GENETIC ASPECT OF FOLLICULOGENESIS

Budi Wiweko
wiwekobudi@yahoo.co.id

FKUI Klinik Yasmin RS Dr. Cipto Mangunkusumo

 “Y” makes you male
Individuals with a Y chromosome almost always have a male phenotype
 Sertoli cell
activity Leydig cell activity

Testis descent

AMH External Genital Growth

Male External Genital
Differentiation

Wollfian Duct Development

Germ Cell Migration Mullerian Duct Regression

4 5 6 7 8 9 10 11 12 13 14 15 40
Gestation (weeks)

Sex determination Sex differentiation

Hughes. Endocrinology, 2001

 8th-15th weeks

Speroff L.Clinical Gynecologic Endocrinology and Infertility, 7th, 2006

Yen & Jaffe's Reproductive Endocrinology, 6th Edition. 2009
Except for phallic growth and inguinoscrotal descent in the third trimester,
male sexual differentiation is essentially complete within 15 weeks of
gestation

Sharpe RM. Best Pract Res Clin Endocrinol Metab, 2006

MacLaughlin D. N Engl J Med 2004;350:367-78
46, XX DSD

46, XY DSD

MacLaughlin DT. New Eng J Med, 2004

 1………..Filosofi

AMBANG RANGSANG FSH

1. Pertumbuhan folikel membutuhkan stimulasi FSH yang
melewati ambang rangsang

2. Kadar FSH yang melewati ambang rangsang, akan memicu

proliferasi sel granulosa, aktifitas enzim aromatase dan
pembentukan reseptor LH di sel granulosa

3. Folikel yang terseleksi dan tumbuh akan semakin meningkat

sensitifitasnya terhadap FSH

Brown, 1978
 is member of transforming growth factor β

Sertoli cells Primordial follicles

36th week of pregnancy
sex differentiation

Mullerian duct regression Inhibits follicle recruitment

Vigier et al. Production of anti Mullerian hormone: another homology between Sertoli and
granulosa cells. Endocrinology, 1984
 Correlation between
FSH Receptor Polymorphism and Ovarian response
FSH Receptor Polymorphism

Asn / Asn Asn / Ser Ser / Ser p

n % n % n %

Good Response 31 67.8 27 70.4 7 70 0.165*

Poor Response 9 32.2 8 29.6 3 30

Total 40 100 35 100 10 100

*Kolmogorov-Smirnov Test Poor responder:

n = 85
Allele Ser = 14/55 = 18%
Allele Asn = 26/115 = 22%

Wiweko et al, 2011. In preparation for publication

n = 85
Ser/Ser Asn/Asn Ser/Asn

Wiweko et al, 2011. In preparation for publication

Folikulogenesis

Tahap maturasi
Introduction

Oogenesis
and
folliculogenes
is

Menstrual
cycle

Take home
messages
 Acknowledgement
Ali Baziad, Andon Hestiantoro, Muharam Natadisastra, Kanadi Sumapraja,
Gita Pratama, Herbert Situmorang Eliza Mansyur, Tita Yuningsih, Dyah Pitha, Siti Mariam, Endang Kurdiningsih,
M Priangga, Valencia Yuwono, Nadia Safira

Indonesian Reproductive Medicine

Research and Training Center (INA – REPROMED)