed by personnel specialized and
authorised, following the instructions in PART 2 of this manual (paragraph 9).
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Part 1: User

UV rations hazard. Risk category: 1
 Breakage of the windows compromises both the containment due to
the barrier air, with risk for the safety of the operators and the
protection of the product, and the protection against UV-C radiation.
WARNING!
 The windows are made of glass and support an impact energy equal to 4 J.
The test was performed according to IEC 61010-1, paragraph 8.2.2, with
smooth steel sphere of mass 500g ± 25g in a vertical drop of 80 cm on a
horizontal surface.
 Minimum required impact energy value = 5 J
Lower values are acceptable up to 1 J, if more protective measures are taken.
 The windows are of safety (2 layers laminated type), to avoid that their
breaking suddenly causes the lack of the front air barrier and the protection
from UV-C rays.
 Warning marking (caution) is provided on cabinet glass windows.
 Prevent access to this BSC to unauthorised people or general public.
 Authorize the use of this cabinet only to trained and informed personnel.
In case of breakage of the windows:
 keep people away from the cabinet;
 do not run or switch immediately off UV-C function and protect
immediately eyes and skin;
 immediately suspend the work in progress;
 close if possible the front aperture;
 shut down the cabinet and prevent reusing; and
 call servicing organization.
1.8 UV-C radiations hazard. Risk category: 1

 This cabinet is equipped with UV-C light.
 UV-C radiations exposure causes damage to eyes (photoconjunctivitis
and photokeratitis) and skin (erythema and skin cancer).
WARNING!  UV-C radiations generate ozone which may be an inhalation hazard.
 UV radiation causes degradation of certain materials that may be used
in cabinet construction. The breakdown of some materials within the
work zone may result in the emission of toxic vapours.
 Glasses used contain at least 98% of the UV-C radiation emitted.
 The closure of the front access by lowering the glass window allows
containment of UV-C radiation inside the cabinet.
 A safety system consisting of two microswitches prevents the UV-C lighting
from turning on until the front access is fully closed.
 Access by key switch restricts the cabinet use, preventing unauthorised use.
 The supplied UV-C lamp complies with IEC/EN 62471.
 Warning marking (caution) is provided on cabinet glass windows.
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 Read the instructions regarding the use of germicidal UV light (see 5.1.4).
 Prevent access to the place where the cabinet is installed to unauthorised
people or general public.
 Authorize the use of this BSC only to trained and informed personnel.
 Keep the key of the switch in a safe place to avoid the possibility of using the
cabinet by unauthorised personnel.
 Use spare UV-C lamp recommended by BioAir only.
 DO NOT plug any additional UV-C fixture to the service socket.
 Do not stand close to the cabinet with the UV-C light on and visible, either
direct or reflected (see 5.1.4.1).
1.9 Pinch hazard. Risk category: 1
 The front window is electrically operated: its downward movement may

cause crushing at body parts or damage to objects.
WARNING!
 The motion is visible and the velocity is low.
 The motion is possible only by continued action of the user.
 During the motion an audible and visible alarm is activated.
 Warning marking (caution and pinch hazard) is provided on protective glass
windows.
 DON’T leave the hands and other parts of body or objects across the front
aperture when operating the sliding mechanism.

 During the front window lowering, a mechanical blockage may occur
with continuous unwinding of the belts as a result of which the window
would no longer be supported by the belts. A sudden unlock would fall
WARNING! the window resulting in a risk of serious damage to parts of the body or
to things that were on the path of fall.
 Automatic window lowering functions are removed, except for ECO MODE (see
5.2.1.5).
 The mechanical blockage of the window lowering is detected via firmware, with
consequent deactivation of the winding motor.
Starting from the working position, deactivation occurs within 2 s if the position
sensors do not change state.
Starting from an undefined position, deactivation occurs within 4 s if a position
with sensors (working position or all-closed position) is not reached.
 An additional microswitch is fitted to detect the loosening of the support belts
due to the unwinding.
If this occurs, the return to rest of the microswitch cuts off the power supply to
the winder.
 The positions in which the window movement stops automatically are only
working, ECO MODE and all-closing positions.
 In case of window jamming during the downward movement, DO NOT insist on
the action and proceed as indicated in 5.1.3.1.
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Electric shock hazard. Risk category: 1
 The cleaning of the front window involves lifting the control panel and
releasing mechanical parts, which would also allow access to moving
parts and hazardous live parts, with risk of crushing parts of the body
and risk of electric shock.
WARNING!

 A warning marking (disconnecting the power supply) is affixed to the filter
compartment access panel.
 The control panel can only be opened by authorized personnel in possession of
the key to open the control panel.
 Disconnect the cabinet from the power supply before lifting the control panel.
 This operation must only be carried out by adequately trained and authorised
personnel only, following the cleaning instructions for the front window given in
this manual (see 7.1.3).
1.12 Spread of fire from the equipment hazard. Risk category: 1

 On the cabinet it is possible the use of flame (optional tap for flammable gas)
during normal use.
 Sudden lack of vertical air flow (e.g. failure of the motorblower) causes
WARNING! the flame to rise up to close to the filter, causing a serious fire hazard.
 The flammable gas line is equipped with a normally closed 2-way solenoid valve
which is disabled in any case of alarm conditions, to block the flow of gas and
turn off the flame.
 Warning marking (caution) is provided close to the gas tap.
 Connect the devices for the use of flames only to this dedicated gas line.
1.13 Liberated gases and explosion hazard. Risk category: 1

 In case of use of flammable gas, consider that the cabinet has no gas
detection device.
 The interruption of gas supply is not detected: if it occurs during a use
WARNING! of flames, when it comes back, both the tap and the solenoid valve
would be open with consequent leakage of gas.
(The solenoid valve does not intervene in such cases, see 1.12).
 See also 2 in 2.4.

 Check that the flammable gas is supplied by the dedicated line of the cabinet.
 Use Bunsen burner only with flame control or piezoelectric ignition.
 DO NOT leave the cabinet with open flames.
 Refer to all guidelines about the use of flammable gas.
 Activate the solenoid valve only if necessary.
 Keep the gas tap closed if flames are not used.
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 The use of inert (non-reactive) gases can give rise to under-
oxygenation phenomena which can lead to an attenuation of attention,
a deformation of judgment and, in a short time, to brain injury.
WARNING!  Symptoms such as drowsiness, fatigue, errors of evaluation can be
masked by a state of euphoria which induces a false sense of security
and well-being.
 Loss of consciousness and death occur without any warning or alarm
feeling.
 The O2 content present in an environment can only be detected by
suitable instruments.
 Perform a risk analysis relating to the dangers deriving from the use of the inert
gas supplied.
 Periodic check of the tightness of the circuits.
 Bring vents to the outside.
 Provide adequate ventilation of closed environments.
 Report the possible presence of under oxygenated atmospheres.
 Consider installing specific sensors.
 Keep in mind that gas masks are not effective.
 Keep the gas tap closed if not used.
 Consult safety officer for precautions requested by local authorities.

 Decontamination techniques can cause risks for operators.
 The proposed decontamination method uses hydrogen peroxide (H2O2).
WARNING! NOTE:
 hydrogen peroxide is toxic by inhalation, in contact with skin and if
swallowed;
 causes serious eye damage;
 the threshold limit value (TLV) in the air varies according to the
legislation of your Country.
 Check the TLV or TWA allowed by the legislation of your Country.
 Decontamination shall be carried out exclusively by specialized and authorised
staff, following the instructions listed in par. 9 (PART 2 of this manual).
 Read the safety data sheet of the decontaminant used in advance.
 Personal protective equipment (PPE) should be available on the spot to protect
the personnel against the risks indicated.
 DO NOT stay in the room during decontamination activity.
 Provide adequate equipment for reading the concentration of H2O2.
 Consult safety officer for precautions requested by local authorities.
 If another decontamination method is chosen, perform a risk analysis related to
the dangers deriving from this other method.
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2. INSTALLATION
WARNING!
This cabinet is not suitable for operating in:
 residential areas.
 potentially explosive atmospheres.
On receiving the equipment make sure that the supply corresponds to what has been ordered.
In case of non-compliance report immediately to BioAir (see CONTACTS).
Also check it has not been damaged during transport.
WARNING!
 This cabinet is supplied “NOT READY TO USE”.
 See ASSESSMENT OF RISKS, warning 1.1
 The operations required for installation and commissioning (unpacking, positioning,
assembly, connection to the mains power supply, installation of optional accessories and
testing) must only be carried out by specialized and authorized personnel, in accordance
with the instructions listed in PART 2 of this manual.
 Ask your dealer or BioAir for commissioning.
THE SAFETY OF ANY SYSTEM INCORPORATING AND/OR INTEGRATES THIS CABINET IS THE
RESPONSIBILITY OF THE ASSMBLER OF THE SYSTEM.
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS THAT ARE
MANIFESTED AND FOR DAMAGE CAUSED TO PERSONS AND/OR THINGS DUE TO NON-
AUTHORISED INSTALLATIONS AND/OR WHICH ARE NOT COMPLIANT TO THE OPERATING
INSTRUCTIONS CONTAINED IN THIS MANUAL.
2.1 CHOICE OF LOCATION

WARNING! Biological and chemical hazard.
Air conditioning grilles/aisles (Pic. 2), air-draft and foot traffic (Pic. 3) may have a negative
influence to the cabinet ventilation.
An unsuitable location may compromise cabinet performance and operator safety.
 Install this BSC in an air-draft free room, without air conditioning grilles and aisles and
avoid corridors where foot traffic is intense (eg. Pic. 4) to not interfere with the air barrier
crossing the cabinet front aperture.
 The distances to be maintained around the cabinet and above it are defined in 3.6.
 Other features of the location are available for the installer in Part 2 of this manual
(paragraph 8.2).
Pic. 2 Pic. 3 Pic. 4
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2.2 FLOOR STAND

WARNING! Instability hazard.
Inadequate positioning can endanger the stability of the cabinet and pose a serious danger to
the operator and personnel involved around it.
 Fix the cabinet on the stand specially designed by BioAir.
NOTE: the use of other ways to support the cabinet is discouraged and is under full the
responsibility of the customer.
WARNING!
This operation should be carried out by service specialized and authorised personnel.
Installation must be carried out in accordance with the instructions in the Part 2 of this
manual (see 8.3.1.1 and 8.3.1.2) and in accordance with the instructions supplied with the
accessory.
THE SAFETY OF THIS CABINET DEPENDS ON THE CORRECT INSTALLATION OF THE ACCESSORIES
ACCORDING TO WHAT IS INDICATED IN THIS MANUAL AND IT IS THE RESPONSIBILITY OF THE ASSMBLER.
The support is available in the list of optional accessories in fixed or adjustable height version.
Height and other features of the stands are indicated in 3.10.1.
2.2.1 Restrictions on adjustable stand use

The adjustable floor stand must be assembled for the desired height.
The height is adjustable only at predetermined steps, authorised according to the cabinet models
based on the stability tests carried out. The steps authorised are shown in Tab. 3.
NOTE 1: the result of the stability test allows the use of the support in combination with the
+
SafeMate Eco as per height limits shown in Tab. 3.
The stability tests were made in accordance with IEC 61010-1 paragraph 7.4.
NOTE 2: the choice should be defined at installation phase. Any variations must be carried out
by qualified and authorised technical personnel.
Tab. 3 – Authorised heights

+ floor stand / work surface (mm)
SafeMate Eco ABC
SIZES 690 / 790 730 / 830 770 / 870 810 / 910 850 / 950
0.9
1.2
1.5
1.8
allowed not allowed NA: NOT AVAILABLE
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2.3 CONNECTING TO THE MAINS
WARNING!
 See ASSESSMENT OF RISKS, warning 1.1
 The user should not connect the cabinet to the mains before commissioning and final
testing.
 The first connection should be carried out only by authorised personnel in accordance with
the instructions in the Part 2 of this manual (see 8.5).
The cabinet is designed to operate in the range defined by the technical data sheet (see 3.1)
and in the environmental conditions indicated in 3.4.
 Check that the mains voltage matches what shown on the data plate of the cabinet.
 Use a dedicated power line equipped with a ground connection that complies with local
regulations.
 The magneto-thermal protection to be used on the power line is indicated in Part 2 of this
manual (see 8.5).
 If using an uninterruptible power supply (UPS), read the information in Part 2 of this
manual (see 8.5).
Then proceed as follows:
1. Plug the female connector (Pic. 5 – item 1) of the supply cord (2.3.1) to the appliance inlet (Pic. 7 -
item 1).
2. Insert the clamping hook (Pic. 6) into the threaded insert (Pic. 7 - item2) by screwing it firmly to
the top of the connector in a slightly forced (hand) manner.
NOTE 1: the hook must be removable without the use of a tool.
Pic. 5 Pic. 6 Pic. 7
3. Connect the plug (Pic. 5 – item 2) of the supply cord (2.3.1) directly to the
dedicated mains socket (2.3.2).
NOTE 2: do not use extension.
When the connection is complete, the power input point

appears as shown in Pic. 8:
1. Appliance inlet (see also 2.3.3).

2. Supply cord connector (see also 2.3.1).
3. Appliance coupler clamping hook.
Pic. 8
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2.3.1 Mains supply cord

The mains supply cord is a detachable flexible cord with female mains connector C13 as per IEC
60320 (see Pic. 9 – item 1) and a type I plug (10 A rated) as per AS/NZS 3112 (see Pic. 9 – item 2)
moulded at either end.
The cable length is 3 m.
It complies with IEC 60227 or IEC 60245 and it is approved by a Competent Body.
The technical features are available in Tab. 8.
AUSTRALIA
Pic. 9
BioAir may supply on request the following optional detachable supply cords:
UNITED KINGDOM SWITZERLAND GERMANY / FRANCE
WARNING!
An unsuitable or defective mains supply cord compromises the safety of the cabinet.
 Do not cut the cord to replace the plug or connector.
In case of incompatibility with mains socket or damage of the
cord, plug or connector, ask the maintenance staff to replace the
complete cable with a certified type and with the characteristics
indicated in Tab. 8.
2.3.2 Mains socket
WARNING!
The means for disconnection of the BSC from the mains is the plug of the supply cord.
The mains socket to be used should be choosen by specialized and authorised technical
personnel, in compliance with the instructions also given in Part 2 of this manual (see
8.5.1).
 The mains socket should be dedicated and located as close as possible to the cabinet
(max 2 m) according to the length of the supply cord (do not use extension), but out
of the reach of the user’s arms (to avoid the cabinet be switched off accidentally).
 Mark the socket as exclusive use for this BSC.
 Neither objects nor furniture should be hidden the connection in order to can quickly
disconnect the cabinet in case of emergency.
The technical features are available in Tab. 7.
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2.3.3 Appliance mains inlet and main fuses
The appliance inlet (Pic. 10) is placed on the right top of the
control panel (see Pic. 15 – item 1) and consists to a male
connector (IEC 60320 C14) with a fuse holder for two main
fuses (Pic. 11).
The main fuses are replaceable from the outside, located in
the inlet appliance.
A label close to the appliance inlet reports the values of the Pic. 10 Pic. 11
fuses (see Pic. 12).
Fuses rating are also available in the table of specifications
(see 3.1):
Pic. 12
2.4 CONNECTION OF GAS LINES (OPTIONAL)
WARNING! See ASSESSMENT OF RISKS, warnings 1.12 1.13 and 1.14
On the cabinet there are two lines for technical gases and vacuum.
To take advantage of these utilities, specific (optional) taps must be installed, supplied separately in the
installation KIT (see 5.1.5).
On the flammable gas line must be installed the solenoid valve provided in the dedicated installation KIT.
WARNING!
The operation should be carried out exclusively by service specialized and authorised personnel.
Installation must be carried out in accordance with the ruling standards and the instructions
given in both Part 2 of this manual (see 8) and in those su supplied with the accessory.
The installer must issue a test report for the activation of these lines.
THE SAFETY OF THIS CABINET DEPENDS ON THE CORRECT INSTALLATION OF THE ACCESSORIES
ACCORDING TO WHAT IS INDICATED IN THIS MANUAL AND IT IS THE RESPONSIBILITY OF THE ASSMBLER.
2.5 EXHAUST AIR TREATMENT

