Professional Documents
Culture Documents
(serum assay)
agglutination assay
ELISA
precipitation assay
complement fixation test
neutralization assay
Precipitation/Immunodiffusion
nonidentity
Partial
identity
Rocket immunoelectrophoresis
Countercurrent immunoelectrophoresis
Countercurrent immunoelectrophoresis
Double Immunodiffusion
Materials & Equipment
Powdered agar 1 bottle
N S 1
narrow neck flask: one for each group
Balance 1
Electric stove 1
Fridge
Gloves 1
Ear washing bulbs 4
Pipettes 4
Agar slide: one slide for each student
Perforator
Materials & Equipment
Antibody (anti-serum)
Antigens
Positive control (+)
Negative control (-)
Sample 1
Sample 2
Protocols
1 +
Ab
2 -
18-24 hours later
1 +
Ab
2 -
Report 1 : results report
Positive control:
Negative control:
Sample 1:
Sample 2:
OR
To show the result with diagram
Conclusion:
Report 2 : protocol of double immunodiffusion
Concise
Key steps included
Things with more attention
Report 3 : diagrams of radial, rocket and
countercurrent
complement fixation test
MAC erythrocyte
complement fixation test
Neutralization assay
Neutralization assay
Important Notice