COLLIGATIVE PROPERTIES

• Properties of solutions that depend on the

ratio of the number of solute particles to the
number of solvent molecules in a solution,
and not on the nature of the chemical species
present.
• Include vapor pressure lowering, boiling point
elevation, freezing point depression, and
osmotic pressure.
Derivatization-free Gel Permeation
Chromatography Elucidates
Enzymatic Cellulose Hydrolisis
Autors:
Philip Engel
Lea Hein
Antje C. Spiess
 ABOUT THE AUTORS:
• Philip Engel:
AVT-Enzyme Process Technology, RWTH Aachen University,
Aachen, Germany

• Lea Hein:
AVT-Enzyme Process Technology, RWTH Aachen University,
Aachen, Germany

• Antje C Spiess:
AVT-Enzyme Process Technology, RWTH Aachen University,
Aachen, Germany
Interactive Materials Research, Aachen, German
 IMPORTANCE OF THE ARTICLE

• Work and time consuming sample

preparation, the measurement of
cellulose molecular weight distribution
has a limited applicability until now.
EXPERIMENTAL PROCEDURE
 RESULTS
• New method to analyze cellulose molecular
weight distributions that does not require any
prior cellulose swelling, activation or
derivatization. The cellulose samples were
directly dissolved in DMF containing 10-20%
(v/v) EMIM Ac for 60 min. Reducing the
sample preparation time.
 CONCLUSIONS
• This strongly simplified GPC method for
cellulose molecular weight distribution
allowed for the first time to demostrate the
influence of cellulose properties and
pretreatment on the mode ofenzymatic
hydrolysis.
 REFERENCES
• Chem.fsu.edu. (2016). Colligative Properties.
[online] Available at:
https://www.chem.fsu.edu/chemlab/chm104
6course/colligative.html