Professional Documents
Culture Documents
2031219
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03-10/03/11
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03-10/03/11
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2031219
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03-10/03/11
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03-10/03/11
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03-10/03/11
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2031219
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03-10/03/11
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2031219
03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
100 ?
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2031219
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03-10/03/11
" 805
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2031219
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03-10/03/11
) (
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B
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2031219
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03-10/03/11
" B . B
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2031219
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03-10/03/11
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22
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2031219
03-10/03/11
, .
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23
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2031219
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03-10/03/11
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2031219
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03-10/03/11
. IgG ,
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2031219
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03-10/03/11
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2031219
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03-10/03/11
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2031219
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03-10/03/11
56C- ,
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27
28
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2031219
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03-10/03/11
IgA ) , ,
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29
2031219
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03-10/03/11
) =(Isotypes ,
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30
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2031219
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03-10/03/11
)=( .
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IgG-1, IgG-2, IgG-3 .IgG-4-
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IgG-1 .IgGs-
IgG-3- t1/2- .
IgG-4 , IgG-3
.
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2031219
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03-10/03/11
IgG-1 IgG-3- ,
Fc- "
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constant IgG-1 ) , (,
class switching- constant- IgG-3'.
" , ) ,
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32
2031219
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03-10/03/11
= , Fc- ,
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33
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2031219
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03-10/03/11
24-48- .
.IgM
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34
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2031219
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03-10/03/11
.
)= (
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35
2031219
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03-10/03/11
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36
2031219
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03-10/03/11
(1) : )
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2031219
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03-10/03/11
, ,
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2031219
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03-10/03/11
FACS
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(FACS (Fluoresence Activated Cell Sorter-
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B'( .
38
39
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'
2031219
'
03-10/03/11
. ,
) ( .
FACS
.
) (
) (.
T-killer
.
=) Ex-Vivo (.
39
40
2031219
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03-10/03/11
5
: - , , , NK
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)
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41
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2031219
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03-10/03/11
B -
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B , T
.
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T ConA.PHA-
) B ( ) LPS ,
,B B
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PWM B ,T .
)(Complement
.
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41
42
'
2031219
'
03-10/03/11
.
- )
(.
Fc .
IgM . 5
.Fc Fc -
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-
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C1 C4 C1. C4-
C2 C4-
C1 C2 - C3 C2+C4 - C3
C3/C5
, , .
C2+C4+C3 C5 C5
C6 C7 - C5+C6+C7 C8
C9
.
":
42
43
2031219
'
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03-10/03/11
http://www.youtube.com/watch?v=WuhbRKFpqZc
Complement Receptor
C3 )=(.
) Fc- Fc-
(.
C5+C6+C7 )= (.
C3 .
, . -
.
, .
-
) (.
+ , .
, .
43
44
:
:
2031219
'
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03-10/03/11
)= :(Immature
).(White Pulpe-
Mature
.
B T
) (
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- .
) , IgA- ,
( , ,
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.
44
45
2031219
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03-10/03/11
T T-killer- "
, T .(T-killer (=Natural Killers=NK
.T-killer .
.
,
) .(NK
NK
: "
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NK Fc (
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45
46
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:
2031219
'
'
03-10/03/11
)
(.
:NK
NK- : , Granzymes-
.
46
47
:
:
'
2031219
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03-10/03/11
47
48
2031219
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03-10/03/11
6
: ) ,(MHC= Major Histocompatability Complex
MHC- ,
) T (.
, T .
: , ,
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MHC
MHC , MHC .
MHC - ,
.
MHC =) Crossing-Over ,
( Gene Conversion , .MHC-
:MHC
-MHC Class I .
) , , MHC
( , MHC-1 .
T ,
, ) ,
( T , )
(.
48
49
'
'
2031219
03-10/03/11
-MHC Class II ,
, .
, ,
) ( .
.MHC-2
"
- .
MHC Class I
MHC-1- ) - (,
A, B :) C- (.
" - ) ( ,
) , (
MHC-1.
- 6 MHC-1- )
(.
- ,MHC-1 )=(.
49
50
:
:
2031219
'
'
03-10/03/11
MHC-1- :
.
.
.
,
) (.
- , MHC- .
, .
- .
MHC--
1 T -
T ,
.
- ,
.
MHC ) 6(
MHC - .
50
51
'
2031219
'
03-10/03/11
,
, .
) MHC-1
( , ) B
MHC- , (.
:MHC
-.
" .
ER. -
, ) ( -
. MHC- .
MHC .
MHC ER- MHC
, ) ( -
ER " -.
51
52
:
:
'
2031219
'
03-10/03/11
. ,
MHC-" -
.
- T ,
. - T .MHC-1 Restricted
- ,
.
- MHC- ,
, )
(.
MHC HLA
,T HLA- HLA
T-killer ) A1 .(A1 , HLA ) A2)
.
MHC Class II
52
53
'
2031219
'
03-10/03/11
. , .
MHC-2- A, B ,C- .DP, DQ, DR
: .
3 ," 6 ,
,MHC-1 .
:MHC-2-
MHC-2 ,ER- ) -
.(MHC-1 ER- ) ( .
