Hepatotoxicity of silver nanoparticles

Ag NPs and Cyp2E1 gene expression on

animal model.

Presented By:
Name:Ammara Usman
Subject: M.Phill Genetics
VU ID: ms170400923
 Supervisory Committee
 Supervisor : Dr Tanveer Hussain
 Member 1(Co-supervisor): Dr Muhammad
Safdar
 Member 2: Dr Masror Ellahi Baber
Introduction
 Hepatotoxicity is a liver damage due to
chemicals. Chemicals that cause liver injury are
called hepatotoxins .
 Cyp2E1 (cytochrome p450 2E1) is a membrane
protein present at high levels in liver. In liver
drugs are deactivated by Cyp2E1 gene. In humans,
Cyp2E1 enzyme is encoded by Cyp2E1 gene.
 Nanotechnology is very popular these days. The
widely used nanoparticles are silver
nanoparticles. They are used for medicinal
purposes .
Introduction
 They are also used in respirators, household
water filters, in cosmetics, detergents and textile.
 Nanoparticles enter in blood stream by
inhalation, skin and gastrointestinal tract. Liver
accumulates the nanoparticles. It is important to
check the toxicity of these NPs in blood stream.
It requires time and cost effective mechanism to
study toxicology.
Objectives

 To induce hepatotoxic animal models

 To evaluate hepatoprotective effects of
Silver NPs
 To check the gene expression of Cyp2E1
 Validation of gene expression via PCR
Questions

 What will be the effects of nanoparticles

on organs like liver and kidney?
 How Cyp2E1 gene expresses itself during
toxicity of these organs?
Material and Methods
Group A
(1g paracetamol
Experimental /body weight)
group
(10 mice)
Group B
(1g paracetamol/body
weight+5ml of Ag
15 albino mice Acclimatization NPs dissolved in 1L
(male + female) for 3 days water)

Control group Group A

(5 mice) (normal feed and
water only)
Sampling (Blood Collection)
Animals will be harboured, on a light/dark cycle
(12/12 h) at room temperature.
Blood will be collected via marginal ear vein of
the rabbits in EDTA/Heparin coated vials. Serum
will be separated from the whole blood by
centrifugation of the samples at 3000 g for
10minutes. The serum will be stored in aseptic
vials at -20 °C. The serum will be kept in the
freezer until blood biochemical estimations are
performed.
Parameters (Biochemical studies)
 Liver tests
1. ALP
2. ALT
 Cholestrol level
 Creatinine
 Urea levels
 Lipid profile
By using standard biochemical kits.
Data Analysis
 Liver analysis:
ALP and ALT tests will be used to test the
difference between controlled and treated groups.
 Gene expression:
Gene expression of Cyp2E1 gene will be analysed
with the help of PCR. We can also use the
quantitative PCR to quantitate the actual levels of
expression.
 Statistical analysis:
All data obtained will be analysed using ±
standard error of deviation via two-way ANOVA.
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