An in-vitro Comparative Evaluation of

Antimicrobial Efficacy of
Electrochemically Activated Water and
Super Oxidised Solution as Root Canal
Irrigant.

GUIDED BY : PRESENTED BY:

DR. SANJEEV TYAGI RITU GUPTA
DR. ABIN MANI MDS 1ST YEAR
 INTRODUCTION AND NEED FOR THE STUDY
A variety of micro-organisms in root canal are
accountable for the infections of pulp and periradicular
infections. Enterococcus faecalis is majorly isolated from
failed root canals. Its ability of invasion in dentinal
tubules and independence of other bacteria’s survival
makes it leading bacteria in terms of resistance to various
endodontic treatment procedures.
 Sodium hypochlorite though used widely, has drawbacks
like toxicity to living tissues and causes pain, swelling,
necrosis when extruded through apices. Also, it has
corrosive nature which makes the ultrasonic units used in
canal irrigation prone to mechanical breakdown
 Readily available Super Oxidised Solution (SOS) is used
in general medicine and due its properties can be
checked for its use as root canal irrigant. It’s
composition is hypochlorous acid 0.003% w/v, oxidised
water 99.97w/v and sodium hypochlorite 0004 %w/v.
Also, it has better shelf life (upto 2 years) compared to
other prepared electrolytic water.
 Earlier, Electrochemically Activated Water (EAW) was
prepared using platinum and titanium electrodes, we
aim to also use silver electrodes as it has antimicrobial
properties and graphite electrodes as it is comparatively
cost effective in addition to the inertness of platinum.
 HYPOTHESIS :

The study is being conducted under null hypothesis that there would be no
significant difference in the antimicrobial efficacy produced between the
groups

AIM
 The aim of present in vitro study is to assess and
comparatively evaluate the antimicrobial efficacy of EAW
prepared using platinum, silver, graphite electrodes and
commercially available Super Oxidised Solution (SOS) with
contemporary irrigant sodium hypochlorite, in human root
canals infected with E.faecalis.
 OBJECTIVES
 To estimate anti-microbial efficacy against E.faecalis
of EAW prepared using
i) platinum electrodes
ii) silver electrodes
iii) graphite electrodes
 To determine anti-microbial efficacy of SOS against
E.faecalis
 To determine anti-microbial efficacy of sodium
hypochlorite against E.faecalis
 To determine anti-microbial efficacy of normal saline
against E.faecalis
 To compare anti-microbial efficacies of all EAW(s) and
SOS against sodium hypochlorite
 METHODOLOGY
STUDY
• Experimental study
TYPE

• Freshly extracted non-carious 60 mandibular

premolars to be collected form Department of
SOURCE OF
DATA Oral and Maxillofacial Surgery, PDA, Bhopal

• 60 human sound mandibular premolars with

SAMPLE complete root formation
SIZE
 Experimental groups
The teeth will be divided into 6 groups :
 Group A (n=10)- Root canal irrigation with EAW
prepared using platinum electrodes
 Group B (n=10)- Root canal irrigation with EAW
prepared using silver electrodes
 Group C (n=10)- Root canal irrigation with EAW
prepared using graphite electrodes
 Group D (n=10)- Root canal irrigation with SOS
 Group E (n=10)- Root canal irrigation with sodium
hypochlorite solution
 Group F (n=10)- Root canal irrigation with normal
saline
 MATERIALS:
 Sodium hypochlorite
 SOS
INSTRUMENTS:
 Normal saline
• Air rotor
 Distilled water
• Round bur
 Glass ionomer cement
• Gates Glidden burs
(GIC)
• K-type files(size 6-60)
 Calcium hydroxide
• Syringe
powder
• Paper points
 Zinc oxide eugenol (ZOE)
• pH paper
 Semipermeable dialysis
tubing membrane
 Platinum electrode (2)
 Silver electrodes(2)
 Graphite electrodes(2)
 Power source EQUIPMENTS:
 Ammeter
 Voltmeter • Biological Oxygen Demand (BOD) incubator
 Brain Heart Infusion • Digital Colony Counter
broth • pH meter
 Enterococcus faecalis • Spectrophotometer
culture
 Blood agar plates
 EXPECTED OUTCOME
 This study would help us figure out better root canal
irrigants alternative to sodium hypochlorite while
having the same advantages of sodium hypochlorite
and overcoming its drawbacks of storage risks and
toxicity caused when extrusion through root apex
occurs.
 BIBLIOGRAPHY
 Akbulut MB, Eldeniz AU. In-vitro microbial activity of different
electrochemically- activated solutions on enterococcus faecalis. Eur
Oral Res 2019;53(1): 44-50
 Dube K , Jain P. Electrolyzed saline... An alternative to sodium
hypochlorite for root canal irrigation. Clujul Medical Vol. 91, No. 3,
2018: 322-327
 Swanljung et al. Root Canal Irrigants and Medicaments in
Endodontic Malpractice Cases: A Nationwide Longitudinal
Observation. JOE.2018 April;Vol 44(4): 559-564
 Lata S et al. Antibacterial effectiveness of Electrochemically
activated (ECA) Water as a root Canal Irrigant- an in vitro
comparative study. Journal of Clinical and Diagnostic
Research.2016 OCT, Vol-10(10): ZC138-ZC142
 Oculus Innovative Science, Inc. [US/US] International Publication
Number: WO 2006/119300 A2 and PCT/US2006/016856