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  • Effect On Alkaline Phosphatase: Temperature

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    This document examines the effect of temperature and activators on alkaline phosphatase enzyme activity. It finds that enzyme activity increases with temperature up to an optimal level of 37°C, above which the enzyme denatures. Certain metal ions like Mg2+ and Ca2+ act as activators by binding to the enzyme and increasing its activity, while others like Fe2+ decrease activity. The optimal activator is Mg2+, which serves as a metal cofactor to facilitate the enzyme's catalytic function.

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    Biochemistry Lab

    Original Title

    Effects TempandActivators

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    This document examines the effect of temperature and activators on alkaline phosphatase enzyme activity. It finds that enzyme activity increases with temperature up to an optimal level of 37°C, above which the enzyme denatures. Certain metal ions like Mg2+ and Ca2+ act as activators by binding to the enzyme and increasing its activity, while others like Fe2+ decrease activity. The optimal activator is Mg2+, which serves as a metal cofactor to facilitate the enzyme's catalytic function.

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    © All Rights Reserved
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    6 views10 pages

    Effect On Alkaline Phosphatase: Temperature

    Uploaded by

    t-o
    This document examines the effect of temperature and activators on alkaline phosphatase enzyme activity. It finds that enzyme activity increases with temperature up to an optimal level of 37°C, above which the enzyme denatures. Certain metal ions like Mg2+ and Ca2+ act as activators by binding to the enzyme and increasing its activity, while others like Fe2+ decrease activity. The optimal activator is Mg2+, which serves as a metal cofactor to facilitate the enzyme's catalytic function.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 10

    Effect on Alkaline

    Phosphatase: temperature

    Effect of Temperature
    Temperature O.D. Reading
    at 660 nm
    5
    0.180

    Phosphate
    concentration
    3.80 x10-4

    25

    0.244

    5.3 x10-4

    37

    0.481

    9.6 x10-4

    60

    0.149

    3.2 x10-4

    Effect of Temperature

    Effect of Temperature
    Increase in temperature = increased
    enzyme activity
    A ten centigrade rise in temperature
    = increased enzyme activity by 50100%
    Optimal temperature: 37 degree
    Celsius
    Enzyme activity at its greatest

    Denaturation

    Effect on Alkaline
    Phosphatase: activators

    Effect of Activators
    Activator
    Mg++

    O.D. Reading
    at 660 nm
    0.456

    Phosphate
    concentration
    9.9 x10-4

    Ca++

    0.367

    7.96 x10-4

    Fe++

    0.083

    1.8 x10-4

    Mg++ + TCA

    0.059

    1.28 x10-4

    Effect of Activators

    Effect of Activators
    Activators: molecules that bind to
    enzymes, increase activity
    Metal ion cofactor
    Required for catalysis
    Metalloenzymes: contain metal ions

    Effect of Activators
    Metal ion cofactor
    Bind to substrate to change orientation
    Mediate oxidation-reduction reactions,
    reversible changes
    Electrostatically stabilizing or shielding
    negative charges

    Effect of Activators
    Effect of Mg
    Metal cofactor
    Lewis acid and chelate formation
    Bridge complex formation

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