Professional Documents
Culture Documents
• Examples:
• Dinoflagellates: Gonyaulax polygramma
Overview 1
• Examples:
– Noctiluca scintillans,
Overview 1
cyanobacterium : Trichodesmium erythraeum
HAB organisms: overview 2
• Species which produce potent toxins that can find their
way through the food chain to humans, causing a variety
of gastrointestinal and neurological illnesses, such as:
• - Paralytic Shellfish Poisoning (PSP)
– dinoflagellates Alexandrium acatenella, A. catenella, A.
cohorticula, A. fundyense, A. fraterculus, A. minutum, A.
tamarense, Gymnodinium catenatum, Pyrodinium bahamense
var. compressum
– Alexandrium Gymnodinium catenatum
Overview 2
• - Diarrhetic Shellfish Poisoning (DSP)
– dinoflagellates Dinophysis acuta, D. acuminata, D.
fortii, D. norvegica, D. mitra, D. rotundata,
Prorocentrum lima )
• Dinophysis Prorocentrum lima
Overview 2
• - Neurotoxic Shellfish Poisoning (NSP)
– Anatoxin Anabaena
Overview 2: Microcystis
single cell colonial cyanobacteria
Toxin: microcystin
Overview 2: Nodularia
Toxin: nodularin
HAB overview 3
• Species, which are non-toxic to humans, but
harmful to fish and invertebrates (especially in
intensive aquaculture systems) by damaging or
clogging their gills.
• Examples:
– Diatom: Chaetoceros convolutus,
– Dinoflagellate: Gymnodinium mikimotoi,
– Prymnesiophytes: Chrysochromulina polylepis ,
Prymnesium parvum, P. patelliferum ,
– Raphidophytes: Heterosigma carterae , Chattonella
antiqua.
Overview 3
• Chaetoceros convolutus
Overview 3
Gymnodinium mikimotoi,
WHY ARE THEY HARMFUL ?
Proliferations of microalgae in marine or brackish waters can cause 1)massive
fish kills, 2)contaminate seafood with toxins, and 3)alter ecosystems in
ways that humans perceive as harmful.
• http://algeinfo.imr.no/
GLOBAL INCREASE OF ALGAL BLOOMS
• Unfortunately, there are very few long term records of algal blooms at any
single locality. Probably the best data set refers to the concentration of PSP
toxins (µg saxitoxin equivalent / 100 g shellfish meat) in Bay of Fundy
(Canada) clams, which has been monitored by mouse bioassay since 1944
(White, 1987). Shellfish containing more than 80 µg PSP / 100 g shellfish
meat are considered unfit for human consumption.
• The Fig. shows evidence for a cyclic pattern of toxicity at this site with
increased frequency of toxic blooms in the late 1940s early 1960s, in the
late 1970s and early 1980s and possibly beginning again in the mid 1990s
GLOBAL INCREASE OF ALGAL BLOOMS
planozygote
hypnozygote
Harmful Algae Volume 14 2012 10 - 35
• Shellfish and in some cases finfish are often not visibly affected by
the algae, but accumulate the toxins in their organs. The toxins may
subsequently be transmitted to humans and through consumption of
contaminated seafood become a serious health threat.
• This has resulted in extensive fish kills with major economic losses. A
comprehensive economic analysis of the global impact of harmful algal
events on the aquaculture industry is not available,
– but the economic losses from single event in North America and
especially Japan have on several occasions amounted to more
than US$ 10 million. On one particular occasion the
raphidophyte flagellate Chattonella antiqua killed US$ 500
million worth of caged fish in Japan.
HARMFUL ALGAE AND MARINE
FOOD RESOURCES
• In developing countries, seafood often constitutes an important or
even sole source of food and protein, especially in costal areas.
• If is not fatal, and the patients usually recover within a few days.
• Ciguatera produces
– gastrointestinal,
– neurological and
– cardiovascular disturbances,
• and recovery often takes months or even years.
Alexandrium
Alexandrium minutum
• Cells of Alexandrium minutum, size between 17
et 29 µm.
