Scribd Logo
Upload

Welcome to Scribd!

  • Análisis Crítico - Artículo 2: Modeling Bioprocess Scale-Up Utilizing Regularized Linear and Logistic Regression

    Uploaded by

    Laura Castellanos Suárez
    17 views2 pages
    Análisis artículo 2

    Original Title

    Artículo 2

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    Análisis artículo 2

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    17 views2 pages

    Análisis Crítico - Artículo 2: Modeling Bioprocess Scale-Up Utilizing Regularized Linear and Logistic Regression

    Uploaded by

    Laura Castellanos Suárez
    Análisis artículo 2

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Análisis Crítico - Artículo 2

    Modeling bioprocess scale-up utilizing regularized linear and logistic regression

    TÓPICOS AVANZADOS EN BIOTECNOLOGÍA

    9 de septiembre de 2018
    Autor: Laura Johana Castellanos Suárez
    Análisis Crítico - Artículo 2
    Modeling bioprocess scale-up utilizing regularized linear and logistic regression
    Muhammad Farhan, Antti Larjo, Olli Yli-Harja, Tommi Aho
    IEEE INTERNATIONAL WORKSHOP ON MACHINE LEARNING FOR SIGNAL PROCESSING –
    2013

    The paper proposed a computational scale-up approach applicable to various


    bioprocesses. The method bases on regularized linear and logistic regression. With
    embedded feature selection, it automatically identifies the most influential parameters
    and predicts their values in large scale. In addition, the method predicts the resulting
    large scale yield. As a case study, they examined the production of a cytotoxic compound
    and predicted scale-up from flask and 2L to 30L fermentations and found that, in both
    cases, the product yield predictions were close to experimentally observed yields.

    The scale-up methodology was consisted of four steps: encoding of categorical variables
    to incorporate them into modeling, product yield prediction using regularized linear
    regression, yield correspondence-based data rearrangement and scale-up modeling using
    regularized linear and logistic regression. Experiments were performed in flasks (81
    samples), 2L fermenters (24 samples), and 30L fermenters (12 samples). Each sample
    was composed of around 40 typical bioprocess variables such as strain, broth medium,
    broth adsorbent, fermentation time, temperature, agitation, aeration, pH, etc. After
    dummy coding the categorical variables, the number of variables increased to more than
    70. All the samples were exploited when developing the yield prediction model using
    regularized linear regression. Then 10-fold cross-validation was performed to determine
    the optimal model coefficients based on the minimum prediction error so that the model
    is general enough to predict the product yield for an unseen sample.

    The conclusion was that the scale-up was successfully achieved from flask to 30L
    fermenter, as well as from 2L to 30L fermenter. Instead of these lab-size fermentations,
    Análisis Crítico - Artículo 2 | 9 de septiembre de 2018

    the values of operational parameters in 30L fermenters could be determined based on


    flask experiments.

    It was not clear if the authors used their own software or code, or commercial software to
    develop the models, optimize or even analyze the results. In the same way, I think that in
    order to give more credibility to this new methodology, they should have developed a
    greater number of experiments in the 30 L bioreactor because the experiments were less
    than in the small ones.

    In think the paper content had deep optimization and statistics themes. Therefore, it was
    necessary to make a careful reading of it. Likewise, in my opinion if you want to apply this
    methodology you should study a lot about the methods proposed to get successful
    results. On the other hand, the results were surprising as a consequence of the detailed
    mathematical development. I think it could be a breakthrough in the bioprocesses scale-
    up and in saving costs and time for companies.

    You might also like