Diagnosis

Clinical inspection
Viral cultures
Positive within 2-4 days of inoculation
Polymerase chain reaction (PCR) assay
Most accurate and sensitive technique for identifying
herpes virus
Serologic tests
Western blot assay
Tzanck smear
 Lymphogranuloma Venereum
Rule In Rule Out Diagnostics
- Incubation period (3-30 - Usually one lesion - Culture
days) - Unilateral adenopathy - Direct
- Shallow vulvar ulcer - (-) Groove sign immunofluorescence
- Diameter of ulcer (2-10 - Nucleic acid detection
mm) - Chlamydia serology
- Non-tender inguinal (complement fixation
lymph nodes titers >1:64)
- Malaise
- Fever
 Chancroid
Rule In Rule Out Diagnostics
- Incubation period (1-14 - Unilateral - Gram stain
days) lymphadenopathy (extracellular school of
- Multiple lesions - Tender fish)
- Diameter (2-20 mm) - Whitish non foul - Culture media
- Shallow smelling discharge - Clinical diagnosis
- Fever
- Body malaise