This BSC allows the recycling of its own exhaust air into the laboratory.
In case of request to duct the exhaust air outside, accessories designed by BioAir may be supplied to
facilitate connection to the ducting.
For this type of need, contact BioAir, which, directly or through local distributors, will provide the
information necessary to identify the accessories suitable for the specific needs of the laboratories, in order
to easy the solutions to be adopted.
WARNING!
 Do not connected directly to any other air-handling system.
NOTE: it may be provided exhaust the air to the atmosphere through an indirect connection,
and possibly to further purify the exhaust air, e.g. through an absorbent filter.
 Such changes must be carefully evaluated and designed by competent personnel in order not
to cause unbalancing of the airflows with consequent risk for the safety of the operator, the
environment and the product.
 Any removal of this accessory must also be analyzed by competent personnel, as the risk of
compromising safety is the same.
 These operations should be carried out only by service specialized and authorised personnel,
in accordance with the project, with the instructions in the Part 2 of this manual (see 8.3.3)
and in accordance with the instructions supplied with the accessory.
 Contact companies specializing in these ducting.
 Both in the case of installation and removal of this modification, the cabinet should be
validated by qualified personnel.
CHANGES LIKE THIS OR DIFFERENT CABINET CONFIGURATIONS WILL VOID ANY APPROVAL
MARKING IF APPLICABLE.
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2.6 FINAL PROCEDURES
WARNING! See ASSESSMENT OF RISKS, warning 1.1
WARNING!
 Before the first use of this BSC an efficiency test (installation testing) shall be carried out as
per AS 2252.2—2009, paragraph 5.2 (Critical performance tests for cabinet function) and Part
2 of this manual, paragraph 8.7, by a qualified and authorised staff, with the issue of an
installation report to be kept along with the documentation of the cabinet.
This makes it possible to exclude any damage caused by transport or the installation itself,
which could affect the good operating and the safety of the cabinet.
The test should be performed by qualified and authorised staff, with the issue of relevant
report to be kept along with the documentation of the cabinet.
WARNING!
 Do not operate this BSC without installation report.
Once installation is over, carry out the final procedures before starting work:
 turn on the cabinet in MODE 2 (see 5.2.2) and open the front access area lifting the
window by the relevant key (see 5.1.7.8);
 turn off the cabinet and disconnect it from the mains (see 5.2.3.2).
 remove the work surface (see Pic. 13,

item 2) using the tool indicated in Pic.
29); then remove the front grille (see
Pic. 13, item 1);
 carefully clean the cabinet inside and
outside following instructions reported
at relevant paragraph (see 7.1);
 refit the frontal grille (Pic. 13, item 1)
and the work surface (Pic. 13, item 2)
in its position (see 5.1.6) after
checking that no foreign body is under
it;
NOTE: the plastic pipe under the

work surface on the left side (see Pic.
13, item 3) is for the exclusive use of Pic. 13
technical service personnel.
 Attach the SHORT INSTRUCTIONS label , supplied with the cabinet

documentation, to the control panel (see Pic. 13, item 4).
 Attach the BIOLOGICAL HAZARD WARNING label (see MEANING OF SYMBOLS),

supplied with the cabinet documentation, on the outside part of the door of the room where
the cabinet operates.
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3. TECHNICAL DATA
DESCRIPTION SIZE 0.9 SIZE 1.2 SIZE 1.5 SIZE 1.8
3.1 POWER SUPPLY

Mains supply voltage (V~): 220-240
Mains supply frequency (Hz): 50/60
Required power line (W):
1050 1175 1250 1475
(720 W service socket included)
Current (A): 4,9 5,3 5,6 6,3
Absorbed power (W):
200 325 400 625
(fan and light on only)
Main fuses rating: steatite, 5x20, F10A H, 250 V, I2t: 121
3.2 REFERENCE STANDARDS
SAFETY: IEC 61010-1:2010/A1:2016
Electrical insulating protection class [IEC 61140]: I
ELECTROMAGNETIC COMPATIBILITY (EMC): IEC 61326-1:2012
MICROBIOLOGICAL SAFETY: AS 2252.2-2009; EN 12469:2000
Microbiological class protection [AS 2252.2; EN 12469]: II
IP protection degree [IEC 60529]: Ordinary equipment (IP xxB)
3.3 DECLARATIONS AND APPROVALS
Mark of conformity:
-
Approvals:
-
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3.4 USE ENVIRONMENTAL CONDITIONS

Electromagnetic operating area: industrial
Use: indoor
Altitude (m): up to 2000
Temperature (°C): from 10 to 35
Maximum relative humidity (%): 80 for temperatures up to 31 °C, decreasing linearly to 55 at 35 °C
Max MAINS supply voltage fluctuations (%): up to ±10
TRANSIENT OVERVOLTAGE CATEGORY: II
POLLUTION DEGREE: 2
3.5 TRANSPORT AND STORAGE CONDITIONS
Ambient temperature (°C): from -5 to 45
Relative humidity (%): up to 90
Atmospheric pressure (mbar): from 800 to 1060
3.6 WEIGHT AND DIMENSIONS
Weight (kg):
210 245 275 335
(without floor stand)
Overall dimensions L x D x H (mm):
1075 x 795 x 1450 1380 x 795 x 1450 1685 x 795 x 1450 1990 x 795 x 1450
(without floor stand)
Free space needed around the cabinet (mm):
500/500/300/650
[left/right/top/front]
Height of the work surface from the cabinet bottom (mm): 100
Front aperture dimensions L x H (mm):
860 x 195 1165 x 195 1470 x 195 1775 x 195
(operating height - safe)
Front aperture maximum height H (mm):
400
(cleaning and loading height - unsafe)
Front aperture in ECO MODE H (mm): 50
Working space dimensions L x D x H (mm): 925 x 580 x 700 1230 x 580 x 700 1535 x 580 x 700 1840 x 580 x 700
Safe working area dimensions L x D (mm): 725 x 350 1030 x 350 1335 x 350 1640 x 350
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3.7 PERFORMANCES
Intended life of the equipment (years): 10
Laminar Air Flow mean velocity [AS 1807.1](m/s): 0.40 ÷ 0.45
Inflow Air Barrier mean velocity [EN 12469](m/s): 0,85 ±10%
3
Exhaust Air flow rate (m /h): 450 ±10% 550 ±10% 700 ±10% 800 ±10%
Exhaust Air flow ratio (%): 35 ±10%
Containment at the aperture

-Apf - Aperture Protection Factor [EN 12469; AS 1807.26]: ≥1.0 x 105
-Penetration of the test aerosol [AS 1807.22](%): < 0.01
Working space air cleanliness class [EN 14644-1]: ISO 5
Illuminance [EN 12469] (lux): >750
Sound level [EN ISO 3744] (dB[A]): <58 <60
Vibration [EN 12469] (mm RMS): <0.005
Max increase inside cabinet in temperature from the ambient [EN
<5
12469] (°C):
Leaktightness index of the cabinet housing [EN 12469]: LI-C
Cleanability index [EN 12469]: CI-B
Sterilizability index [EN 12469]: SI-B
3.8 MATERIALS
3.8.1 METAL PARTS
Main structure: cold rolled steel, stove enamel coated
Walls inner surface of the working area: stainless steel AISI 304 - SB finishing
Working surface: stainless steel AISI 304 - SB finishing
Maximum load possible on working surface (kg):
[uniformly distributed] 15
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3.8.2 GLASS PARTS

Type: 2 layers laminated safety glass
Front window thickness (mm): 3+3 4+4
Side windows thickness (mm): 3+3
UV-C radiations minimum retention (%): 98
Impact maximum energy sustainable (J):
4
[IEC 61010-1, clause 8.2.2]
3.8.3 FILTERS
LAF filters dimensions L x D x H (mm): 915 x 610 x 68 1219 x 610 x 68 1525 x 610 x 68 1830 x 610 x 68
Other features of LAF filter: fabric equalizer downstream
EXH filter dimensions L x D x H (mm): 457 x 457 x 115 610 x 457 x 115 762 x 457 x 115 915 x 457 x 115
Filters efficiency class [EN 1822-1]: H14
Filters global MPPS efficiency [EN 1822-1](%): 99.995
MPPS diameter [EN1822-1](µm): 0.1 ÷ 0.3
Expected average life (h): 6000 (1)
WARNING! Using filters other than the original ones can compromise cabinet performance and therefore operator safety.
Check spare part list (see 10.3).
(2)
3.8.4 LIGHTING
Type of lamp: fluorescent, tubular T8
Fluorescent lamps power (W): 2x 25 2x 30 2x 36 2x 58
Lamp light colour 840
Lamp colour temperature (K): 4000
Average life at 90% yield (h): 12000
(1)
see information in 10.1.2
(2)
see alternative in 3.10.4
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3.8.5 UV-C LAMP

Type of lamp: UV-C, tubular T8 tubular T10
UV-C lamp power (W): 1x 15 1x 30 1x 40
UV-C spectral peak (nm): 253.7
UV-C lamp average life (h): 8000
UV-C radiation (W): 4.8 11.6 14.9
3.9 OUTLET
3.9.1 SERVICE SOCKETS
Supply voltage (V): mains
Maximum load (A):
3
[distributed on all used sockets]
Minimum IP protection degree [IEC 60529]:
44
[with cover closed]
3.9.2 CONNECTOR VFC
Output type: contact NO – voltage free
Maximum applicable voltage (V): 24 SELV-PELV
Maximum load (A): 1
3.10 OPTIONAL ACCESSORIES FEATURES

3.10.1 FLOOR STAND
Material: steel tube, stove enamel coated
Non-adjustable floor stand height (mm): 730
Adjustable floor stand height [min – max] (mm): 690 – 810 (1)
Non-adjustable floor stand weight (kg): 18 19.5 20.5 22

Adjustable floor stand weight (kg): 25 26 27 29
(1)
see 2.2.1
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3.10.2 INERT FLUIDS UTILITY

Valve type: fine regulation head-valve with PTFE seal tap
Use: Inert gases, vacuum, compressed air
Knob material: polypropylene
Max operating pressure (kPa): 300
Inlet port thread [internal / external]: G1/4” / G3/8”
3.10.3 FLAMMABLE GAS UTILITY
Valve type: ceramic head-valve tap with safety lock
Usable categories of gases [EN 437]: 1–2-3
Knob material: polypropylene
Max operating pressure (kPa): 2
Inlet port thread [internal / external]: G1/4” / G3/8”
Solenoid valve inlet thread [female]: G1/4”
3.10.4 LED LIGHTING
Type of LED: SMD 3014
Lamp power (W): 33 44 54 65
Minimum CRI: 90
Lamp colour temperature (K): 4000
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DESCRIPTION NOTES 0.9 1.2 1.5 1.8
3.11 ORDINARY OPTIONALS

AC10000 CHEST DRAWER 2 drawers – with castors
AS1L300 SUPPORT STAND SIZE 0.9   
AS1L310 SUPPORT STAND SIZE 0.9 ADJUSTABLE   
NOMINAL height = 730 mm
AZ1E601 ARMRESTS SM
AZ6H000 KIT SOCKET DE STD
AZ6H001 KIT SOCKET FR STD
AZ6H002 KIT SOCKET UK STD
AZ6H003 KIT SOCKET CH STD
AZ6H004 KIT SOCKET AU STD
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Part 1: User
4. OPERATING PRINCIPLE
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SafeMate Eco ABC is a cabinet of the type “VERTICAL LAMINAR AIRFLOW WITH PARTIAL
RECIRCULATION”, which provides protection for the operator, for the environment and for the product.
The effectiveness of this cabinet depends on several factors:
 location and installation test;
 training of personnel involved in the use;
 proper use and correct working procedures;
 correct handling of biological agents according to current regulation;
 routine and periodic maintenance; and
 routine performance testing (see 10.1.1).
The working area (Pic. 14 – item 1) is crossed
by a vertical unidirectional air flow
(DOWNFLOW, Pic. 14 – item 2).
This airflow is part of what supplied by a
motorblower (Pic. 14 – item 3), which blows into
a plenum (Pic. 14 – item 4) distributing the air
as follows:
 about 70% (Pic. 14 – item 11) conveyed
into the working chamber through an
absolute filter HEPA H141 (Pic. 14 – item
5) to create a sterile area in laminar flow
(LAF) in which the product is protected
from external and cross contamination;
NOTE: near the work surface the

airflow divides (Pic. 14 – item 9) between
the front and rear intake grilles.
(In case of a perforated work surface it
crosses it with a small part of the airflow
sucked by the rear grille.)
 about 30% conveyed to the outside of the
cabinet (Pic. 14 - item 7) through an
absolute filter HEPA H141 (Pic. 14 – items
6) to draw an equal inward airflow volume Pic. 14
through the front aperture (INFLOW, Pic.
14 - item 8) in order to form a protective barrier for the operator.
Both airflows are mixed by the suction of the fan, forming 100% of the (contaminated) airflow in the rear
intake duct (Pic. 14 - item 10) to repeat the cycle (Pic. 14 - items 11 and 12).
All potentially contaminated areas within the BSC that are at positive pressure are surrounded by zones of
negative pressure relative to the workroom atmosphere.
(1) HEPA H14 filtration assures both the air sterility of the working space and the air sterility of the
exhausted air.
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Part 1: User
5. OPERATING INSTRUCTIONS
5.1 CABINET DESCRIPTION
LEGEND (see Pic. 15)
1. Mains inlet appliance with main fuses

2. Locking hook for mains connector
3. Technical inlets and outlets
4. Data plate
5. Start/stop switch
6. Soft touch keypad with display
7. Alarm pilot light
8. Control box panel
9. Side windows
10. Front window bottom edge
11. UV-C lamp
12. Connection for flammable gas tap (see
5.1.5)
13. Connection for inert fluids–vacuum tap
(see 5.1.5)
14. Inlet for flammable gases (see 2.4)
15. Inlet for inert fluid - vacuum
16. Service sockets
17. Outer exhaust filter face
18. Lamps housing
Pic. 15 19. Control panel closing screws
20. Front intake grille
21. Slots for rear suction
22. Working surface
23. Floor stand1
24. Footrest
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Part 1: User
5.1.1 Lighting
The working chamber is lighted up by two
fluorescent lamps through the front window.
Alternatively, LED lighting can be installed on
request.
Location: inside the control panel (Pic. 15 -
item 18), out of the working area (see Pic.
16).
The values relating to the illumination of the
work area are available in 3.8.4.
It is activated in both MODE 1 and MODE 2 by
the key (LIGHT) of the keypad (see

5.2.1.1 and 5.2.2.1).
Lamps characteristics are available in 3.8.4.
This function is interlocked with UV-C light. Pic. 16
5.1.2 Service sockets
On the front wall of the working chamber there are two service sockets (see Pic. 17).
Location: see Pic. 15 - item 16.
To activate and deactivate
them, press the relative key
on the control keypad (see
5.2.1.2).
Technical features and the
limit of use are available in
3.9.1.
The supply voltage supplied
is the same as the mains.
Maximum current allowed (3
A) can be used on a single
socket or distributed
between two sockets.
Pic. 17
Standard equipment: type I, 15 A [AS/NZS 3112] (see Pic. 18).
NOTE: the current is limited as indicated in 3.9.1.

