BIOL 315: Introduction

to Immunology
F2019 Lecture Slides
09/26/2019

Gerald L. Buldak, Ph.D.

Loyola University Chicago
Lymphatic vessels AF
FE
RE Capsule
NT (=Marginal Sinus)
GCs Cortex
Lateral Transverse Sinus Paracortex
Medullary Cords

Subcapsular Sinus
Medulla

Medullary Sinus
Hilar vein
Blood supply
Hilus Hilar artery

Microvessels
• Arterioles EFFERENT
• Capillaries
• Post-capillary venules*
• High Endothelial Venules (HEVs)

N T
E
F ER
AF © 2019, Gerald L. Buldak, Ph.D.
 Collagen

Reticulocytes
(Fibroblast Reticular
Cells, FRCs)

CONDUITS!
© 2019, Gerald L. Buldak, Ph.D.
 Capsule

Capillaries Venous
Penicillar sinus
TRABECULA(E)
arterioles
Central
artery Trabecular
vein

Splenic Artery
Splenic Vein
Trabecular
arteries
Hilus
Spleen pt 1

© 2019, Gerald L. Buldak, Ph.D.

MARGINAL ZONE (MZ)

Red
White pulp
pulp

Spleen pt 2

© 2019, Gerald L. Buldak, Ph.D.

 Spleen pt 3

Marginal zone
(MZ) Venous
Capillary Reticular fibersSplenic
e Sinus cords
Follicle riol Reticulocytes

A rte
a l
r
ent
…C Periarteriolar Lymphoid Sheath
MφS
“PALS”

PALS: TCD4 >> TCD8 > (DC + B)*

Splenic Cords: CONDUITS!!!
Reticular fibers
CONDUITS!!!
Flattened reticular cells
Venous Sinus: All blood cell types (RBCs prevalent)
Follicle: B cells >> everything else SPLENIC MACROPHAGES (MφS)
MZ: “Unconventional” B cells + DCs
© 2019, Gerald L. Buldak, Ph.D.
 Follicle TFH

?
(NO CAPSULE!)

FDC

Cells

Cytokines

Chemokines

B
immature
mature
Bmem
© 2019, Gerald L. Buldak, Ph.D.
 CD34
IL-7R
(α-γC)
VLA-4

Common Lymphoid
Progenitor

(CLP)

B T
© 2019, Gerald L. Buldak, Ph.D.
© 2019, Gerald L. Buldak, Ph.D.
 TCR Co-Receptors

Antigen = EXTRACELLULAR (non-self) peptide

Antigen = CYTOPLASMIC (self) peptide
© 2019, Gerald L. Buldak, Ph.D.
 DN
γδ Tmem

DN1 DN3

T
NK/TCP
DN2 DN4 SP
Teff

HSC CLP

DP1 DP2

B Pro- Pre-“Transitional (T)” immature B

T1 T2

Pre Early Late Large Small Immat. Mature

© 2019, Gerald L. Buldak, Ph.D.
T
DN1 DN3

V-
DP1 DP2

?
DJ
D-J
DN2 DN4

REARRANGEMENT BEGINS

Pro- Pre-

B Pre D-J
Early V-DJ
Late
? © 2019, Gerald L. Buldak, Ph.D.
Large Small
 Notch 1

TCF-1
TFs GATA-3

CD3 RAG-1

© 2019, Gerald L. Buldak, Ph.D.