The globally distributed genus Alexandrium : Multifaceted roles in marine ecosystems and impacts on human health
http://dx.doi.org/10.1016/j.hal.2011.10.012
lexandrium andersonii ( ), A. minutum ( ), A. tamutum ( ), A. peruvianum/A. ostenfeldii ( ), A. insuetum ( ), A. margalefii ( ), A. pseudogonyaulax ( ), A. taylori ( ), A. affine ( ),
A. catenella Group VI (♦), A. tamarense Group II (□), and Group III (▵).
Carbamoyl Saxitoxins
Name
Registry
R1 R2 R3 R4 Rel.toxicity
Number
Gonyautoxin
60537-65-7 H H OSO3- "
0.64
III
Decarbamoyl 0.51
58911-04-9 H H H OH
saxitoxin
Decarbamoyl
86996-87-4 H OSO3- H " 0.65
gonyautoxin II
Decarbamoyl 0.75
gonyautoxin 87038-53-7 H H OSO3- "
III
Decarbamoyl No data
68683-58-9 OH H H "
neosaxitoxin
Toxity of PSP molecules
Sulfamate Saxitoxins
Gonyautoxin 0.01
89614-45-9 OH OSO3- H "
C3
Gonyautoxin 0.06
89674-98-6 OH H OSO3- "
C4
Saxitoxins: characteristics and mode of
action
• This toxin blocks neuronal transmission by binding to the voltage-
gated Na+ channels in nerve cells, thus causing their neurotoxic
effects.
• STXs are highly toxic, killing guinea pigs at only 5 µg/kg when
injected i.m.
• The lethal doses for mice are very similar with varying adminstration
routes: i.p. (LD50 = 10 µg/kg), i.v. (LD50 = 3.4 µg/kg) or p.o. (LD50
= 263 µg/kg).
• Saxitoxin can enter the body via open wounds and a lethal dose of
0.05 mg/person by this route has been suggested. Saxitoxin is 1,000
times more toxic than the potent nerve gas sarin
Saxitoxins: characteristics and mode of action (f)
• Saxitoxin is a potent neurotoxin that specifically and selectively binds the
sodium channels in neural cells. Thus, it physically occludes the opening of
the Na+ channel and prevents any sodium cations from going in or out of
the cell. Since neuronal transmittance of impulses and messages depends
on the depolarization of the inside of the cell, the action potentials are
stopped, impairing a variety of bodily functions, including breathing.
• Human nerves are especially sensitive to the toxins and in the early stages
of PSP, victims experience
– tingling and numbness of the mouth, tongue, face and extremities. Nausea and
vomiting may accompany the above symptoms.
– In severe cases, the patient will exhibit advanced neurological dysfunction such
as ataxia, weakness, dizziness, numbing of the lips, mouth and tongue, fatigue,
difficulty breathing, and sense of dissociation followed by complete paralysis.
The diaphragm may stop working and death can occur after cardio-respiratory
failure. Symptoms occur between 10 minutes and four hours after ingestion,
depending on the dose.
•
Identification of saxitoxin as a cause of intoxication by virtue of clinical
symptoms is not simple, because faulty identification of this toxin as nerve
gas poisoning may be fatal and administration of atropine would increase
fatalities. The laboratory detection and identification of compound is difficult
and is possible only in a well-provided analytical laboratory.
Dinophycae (= Dinoflagellates):DSP
Prorocentrum lima,
Prorocentrum elegans,
Prorocentrum offmannianum
Prorocentrum concavum,
Dinophysis acuminata,
Dinophysis acuta,
Dinophysis fortii, (pectenotoxin)
Dinophysis hastata,
Dinophysis mitra,
Dinophysis rotundata,
Dinophysis norvegica and some strains
of Dinophysis tripos.
Protoceratium reticulatum (Yessotoxin) Dinophysis
DSP molecules
is a complex
lipophilic polyether
readily soluble in
many organic
solvents, degrading
in acid or base.