The line is protected by a fuse, located in the control panel.
They are controlled simultaneously via the key (SOCKET) on the control
keypad, in both MODE 1 and MODE 2. Pic. 18
Other types of electrical socket are available on request, as shown below:
UK France Switzerland Germany
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Part 1: User
5.1.3 Front window
 WARNING! See ASSESSMENT OF RISKS, warning from 1.7 to 1.9
The front window is made of laminated safety glass (two layers) and is part of the cabinet
enclosure: in addition to being a protection for the operator against UV-C radiations, constitutes
a decisive factor for the containment efficiency of frontal barrier (INFLOW).
Location: see Pic. 15 - item 10.
The breakage of the window compromises both the UV-C radiations protection and the air
barrier containment.
The front window (Pic. 19 – item 4) is sliding and it’s electrically
operated by UP and DOWN push buttons (keys) located on

the control keypad (see Pic. 37).
Movement occurs only by keeping the key pressed (except ECO
MODE; see 5.2.1.5) and it is automatically stopped in 4 preset
positions (see Pic. 19):
 working position (item 1);
to resume movement, release and press the button again.
 all-open position (item 2);
 all-closed position (item 3);
to resume movement, press the required button again.
 ECO MODE position (item 5).
Pic. 19
During operating in MODE 1 (see 5.2.1), any position other than the working one (see Pic. 19 –
item 1) will active the alarm (see Pic. 44).
Technical features and the limit of use are available in 3.8.
NOTE: the height of the opening corresponding to the working position determines the
velocity of the intake air through which the containment barrier is guaranteed to protect the
operator, without the risk of investing the product worked inside.
For this reason the working position height can not be changed.
During MODE 1 operating (see 5.2.1), any position of the window other than the working one
will active the alarm.
5.1.3.1 FRONT WINDOW MOVEMENT JAMMING
If the downward movement should stop to positions other than those provided in 5.1.3
despite pressing the dedicated key, do not insist and proceed as follows:
1. push the key (UP) for at least 2 seconds, since the window rises again;
2. then push the key (DOWN) and try to lower the window;
NOTE 1: if the problem persist shut down the cabinet, remove the key from the switch
and call service.
NOTE 2: in case a message appears as shown in Pic. 48, proceed in the same way.
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Part 1: User
5.1.4 UV-C germicidal lamp
The cabinet is equipped with

a UV-C germicidal lamp
fitted on the back wall of the
working chamber.
Location: see Pic. 20.
Technical features and limits
of use of the lamp are
available in 3.8.5.
The light emitted has a
wavelength of 253.7 nm,
(see Pic. 21), which is
Pic. 20
dangerous for the eyes and
skin ( see ASSESSMENT

OF RISKS, warning from 1.7
to 1.8).
The use of this lamp is
interlocked with fluorescent
light and enabled in MODE 2
(see 5.2.2) only, provided
that the front access is fully
closed.
The procedure to light it is
explained in 5.2.2.5.
The glass used for front
window has a minimum
shielding capability of 98%
UV-C radiation, thus
managing to block
dangerous radiation and
provide operators with a
light with a wavelength Pic. 21
greater than 400 nm (see
Pic. 22) and therefore not
dangerous.
For replacement, follow the
instructions described in 7.4
and sub-paragraphs.
NOTE 1: UV radiation is
not recommended for use in
BSCs (see EN 12469, annex
A.2).
Pic. 22
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Part 1: User
WARNING!
In case of access to biologically contaminated area, this sterilization method is not
adequate.
 In the event of a substantial spillage of contaminated liquids, in cases of technical
assistance and maintenance involving the replacement of filters or fan motors, and
before routine controls it is necessary to have an effective and complete
decontamination of the cabinet, with a validated method.
See par. 9 for information and decontamination method proposed by BioAir.
Effectiveness of germicidal effect depends on different factors:

 kind of micro-organism subjected to UV-C radiations (different kinds of microbial
species they have different reaction to UV-C radiations);
 time of exposure, with proportional linear dependence;
 distance of radiation surge from micro-organism has to be cured, with inversely
proportional quadratic dependence;
 power of radiation surge, because the effectiveness of sterilisation depend from real
power irradiated on zone to be cured;
 ambient temperature (at 0 °C the emission intensity is reduced by about 30%).
Therefore it’s necessary to take as reference the table reporting the needed dose of radiation to
supply (radiant exposure) expressed in J/cm2 to determine the time of exposure for each kind of
micro-organism (knocks, spores, moulds or ferments) to be eliminated.
The sterilizing cycle should be performed after any work session.
The efficiency of this method depends on right exposure time to UV-C radiations of the zone to
decontaminate.
The exposure time is calculated as follows:
t = He x (L)2
Ee (L’)2
where
He = radiant exposure required (μJ/cm2)
Ee = irradiance (μW/cm2)
L = source-surface distance (m)
L’ = rated distance (m)
NOTE 2: the fact that UV lamp is producing light is not directly related to its actual
neutralising power on microorganisms. The actual efficiency may be tested with proper devices.
On request it is possible to perform a test of efficiency of radiation emitted by the UV-C lamp:
contact your BioAir distributor.
5.1.4.1 PROTECTION AGAINST UV-C RADIATIONS

The protection supplied by the cabinet windows to the user is enough to guarantee an high
level of safety during light start, programming and for occasional and short standing in the
visible or reflected area of the UV-C lamp due to work needs.
WARNING!
We do not recommend long stays or workstations that can be reached by direct or
reflected UVC light.
 If it is essential to remain in the same room during the operation of the UVC
lamp, it is the customer's responsibility to carry out a risk analysis to take the
appropriate measures.
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Part 1: User
5.1.5 Gases utilities (optional)
WARNING! See information in 2.4
The taps can be installed individually or in pairs, in the configurations shown in Pic. 23 (item 1,
vacuum/inert-gas tap and item 2, flammable gas tap) or in Pic. 24 (items 1 and 2, taps for
vacuum/inert-gas).
NOTE 1: the installation of 2 flammable gas taps (see Pic. 25) is not allowed.
Pic. 23 Pic. 24 Pic. 25
NOTE 2: the provision of gas supply is not recommended. Flames from gas burners interfere
with laminar airflow within the cabinet and may compromise containment.
Where practicable, disposable loops or use of electrical loop sterilizers are preferred to gas
burners.
NOTE 3: VACUUM EQUIPMENT

Vacuum pumps must discharge into a connection canopy to ensure that any material is captured
by a HEPA filter. The vacuumed material, the pipes, the pump and all the connected instruments
must be disposed of as biological waste according to local or state standards.
The taps should be marked with

appropriate labels (Pic. 26 and Pic.
27).
Pic. 26 Pic. 27
WARNING! See 2.4
 The installation should be carried out exclusively by service specialized (see

8.3.2).
 Flammable gas line needs installation of a normally closed solenoid valve.
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Part 1: User
5.1.5.1 LINE FOR INERT GASES, VACUUM AND COMPRESSED AIR
Non flammable pressurised fluids line is equipped with a fine regulation head-valve with PTFE
seal tap.
The tap knob is made of grey polypropylene with black central mark (see Pic. 23 - item 1).
Fluids used must be non aggressive towards materials of which the valve is made (body in
brass, gaskets in PTFE).
The tap is also suitable for vacuum (see also 5.1.5, 3) and compressed air.
Features and limits of use are available in 3.10.2.
5.1.5.2 FLAMMABLE GAS LINE
Flammable gas line (see also 5.1.5, 1 and 2) is controlled by a normally closed solenoid
valve and a ceramic head-valve tap (with safety lock) in line.
Activation of the solenoid valve is only possible in MODE 1 (see 5.2.1) by pressing the key
GAS on the keypad, provided that no alarm is running.

Once activated, the solenoid valve opens and it is possible to use the gas by acting on the tap.
Gas supply is automatically cut off by the solenoid valve in the following events:
 shutdown of the cabinet;
 sudden interruption of the power supply (BLACKOUT);
 lack of airflow;
 activation of any alarm.
NOTE 1: termination of alarm conditions and automatic or manual restart of the cabinet
after blackout will not reactivate the valve. Restore can only be performed manually with
ceased alarm.
This safety measure is in accordance with EN 12469 standard, annex A.9 and AS 2252.2,
clause 4.9 to prevent HEPA filter damage and possible risk of fire (see 1.12).
The tap knob is made of yellow polypropylene with yellow central mark (see Pic. 23 - item 2).
Features and limits of use are available in 3.10.3.
NOTE 2: the provision of gas supply is not recommended. Flames from gas burners
interfere with laminar airflow within the cabinet and may compromise containment.
NOTE 3: where practicable, disposable loops or use of electrical loop sterilizers are
preferred to gas burners.
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Part 1: User
5.1.6 Working chamber

The working chamber is delimited at the top by the HEPA H14 filter for downflow ((Pic. 14 - items
2 and 5), laterally by two fixed windows (Pic. 15 - item 9), and at the front by a sliding window
(Pic. 15 - item 10) which allows access to the user's hands.
The internal front wall (see Pic. 17), on which two electrical service sockets (see 5.1.2) and two
predispositions for gas lines (see 5.1.5) are installed, is made of stainless steel.
The work surface and front grille (see Pic. 28) are made of stainless steel.
5.1.6.1 WORK SURFACE
The work surface is

supplied in a single tray-
shaped piece (see Pic. 28,
items 1).
Features and limits of use

are available in 3.8.1.
To remove it use the

supplied tool (see Pic. 29).
Pic. 28 Pic. 29
WARNING! Risk for the operator and the product.

Wrong positioning may compromise the safety of the operator and/or product.
 Pay attention to the insertion with the front grille (Pic. 28, item 2).
 Use the cabinet exclusively with the work surface placed.
5.1.6.2 SAFE WORKING AREA

This area is the part
of the working
surfaces, where the
correct value and
good distribution of
the laminar airflow
guarantees the
protection of the
product against both
the cross
contamination and
the airborne
contamination from
outside entering the
BSC through the Pic. 30
front aperture.
WARNING!
Operating outside the SAFE WORK AREA compromises operator safety and
product protection.
 Operate only within the SAFE WORKING AREA shown as top view in Pic. 30.
NOTE: also follow the indications listed in 6.3.

Details on the dimensions of the working area are available in relevant table (see 3.6).
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Part 1: User
5.1.6.3 INTAKE GRILLES
These grilles consist of slots suitably sized for

operator safety and product protection and should
always be kept free.
In particular they are necessary for:
 guarantee the front barrier (front
opening grille, see Pic. 31 - item 1);
 centre the vertical airflow as much as
possible (grille on the back wall of the
working chamber, see Pic. 31 - item
2);
Pic. 31
WARNING! Biological risk.

The obstruction, even if partial, of these intake air grilles may compromise
operator’s safety and the product protection.
 Check constantly that the grilles are always free.
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Part 1: User
5.1.7 CONTROL PANEL
5.1.7.1 Quarter-turn locks

Two locks (Pic. 32) are located to the right and left of the
control panel and allow it to be opened by dedicate key (Pic.
33) for front window complete cleaning (see 7.1.3.1) and for
lamp replacement (see 7.4.2).
Location: see Pic. 15 – item 19
Pic. 32 Pic. 33

Opening the control panel allows access to parts of the cabinet that are live.
 The release key must be kept by the laboratory manager and used exclusively
for the operations indicated above.
 Before lifting the control panel, disconnect the cabinet from the power supply.
5.1.7.2 Start/stop switch

 This switch is not the means of disconnecting the cabinet. The disconnection
must be done by disconnecting the mains power cord.
The key switch (Pic. 34) allows to switch ON/OFF the cabinet.
The key of the switch is removable in both ON (1) and OFF (0) position.
Reference: standard EN 12469, annex H.3.4.
Pic. 34
5.1.7.3 Alarm pilot light
NOTE: the alarm condition is signalled, as well as acoustically, by a red pilot light on the
membrane keypad and indicated with . The signal can be fixed or flashing. In MODE 2 it is
always activated to signal the unsafe mode.
In MODE 1 the alarm is also audible.
A second red LED indicator (Pic. 35) with redundant function is present at
the bottom right of the control panel, with increased lateral visibility as it
slightly protrudes from the casing.
It is activated simultaneously with the membrane keypad pilot light.
Location: see Pic. 15 – item 7. Pic. 35
5.1.7.4 Service outputs and inputs

On the top right side of
the control panel (Pic. 36),
to the left of the mains
power inlet (Pic. 36 -
items 1 and 2), there are
accesses (Pic. 36 - items
3, 4 and 5) available to
technical service staff for
Pic. 36
maintenance and optional applications (see 8.3.4, 8.3.5 and 8.3.6).
Location: see Pic. 15 - items 3.
Technical features and limits of use are available in 3.9.2.

The connector of Pic. 36 – item 5 is optional.
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Part 1: User
5.1.7.5 Key pad

The running functions of the cabinet
are activated by the push buttons
(keys) of the keypad (see Pic. 37)..
They are visualised on the
alphanumeric display and indicated by
pilot LEDs.
In user mode the keys of the keypad
have double functions:
 normal function (see 5.1.7.5)

access to operating MODE 1 (see
5.2.1) and MODE 2 (see 5.2.2).
 advanced function (see 5.1.7.9)
access to available setting and
information for user. Pic. 37
5.1.7.6 Display
The display is located in the centre of the keypad (see Pic. 37) and it contains all the
information about the cabinet status.
5.1.7.7 Normal keys function

The normal functions are accessible by the removable key only. The keys functions on the
keyboard (Pic. 37) are as follows (Tab. 4):
Tab. 4 - User level - Normal keys function of key pad
KEY LED LED

FUNCTION FUNCTION DESCRIPTION MODE
SYMBOL COLOUR STATUS
MODE: MODE 2 selection. START-UP -- --
LIGHT: cabinet lighting ON/OFF. 1 and 2 BLUE FIXED
UV-C light
UV: 2 YELLOW FIXED
activation/programming.
SOCKET: service socket ON/OFF. 1 and 2 BLUE FIXED
Flammable gas line solenoid

GAS: 1 YELLOW FIXED
valve ON/OFF .
UP/DOWN front window
WINDOW: 1 and 2 -- --
motion.
5.1.7.8 Window sash keys
These keys are marked by the symbol (see Pic. 37) and control an electric winder that
allows opening and closure of the frontal access.
The key marked allows raising the window and the key marked allows lowering it.
More information is available in 5.1.3.
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Part 1: User
5.1.7.9 Advanced keys function

Below functions of the keys (Tab. 5) allow the access to information and setting.
Tab. 5 - Advanced function of keys
KEYS
MODE SPECIAL FUNCTION DESCRIPTION
SYMBOL
2s 1 Holding key for 2 s, access/exit ECO MODE. (FW 7.xx)
1 Shows cabinet run hours (hold pressed).

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2 Language selection.
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2 Date and time setting.

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Once you have chosen the special function, press the keys if necessary with the following
meaning (see Tab. 6):
Tab. 6 – Advanced function of keys - link to the display symbols
DISPLAY NONE
ICON
(EXIT) (UP) (DOWN) (ENTER) -
KEY
MODE LIGHT UV SOCKET GAS
INCREASE DECREASE
FUNCTION EXIT ENTER NONE
PARAMETER PARAMETER
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Part 1: User
5.2 OPERATING PROCEDURES
WARNING! Do not use the cabinet devoid of the work surface.
This BSC may operate in two modes:

MODE 1 - SAFE OPERATING;
 Automatic control of the ventilation parameters.
 Alarm devices activation.
 Cabinet lighting.
 Service socket activation.
 Solenoid valve activation (if flammable gas line is installed).
 Run hours control, also partially for maintenance purposes.
 Blackout warning.
 Displayed messages of failure or operator’s wrong actions;
 Reading of running hours (general, UV, filters).
 ECO mode (see 5.2.1.5), only available on cabinets with FW 7.xx.
MODE 2 - STAND BY (UNSAFE);

 Cabinet lighting.
 Moving up and down of the front window.
 Service socket activation.
 Language selection (Italian, English, French, German, Spanish)
 Date and time setting.
 Switching ON/programming of UV light cycle.
NOTE 1: MODE 2 operation is indicated by permanent visual alarm (red LED).
NOTE 2: in MODE 2 audible alarms are disabled.

Both conditions are visualised on the display.
Ventilation control and supervision of the functions are microprocessor based.
MODE 1 (see 5.2.1) is the default operating condition of this BSC without action after turning it ON by the
key switch (see 5.1.7.2).
MODE 2 (see 5.2.2) can be selected within 10 seconds after AUTOTEST only, as display instructs (Pic. 39).
To change operating MODE restart the cabinet by the key switch.

