Use of the Labnet Micropipettor

Purpose of the Pipettor

• Biotechnologists use a
pipettor to transfer
precise amounts of
liquids from one
container to another.
• The measurements are in
microliters 1 ml=1000 µl.

Use of the Labnet Micropipettor

 Care of the Pipettor

• Misuse of the pipettor can

result in incorrect
measurements affecting
your investigation.
• Do not drop the pipettor.
• Do not adjust the volume
to less than 2 µl.

Use of the Labnet Micropipettor

 Parts of the Pipettor
1
1
2
6
3
• 1) yellow pushbutton 6
6

• 2) calibration knob 3

• 3) digital volume display

• 4) barrel 4
• 5) disposable pipette tip 4
• 6) tip ejector
5
5

Use of the Labnet Micropipettor

 Volume Adjustment
Step 1

• In the display window are

three vertical numbers.
• The top number is tens µl
(10.0).
• The middle number is
ones µl (02.0).
• The bottom number
tenths µl (00.9).

Use of the Labnet Micropipettor

 Volume Adjustment
Step 2
• Set the pipettor to the desired
volume by turning the
calibration knob until the
volume display reads the
desired numbers.
• 7.5 µl would read from top to
bottom in the display window:
0 - tens
7 - ones
.5 - tenths

Use of the Labnet Micropipettor

 Transferring Liquids
Step 1

• Adjust the pipettor to the

desired volume.
• Hold the pipettor so the
display is facing the user
and the thumb can easily
depress the yellow
pushbutton.

Use of the Labnet Micropipettor

 Transferring Liquids
Step 2

• Open the box of yellow

pipette tips supplied by the
Office of Biotechnology.
• Place the pipette cone (end)
into one of the tips and
press down firmly.
• NEVER USE THE
PIPETTOR WITHOUT A
TIP!
Use of the Labnet Micropipettor
 Transferring Liquids
Step 3

• Lift the pipettor and

attached tip away from the
tip box.
• Depress the pushbutton to
the first stop before
placing the pipette tip in
the liquid.
• Place the tip in the liquid.

Use of the Labnet Micropipettor

 Transferring Liquids
Step 4

• Slowly release the

pushbutton and draw the
liquid into the tip.
• Remove the tip from the
liquid and visually check
for liquid in the tip.

Use of the Labnet Micropipettor

 Transferring Liquids
Step 5

• Place the tip into the

container which is to
receive the liquid.
• Slowly depress the
pushbutton as far as it can
go.
• With the pushbutton fully
depressed, withdraw the
tip.

Use of the Labnet Micropipettor

 Transferring Liquids
Step 6

• Press down on the tip

ejector button to eject the
tip.
• Dispose of the tip in an
appropriate container.

Use of the Labnet Micropipettor