Okadaic group
DSP
• Diarrhetic shellfish poisoning (DSP) was first reported from the Tohoku
district in Japan.
• Since then, reports of DSP have emerged from every continent except
Africa and Australia.
• DSP has never resulted in a human fatality.
• Apart from this acute effect, chronic exposure may promote cancer as it
enhances skin tumours on mice when applied after a known carcinogen.
Mode of action of okadaic acid
Pecteno
toxin
group
DSP
Yesso-
toxin
group
Aza-
spiracids
Dinophycae (= Dinoflagellates):NSP
Gymnodinium breve
NSP molecules: brevetoxin
brevetoxin
Brevetoxin (f)
• Brevetoxins (BVX) are neurotoxins produced by algae called Karenia brevis
(also called Ptychodiscus brevis and formerly Gymnodinium breve) from
which the toxin name is derived. The algae proliferate during red tide
incidents.
• Brevetoxins and related toxins are believed to have been responsible for
massive fish kills from red tides in several regions. A long history of toxic
microalgal blooms exists in the Gulf of Mexico, blooms that have caused
massive fish kills and respiratory irritation in humans.
• It was later realized that the toxin in these blooms could also be passed to
humans via shellfish to cause a syndrome named neurotoxic shellfish
poisoning (NSP). Until cases were reported in New Zealand and Australia in
the early 1990s, reports of NSP were limited to the Americas.
These toxins depolarize and open voltage gated sodium (Na+) ion channels in
cell walls, leading to uncontrolled Na+ influx into the cell . Brevetoxins bind
to the ion channels of nerve and muscle tissue that selectively allows
sodium to pass into the cell. These sodium channels open during an action
potential in response to the change in the electrical potential across the cell
membrane. Brevetoxins change the voltage at which this opening occurs
nearer to the voltage threshold that triggers this process essentially making
the sodium channel, and consequently, the affected nervous and muscular
cells hyperexcitable.
Brevetoxins are unusually stable materials in the dry state. They are stable as
well as in different solvents (acetone, acetonitrile, alcohol, ethyl acetate or
DMSO), including water, where half-lives for active material range from 4-6
months. Solutions with a pH lower than 2 or higher than10 degrade the
toxins.
CFP
• Ciguatera is caused by Gambierdiscus
toxicus, and
• perhaps also by some species of
Ostreopsis and Prorocentrum. Most of
these species can readily be identified by
trained taxonomists.
Gambierdiscus toxicus
Characterising algal blooms:
species and toxins involved, CFP
Ostreopsis
CFP (f)
• The term ciguatera originated in the Caribbean area to designate
intoxication induced by the ingestion of the marine snail Turbo pica (called
cigua), first described by a Cuban ichthyologist.
• Today, the term is widely used to denote a particular type of fish poisoning
that results from ingestion of primarily reef fishes encountered around
islands in the Caribbean and the Pacific.
• Current information points to one of the many polyether toxins, such as
ciguatoxin and related compounds, which are structurally similar to okadaic
acid.
• Ciguatoxin is produced by the Dinoflagellate Gambierdiscus toxicus and has
been isolated from the flesh and viscera of ciguatoxic fish.
Pseudo-nitzschia australis
Pseudo-nitzschia multiseries
ASP
• Domoic acid (DA), was originally isolated from the
macroscopic red alga Chondria armata, locally known in
Japan as domoi.
• Human intoxications
– PSP known since 1793
– DSP known since 1978
– NSP known since 1970s
– ASP known since 1987
– CFP known since 16th century
Basic type of phytoplankton toxins
• Water soluble toxins
– Saxitoxin family (causing PSP, at least 18 forms)
– Some amino acids (domoic acid, mycosporine-like
amino acids
• Fat soluble toxins
– Okadaic acid and its derivatives
– pectenotoxins, DSP
– Polyethers: brevetoxins (NSP), ciquatoxin, ciguatera
– Yessotoxine
– Fatty acid and glycolipids
– Peptides (hepatotoxins from cyanobacteria)
HAB and impact on shellfish
• The potential of direct impact of toxin is of great concern
• Some toxins however have only minor effects on shellfish physiology
• Other (including uncharacterised) seem to have a bigger impact
• Karenia mikimotoi:
• Has been associated with direct killing of pearl oysters
• In Mytilus galloprovencialis:
• Reduced filtration rate when exposed to 5x 105 cell per ml
• Death at 5 x 106 per ml
• No mode of action has been establised
Domoic acid and fish behavior
• Research and field observations have provided evidence that fish are more
tolerant to domoic acid under ecologically relevant exposure conditions than their
piscivorous predators.