To shut down this BSC turn the key switch to OFF (position O).
See instruction also in 5.2.3
The key of the switch should be always removed (both ON and OFF condition) and kept by the operator in
order to not allow the use of the cabinet by unauthorized persons.
NOTE 3: to turn off the cabinet in an emergency, in the absence of the key, remove the plug of the
power cord (see 2.3.1) from the mains socket (see 2.3.2).
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Part 1: User
5.2.1 MODE 1 ACCESS
 Before activating this mode, carefully read all PART 1 of the manual.
 Before each start, check the correct positioning of the work surface.
1. After checking the correct arrangement of the work surface,

turn the cabinet on by the key switch (see 5.1.7.2).
The system starts showing the firmware revision number and
the AUTOTEST checks (Pic. 38).
If the self-test is passed, the screen is shown (Pic. 39) to
decide within 10 s whether to continue accessing in MODE 1 Pic. 38
(default access without action) or switch to MODE 2 (see
5.2.2).
2. Wait for WARM UP TIME (Pic. 40).
Ventilation started with an audible and visual alarm.
The front window (if totally closed on start-up) is lifted
automatically reaching the working position..
If the front window is not closed, it can only be adjusted at the Pic. 39
end of the WARM UP.
If the WARM UP has been successful, the SAFE OPERATING
screen (Pic. 41) will appear where the vertical air flow velocity
(LAF) and barrier values are shown numerically and
represented by a horizontal bar graph.
The alarm will stop when the correct flow value is reached.
The following are enabled: Pic. 40
up and down front window movement, light, service socket,
gas solenoid valve (if installed) and ECO mode.
3. Remove the key from the switch and keep it in a safe place.
4. If an alarm appears, see 5.2.1.6.
NOTE: read the information about the working chamber (see

5.1.6) and the guidelines (see paragraph 6 and sub-paragraphs).
Pic. 41
5.2.1.1 Lighting ON/OFF
The lighting can be switched ON and OFF via the key . See also 5.1.1.
5.2.1.2 Sockets ON/OFF
The sockets are controlled simultaneously via the key . See also 5.1.2.
5.2.1.3 Solenoid GAS valve ON/OFF
The solenoid valve for use of flammable gas is controlled by the key . See also 5.1.5.2.
5.2.1.4 Run hours meter

1. In MODE 1 (see 5.2.1) press simultaneously and hold
MODE+GAS keys + .
The display will show the run hours from the last zero
setting of the counters (cabinet, filters, UV) (see Pic. 42).
Releasing the keys the display will return to SAFE
OPERATING message. Pic. 42
NOTE: the zero setting can be carried out at SERVICE level only.
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Part 1: User
5.2.1.5 ECO MODE
WARNING! Not safe mode for normal working activity.

During ECO MODE the cabinet cannot be considered safe for working.
For the purpose of warning, during this mode the alarm light (see 5.1.7.3) is on.
 BEFORE activating ECO MODE any sample still lying in the working area must be
put in a safe condition.
This mode saves energy in cases where, without any operator activity (eg a waiting period
between one activity and another), it is necessary to maintain the sterility of the working area
and still guarantee the front barrier.
In this way the return to MODE 1 for a new activity takes place without having to wait for the
start-up procedure.
NOTE 1: this MODE is only available on cabinets with FW 7.xx.
WARNING! Automatic downward movement of the front window.
 Check neither your arms nor objects are across the front aperture while
activating this mode.
NOTE 2: if the front window is jammed, or gets jammed, before or during the activation of
ECO MODE, the cabinet will automatically return to MODE 1 and display the screen of Pic. 48.
1. In MODE 1 (see 5.2.1) press (MODE key) for 2

seconds.
An acoustic and visual alarm is activated to indicate the
automatic movement of the front window downwards to
reduce the height of the front access.
At the same time the airflow will be reduced.
The lighting goes out and cannot be lit. Pic. 43
The solenoid valve is deactivated and cannot be activated.
The service sockets remain in the state as they are.
Once the reduced height of the front access and the set ventilation value have been
reached, the acoustic alarm goes out, while the visual one remains.
The display shows the screen of Pic. 43.
2. To exit ECO mode (restoring of MODE 1), press (MODE key) for 2 seconds.
An acoustic and visual alarm is activated to indicate the automatic movement of the front
window upwards to the work position of the front access.
At the same time the airflow will be increased to reach the configured value.
During MODE 1 restoring the display shows the Pic. 45 with acoustic and visual alarm on
until the cabinet is in operational safety conditions.
The lighting goes on.
The solenoid valve remains deactivated.
The service sockets remain in the state as they are.
Once the operational safety conditions have been reached, the alarm goes out and the
display shows the Pic. 41.
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Part 1: User
5.2.1.6 IRREGULARITIES AND ALARMS
WARNING! Do not use this BSC with alarm activated.
In MODE 1 some warnings or alarms may occur (see from Pic. 44 to Pic. 53).
Pic. 44 Pic. 45 Pic. 46 Pic. 47

Audible and visual Audible and visual Audible and visual Visual alarm.
alarm. alarm. alarm. After a BLACKOUT the
Due to incorrect Due to BARRIER lower Due to LAF out of previous conditions are
position of the front than the minimum allowed limits. automatically restored,
window. allowed. It can appear for a few (EN 12469, H.3.4).
Correct the position of It can appear for a few seconds at the end of Assess whether
the front window using seconds at the end of the WARM UP. BLACKOUT could have
the appropriate keys. the WARM UP or ECO If it persists, shut down compromised the work
MODE. and close the cabinet in progress.
If it persists, shut down and call SERVICE. Press a key to cancel
and close the cabinet the alert.
and call SERVICE.
Pic. 48 Pic. 49 Pic. 50 Pic. 51

See instruction in Damaged real time Corrupt system data. The cabinet needs
5.1.3.1. clock. The cabinet is not calibration.
If it persists, shut down Shut down and close usable. Access in MODE 1 is
and seal the cabinet. the cabinet. Shut down and close disabled.
Call SERVICE. Call SERVICE. the cabinet. Shut down and close
Call SERVICE. the cabinet.
Call SERVICE.
Pic. 52 Pic. 53
Audible and visual Visual alarm.
alarm. If the cabinet suffers a very short voltage dip, it
Due to absence of flow restarts automatically with restoration of the
sensor signal. previous conditions.
Shut down and close Assess whether this may have compromised the
the cabinet. work in progress.
Call SERVICE. Press a key to cancel the warning.
If the problem occurs several times, call
SERVICE..
NOTE: the audible alarm cannot be muted.
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5.2.2 MODE 2 ACCESS
1. Turn the cabinet on by the key switch (see 5.1.7.2).
2. After AUTOTEST (Pic. 38) press the key MODE within 10

seconds as showed in the message of MODE 2 access option
(Pic. 39).
3. Wait for STAND-BY message (Pic. 54).
Pic. 54
Light, service socket and UV light are enabled.
The alarm notices are for the following event:

a. BLACKOUT OCCURRED (Pic. 55) with notice during
restored operating condition;
NOTE 1: the operating conditions are restored prior to

Pic. 55
the blackout.
NOTE 2: press any key to cancel the message.
5.2.2.1 Lighting ON/OFF
The lighting can be switched ON and OFF via the key .

See also 5.1.1.
5.2.2.2 Sockets ON/OFF
The sockets are controlled simultaneously via the key .

See also 5.1.2.
5.2.2.3 Language selection

1. In MODE 2 (see 5.2.2) press simultaneously MODE+LIGHT
keys + .
The display will show the language menu (Pic. 56).
2. Select the language by pressing the keys.
3. Press key to confirm or press key to exit without Pic. 56
saving.
5.2.2.4 Date and time setting

1. In MODE 2 (see 5.2.2) press simultaneously MODE+UV keys
+ .
The display will show the date/time set up menu (Pic. 57).
2. Select data to modify by pressing the keys.
3. Press key to enable the modification of the data. Pic. 57
On the left of the selected data will appear an arrow.
4. Modify the value by pressing the keys.
5. Press to save and exit or press to abort and exit.
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5.2.2.5 Activation and programming of UV-C cycle
WARNING! See ASSESSMENT OF RISKS, warning from 1.7 to 1.8

 See further information in paragraph 5.1.4
After the cleaning of working area surfaces was done (see 7.1.2), it is possible to sterilize
them by radiations of this lamp.
To start or to program a cycle of UV decontamination, proceed
as follows:
1. Turn on the cabinet, and enter MODE 2 (see 5.2.2).

2. Close the front window.
3. Check fluorescent light be OFF.
Pic. 58
4. Press UV key .
Display will show the message of Pic. 58.
5. Select the UV cycle time by pressing the key (UV).
Default setting is 10 minutes: press again repeatedly the key
to increase the cycle time, with 10 minutes steps, till the
required value is reached, in the range 10 to 60 minutes.
6. Press key (SOCKET) to confirm. Pic. 59
Display will show the message of Pic. 59.
Current time is proposed as cycle start.
Press key to start the UV cycle immediately (skip to
point 8), or repeatedly press the key to program the
starting time.
Programming range: within next 24 hours.
7. Press key to confirm. Pic. 60
Display will show the message of Pic. 60 with the
programmed activation time.
9. At the programmed time, the UV lamp will light up.
Display will show the message of Pic. 61 (remaining
activation time).
10. When the decontamination cycle is completed the UV-C lamp Pic. 61
turns OFF and display will show the message of Pic. 62.
11. Press key (LIGHT) to exit and return to MODE 2 operating
message (Pic. 54).
NOTE: UV lamp can be activated only when the front window

is fully closed and the fluorescent light is OFF.
Pic. 62
5.2.3 SHUTDOWN
5.2.3.1 Operational shutdown

This BSC can be turned off from MODE 1 and MODE 2 exclusively via the key switch (see
5.1.7.2).
This operation does not guarantee disconnection from the mains (see 5.2.3.2).
1. Turn the switch to position O.
5.2.3.2 Disconnection from mains

The means of disconnection from the mains is the power cord plug.
Remove the power cord plug from the mains socket.
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Part 1: User
6. GENERAL RULES – GUIDELINES
 WARNING! See ASSESSMENT OF RISKS, warnings from 1.1 to 1.15
Proper housekeeping and physical leaning of the facility is important for maintaining safe working
conditions for personnel, product and the surrounding environment.
Cleaning and sanitizing shall be carried out by trained personnel in accordance with specific, written, and
approved procedures (SOPs).
6.1 FEATURES OF LOCATION:

 The location of the workstation must comply with the indications in 2.1.
 This BSC must be equipped with an efficiency test report, to be issued at the installation and
updated every 6 months, or in the event of replacement of the filters or other components of the
cabinet and in case of repositioning or dislocation.
 Chair should assure fully support of the body.
 If the BSC is used by several operators, use an adjustable chair to make it adaptable to the various
operators.
 Avoid placing objects that limit the legroom under the BSC.
6.2 WORKING POSTURE:

 If the chair has adjustable low back support, match the chair backrest to support the lower part of
the spine.
 Keep forearms, wrists and hands aligned in a straight neutral position: avoid bending or angling the
wrist;
 Working intensely for long time poses risks: vary the leg position frequently and get up from the
chair periodically taking brief walks.
6.3 WORKING PROCEDURES:

 Make sure the particular BSC is suitable for your work (risk assessment).
 Check that the efficiency test report of the BSC is up to date.
 Operate this BSC within the limits described in DEFINITION OF IMPROPER USE, CABINET
DEFINITION and NOT ALLOWED USE.
 DO NOT use this BSC as material storage.
 Organise the work according to a detailed plane in which working steps, needed materials and
accessories (to be put in the cabinet) are listed.
 Put inside the cabinet only essential materials and equipment, before operating the cabinet, so that
nothing would pass through the front aperture during operation.
 Use this BSC only if equipped with the correctly inserted work surface.
 Verify that the work surface is placed correctly (see 5.1.6.1).
 Verify that there are no obstructions to the exhaust air outlet before turning the cabinet on.
 Wait at least 10 minutes of SAFE OPERATING condition in MODE 1 (see 5.2.1) of the cabinet before
starting any operation, in order to allow the removal of the airborne contamination.
 Wash hands and arms always with a germicidal soap before and after work.
 Put on the appropriate protective clothing according to the level of containment and the risk
assessment for the work (e.g. long sleeves gowns with elastic cuffs, and rubber gloves).
 Put objects at least 15 cm off from the front window and operate in the innermost part of the safe
working area (see 5.1.6.2).
 Do not let other people in the laboratory intrude in your space: keep at least 1 metre clear behind.
 Do not put any paperwork in the BSC.
 Do not rely on this BSC to cover up poor technique.
 Avoid sudden and sweeping movement of the arms to minimise disturbance of the airflow patterns.
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 Avoid that contaminated material passes over the sterile one when shifting it; keep contaminated
material separate from the sterile one, place the latter far from the working area and put the wastes
in a back corner of the working surface.
 Do not place large or bulky equipment in this BSC and do not place equipment on ventilation grilles
to avoid disturbing airflow patterns.
 Don’t obstruct the slots of the whole working chamber
(see 5.1.6.3) with objects (see Pic. 63) and especially
careful not to obstruct the front intake grille with
sleeves of the working garment.
 Use this BSC only when the ventilation is operating
(MODE 1), with functioning display and in the absence
of alarm (acoustic or visual).
 Limit the activities inside the room where the cabinet is
placed, because they could disturb the air barrier
containment and the laminar flow (LAF), with
consequent decrease in the operator and product level
of protection.
Each time doors of the laboratory are open and closed,
drafts are produced.
Pic. 63
 Repeat the decontamination of inside surfaces once all
objects as materials and equipment have been taken out of the cabinet.
Pay special attention when removing cultures that have been accidentally poured inside the working
area: they could favour the growth of fungi and the spreading of their spores with consequent
contamination of the cabinet. For cleansing and decontamination, see par. 7.
 Keep the cabinet on for at least 10 minutes once the work is over, in order to remove any
contaminating substance from inside.
 Subject this BSC to routine checks as indicated by the manufacturer (see 10.1.1) or by regulatory
authorities, if more restrictive.
7. CLEANSING AND DECONTAMINATION
7.1 CLEANSING
7.1.1 Painted surfaces

 Use common detergents, like soap solutions.
 DO NOT USE solvents or other paint aggressive products.
7.1.2 Stainless steel surfaces

 Specific products are recommended for normal cleaning.
 The disinfection of the inner surfaces of the working space shall be carried out by using
disinfectant liquids, following the relating instructions to prevent misuse.
Decontaminate by swabbing with a disinfectant chlorine-free product.
WARNING!
Chlorine products damage stainless steel surfaces.
7.1.3 Glass surfaces

 The cleaning and disinfection of the glass window should preferable be carried out with
alcoholic disinfectant as isopropyl alcohol.
NOTE: to clean the inner area of the front window which is not normally accessible,
follow the procedure described in 7.1.3.1.
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7.1.3.1 Complete cleaning of the front window
 Where highlighted, two operators are required.
Pic. 64 Pic. 65
1. In MODE 2 (see 5.2.2) make the lower edge (Pic. 64, items 1) of the front window
coincide with the point marked by a notch on the side cover (Pic. 64, item 2).
2. Disconnect the cabinet from the mains (see

5.2.3.2).
3. Unscrew the control panel locking screws (Pic. 65,
item 1) with the dedicated key (Pic. 33).
See warning in 5.1.7.1.
4. Raise the control panel slightly pulling it

towards you using the handle (Pic. 65, item 2) in
order to allow the side covers to be removed Pic. 66
upwards (Pic. 66 – items 2 and 3).
In this way, the struts (Pic. 65 – item 1) needed to
fix the window in a raised position suitable for
cleaning will be accessible.
5. Gently place the control panel back on the
structure.
6. Lift the front window by pulling the lower

edge towards you (Pic. 67 – item 2) until the struts
can be inserted between the window and the
cabinet (Pic. 68 – items 2 and 3), as shown in Pic.
68 – item 1.
Pic. 67
NOTE: the control panel will follow the lifting
leaning on the window frame.
7. Release the window gently making sure

it is firmly held lifted by the struts.
Pic. 68
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8. Proceed with cleaning as shown in Pic. 69.
NOTE: use the products indicated in 7.1.3
9. After cleaning, lift the front window by

pulling the lower edge towards you until the struts
can be removed (Pic. 67 – item 2).
10. Gently release the window to rest it on the

structure.
11. Refit the struts in their dedicated locations.
12. Slightly lift the control panel using the

handle (Pic. 65, item 2) in order to allow the re-
insertion of the side casings.
13. Gently release the control panel to rest it

on the structure.
14. Tighten the locking screws (Pic. 65, item 1) with the
dedicated key (Pic. 33).
Pic. 69
15. Reconnect the cabinet to the mains and proceed with normal use.
16. Return the control panel release key to the laboratory manager.
7.1.4 Equalizer fabric of LAF filter
The exposed face of the laminar flow filter inside the work chamber is covered with a sheet of
micro-perforated polyester fabric with a surface treatment that has the task of making the
laminar flow as uniform as possible.
In case of splash contamination on this fabric, decontamination of the surface is possible using
isopropyl alcohol.
WARNING!
Carrying out this operation without care can damage the filter and compromise
product safety.
 Perform this operation only if strictly necessary, in the event of an accident that
has caused splashes of contaminated substance on the fabric, very carefully and
gently.
Procedure:
with a soft piece soaked in isopropyl alcohol, gently rub the face of the filter without pressing on
the protective grille underneath.
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7.2 DAILY DECONTAMINATION

