I.

SYNTHESIS OF poly(beta-amino ester)

1. Synthesis of amine-protected diamine(One amine group is required to graft

polycarboxy betaine and another amine would be required to synthesise
poly(beta-amino ester) by reacting with acrylate.

di-tert-butyl dicarbonate 1,2-Diaminoethane

N-Boc-ethylenediamine
2.Synthesis of amine protected poly(beta-amino ester) by Michael addition reaction

N-Boc-ethylenediamine 1,6-hexanediol diacrylate

N-Boc-PAE
3.Synthesis of Boc Deprotected amino-PAE

amino-PAE
N-Boc-PAE
II.SYNTHESIS of poly(carboxybetaine)

1. Synthesis of CB-tBu monomers (Zwitterionic PCB is only soluble in water / methanol.CB-tBu

monomer has good solubility in anhydrous organic solvents such as acetonitrile and DMF)

+
2-(Dimethylamino)ethyl tert-butyl bromoacetate
methacrylate

CB-tBu monomer
2.Synthesis of NHS-ester initiator (for ATRP) – N-hydroxysuccinimide 2-bromopropionate

N,N'-Dicyclohexylcarbodiimide (DCC)

N-hydroxysuccinimide
N-Hydroxysuccinimide 2-bromopropionic acid
2-bromopropionate
3.Synthesis of NHS-PCB-tBu

N-hydroxysuccinimide
2-bromopropionate

CB-tBu monomer NHS-PCB-tBu

III.CONJUGATION of amino-PAE with NHS-PCB-tBu (Reaction conditions needs to be optimised)

PAE-PCB-tBu