)( "
.MHC-2
- MHC-2-
) (
MHC-2- - .T
53
54
'
2031219
'
03-10/03/11
- :
http://www.youtube.com/watch?v=_8JMVq7HF2Y
.
, )=.(CD
.
- . - B
.
) SIgM .(IgM , B - SIgM
, .
" SIgM
.
SIgM B
SIgM- B .B
) EBV- ( .B EBV
.B
,CD16 , ,Fc- ) (
.
54
55
'
2031219
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03-10/03/11
B . B
" .MHC-2
:
-(T (T Independent ,B ,
.T
" ,
) B ,
B , (T
55
56
:
:
'
2031219
'
03-10/03/11
, .T Dependent-
B .
?
T-killer
T-killer , - )
(.
T-killer ) , " ,(
, ?
.
: ) "(
. ,
.
56
57
'
2031219
'
03-10/03/11
7+8
: T
T ) ,(B
.
) (.
, MHC
.
, " ) MHC
, .(B
2 : .
,
" .
, " TCR
) ,"
(.
57
58
'
2031219
'
03-10/03/11
,CD-3 .T
CD-3 :
TCR ER- ,
.
-.
) (B T )=( ,
, ) (.
- T MHC - .
T . " MHC . .
- TCR ,T
:MHC
MHC - ,MHC
TCR- -.
58
59
:
:
'
2031219
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03-10/03/11
CD-4CD-8-
)
MHC- , (.
CD-4 , " T-helper
CD-4+ (TH1 (TH2- .MHC-2
T-helper )-
,B (.
CD-8 " T-killer ,(CD-8+ (Tc=Tk
.MHC-1 T-killer .
- " TCR
MHC" CD-4 CD-8
T , .
) T-helper T-killer CD-4 (CD-8 MHC- )
MHC-1 (MHC-2 " .MHC-
T , :
MHC.
) (.
59
60
:
:
'
2031219
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03-10/03/11
T
.
, .
=Priming T ,
" .
Priming- T " )
(CoStimulation Clonal Expansion of Naive T
Cells .
T ) MHC-1 , -
" ,MHC- -
? ?( =) Professinal ,B
( , : ) co-Stimular B7+
(CD-28 Professionals .MHC-
60
61
:
:
'
2031219
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03-10/03/11
CD-8
CD-8 :
61
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2031219
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03-10/03/11
) IL-2 - ( CD-8
.
-CD4" CD-4
.CD-8
CD-4 CD-4
.
- Priming- :CD-8-
CD-8 ,IL-2 IL-2
.
CD-8 :
.
CD-8 :
) , (
. )
( .
62
63
'
2031219
'
03-10/03/11
, .
T-killer CD-8+
.
CD-4 CD-8 .
CD-4
-T-helper 1 ,
.T-killer
-T-helper 2
.B
) , (
, , , "
.
" .
, T-helper ,
" .
63
64
:
:
'
2031219
'
03-10/03/11
" MHC-2
. - ) T MHC
TCR) - " T , TCR-.
64
65
:
:
'
2031219
'
03-10/03/11
T , ,
) (
.
65
66
:
:
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2031219
'
03-10/03/11
9+10
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= ,
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) ,( , ."
. , A'.
) (IgG-
.
- )
( . - .
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/ -
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) ( .
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66
67
:
:
2031219
'
'
03-10/03/11
HA : .NA-
"
. HA NA- .
HA "
?
HA ,
HA- .
NA .
o
/ - ) B
( ,S )(
.
DNA- , .S
S (HBSAg (Hepititis B Surface Antigen
)( .B
)= ,(Subunit Vaxin )
( ) (.
, MHC
) 1 (2 S
.
,
, ,
.
o
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67
68
2031219
'
'
03-10/03/11
)
( , ".
, .
, , .
Surface ,
, .
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)
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, ,
.
, , , 10- .
6- -
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-Variolation .
,
. - ; -
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69
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2031219
'
03-10/03/11
19- ' .
,
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100- ,
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69
70
2031219
'
'
03-10/03/11
, .
) ( )(.
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, .
, , .Antobodies
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20-" .
, .
)=
( .
) (
.
) T ,B- ( ,
.
70
71
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2031219
'
03-10/03/11
" .
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) , (.
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.
: ,
.
. T-killer
.MHC-1 , , ,
.
: ,T-helper
.T-killer
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72
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2031219
'
03-10/03/11
70- ,
.
__
) B .(1982
, ,T-helper B
, .
B - ,B
T-helper " ,B
, B
.
__
)(Conjugate
.
) ( .
, " MHC-2 B , T-helper
B .
, , )-
( .
.
) ,( . B
+ " MHC-2 )( .
72
73
'
2031219
'
03-10/03/11
T-helper B
, B , ,
, .
, , 20-
) (.
__
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.
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(.
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- .
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= .
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2031219
'
03-10/03/11
,
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5- .
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75
2031219
'
'
03-10/03/11
, , ' ) (85% )
,(15% ,DNA- " .
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DNA) - .
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) EBV ( )= (.
.
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76
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2031219
'
'
03-10/03/11
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76
77
2031219
'
'
03-10/03/11
, .
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77
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'
2031219
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03-10/03/11
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