Routine decontamination of the working surfaces can be performed by means of UV-C radiation (see
5.1.4).
WARNING!
See ASSESSMENT OF RISKS, warnings 1.7 and 1.8
See 5.1.4.1
WARNING!
The procedure by UV-C radiation does NOT guarantee a real reduction of microbial
contamination, as it is effective only for the surfaces directly exposed to the radiation.
This method does NOT replace the conventional ones of decontamination of a BSC.
In the following cases use a valid decontamination method:
 before accessing to any potentially contaminated area;
 every time big spillage of contaminated liquids occurs;
 before maintenance (fan and filters replacement);
 before routine control;
 before dislocation or decommissioning;
 after any emergency.
 The operator must identify the effective decontamination method against the pathogen in
question.
7.3 BIOLOGICAL DECONTAMINATION

In PART 2 of this manual a validated method is proposed (see # 9).
The operator must verify the adequacy of the proposed method through a risk assessment for
the products handled in the cabinet.
Decontamination must be carried out by trained and authorized technical personnel.
7.4 LAMPS REPLACEMENT

Replacement procedures are indicated in 7.4.1 and 7.4.2.
WARNING!
The breaking of UV and fluorescent lamps pose a risk of injury to the skin and eyes, as well as
pose a risk to health due to mercury vapour, metal contained in the lamps even if in small
quantities, which may be released into the environment.
 Before proceeding with this operation, it is essential to strictly respect the following
instructions, in order to protect yourself and always be in safe conditions.
NOTE: also consider that the space in which you operate is always subject to
contamination.
For these reasons the replacement of the lamps is recommended to be carried out by
technical service and maintenance personnel.
Before replacing proceed as follows:
 decontaminate the work chamber;

 switch OFF the BSC and disconnect the power cord plug from the mains
socket;
 always use protective gloves, goggles and mask.
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Part 1: User
7.4.1 UV LAMP
Turn off the BSC and disconnect it from the mains (see 5.2.3.2).
1. To replace this lamp grasp the

tube at the ends and rotate it 90
degrees down or up (Pic. 70-item
1).
2. Pull the lamp (Pic. 70-item 2)
towards you by sliding the tines
out of the slots of the lamp
holders (Pic. 70-item 3).
3. Decontaminate the lamp.
4. Rest the lamp gently in a safe
place where it cannot roll or risk
falling and breaking, waiting for
disposal.
Pic. 70
5. Place the new lamp by inserting the tines into the slots of the lamp holders and rotating the
tube 90 degrees down or up.
6. Start the disposal procedure as indicated in 7.4.4.
NOTE: in case of breakage of a lamp refer to 7.4.3.
7.4.2 FLUORESCENT LAMPS
Electric shock hazard.
1. To replace these lamps, unscrew the front box-like

panel locking screw (Pic. 65, item 1) with the
dedicated key (Pic. 33).
2. Disconnect the cabinet from the mains (see

5.2.3.2).
3. Raise the control panel slightly pulling it

towards you (Pic. 71, item 1) using the handle (Pic.
71, item 2) and keep it open enough to reach the
lamps.
The lamps are applied in the position shown in Pic.
71 – item 3.
4. Proceed to remove the lamps in the same way as
indicated in 7.4.1.
Pic. 71
5. Rest the lamps gently in a safe place where it cannot roll or risk falling and breaking, waiting
for disposal.
6. Place the new lamps in the same way as indicated in 7.4.1.
7. Gently release the control panel to rest it on the structure.

8. Tighten the locking screws (Pic. 65, item 1) with the dedicated key (Pic. 33).
9. Return the control panel release key to the laboratory manager.
10. Reconnect the cabinet to the mains and proceed with normal use.
11. Start the disposal procedure of the lamps as indicated in 7.4.4.
NOTE: in case of breakage of a lamp refer to 7.4.3.
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7.4.3 IN CASE OF BREAKAGE OF LAMPS

Although it is extremely unlikely that lamp breaking could have any impact on the user
health, in the event of breakage, follow the instructions below:
 assess the contamination that the lamp may have undergone to establish the correct
disposal procedure;
 wear adequate PPE or the assessment made, ventilate the room for 30 minutes
(switch off any conditioning systems) and gently remove the fragments collecting
them with rigid elements such as cardboard sheets;
 clean items or clothing that come into contact with dust coming out of the tube using
paper towels or wet rags;
 do not wash these clothes in the washing machine;
 do not use vacuum cleaners or brooms to avoid dispersing mercury particles in the
environment;
 store the broken parts and everything that has been used for removal and cleaning in
a sealable plastic container and dispose of them according to the local regulations in
force.
7.4.4 DISPOSAL OF LAMPS

The removed lams must be sealed and identified as a HAZARDOUS WASTE.
Therefore they must be treated by companies qualified and authorised for the disposal of
such waste.
 Use sealable plastic containers suitable for temporary storage.
 Identify the container with appropriate label showing the type of waste.
 Start the correct disposal procedures according to the local ruling regulations.
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PART 2:
INSTALLATION
AND
MAINTENANCE
INFORMATION FOR TRAINED AND AUTHORIZED SERVICE STAFF ONLY
WARNING!
 All the operations described in this part of the manual should be performed only by
qualified technicians, properly trained authorized.
 The user is not allowed in any of these operations.
BioAir declines all the responsibility for damage resulting from non-compliance with these provisions.
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Part 2: INSTALLATION AND MAINTENANCE
8. INSTALLATION
WARNING!
All operations described in this Part 2 of the Manual should be performed by specialized and
authorised personnel, in compliance with the ruling laws concerning safety at work and
using all the necessary individual and collective protection.
Always consult the safety data sheets of products used for the operations described in this
manual.
WARNING!
Check the environment in which this cabinet can be installed (see paragraphs 2and 3.4).
MANIFESTED AND FOR DAMAGE TO PERSONS AND/OR THINGS DUE TO NON-AUTHORISED
INSTALLATIONS AND/OR WHICH ARE NOT COMPLIANT TO THE OPERATING INSTRUCTIONS
CONTAINED IN THIS MANUAL.
THE SAFETY OF ANY SYSTEM INCORPORATING AND/OR INTEGRATES THIS CABINET IS THE
RESPONSIBILITY OF THE ASSMBLER OF THE SYSTEM.
Pic. A1
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8.1 HANDLING AND UNPACKING

WARNING!
Most damages to the cabinets occur during this phase.
The BSC is shipped on a dedicated pallet with protective wrapping film and outer cardboard.
To handle and lift the cabinet is necessary to use a hydraulic fork-lift truck (trans-pallet) with
lifting forks up to a height of about 1 m. The length of the forks should be at least 80 cm.
Check that the fork truck is adequate for the weight of the cabinet (see 3.6).
Remove the packing material only when the pallet is safely placed on the floor.
8.2 POSITIONING
WARNING!
Not suitable place may compromises the performance and the biological safety of the cabinet.
Read information in PART 1 (see the entire paragraph 2.1).
The installation place should meet the following provisions:

 air-draft-free room to avoid interference with the air barrier containment;
 no air conditioning louvers close to the cabinet;
 no aisles affected by foot traffic;
 an available near-based grounding mains socket (see 8.5);
 at least 500 mm of space to the cabinet sides for technical access;
 at least 650 mm of space in front of the cabinet; and
 at least 300 mm of space between the exhaust air outlet and the room ceiling.
The overall dimensions of the cabinet mounted on the floor stand are shown in Pic. A1.
The cabinet is to be placed on the floor stand.
WARNING!
Not suitable support stand may compromise the stability of the cabinet.
Use of different support is strongly advised against and it is under total responsibility of the
customer.
 Fit the cabinet on the support stand available in the BioAir optional accessories list.
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8.3 INSTALLATION OF OPTIONAL EQUIPMENT
THE SAFETY OF THE BSC DEPENDS ON THE CORRECT INSTALLATION OF THE ACCESSORIES
ACCORDING TO WHAT IS INDICATED IN THIS MANUAL AND IT IS THE RESPONSIBILITY OF THE
ASSMBLER.
8.3.1 FLOOR STAND
8.3.1.1 Stand assembling
NOTE: the floor stand (Pic. A2) is supplied

disassembled in two alternative ways:
 Fixed height.
 Adjustable height.
Assembling instructions are included within the

stand packing.
Follow those instructions for a correct assembly.
Pic. A2
WARNING!
Before assembling of the adjustable stand, check the height restrictions for this
cabinet model (see Part 1, 2.2.1).
8.3.1.2 Placing of the cabinet on the stand
 At least 2 trained operators are required.
1. Prepare the stand according to assembling

instructions (see 8.3.1.1) deciding which
working height you want to use.
2. Put the forks of the truck in front of the pallet
and raise the forks at the same level of the
cabinet bottom surface.
3. Ensure the forks are properly aligned with the
pallet base (Pic. A3 – item 1), then slide the
cabinet on the forks (Pic. A3 - item2).
The cabinet bottom is purposely reinforced to
cope with this procedure. Pic. A3
Follow the basic ergonomic principles
when sliding the cabinet onto the forks.
4. Make sure the forks are positioned so as to

maintain the load stable and balanced.
5. Lift the cabinet up to ~ 1 cm above the stand
height (Pic. A4), then proceed to an accurate
alignment of the four pass-through holes with
the M8 threaded inserts at both sides (2 on
the right and 2 on the left) of the cabinet (Pic.
A5).
Never lift the load more than necessary.
Pic. A4
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6. Lift a little the stand (Pic. A5

– item 1) and fit through
the holes of the stand
frame, not tight screwing,
no. 4 screws M8x16, with
relevant washers in the
inserts of the cabinet (Pic.
A5 – item 2).
7. Carefully rest the cabinet
and stand on the floor,
tighten the screws, lower
the forks and remove the lift
truck.
8. Level the cabinet.
Use the adjustable feet of
the floor stand.
Pic. A5
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8.3.2 ASSEMBLING OF GASES AND FLUIDS TAPS

WARNING!
 The installation should be carried out in accordance with the ruling standards and
instructions supplied with the accessory.
1. Unscrew the two grub screws (Pic. A7 – items A and

B) and remove the protective plugs (Pic. A7 – item C)
from the connections dedicated for taps inside the
working chamber (Pic. A6 – items 1 and 2).
Use an Allen key No. 2,5.
2. Fit the taps by inserting the bayonet (see Pic. A8) into
the appropriate connection.
Be careful to connect the correct line (check the
identifying adhesive labels).
3. Retighten the safety grub screws.
4. The taps assembled are showed as in Pic. A9.
5. Install the solenoid valve on the flammable gas line
(see 8.6.2).
Pic. A6
6. Check the tightness of the circuits according to the
instructions given in 8.3.2.1.
Pic. A7 Pic. A8 Pic. A9
8.3.2.1 Testing of gas circuits tightness

The GAS LINES test should be performed with a pneumatic test, partially referring to the
paragraphs 11.7.2 and 11.7.3 of the standard IEC 61010-1.
Pic. A10 shows the test in a schematic way.
LEGEND
1. Gas tap.
2. Solenoid valve (or hose barb).
3. Connection pipe for the test.
4. Pressure test tap.
5. Pressure test manometer.
6. Test pressure.
Pic. A10
1. Switch on the cabinet in MODE 1, close the taps inside the cabinet and supply the solenoid
valve.
2. Supply pressure gradually through the test tap (Pic. A10 – item 4) until a pressure is read on
the pressure gauge with the following correction factors:
 2,0 times the nominal operating pressure for the flammable gas line (see 3.10.3);
 1,5 times the nominal operating pressure for the technical gases line (see 3.10.2).
NOTE 1: avoid providing higher pressures so as not to stress the circuits.
NOTE 2: use pressure gauges with reading range consistent with the used pressures.
3. Close the test tap and wait for at least 1 minute.
TEST RESULT: no leakage must be found.
NOTE 3: to connect to the gas lines go to paragraph 8.6.
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8.3.3 Ducting of exhausted air flow
The exhaust air can be left free in the environment, but in the required cases can be further
filtered and/or ducted outside.
The BSC shall not be connected directly to any other air-handling system.
NOTE: it may be provided exhaust the air to the atmosphere through an indirect connection,
and possibly to further purify the exhaust air, e.g. through an absorbent filter.
These modifications can be obtained through the installation of accessories designed by BioAir for
these needs. Below are the available accessories:
WARNING!
The requirement and feasibility of this type of modifications should be carefully
assessed by competent personnel in order not to compromise the performance of the
cabinet together with the safety of the operator, the environment and the product:
 in the case of an external ducting, an adequate air intake must be guaranteed in
the room;
 in the case of an additional filter, the increase in pressure drop on the exhaust
must be compensated by an adequate and balanced additional suction.
 Read also information in PART 1 of this manual (see 2.5).
 ACTIVE EXHAUST KIT:

motorized box with electronic regulation of the flow rate, suitable for balancing pressure drop
due to additional exhaust filters (absolute filter or activated carbon filter) or due to small
sealed ducts (less than 10 m).
Since the duct is under positive pressure, it is advisable it be inspectable for leakage checks.
It is activated directly from the cabinet.
 PASSIVE EXHAUST KIT:
non-motorized box to be combined with BioAir REMOTE EXTRACTION KIT (for ducting up to 18
m ) or remote extraction systems whose flow rate can be adjusted.
It is suitable for ducting by means of a sealed connection or temporary ducts for the purpose
of expelling the residues of substances used for decontaminating the cabinet.
 PASSIVE EXHAUST OPEN KIT (THIMBLE):
non-motorized box to be combined with BioAir REMOTE EXTRACTION KIT or with remote
extraction systems that are also not adjustable in flow rate.
It is suitable for ducting via an open connection.
This type of accessory offers several advantages over others:
 allows the use of extraction systems that have an airflow rate greater than that
required and which can not be reduced;
 does not involve imbalances on the cabinet airflows;
 faults on the extraction system do not affect the performance of the cabinet;
 any problems with the air intake of the room have no effect on the performance of the
cabinet.
These accessories allow ducting the air exhausted from the cabinet into round section ducts of
200 mm diameter. They are installed on the top of the cabinet where the fixing points are
located. It is not recommended to reduce the ducting diameter.
For this type of requirements contact BioAir, which, directly or through local distributors, will
provide assistance for the specific needs of the laboratories, proposing the appropriate
solutions.
NOTE: the installation of these accessories must be carried out after the cabinet has been
tested, as the cabinet performance must first be checked in normal conditions. Subsequently
the validation must be made with the new configuration, carrying out again the tests related
to the values of the airflows and to the alarm interventions.
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8.3.4 VFC output (auxiliary contact)

The connector shown in Pic. A11 provides a normally open volt-free contact for
the control of remote devices or for status signalling.
It is located on the right side of the upper part of the control panel (see Pic. 15
- item 3, par. 5.1.7.4 and Pic. 36 - item 3).
Features and limits of use are available in 3.9.2. Pic. A11
It can be configured as FAN ON or as ALARM ON (see 10.1.4).
To use it, prepare the movable connector
(Pic. A12 - item 2) with a 2 poles cable
connected to terminals L and N (Pic. A12 -
item 3).
Connect the other end of the cable to the
external circuit that you want to control.
Insert the connector into the fixed connector
on the cabinet (Pic. A12 - item 1).
Pic. A12
8.3.5 Alarm mute inlet
This input allows you to silence the alarm during installation tests and routine
checks.
It is located on the right side of the upper part of the control panel (see Pic. 15 item
3, par. 5.1.7.4 and Pic. 36 item 4).
The mute takes place by inserting the tool to be requested from BioAir (see Pic.
A13). Pic. A13
WARNING!
 Alarm mute is not allowed during the normal use of the cabinet.
Remove the tool immediately once the tests are finished.
8.3.6 Data output port
Optional, factory installation only.