• This is an important distinction as more attention has been drawn to domoic acid
producing algal blooms and the potential for domoic acid to cause fish kills.
• Currently available data indicate that domoic acid producing algal blooms do not
cause fish kills or neuroexcitotoxic behaviors in fish.
• Neuroexcitatory behavioral effects have been documented in fish in laboratory studies
when fish were intraceolomically (IC) injected with domoic acid. In fact, with IC injection
as the mode of exposure all fish, bird, and mammal species tested to date show a similar
neurologic sensitivity to domoic acid in terms of behavioral excitotoxicity as quantified
by a 50% effective concentration (EC50) metric.
– For example, there are both toxic and non-toxic variants of the
dinoflagellate Alexandrium tamarense.
• Differentiation
– between the toxic A. tamarense/A. catenella and the non toxic A.
fraterculus (Cho et al., 1999)
• Remark:
– Binding can be growth phase specific, strain specific (e.g. diatoms),
– in other species it is constant (some dinoflagellates and cyanobacteria)
– HENCE NEED TO VALIDATE
Lectin-aided detection
In this case Gymnodinium was stained with FITC –labelled lectin from
Phaseolus limensis (bean) (excitation 490 nm, emission 520 nm
FITC
ID tools: antibodies
• The approach involves the use of antibodies that bind
specifically to proteins in the cell walls of the algal
species of interest.
• The antibody for the brown-tide organism has been used for
– cell enumeration and grazing studies, and
– to map the geographic distribution of this species over a large region
(Anderson et al., 1993). This antibody is now used for routine monitoring
of this harmful species.
– A MAb to the same organism is now available, as well, and it is being
used for whole cell assays and in a semi-automated ELISA plate format
Antibodies
• Finally, a MAb has been applied in field studies of Alexandrium
tamarense in the Gulf of Maine, USA (Townsend et al., 2001).
• Finally, the use of magnetic beads with cell surface antibodies offers
the possibility of cell separation and thus species-specific
physiological measurements.
– There is thus sufficient benefit and potential for antibodies that
development efforts should be continued, in parallel with development
of oligonucleotide probe technologies.
NA probes
• In recent years, use of nucleic acid probe technology to detect
microorganisms has expanded considerably.
• Typically,
– target genes have sequence domains that are highly conserved among
all organisms, and that are thus not useful in discrimination,
– as well as other domains that are variable to different degrees. It is the
latter that are the target areas for probe development.
• In this “whole cell” format, the probe enters the cell and
binds to target sequences, excess probe is washed out,
and the complex is detected by fluorescence or
radioactivity.
• Standardized strains of laboratory mice of defined age, sex and weight are
frequently used in phycotoxin bioassays.
• Survival time of mice is generally used for the measurement of global toxicity,
expressed in mouse units (MU) which are converted into toxin specific units
(e.g. saxitoxin equivalents [STXeq]) based upon the toxicity response
calibrated with reference to toxin standards.
•
Mouse assay: advantages
• The principal advantage of a well administered and properly
calibrated mammalian bioassay, compared to physico-chemical
analysis or many in vitro methods, is that the toxicity determination
is directly relevant to human toxicity effects.
– due mainly to a number of variables that can affect the results, such as
specific animal characteristics (strain, sex, age, weight), general state of
health, diet, stress conditions, pH of the injected extracts, etc.
