BIOTEKNOLOGI

Gabriela Welma Litaay 15/389567/PMU/08526 S E K O L A H PA S C A S A R J A N A

Yenny Niken Larasati 15/389586/PMU/08545 UNIVERSITAS GADJAH MADA
2016
Transgenic animals??
Sosa et al., 2010
 Applications of Transgenic Animals

Health Agriculture Environment

• Recombinant Therapeutic • Meat Production • Pollution Indicator
Protein • Growth Rate
• Blood Substitute • Milk production
• Antibody • Fiber Production (By-Product)
• Feed Conversion
• Xenotransplantation
• New Habitat
• Human Disease Model • Disease Resistant
• Reproductive Performance
 Applications of Transgenic Animals : Health
1. Recombinant Therapeutic Protein

(Rajoriya et al., 2013)

Applications of Transgenic Animals : Health
2. Blood Substitute
Production of functional hemoglobin from swine
which has the same oxygen binding capacity as that of
normal human hemoglobin and that could be purified
from porcine blood.
(Chang and D’Agnillo, 1988)
Applications of Transgenic Animals : Health
3. Antibody
 Production of human
monoclonal antibodies
from human Ig translocus
mice (Bruggemman, 2001).
 Production of human
polyclonal antibodies from
calves and goat (Kuroiwa
et al., 2002).
(Pollock et al., 1999)
Applications of Transgenic Animals : Health
4. Xenotransplantation
Living cell/ tissue/ organ would be
transplanted from animals into humans until
human replacement organs become
available.
 Use pig islets to boost insulin production in
patients with type-I diabetes (Fabregas,
2006).
 Use pig hearts for patients with heart
failure rather than using mechanical
pumps (Fabregas, 2006).
 Applications of Transgenic Animals : Health
5. Human Disease Model
Animal Disease
Mouse HIV/AIDS
Alzheimer’s disease
Cardiovascular disease
Diabetes Mellitus
Angiogenesis
Cancer disease

(Blundell, 2006)
Applications of Transgenic Animals: Agriculture
Applications of Transgenic Animals: Agriculture

Transgenic Tilapia Transgenic Atlantic Salmon

Applications of Transgenic Animals: Agriculture
Applications of Transgenic Animals: Agriculture
Applications of Transgenic Animals: Agriculture

Reproductive Performance

booroola Improved Sheep Nimbkar et

fecundity productivity al., 2005,
gene (FecB) (prolificacy) Gootwine,
1995
gene from Enhanced estrus Pig Seidel, 1999
baboons
estrogen increased the Pig Rothschild et
receptor litter size al., 1994
gene (ESR)

(Rajoriya et al., 2013)

Applications of Transgenic Animals : Environment
Pollution Indicator
a. Fish
 inserted GFP into a zebrafish embryo
 develop a fish that could detect pollution
by selectively fluorescing in the presence
of environmental toxins
(Dr. Zhiyuan Gong, 1999)

b. Frog
 African Clawed Frogs (Xenopus laevis)
were used as endocrine disrupting
chemicals in wastewater
Ethical Issue of Transgenic Animals : Cons

great suffering to the animals

reduce them to mere factories

animals have the same basic rights as human beings

animals have a right to exist as a separate identifiable entity

the introduction of human genes into animals  not

“humanness”
Background

hG-CSF for
therapeutic
purposes

Limitation
of
transgenic
cattle Transgenik
goats for
mammary
gland hG-CSF
expression
Material and Methods

• Animal Ethics & Biosafety

• Animals
Preparation
• Pronuclear embryo production

• DNA microinjection and embryo transfer

Transforma
tion

• Transgene Detection
• Western blotting analysis
Detection
• Transgene transmission to progeny
Cont…
Result
2. The detection of the hG-CSF gene in the
1. identification of hG-CSF transgenic goats
using PCR analysis. spermatozoa of the 10 M transgenic founder
using PCR analysis.

M 10M nonT 12F C (-) T M

M T nonT T nonT C (-) M
Semen Semen Skin Skin
Result
The expression of hG-CSF in the milk of the 12 The colony-forming assay performed on human
F female transgenic goat. umbilical cord blood.

M T nonGlyc nonT T nonGlyc nonT

hG-CSF hG-CSF

PCR Western Blotting

Result
Result
Result

Analysis in light microscopy of the colonies Transgene transmission to the offspring from
formed after addition of transgenic goat milk. two hG-CSF transgenic goat lines.

hG-SCF hG-SCF hG-SCF

50 ng/mL 100 ng/mL 200 ng/mL
 Conclusion
• Two transgenic goats with a stably integrated hG-CSF gene

* were capable of secreting recombinant hG-CSF from the

lactating mammary gland without causing any harm to the
animals’ health and transmitting hG-CSF to first generation
progeny of each line.

• Aplications of transgenic animals are for health

(recombinant therapeutic protein, blood substitute,
antibody, xenotransplantation, human disease model),
* agriculture (meat production, growth rate, milk production,
fiber production, feed conversion, new habitat, disease
resistant, reproductive performance) and environment
(polution indicator).