Data output (RS232) of operational parameters, status of the services (light, service socket, UV)
and eventual alarms.
It is placed on the right-hand top side of the front panel (Pic. 36 - item 5).
NOTE: communication software is not supplied.
8.4 DISPOSAL OF THE PACKAGING

The packaging must be disposed in compliance with laws and regulations in force in the country where the
cabinet is installed.
 Collect the packaging and disposed of it separately in compliance with any law and
regulation in force in the country where the machine is installed.
 DO NOT dispose of plastic film wrapping as unsorted municipal waste.
 Any plastic material is to be collected and disposed of separately in compliance to laws

and regulations in force in the country where the machine is installed.
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8.5 CONNECTION TO THE MAINS
WARNING!
 Read information in PART 1 of this manual (see the entire paragraph 2.3).
To proceed with the connection, follow the instructions below:
 Check the type of power supply required by the cabinet (see 3.1) and the
environmental conditions of use (see 3.4).
 Verify that the supplied power cord is consistent with the assigned mains power
socket. The features of the cable are indicated in 2.3.1.
 Use a dedicated power line with an electrical socket compliant with the specified in
2.3.2 and 8.5.1.
 The supply cord (detachable) must be connected as indicated in 2.3.1.
 Main fuses specifications are available in 2.3.3.
PROTECTION DEVICE FOR THE ELECTRIC LINE
 The tripping curve of the circuit breaker of the power line should be of type C.
SUPPLY BY UNINTERRUPTIBLE POWER SUPPLY (UPS)
 If the power supply is supplied by UPS, the generated waveform must be pure
sinusoidal type.
WARNING!
 Given the large number of models on the market, the operation of the cabinet can not be
guaranteed with all sine waveform generation systems.
Contact BioAir, either directly or through local distributors, to receive information on the
solution of this type of problem.
NOTE: the EFFICIENCY OF THE PROTECTIVE CABLE (EARTH) and DIELECTRIC STRENGTH are checked
in the factory on 100% of the cabinets, according to Annex F of the IEC 61010-1:2010/A1:2016 standard.
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8.5.1 Mains socket

The socket to connect the cabinet should be near-based (max 2 m), harmonized, with features
indicated in Tab. 7 and provided with the ground in accordance with local regulations. The
following conditions must also be verified:
 accessibility to the socket must not taken place directly from the working position, in
order to prevent the cabinet from being accidentally disconnected during use;
 access to the socket must be free from objects or furniture to ensure a quick
disconnection of the cabinet from the mains in the event of an emergency;
 the voltage indicated on the data plate of the cabinet must be consistent with the
mains voltage.
Tab. 7 – Mains socket specifications

Type: single phase, P+N+E
Current (A): 10
Voltage (V): 250
Conformity: AS/NZS 3112
Approvals: Australian
8.5.2 Mains supply cord

The mains supply cord must meet the requirements of 2.3.1 and Tab. 8.
To connect the cabinet read the assembly instructions in 2.3 where it is explained how to fix the
connector to the power inlet by means of the special tool supplied (Pic. 6).
For this purpose the connector must have an angle shape (90 °).
Tab. 8 – Mains supply cord specifications

Type of cable: H05VV-F
Numbers of wires: 3
2
Section (mm ): 1,5
Max length (m): 3
Cable conformity: IEC 60799
Type of connector: IEC320 – C13 angled

Mains connector conformity: IEC 60320-1
Type of plug: I (10 A rated)

Mains plug conformity: AS/NZS 3112
Approvals: European - Australian
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8.6 CONNECTION TO THE GAS LINES (OPTIONAL)
Read the assembly instructions supplied with the

accessory to be installed.
Inlet hoses are located at the top of the rear right-hand
(Pic. 15 – items 14 and 15) and must be marked with
suitable label (see 5.1.5).
The flammable gas line is conventionally connected to
the item 2, in order to keep the passage hole (Pic. A14
- item 3) of the solenoid valve power cable close by.
The assembly of the taps inside the work chamber is
described in in 8.3.2.
Before connecting the gas lines, carry out the tightness
test (see 8.3.2.1). Pic. A14
The gas lines must be connected in compliance with the regulations in force in your country.
8.6.1 Vacuum/Inert gases line

The operating range and the technical features
are defined in the table DATA SHEET (see
3.10.2).
Connect the INERT / VACUUM GAS line to the
connection indicated in Pic. A14 - pos. 1.
8.6.2 Flammable gas line

The operating range and the technical features
are defined in the table DATA SHEET (see
3.10.3).
Install on the connection shown in Pic. A14 -
item 2 the normally closed solenoid valve (Pic.
A15 - item 3) supplied with the assembly KIT.
Pic. A15
See RISK ASSESSMENT, warning 1.12.
The FLAMMABLE GAS line must be fitted to the inlet connection of the solenoid valve indicated in
Pic. A15 - item 2.
To electrically connect the solenoid valve, proceed as follows:
1. remove the technical compartment cover (Pic. A15 - pos. 6) by unscrewing the fixing
nuts (Pic. A15 - item 7);
2. fit the cable gland (Pic. A15 - item 5) in the provided hole (Pic. A14 - item 3);
3. connect the connector of the cable supplied (Pic. A15 - item 4) for the solenoid valve;
4. insert the free end of the cable supplied into the cable gland (Pic. A15 - item 5) and
connect it to the terminals indicated in the accessory instructions;
5. close the technical compartment and tighten the fixing nuts.
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8.7 PERFORMANCE TEST

WARNING!
 Use instruments calibrated and certificated recently (no more than 1 year), adequate to the
measurements to be performed (full scale, accuracy and precision).
 The instrument calibration certificate must include values consistent with the measurements
to be performed.
The necessary checks are the following and must be performed in MODE 1 (see PART 1, 5.2.1), except
for the alarm thresholds, to be performed in MANUAL MODE (see 8.9.8):
 HEPA filters integrity test (see 8.8).
 Inflow air velocity test (see 8.9.1).
 Downflow air (LAF) velocity test (see 8.9.2).
 Containment at the front aperture (see 8.9.3).
 Smoke pattern test (see 8.9.4).
 Alarm thresholds test (see 8.9.7).
 Working area air cleanliness test (see 8.9.6).
(NOT REQUIRED by the product standard, optional on prior request only)
NOTE 1: the test procedures should be carried out in accordance with the conditions defined
in the factory test report. The results of the laminar and exhaust air flow rate, should be
consistent to the ones of the factory test report (Quality Pass), where the performance data and
the type of instruments are indicated.
NOTE 2: in addition to the instruments required for the checks (see specific paragraphs), it is
advisable to have the following instruments
ADVISED ADDITIONAL INSTRUMENTS

 digital multimeter capable of measuring true RMS AC voltage;
 digital multimeter with a frequency reading.
8.8 FILTERS INTEGRITY TEST (DOP TEST)
8.8.1 Exhaust and LAF filters testing

Method: aerosol challenge.
Reference standard: EN 12469, 7.5, annex D; ISO 14644-3; manufacturer’s recommendations.
(see also AS 1807.6)
Instruments: cold aerosol generator and photometer or discrete particle counter.
The filters should be tested individually.
The test is based on the comparison between the particles detected upstream and downstream the
filter.
In order for the test to be valid, it is necessary that the concentration upstream of the filters is
sufficiently high to have a statistical confirmation. For this reason, particles having a particle size
within a given diameter must be produced by aerosol (see Tab. 9).
Proceed as follows:
1. Switch on the cabinet in MODE 1 (see PART 1, 5.2.1)
or in MANUAL mode (see 8.9.8) and wait a few
minutes for the operation of the cabinet to stabilize.
2. Depending on the instrument you chose to use (see
Tab. 9), connect the probe of the photometer or
particle counter (see 2) to the port for sampling

the concentration of particulate upstream of the filter
(100%), consisting of the rubber tube indicated in Pic. A16
Pic. A16 under the left sector.
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3. Through an appropriate instrument, generate an amount of aerosol in the working area in

order to have a concentration of particles upstream of the filter according to Tab. 9.
The oils that can be used for aerosols must produce particles with a diameter of less than 1
μm (MPPS).
Below is a list of oils that meet the requirements:
 PAO (Poly-Alpha-Olefin)  DOS (Dioktylsebazat)
 DEHS (Di-2-Ethyl-Hexyl-Sebazat)  Shell (Mineraloil)
 PSL (Polystyrol-Latex)  Paraffinoil
The aerosol generated is draw in by the fan and routed to the plenum upstream of the filters
where the required particle concentration has to be reached.
Tab. 9
UPSTREAM
PARTICLES DOWNSTREAM
SCANNING
DIAMETER
DISTANCE
DILUTION
VELOCITY
PARTICLES
PARTICLES
FACTOR
FILTER
CONCENTRATIO
FROM
N LOCAL
PENTRATION
(100 %)
Fotometer 10 ÷ 80 µg/l - ≤ 0.01%
< 1 µm
≥ 1,000,000 1:100 0.05 m/s 3 cm
Particle counter (MPPS) ≤ 0.05%
particles/ft3 see 2
NOTE 2: using a particle counter, to avoid a coincidental error or a damage of the optical
sensor you have to use a dilutor with a suitable dilution factor. The ratio indicated in Tab. 9 is
by way of example.
Check on the particle counter manual which is the maximum countable number of particles /
ft³.
4. Use the photometer or particle probe to scan the whole surface downstream the filters, at the
distance and at the velocity indicated in Tab. 9.
Acceptance criteria: see Tab. 9.
8.9 AIRFLOWS TEST

At the installation, every routine control, and during the calibration, it should be carried out the check of
airflows parameters.
It is advised to use anemometers with accuracy appropriate to the type of measurement to be done and
complying with what indicated in the WARNING notice in 8.7.
The test must be performed with the cabinet in MODE 1 (see 5.2.1), with front window in working position
and activated lighting.
NOTE: any problems of airflow instability, often due to airflow sensor malfunction, can be identified by
proceeding with the check in MANUAL mode (see 8.9.8).
The procedures are described in 8.9.1, 8.9.2, 8.9.3 and 8.9.7.
Before starting the test check the following:
 No presence of external air turbulences due, for example, to open windows, to the air-conditioning
of the room or due to passages of people.
 Correct inserting of the working surface (see 5.1.6).
 The UV lamp must be installed.
 Cabinet lighting must be activated (at maximum power if adjustable).
 Wait about 10 minutes before starting the check.
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8.9.1 INFLOW air:

Method: indirect by exhaust air velocity measurement .
Reference standard: EN 12469, annex G.3.2.2, H.1, H.3.3; manufacturer’s recommendations.
Instruments: 100 mm vane anemometer.
NOTE 1: typical accuracy: ±1% of reading or ±0,02 m/s, whichever is greater).

The anemometer must be supported by a appropriate support so that it can be left in place
without the operator's arm interfering with the measurement.
INFLOW air velocity (VINF) is the mean velocity of the inward air flow at the front aperture which
protects the user (FRONT BARRIER).
The value is obtained by calculation starting from the

EXHAUST air velocity measurement (VEXH) as per EN
12469, annex G.
Check the conditions indicated in 8.9.
Perform this measurement by means of a  100 mm
vane anemometer (see features in 1) in a horizontal

plane grid, on the exhaust filter face (see Pic. A17).
The position of the measurements should be allocated on
columns and rows both equispaced.
Follow the table Tab. 10 to determine the number of
samples to carry out.
The external points of the grid coincide with the
perimeter of the area in which the measurements are
made, except where it is set a single column, which
should be in the middle of measurement area.
The total area shown in Pic. A17 represents the net
surface of the filter. Pic. A17
Tab. 10 – Exhaust measurement grid and calculation factor for INFLOW (KI)
KI (see 2)
CABINET SIZE ROWS COLUMNS

standard ducted
exhaust exhaust
d= 200 mm
0.9 2 1 1,0173 0,1780
1.2 2 2 1,0292 0,1314
MODE 1
1.5 2 3 1,0344 0,1041
1.8 2 4 1,0392 0,0862
NOTE 2: the KI factor is obtained by dividing the area of the section in which the
measurement is performed for the cross-sectional area of the front opening (BARRIER AREA, see
PART 1, 3.6). Tab. 10 lists the KI values according to the sizes and exhaust sections provided for
the cabinet and for accessories with 200 mm diameter ducting.
In case of ducting with a different section, recalculate the KI factor.
1. Calculate the mean of the measurements carried out at the exhaust air outlet (VEXH).
2. Calculate the mean velocity of the frontal BARRIER (VINF) by the following formula:
VINF = VEXH x KI
Acceptance criteria: see DATASHEET (3.7, frontal barrier velocity).
3. Check the match with the data released by the manufacturer on the test report.
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8.9.2 DOWNFLOW air (LAF):

Method: air velocity measurement in the working space.
Reference standard: AS 1807.1 (A1); manufacturer’s recommendations.
Instruments: hot-wire anemometer
NOTE 1: typical accuracy: ±3% of reading or ±0,015 m/s, whichever is greater).
DOWNFLOW air velocity is the mean velocity of the laminar air flow (LAF) in the working area
which protects the product on the work surfaces (VLAF).
Check the conditions indicated in 8.9.

Perform this measurement using a hot
wire anemometer (see characteristics
in 1) on a grid of a virtual
horizontal plane, 150 mm below the
downflow filter (see Pic. A18),
according to AS 1807.1 (+A1).
The position of the measurements
must be divided into columns and
rows, both equally spaced.
Follow Tab. 11 to determine the
samples to carry out.
The external points of the grid
coincide with the perimeter of the
area in which the measurements are
made and which will delimit the SAFE
WORKING AREA (see 5.1.6.2).
The anemometer must be supported
by a appropriate support so that it can
be left in place without the operator's
arm interfering with the
measurement.
Pic. A18
Comply with the position of the anemometer in the various readings by following the arrows
shown in Pic. A18.
Tab. 11 – LAF measurement grid

CABINET SIZE ROWS COLUMNS
0.9 3 4
1.2 3 5
1.5 3 6
1.8 3 7
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1. Calculate the average of measurements taken on the DOWNFLOW air (VLAF).

Acceptance criteria: see 3.7.
2. Calculate the percentage deviation between the MINIMUM (VMIN) and MAXIMUM (VMAX)
values with respect to the MEDIUM (VLAF) value.
Acceptance criteria: see DATASHEET (3.7, laminar downflow velocity).
3. Check the match with the data released by the manufacturer on the test report.
8.9.3 CONTAINMENT AT THE FRONT APERTURE:

Method: detection at the front aperture of the aerosol particles generated inside the work
zone.
Reference standard: AS 1807.22.
Instruments: cold aerosol generator and photometer.
Acceptance criteria:
 the penetration of the test aerosol shall, on average, not exceed 0.01% more than
once every 10 s during the total period of test for each test position.
8.9.4 WORK ZONE INTEGRITY:

Method: detection inside the work zone of the aerosol particles generated outside the work
aperture.
Reference standard: AS 1807.5.
Instruments: cold aerosol generator and photometer.
 the test aerosol generated and released outside the work aperture shall not be
detected inside the work zone.
8.9.5 Smoke pattern test:

Method: visualization of airflow patterns with smoke generator.
Standard accordance: EN 12469, Tab. 5; NSF49, clause 6.11; manufacturer’s recommendations.
Smoke patterns shall be determined with the cabinet operating in MODE 1.
 Airflow within the work area of the cabinet shall be downward, with no dead spots,
reflux, or escape from the cabinet.
 Airflow along the entire perimeter of the work access opening shall be inward, with no
reflux out of the cabinet or smoke penetration over or onto the work surface.
 Airflow within the work area of the cabinet shall be downward (no reflux), with no
escape to the outside of the cabinet at the sides and top of the window.
8.9.6 Working area air cleanliness test

Method: air sampling of the working space.
Reference standard: EN 14644-1.
Instruments: particle light scattering counter.
Acceptance criteria: ISO 5 class or better.
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8.9.7 Check of the alarm thresholds:
1. Enter MANUAL mode (see 8.9.8).
NOTE 1: during manual operation mode the fluorescent lamps are lit.
2. Place the vane anemometer at a point corresponding to the calculated average
EXHAUST value (VEXH).
3. Place the hot wire anemometer at a point corresponding to the maximum value found
on the LAF reading grid.
4. Set the percentage of power to have the parameter airflows values measured.
5. Increase the power (%) until the alarm is shown (see Pic. A21).
6. Read the LAF value indicated by the hot wire anemometer (see 2).
The increase of LAF vs the mean value should not exceed 20%.
7. Place the hot wire anemometer at a point corresponding to the minimum value found
on the LAF reading grid.
8. Decrease the power (%) until the alarm is shown (see Pic. A21).
9. Read the INFLOW value indicated by the vane anemometer and multiply it by the KI
coefficient (see Tab. 10).
The decrease of the average value of INFLOW must not exceed 20% and must not be
less than 0.65 m/s.
10. Read the LAF value indicated by the hot wire anemometer.
The decrease of LAF vs the mean value should not exceed 20%.
NOTE 2: the value shown on the display always represents an average value, therefore
when checking the maximum and minimum LAF values, this cannot coincide with what is
indicated by the anemometer.
Normally the gap is equal to the difference between the average value and the value being
checked. Tab. 12 shows how to interpret the alarm value shown on the display.
Tab. 12 – Difference between actual LAF alarm value and that indicated on display
THEORICAL ALARM MINIMUM AND MAXIMUM DISPLAYED ALARM
THRESHOLDS VALUE OF THE LAF GRID VALUE
LAF LAF +20% LAF max A+B-C (MAX)

(average) LAF -20% LAF min A+B-C (MIN)
A B C
The results should match the values indicated in the factory test report (Quality Pass).
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8.9.8 Manual control of the fan:
NOTE: manual control is useful for checking the alarm activation at the set thresholds and
for checking ventilation parameters of the cabinet.
Changes made on this screen are not saved and do not change the calibration of the cabinet.
1. Switch on the cabinet in MODE 2 (see PART 1, 5.2.2).
2. Press the key MODE for 10 seconds.