• For example, for the AOAC mouse bioassay for PSP toxicity, the
acceptable regulatory limit for human consumption of shellfish
adopted in many countries (80 µg STXeq/100 g soft tissue) is only
twice the nominal toxicity detection limit (cu. 40 µgSTXeq/100 g). This
provides little security margin for technical errors. Moreover, no
definitive qualitative information is provided on the nature of the toxin
components. (AOAC:Association of Official Agricultural Chemists, US
funded organisation dedicated to analytical excellence, now changed
to analytical chemists)
Mouse assay: disadvantages
• This is particularly a problem for samples which contain multiple toxin
analogues which vary in specific toxicity, or where the co-occurrence of
different phycotoxins can lead to synergistic or antagonistic biological
responses, e.g. the simultaneous presence of a Na+ channel activator and a
blocker.
• The PSP toxins are least stable at alkaline pH, yet heating under
strongly acidic conditions (e.g., pH 2) can also lead to chemical
transformation, with the degree of conversion depending upon the pH
(Nagashima et al., 1991). Between pH 3 to 4, all PSP components are
in a range of optimal stability. Low pH of the injected extract can also
lead to mouse bioassay artifacts caused by acidosis.
ASP mouse assay
• The AOAC mouse bioassay for PSP toxins can also detect domoic acid at
concentrations ca. 40 ppm and this procedure was used when ASP toxicity
was first identified in Canada in shellfish extracts from eastern Prince
Edward Island (Wright et al., 1989).
• For the routine detection of ASP toxins, the AOAC mouse bioassay has
been superseded by HPLC methods using diode-array/UV or fluorometric
detection (Quilliam et al., 1989; Pocklington et al., 1990) which have been
proven to be more sensitive and reliable tools
DSP mouse assay (reading)
• There is no general agreement concerning the appropriate testing
procedures to be applied for DSP toxins for regulatory purposes, and
standardization of methods represents a difficult administrative and
scientific challenge.
• One of the main problems arises from the different biological activity of the
three groups of lipophilic toxins currently included in the DSP toxin
complex (Yasumoto, 1990) –
– okadaic acid (OA) and
– analogues such as DTXl,
– DTX2, and
– acyl-derivatives (DTX3),
– polyether lactone pectenotoxins (PTX), and
– yessotoxins (YTX), disulfated polyether compounds.
• In the last few decades, there have been several concerted attempts to
produce reliable immunodiagnostic test kits for various phycotoxins.
Detecting toxins
• Many of these efforts have been hampered by
• The microtiter wells are coated with antibodies directed against saxitoxin
loaded antibodies
• Cross-reactivity:
• Saxitoxin .........................................................100 %
• Decarbamoyl saxitoxin .............................. 10 - 30 %
• Gonyautoxins II, III, B1, C1 und C2.............. 10 - 30 %
http://www.jellett.ca/global_occurrences.htm
Mist Alert system for
PSP:
vomiting,
nausea, diarrhoea,
vomiting, tingling, hot and abdominal pain, confusion,
acute symptoms diarrhoea, paralysis diarhoea cold, dizziness nausea memory loss
Glutamate
Phosphorylase receptor
Toxin molecular mechanism Na channel blocker inhibitor Na channel activator Na/ Ca activator agonist
below detection
Legal limit in EU 80µg/100g limit 160µg OA/kg 20µg/g
Yessotoxin: 1mg/kg
Azaspiricid:
160µg/kg
Latest EU regulations
• Regulation (EC) No 853/2004
• establishes that food business operators must ensure that live bivalve
molluscs placed on the market for human consumption must not contain
marine biotoxins in total quantities (measured in the whole body or any part
edible separately) that exceed the following limits:
• • 800 micrograms per kilogram for paralytic shellfish poison (PSP):
• • 20 milligrams of domoic acid per kilogram for amnesic shellfish poison
(ASP):
• • 160 micrograms of okadaic acid equivalents per kilogram for okadaic acid,
dinophysistoxins and pectenotoxins in combination:
• • 1 milligram of yessotoxin equivalents per kilogram for yessotoxins:
• • 160 micrograms of azaspiracid equivalents per kilogram for azaspiracids.