The display visualizes WARM UP TIME (Pic. A19) and the
power percentage of the fan is automatically set at the set
point value.
At the end of the WARM UP TIME the display will show the
message MANUAL (Pic. A20) Pic. A19
3. To increase or decrease the power of the fan, press the
related keys .
The alarm status will be displayed as following messages:
LAMINAR FLOW,
LOW BARRIER and
Pic. A20
WINDOW POSITION
(see position XXX XXXXXXX in Pic. A21).
Pic. A21
Press the key ENTER (SOCKET) to return to MODE 2.
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9. BIOLOGICAL DECONTAMINATION
The decontamination is a chemical and physical process for destroying all the kind of microorganism.
The decontamination of a BSC is MANDATORY before any service or maintenance operation, replacement
of the filters or any other operations that require access to parts potentially contaminated of the cabinet
and that could present a biological risk against the maintenance operator and external environment,
before moving or disposal of the cabinet and after any emergency.
The choice of the cabinet decontamination method must fall in the risk assessment of the process and of
the activities carried out in the laboratory and must therefore consider the pathogens that are used.
Formaldehyde, which has always been used as an effective contamination agent and proposed in the
guidelines set out in Annex J of the European Standard EN 12469:2000, has been recognized as a
carcinogen and at the issue date of this revision of the manual, several alternatives for decontamination
are under investigation.
An alternative decontamination procedure will be described below, which uses hydrogen peroxide (H2O2)
as decontamination agent.
The procedure has been validated through the use of chemical and biological indicators and it has been
demonstrated the ability to sterilize samples with a concentration of 106 spores of Bacillus
stearothermophilus.
WARNING! See ASSESSMENT OF RISKS, Part 1, warning 1.15
You can use another method of decontamination at the discretion of the laboratory manager.
For this eventuality a function of the cabinet is available (see 9.2.4), which could facilitate the procedure.
In any case it is important that the decontamination procedure is considered into the risk assessment of
the process and activities performed in the laboratory and that a validation of the procedure is performed
using the right chemical and biological indicators (BI) that have a behaviour similar to the pathogenic
agents that are used in the BSC; in particular the BI's should be chosen in order to have a 6-log reduction
and should be positioned in the places where it is more difficult the diffusion of the decontamination agent
(for example in corners of working area, in air diffusion plenum, after exhaust filter).
This procedure must be validated by a method demonstrating compliance with the following requirements:
1) Decontamination effectiveness through the use of chemical and biological indicators correct and
positioned at critical points.
2) Safety of the operator and of the surrounding environment which should therefore not be
contaminated with dangerous substances.
3) Compatibility of the used agent with the mechanical and electrical components of the cabinet.
For the procedure with H2O2, BioAir has considered all the aspects listed above in the definition of the
following decontamination cycle.
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9.1 DECONTAMINATION BY MEANS OF H2O2

The use of hydrogen peroxide (H2O2), as an active ingredient in decontamination, was decided after the
positive result of the validation tests carried out at BioAir to stop the use of formaldehyde and its risks due
to the toxicity of the product.
Hydrogen peroxide is considered safe for the environment as it rapidly degrades into water and oxygen,
which are innocuous products.
The product is supplied as a solution in plastic bottle and it consists of:

 12% hydrogen peroxide (H2O2);
 10 ppm of silver sulphate (Ag2SO4).
WARNING! See ASSESSMENT OF RISKS, Part 1, warning 1.15
9.1.1 OPERATING PRINCIPLE
The solution of hydrogen peroxide and silver is nebulised into particles smaller than 5 μm. In
technical jargon the micronebulization (dry mist), that is the process through which a liquid is
reduced into tiny particles.
The emitted particles are airborne and, remaining suspended, reach all free surfaces.
The cabinet is wrapped in a transparent plastic cover and sealed in order to guarantee the
effectiveness of the process and not to disperse the particles generated by the nebulisation of
H2O2 into the environment.
9.1.2 REQUIRED EQUIPMENT
9.1.2.1 MICRONEBULIZER
Brand: AMIL CARE ITALIA

Model: MEDIBIOS Basic (Pic. A22):
Power: 1000 W
Weight: 9.2 kg
Hourly consumption: 1500 ml/h
Particle size: from < 1 to 5 µm
Remote control (1m)
2 pivoting emission nozzles
 Before using, refer to the product

instruction manual and operating
instructions in this manual.
Pic. A22
9.1.2.2 CONTAINMENT BAG
Plastic bag (Pic. A23) with dimensions

suitable for the size of the cabinet.
9.1.2.3 RICIRCULATION TUBE
Spiralled flexible hose (Pic. A24).
D= 250 mm
L= 2000 mm
Pic. A23 Pic. A24
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9.1.2.4 DECONTAMINATING SOLUTION
Brand: AMIL CARE ITALIA

Product: EVOLYSE STRONG (Pic. A25)
Based on:
 Hydrogen peroxide [12%];
 Silver sulfate [10 ppm].
It is supplied in a plastic bottle with a specific delivery nozzle for the
MEDIBIOS Basic micronebulizer.
Quantity per bottle: 1000 ml
Ready to use. Pic. A25
Toxic by inhalation, in contact with skin and if swallowed.
Hazard pictograms:
Hydrogen peroxide:
(TLV = 1 ppm)
NOTE: check the TLV or

TWA allowed by the legislation Oral Acute Tox. 4
of your Country. Ox. Liq. 1 Skin Corr. 1A
Inhal. Acute Tox. 4
Silver sulfate:
(maximum allowed limit: 0.01
mg/m3)
NOTE: check the value

allowed by the legislation of Aquatic Acute 1
Eye Dam. 1
your Country. Aquatic Chronic 1
 Before using, refer to the product safety data sheet.
9.1.2.5 PROCESS CHEMICAL INDICATORS
Brand: STERIS
Product: VAPORIZED VH202 Process Indicator
Class: 1
Conformity: ISO 11140-1:2014
Single use
Product subject to expiry Pic. A26
Position the indicators (Pic. A26) in the points
shown in Pic. A27
 Before using, refer to the product safety data sheet.
9.1.2.6 VARIE
 Special sealing tape
 Trust of “ATTENTION DECONTAMINATION IN PROGRESS” (Italian/English)
 Portable instrument for H2O2 detection with probe
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9.2 DECONTAMINATION PROCEDURE
WARNING!
Check that the environment in which this decontamination is carried out has a minimum
volume such that when the cabinet is opened, at the cycle end, the concentration of nebulised
H2O2 inside can be diluted in the environment at the allowed levels (see also 9.1.2.4 and the
related ).
To calculate the necessary volume, consult the table Tab. 13.
Tab. 13
TLV ADMITTED INSIDE CABINET AT CYCLE END [ppm] 5
TLV ADMITTED IN THE ENVIRONMENT [ppm]
1 0.7 0.5 0.3
(See the legislation of your Country)
MINIMUM REQUIRED ROOM VOLUME [m3] 20 30 40 70
 Use suitable PPE such as:
fitting safety goggles protective gloves that provides clothing that provides
comprehensive protection comprehensive protection to
the skin
and all the necessary protections when working with corrosive or dangerous substances.
 Use a portable instrument for detecting H2O2 during whole decontamination procedure to
check its concentration.
9.2.1 PREPARATION OF THE CABINET
1. Run the BSC in MODE 2 (see 5.2.2) and lift

the front window to the maximum open
position (see Pic. A27).
2. Position the process chemical indicators
(see 9.1.2.5) in the following points of Pic.
A27:
 lower surface of the LAF filter (not
visible in Pic. A27);
 item 1: outer surface of the exhaust
filter;
 item 2: chamber front wall;
 item 3: work surface;
 item 4: bottom of the working
chamber under the shelves;
 item 5: inner surface of the front
window; and
 item 6: inner surface of the side
windows.
3. Insert the nebulizer in the cabinet (Pic. A27
- item 7) placing it on the right side with
the regulators directed to the left.
Pic. A27
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4. Arrange the nebulizer remote

control and power cables so that
they come out from the front
access of the cabinet (Pic. A28 -
item 5).
5. Insert the bottle of the
decontaminant solution into the
device of the nebulizer (Pic. A22 –
item 1).
(Check that there is sufficient
solution, at least 100 ml).
6. Set the nebulizer main switch to
ON.
7. Lower the front window until it
reaches the working position.
8. Externally apply the 250 mm
diameter flexible hose (see
9.1.2.3) between the exhaust
filter and the front opening (Pic.
A28 - item 1) fixing it with
adhesive tape.
9. Turn off the BSC and leave the
switch key inserted.
10. Unplug the power cord (see

5.2.3.2). Pic. A28
11. Coat the cabinet with the plastic bag (Pic. A28 - item 2) supplied in the decontamination kit
(see 9.1.2.2) by trimming it in such a way as to eliminate the excess parts
(This operation is used to avoid the formation of pockets in which decontaminant residues
may remain in high concentrations).
12. Make the necessary holes to make the following cables come out:
 exit hole of the cabinet power cord (Pic. A28 - item 3); and
 exit holes of the control and supply cables of the nebulizer (Pic. A28 - item 4).
13. Carefully seal all the engraved parts for the arrangement of the bag and the cable passage
holes with adhesive tape.
14. Carefully seal the junction of the plastic bag with the cabinet along the entire lower perimeter
with adhesive tape (Pic. A28- item 6).
15. Check carefully that there are no damages (even small scratches) to the containment bag
and in case seal them with adhesive tape.
16. Apply the "CAUTION DECONTAMINATION IN PROGRESS" warning on the containment bag
and on the outside of the access door to the room.
17. Be sure that there is no risk that an HVAC plant will recirculate air into an adjacent room with
presence of personnel.
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9.2.2 ACTIVATION OF THE DECONTAMINATION CYCLE
1. Connect the nebulizer and the cabinet to the

mains.
NOTE 1: do not use the socket inside

the cabinet, as it is not adequate for the
power of the nebulizer.
On the display of nebulizer will appear the
screen of Pic. A29- item 6.
2. Start the cabinet in MODE 1 (see 5.2.1) and
wait for the flows to stabilize.
3. Switch on the nebuliser by pressing the
STAND BY button (Pic. A29- item 1).
The screen of Pic. 41 will appear on the
display of the cabinet, once the WARM UP is
finished, while the IDLE-ON screen will
appear on the display of the nebulizer, with
a program as in Pic. A30 waiting to be Pic. A29
modified or started.
4. To change the program according to the
values in Tab. 14 press the PROGRAM MODE
button on the nebulizer (Pic. A29 - item 4).
The value that can be changed is the one
that flashes (Pic. A30 - item 2): use the
DOWN and UP keys to change (Pic. A29 -
items 2 and 3).
Pic. A30
NOTE 2: the product of the values in Pic. A30 - items 1 and 2 must correspond to the
quantity of decontaminant indicated in Tab. 14.
For easiness it is advisable to leave the value of item 1 set to 1 ml / m3.
Tab. 14 – Doses and times

CABINET
NEBULIZ
MODE 1
CYCLE DESCRIPTION DOSES AND TIMES
0.9 1.2 1.5 1.8
Q.ty H2O2 (ml) 25 30 40 50

PHASE 1 ON ON
ESTIMATED TIME (mm:ss) 01:00 01:12 01:36 02:00
CONTACT 1 OFF ON REQUIRED TIME (min) 60
Q.ty H2O2 (ml) 25 30 40 50

PHASE 2 ON ON
ESTIMATED TIME (mm:ss) 01:00 01:12 01:36 02:00
CONTACT 2 OFF ON REQUIRED TIME (min) 15

REDUCTION OF
OFF OFF REQUIRED TIME (h) 12
H2 O2
CHECK OF H2O2 CONCENTRATION INSIDE CABINET (< 5 ppm)
END OF CYCLE OFF OFF CHECK OF H2O2 CONCENTRATION IN AMBIENT (see 9.1.2.4 and
related note ).
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WARNING!
The doses and times indicated in Tab. 14 for the decontamination cycle with this method
refer only to the volume created by the containment bag.
If for some reason it is necessary to act on larger volumes, the times and the doses
must be recalculated through a new validation of the necessary procedure.
5. Press the PROGRAM MODE key on the external keyboard of the nebulizer again (Pic. A29 -
item 4) to confirm the program.
6. Make sure that there is no personnel in the room affected by this decontamination.
WARNING!
During the whole decontamination phase, equip yourself with adequate portable
instrumentation to check the level of H2O2 in the environment remains within allowed
limits (see 9.1.2.4 and related ).

7. Start the first decontamination cycle by pressing the STAR / STOP button (Pic. A29 - item 5).
The relative LED flashes until the end of the cycle.
A short intermittent acoustic signal is emitted for 15 seconds (countdown) to indicate that
the cycle has been activated.
The countdown allows leaving the environment in which the machine is placed, once pressed
the START / STOP key, before the real cycle begins (product dispensing).
8. Leave the room.
At the end of the countdown the display shows the message in Pic. A31.
The letter R on the side of the countdown serves

to point out that filling of the tank is in progress.
The cycle remains in stand-by until tank filling is
completed.
Pic. A31
NOTE 3: if this filling lasts for longer than 150 seconds (therefore the pump remains
active for longer than 150 seconds) it is assumed the bottle is empty (no functional block is
provided for).
The display will show this condition with the message: ALM BOTTLE.
To reset the signal it is necessary to replace the bottle and then restart a cycle of
decontamination. The bottle release method is described in 9.2.3.1.
Once the decontaminant load has been
completed, the display shows the message in
Pic. A32 with the start of H2O2 dispensing and
the set time decrease.
Once the program minutes/seconds have
elapsed, the START/STOP LED turns off while the Pic. A32
display returns to display the message in Pic.
A30.
9. Leave the cabinet switched on and wait for 60 minutes.
10. Start the second decontamination cycle by pressing the STAR/STOP key.
11. Leave the room.
12. After the second cycle, wait 15 minutes and then switch off the cabinet.
In this condition the dispensing phase of decontamination cycle is ended.
NOTE 4: in case of interruption of mains electrical supply, when power is reinstated the
unit goes back to the state (idle-on or stand-by) that it was in at the time of power failure.
If a decontamination cycle is performed during the interruption, it is aborted.
All the data set remain stored. The decontamination is to be repeated.
13. Leave the cabinet sealed and wait 12 hours to allow the H2O2 concentration to be reduced.
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9.2.3 REMOVAL OF THE DECONTAMINATION SYSTEM
WARNING!
Even if the cabinet is sealed and the recorded level of H2O2 during validation test is lower
than allowed limit, consider always that something could go wrong and so use all the
necessary precautions before to enter into the room.
Before proceeding with this operation after decontamination cycle, use the necessary
precautions: it is recommended the use of PPE (mask, gloves, glasses, etc.) and verify
by means of suitable equipment that the H2O2 concentration in the room be lower than
the allowed value (see 9.1.2.4 and related ).