The ecology of algal blooms
• species appear to be stimulated by “cultural
eutrophication” from domestic, industrial and agricultural
wastes.
–
The ecology of algal blooms
The ecology of algal blooms
– A similar experience was noted in the Seto Inland Sea, one of
the major fish farm areas in Japan (Okaichi, 1989).
– Between 1965 and 1976 the number of confirmed red tide
outbreaks (mainly Chattonella antiqua, since 1964; and
Gymnodinium nugusukiense, since 1965)
• progressively increased 7-fold,
• concurrent with a 2-fold increase in the COD (chemical oxygen
demand) loading, mainly from untreated sewage and industrial
waste from pulp and paper factories.
– During the most severe outbreak in 1972, a Chattonella red tide
killed 14 million cultured yellow-tail fish.
• Effluent controls were then initiated
– to reduce the chemical oxygen demand loading by about half,
– to introduce secondary sewage treatment, and
– to remove phosphate from house-hold detergents.
– Following a time-lag of 4 years, the frequency of red tide events in the
Seto Inland Sea then decreased by about 2-fold to a more stationary
level.
The ecology of algal blooms
Time series of nutrient and Pseudo-nitzschia in the
Mississipi delta. A) nitrate nitrogen increase. B)
densities of Pseudo-nitzschia
The ecology of algal blooms
• A similar pattern of a long-term increase in nutrient loading of
coastal waters is evident for the North Sea in Europe (Smayda,
1990) .
– Since 1955 the phosphate loading of the River Rhine has increased 7.5-
fold, while nitrate levels have increased 3-fold. This has resulted in a
significant 6-fold decline in the Si:P ratio, because long-term reactive
silicate concentrations (a nutrient derived from natural land weathering)
have remained constant.
– More recently, improved wastewater treatment has been causing
decreases in the ammonia:nitrate ratio of River Rhine discharge
(Riegman et al., 1992). The nutrient composition of treated wastewater
is never the same as that of the coastal waters in which it is being
discharged.
• Enhancement factors:
– cell multiplication can be encouraged by hydrological and climatic
conditions.
– It has been recognized that blooms appear in warm and stable waters
where mixing is absent; and after heavy rain fall, not because of a drop
in salinity but because of stratification.
– This stability of the water column is important because it favours the
accumulation of algae at the optimal depth for growth.
– Wind-driven currents, tides, upwelling, downwelling, divergencies and
convergencies as well as other frontal boundary structure may be
prerequisited for blooms to occur.
– While nuisance blooms are virtually always coastal phenomena, it has
been suggested that favourable physical and hydrological conditions
must act synergistically with nutrient enrichment to yield maximum
biomass.
– Allochtonous and terrigenous nutriet loadings are almost invariably
linked to nuisance bloom developments.
Development of blooms
• While dinoflagellates can develop in nutrient-poor
waters, diatoms require waters with high concentrations
of nutrients. This means that diatoms consume more
nutrients than dinoflagellates, and the high division rate
of diatoms allow them to outcompete dinoflagellates.
• However, some dinoflagellates are known to grow in
nutrient-rich waters, e.g. Prorocentrum
Blooms in relation to hydrography
• In the North west of Spain there are four bays (rias) linked with
rivers that are rich in nutrients. Hence together with local
hydrological features (upwelling) the rias themselves are loaded with
nutrients. Because of this they support immense mussel, oyster and
fish culture. Ria de Vigo is one of the four oceanic bays which
support a major raft-suspension blue mussel aquaculture industry in
Spain ( more than 200 000 ton)
Blooms in relation to hydrography
• In this area, there are intensive upwelling periods,
especially during summer. It is observed that blooms
occur between July and October (late summer/autumn)
when upwelling is weak or absent and when a mixture of
autotrophs and heterotrophs occur.
• When upwelling occurs, jets of cold North Atlantic waters
are injected into the rias. This lowers the water
temperature in the ria, while it is warmer offshore.
• Intense upwelling favours the development of diatoms.