1. Check that the concentration level of H2O2 in the room is less than the allowed limit (see
9.1.2.4 and related ).

2. Check that the color of the chemical process indicators is set as required by the instructions
(see also Pic. A26).
NOTE 1: if the result is not what is expected, repeat the decontamination.

In case of new failure, contact BioAir technical assistance.
3. Switch off the nebulizer disconnecting it from the mains.
4. Drill a small hole in the plastic bag that seals the cabinet to check by introducing a suitable
probe that the concentration of H2O2 inside is less than 5 ppm.
NOTE 2: in case the concentration is higher, seal the hole made and repeat the check
after 4 hours.
5. Once the concentration level is stabled within the limits, remove the plastic bag, the hose and
the chemical indicators.
In this stage still wear protective devices.
6. Run the cabinet in MODE 1 for at least 15 minutes.
7. Check that the concentration level of H2O2 in the cabinet and in the room is less than the
allowed limit (see 9.1.2.4 and related ).

8. Remove the adhesive tape that seals the front opening and lift the window up to the
maximum opening.
9. Remove the decontaminant bottle following the instructions in 9.2.3.1.
10. Remove the nebulizer.
The cabinet is now ready for the necessary operations (use and service or maintenance).
9.2.3.1 H2O2 bottle extraction

The bottle has to be extracted in the following way:
1. Firmly press the unlock mechanism (see Pic. A22 – item 2).
2. Hold pressed the release mechanism and lift the bottle.
3. Release the unlock mechanism (valves will automatically close).
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9.2.4 Automatic cycle for alternative fumigation
WARNING!
The following instructions explain only how to use the function for a possible alternative
decontamination method that must be validated by the user.
9.2.4.1 PROCEDURE
1. Turn on the cabinet in MODE 2 (see
PART 1, 5.2.2).
2. Close the front window.
3. Press the keys , and at

the same time. Pic. A33 Pic. A34
The display shows the screen to access
the password (Pic. A33).
4. Press the key ENTER (SOCKET).
The display shows the message to enter the password (Pic.
A34).
5. Set the correct password by pressing the keys UP (LIGHT)
and DOWN (UV).
The password for fumigation is 9.
Pic. A35
6. Press the key .
If the password is correct, the display shows FUMIGATION IN
PROGRESS (Pic. A35); the service socked is activated to start
the first phase of the cycle (dispensing of the decontaminant).
NOTE 1: determine the phases and times of

decontamination.
Pic. A36
7. Remove the key of the switch and wait for the time established
for the dispensing phase.
NOTE 2: in case of black out, when the power is reactivated

the display will show the message of Pic. A36.
8. Elapsed this time press the keys e and hold them for Pic. A37
30-40 seconds.
The service socket is turned off and the fan starts. Display will
show the message FUMIGATION RECIRCULATION (Pic. A37).
9. Release the keys.
The fan stops. Display shows FUMIGATION IN PROGRESS (Pic.
A35).
10. Leave the cabinet in this status for the time established for the Pic. A38
contact phase.
11. Past this time restart the cabinet.
PURGE mode is activated: the fan starts (FUMIGATION PURGE
Pic. A38) at reduced velocity and the front window will be
partially opened (few centimetres).
12. Remove the switch key and leave the cabinet running in
FUMIGATION PURGE for the time established for the purge
Pic. A39
phase.
In case of blackout, when the power is reactivated the display
will show the message of Pic. A39.
13. After PURGE phase turn off the cabinet and remove the used
equipment.
Pic. A40
NOTE 3: the function is set with predefined values of the
times and characteristics of the cycle phases (see Pic. A40).
To modify these parameters, request instructions from BioAir.
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10. SERVICE AND MAINTENANCE
WARNING!
Biological hazard
The internal parts of the BSC are to be considered contaminated.
Mechanical and electrical hazard

The electrical and electromechanical components are potentially active until the cabinet
power cable is disconnected.
 Decontaminate the BSC (see 9.1) before opening the access panels to the filters and fan
motor.
 Unplug the power cord from the mains outlet before accessing the components.
 Do not perform any operations before obtaining the necessary instructions from BioAir.
WARNING! UV-C radiations hazard

During a technical service due to a failure to the consent UV system power-on or in case of
breaking of the front window, the taken protection on the cabinet can not be guaranteed.
 Make sure there are no unprotected people around the cabinet.

 Remove the UV-C lamp or make it inactive by intervening in the control panel.
 If this is not possible, use a screen (or wear appropriate clothing, gloves and
goggles/masks) to protect the skin and eyes from direct or reflected radiation from UV-C
rays.
MANIFESTED AND FOR DAMAGE TO PERSONS AND/OR THINGS DUE TO NON-AUTHORISED
INTERVENTIONS AND/OR WHICH ARE NOT COMPLIANT TO THE OPERATING INSTRUCTIONS CONTAINED
IN THIS MANUAL.
10.1.1 ROUTINE CHECKS

Routine maintenance tests should be carried out at regular intervals or as determined by regulatory
authorities. It is recommended to carry out the checks with the frequency indicated in Tab. 15.
Tab. 15
DESCRIPTION HOW OFTEN DO
(months)
A Filters integrity 6
DOWNFLOW and INFLOW air velocity 6
Containment at the front aperture 6
Displaying ventilation parameters 6
Alarms 6
Smoke pattern test 6
B Defects, cracks or other damage of internal and external surfaces 12
Extraction duct system (if present) 12
Anti-blow back valve (if present) 12
Protective conductor efficiency [EN 61010-1, annex F] 24
The procedures for verifying performance (A) are indicated in 8.7.
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10.1.2 FILTERS REPLACEMENT

The clogging of the filters depends on various factors, including the degree of cleanliness of the air
in the room in which they work.
The self-regulating air flow system allows compensating for the clogging of the filters and keeping
the air velocity values within the safety limits.
It is however advisable to replace the filters no later than the limit of 6000 working hours or 5
years regardless of the number of working hours.
The replacement of the filters represents an intervention on critical parts of the
cabinet and involves the acquisition of an adequate training level.
Such an intervention can compromise the operation of the cabinet and the
biological safety of operators, environment and product
The replacement is possible only by BioAir technicians or by technicians
authorized and trained by BioAir.
Contact BioAir to request the training and technical information necessary for such an intervention.
10.1.2.1 DISPOSAL OF FILTERS
WARNING!
 HEPA filters should be decontaminated in situ prior to disposal.
 The requirement for, and the method of, decontamination must be by risk
assessment (see also par. 9).
Once removed they must be sealed and identified as a HAZARDOUS WASTE.

Therefore they must be treated by companies qualified and authorised for the disposal of such
waste.
 Seal each individual filter in a polythene bag.
 Use suitable containers for temporary storage.
 Identify the container with appropriate label showing the type of waste.
 Start the correct disposal procedures according to the local regulations in force.
10.1.3 REPLACEMENT OF VARIOUS PARTS

Replacement of electrical, electronic, mechanical and electromechanical parts
represents an intervention on critical parts of the cabinet and involves the
acquisition of an adequate training level.
Such an intervention can compromise the operation of the cabinet and the
biological safety of operators, environment and product
The replacement is possible only by BioAir technicians or by technicians
authorized and trained by BioAir.
Main spare and consumable parts are listed in the table in 10.3.
Contact BioAir to request the training and technical information necessary for such an intervention.
10.1.4 CALIBRATION AND PC BOARD SETTING

Access to these parameters involves the acquisition of an adequate training
level.
Possible changes can compromise the operation of the cabinet and the biological
safety of operators, environment and product
Modifications are possible only by BioAir technicians or by technicians authorized
and trained by BioAir.
Contact BioAir to request the training and technical information necessary.
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10.2 TROUBLE SHOOTING

These operations shall be carried out under safe conditions.
WARNING!
Biological hazard.
Some components are located in contaminated area.
Electric shock hazard.
Some components remain hazardous live despite the cabinet being switched off via the key
switch.
 Contact BioAir for the training necessary for interventions inside the cabinet.
 In the event of an emergency being necessary, carry out the following actions:
 decontaminate the cabinet before accessing internal parts and components;
 disconnect the cabinet from the mains by means of the plug on the power
cable.
Tab. 16 – Trouble shooting

Trouble Suggested check
1. BSC is plugged to mains and  the mains voltage at the mains supply socket;
switched ON.  fuses F10 and F20 at appliance mains inlet (see 2.3.3).
The LED and display do not  Refer to the technical documentation for authorized service.
light up.
Ventilation does not start.
2. The BSC is ON.  the cabinet is running in MODE 1.
Ventilation does not work.  Refer to the technical documentation for authorized service.
3. The BSC is ON.  the UV function is off;
Lighting does not work.  the LIGHT key LED is on;
 the lighting lamps (see 7.4.2).
 Refer to the technical documentation for authorized service.
4. BSC is ON.
Front window does not move.
5. BSC is ON.  the SOCKET key LED is on;
Service socket is not  Refer to the technical documentation for authorized service.
energised.
6. BSC is ON.  the cabinet is running in MODE 2;
UV-C lamp does not work.  that the lighting is OFF;
 the UV key LED is on;
 the front window is closed completely;
 the UV lamp (see 7.4.1).
7. BSC is on.  the cabinet runs in MODE 1;
The solenoid valve of the  no alarms are active;
flammable gas line does not  the GAS key LED is on.
operate.
8. BSC is on.  follow instruction in 5.1.3.1.
The front screen stops while  Refer to the technical documentation for authorized service.
lowering.
NOTE: if the fault is not solved contact BioAir.
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10.3 SPARE PARTS LIST
10.3.1 Common replacements

Quantity
Code Description
0.9 1.2 1.5 1.8
80.007.116 Adjustable foot 4 4 4 4
80.009.047 Fluorescent lamp 30 W -- 2 -- --

80.009.048 Fluorescent lamp 58 W -- -- -- 2
80.009.053 UV-C germicidal lamp T8 15W 1 -- -- --
80.009.054 UV-C germicidal lamp T8 30W -- 1 1 --
80.009.084 Main fuse F10A H, 250V 2 2 2 2
80.009.090 Fluorescent lamp 36 W -- -- 2 --
80.009.160 UV-C germicidal lamp T10 40W -- -- -- 1
80.009.248 Fluorescent lamp 25 W 2 -- -- --
80.009.392 Mains supply cord – AU plug 1 1 1 1
80.009.391 Mains supply cord (alternative - DE/FR plug) 1 1 1 1

80.009.394 Mains supply cord (alternative - CH plug) 1 1 1 1
80.009.404 Mains supply cord (alternative - UK plug) 1 1 1 1
80.014.049 Shelf extraction hook 1 1 1 1
80.017.199 Soft touch keypad 1 1 1 1
10.3.2 Critical replacements

Quantity
Code Description
0.9 1.2 1.5 1.8
80.003.002 Solenoid valve 24Vac (OPTIONAL) 1 1 1 1
80.003.076 Gas spring 250N 250 mm 2 2 -- --
80.003.087 Gas spring 350N 250 mm -- -- 2 2
80.004.051 Safety glass -Front window SM 1.2 -- 1 -- --

80.004.052 Safety glass -Front window SM 1.5 -- 1 -- --
80.004.057 Safety glass -Front window SM 0.9 1 -- 1 --
80.004.062 Safety glass -Front window SM 1.8 -- -- -- 1
80.008.122 Motorblower 1 1 1 1
80.009.403 Service socket DE (alternative) 2 2 2 2
80.009.440 Service socket FR (alternative) 2 2 2 2
80.009.441 Window sash motor 20 Nm 1 1 1 --

80.009.442 Window sash motor 30 Nm -- -- -- 1
80.009.545 Service socket AU 2 2 2 2

80.009.546 Service socket UK (alternative) 2 2 2 2
80.009.592 Service socket CH (alternative) 2 2 2 2
80.010.004 HEPA filter -LAF 610x1220x68mm -- 1 -- --

80.010.022 HEPA filter -LAF 610x1829x68mm -- -- -- 1
80.010.025 HEPA filter -LAF 610x1525x68mm -- -- 1 --
80.010.028 HEPA filter -LAF 610x915x68mm 1 -- -- --
80.010.030 HEPA filter -Exhaust 457x610x115mm -- 1 -- --
80.010.032 HEPA filter -Exhaust 457x915x115mm -- -- -- 1
80.010.042 HEPA filter -Exhaust 457x762x115mm -- -- 1 --
80.010.132 HEPA filter -Exhaust 457x457x115mm 1 -- -- --
80.011.097 Control and power board 1 1 1 1
80.013.042 Front window belt 2 2 2 2
NOTE: see 10.1.3.
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10.3.3 Full list
The complete list of spare parts will be made available to service personnel
who have received adequate training from BioAir.
11. WIRING DIAGRAM
The wiring diagram will be provided to the service staff adequately trained by
BioAir.
Contact BioAir to request the necessary training and technical documentation.
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Operation Manual SM ECO ABC - Bioair

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Australian Biosafety Cabinet

Class II Biological Safety Cabinets

SafeMate Eco+ ABC

+39 0382 6672.1

5.1.4 UV-C germicidal lamp 41

6. GENERAL RULES – GUIDELINES 56

8.4 DISPOSAL OF THE PACKAGING 71

10. SERVICE AND MAINTENANCE 91

ALL RIGHTS ARE RESERVED ACCORDING TO THE INTERNATIONAL COPYRIGHT CONVENTIONS,

WHAT IS SUPPLIED INSIDE

ON THE TOP INSIDE WORK ZONE

Intake front grille.

Power cord Work surface.

ON THE SIDE OF CABINET

BIOHAZARD Inert gas tap

ELECTRIC SCHOCK HAZARD WARNING

BIOLOGICAL HAZARD WARNING

PINCH HAZARD WARNING

UV RADIATIONS HAZARD WARNING

DO NOT USE products containing chlorine

DO NOT USE RADIOACTIVE MATERIAL

DO NOT USE CYTOTOXIC / CHEMICAL MATERIAL

DO NOT USE IN RESIDENTIAL AREAS

DO NOT USE IN EXPLOSIVE RISK AREAS

GENERAL SIGN OF MANDATORY PROVISIONS TO BE IMPLEMENTED

SEE SPECIFIC DOCMENTATION

MAIN GROUND TERMINAL BLOCK

Separated collection for electric and electronic equipment.

NOT FOR USER, Service information only

OPERATION THAT REQUIRES TWO PEOPLE

OPERATIONS ALLOWED ONLY TO PERSONNEL TRAINED AND AUTHORIZED BY BioAir

WARRANTY AND LIABILITY

 IMPROPER USE of the cabinet

DEFINITION OF IMPROPER USE

NOT ALLOWED USE

Tab. 1 – List of handling and products

 Handling of non-pathogenic biological sterile materials ABSENT

 Preparation of non-toxic pharmaceutical substances ABSENT

 Plant cell cultures ABSENT

 Treatment of thin films ABSENT

 Transplantation on small animals ABSENT

 Assembly of electronic components ABSENT

 Handling of pathogens (see in INTENDED USE) BIOLOGICAL

 Handling of products potentially contaminated by

 Handling of chemicals CHEMICAL

 Preparation of cytotoxic drugs CHEMICAL/TOXIC

 Generic use of radionuclide RADIOACTIVE

allowed use not allowed use

HEREWITH DECLARES THAT THE FOLLOWING PRODUCTS:

DESCRIPTION: CLASS II BIOLOGICAL SAFETY CABINETS

COMPLY WITH THE FOLLOWING STANDARDS:

 IEC 61010-1:2010 Safety requirements for electrical equipment for measurement,

 AS 2252.2—2009 Controlled environments. Part 2: Biological safety cabinets Class II

OTHER ADDITIONAL HARMONISED EUROPEAN STANDARDS PARTIALLY APPLIED:

 EN 12469:2000 Biotechnology - Performance criteria for microbiological safety

APPROVALS AND CERTIFICATIONS

WITHIN ITALY ABROAD

For any additional information, please contact the manufacturer:

NOTE 2: no residual risk of this equipment belongs to Category 3.

NOTE 3: read the following warnings before operating this isolator.

1.1 General hazard. Risk category: 1

1.2 Misuse hazard. Risk category: 1

 Misuse (see paragraph DEFINITION OF IMPROPER USE) compromises the