This is because strong upwelling destroys stratification
which allows nutrients from the bottom to be loaded into
the photic zone.
Blooms in relation to hydrography
Blooms in relation to hydrography
• When the upwelling ceases in autumn, offshore
water flow into the rias.
• This increases the temperature of the water in the
rias.
• The inflow of warm waters coincides with very
weak upwelling ( which drives the nutricline below
the photic layer) and favours the development of
flagellates.
• With the nutricline pushed into the deeper waters,
out of the photic zone, It is only the actively
swimming dinoflagellates (like the chain forming
Gymnodinium catenatum) which are better
adapted to vertical migration, that can penetrate
the deep waters and take up nutrients and flourish
to the disadvantage of diatoms.
Blooms in relation to hydrography
Ria’s in Galicia
Transport of Dinophysis along and into the coasts of north-western Europe. (A–C) The system of currents
along the north-western Atlantic coast of the Iberian peninsula illustrated by Escalera et al. (2010). Grey thick
arrows are the poleward counter current of the Eastern Boundary Current system. (A) In summer the
Portuguese Coastal Current (long black arrows) flows south, promoted by upwelling favourable northerly
winds. Cross shelf transport of plankton arises during upwelling relaxation (thin grey arrows). (B) During
autumn a narrow inshore flow of warm water is found (dotted lines) and transports harmful dinoflagellates
north towards the Galician Rías (NW Spain). This prevails during the whole autumn–winter season except
when (C) upwelling-favourable winds blow. (D) A baroclinic coastal flow occurs around the perimeter of the
Celtic Sea (Hill et al., 2008) in summer. This takes the form of strong, narrow jets which are found over bottom
fronts (see inset in D) and transport plankton along the coastline. The alongshore flows transport plankton
populations towards aquaculturally important bays, indicated in (C) and (D). Exchanges of water and plankton
between the coastal shelf and bays (E) are caused by downwelling, whereby populations including Dinophysis
are carried into the bays with warm surface water during upwelling relaxation (Iberia) or wind-forcing from the
predominantly south-westerly winds that occur in south-western Ireland. The dynamics are maintained by flow
reversal (lower diagram) caused either by resumption of upwelling favourable winds (Iberia) or by winds
blowing axially along the bays towards the open ocean (southwest Ireland).
Case Study
Interannual variability of (a) temperature (°C), (b) salinity (‰), and (c) Alexandrium tamarense cell
abundance (cells·L–1) at Sept-Îles between mid-May and the end of October, 1989–1998. Data
were only available as of mid-June 1990 and mid-July 1989 and 1992. The dotted line at 1000
cells·L–1 represents the concentration at which shellfish generally become toxic in the St.
Lawrence (80 μg STX eq·100 g meat–1).
Case study
• A. tamarense cell
concentrations (cells·L–1) during
(c) 1996 and (f) 1992. Salinity
and cell abundance data were
only available as of mid-July
1992.
Case study
• Association between
heavy rainfall, river
runoff, salinity, and
Alexandrium tamarense
blooms at Sept-Îles
during 1992 and 1996.
• Daily Moisie River runoff
(m3·s–1; solid line) and
rainfall (mm; bars) during
(a) 1996 and (d) 1992;
• salinity (‰) during (b)
1996 and (e) 1992;
• A. tamarense cell
concentrations (cells·L–1)
during (c) 1996 and (f)
1992.
• Salinity and cell
abundance data were
only available as of mid-
July 1992
Case study
Case study
Environmental conditions
preceding the 1994
sustained bloom at Sept-
Îles. (a) Daily Moisie
River runoff (m3·s–1)
(solid line) and rainfall
(mm) (bars), (b) salinity
(‰), (c) wind speed (m·s–
1), and
• The fact that significant interannual variability in both the timing and
magnitude of A. tamarense blooms is observed over the 10-year
period demonstrates that this proper combination of du Saint-
Laurent factors is not met every year.
• The REPHY network contains a series of sampling sites distributed over the
entire French coast.