INFLUENCE OF ORGANIC POTTING

MEDIA TO TISSUE-CULTURED
LAKATAN BANANA (Musa sapientum
L.) PLANTLET GROWN
UNDER SCREENHOUSE CONDITION
ROMULO C. SALVADOR Sr., Ph.D
CAA Faculty
Assistant Professor IV
Outline of the Presentation

• Rationale
• Objectives
• Materials and Methods
• Results and Discussion
• Conclusions
Rationale

* Banana (Musa sapientum L.) belonging to the Musaceae family,

is the largest herbaceous flowering plant that grows in all tropical regions.

* This plant comes in a variety of sizes and colors when ripe turns
yellow, purple, and red.
It has a pseudo stem which acts as the main stem but are
actually modified leaves called bracts.

* A pseudo stem can produce a single bunch of bananas.

After fruiting, the pseudo stem dies, but offshoots may develop from
the base of the plant as suckers that are viable for reproduction.
• Micro propagation by shoot culture technique has been develop as means for
mass production of banana
• as an integral component of the ongoing eradication and rehabilitation program in
the Philippines of healthy planting materials (Panabang and Rodrigues, 2009).
• However, lack of capital outlay is one of the major impediments in the small scale
nurseries.
• In-vitro culture is a popular method of vegetative propagating crop plants with
high commercial value.
• Ideally, all plants generated from tissue culture are true to-type i.e. have identical
constitution from that of the mother or original plants.
• Contrary to this, high incidence of off-types is a major concern (Sales and Roca,
2016).
• Banana production is expensive
Rationale from planting to harvesting.
• Farmers unable to grow such crops when it comes to costs due to
plantlet price and quality.
• The banana fruit crop is widely cultivated in tropical areas where
high dosages of chemical fertilizers (Nitrogen, Phosphorus and
Potassium) are commonly applied.
• Commercial cultivation of banana requires a substantial amount of
inorganic fertilizer namely urea (Robinson, 1996).
• Banana plantlet can be grown in nursery without expending much
money.
• This can be done by using good and cheap growing medium that
are readily and locally available.
Objectives

• The study aims to determine the effect of organic potting media

utilized to tissue cultured lakatan banana grown under screen
house condition specifically it aims to:
• Determine which of the organic potting media gives the greatest
growth performance of tissue-cultured lakatan banana,
• evaluate the survival rates of tissue cultured lakatan banana and,
• assess the return on investment in the production of tissue
cultured lakatan banana using different organic potting media
grown under screen house condition.
Materials and Methods

• A study on the influenced of organic potting media utilized

tissue cultured lacatan banana grown under screenhouse
condition was conducted from January to April 2019 at the
Research Experimental Station of Caraga State University,
Ampayon, Butuan City.
• The study was conducted using Randomized Complete Block
Design (RCBD) with six (6) treatments and three (3)
replications.
 Soil Medium Collection and Site Preparation
• The area was cleaned-up and prepared before transplanting of
tissue-cultured banana plantlets.
• Vermicast was collected at Organic Agriculture (OA) production
site of Caraga State University, while the sawdust was collected at
Stockhouse, Barangay Taguibo, Butuan City.
• Coco coir dust was collected at Antongalon, Butuan City.
• Collected soil medium was air dried for one week and sieved.
• One half kilogram of soil sample was filled in the pots and ready
for transplanting.
 TREATMENT 

• T1 Pure Sawdust (SD)

• T2 Pure Coco Coir Dust (CCD)
• T3 Pure Vermicast (VC)
• T4 VC+CCD+SD (1:1:1)
• T5 VC+CCD+SD (1:1:3)
• T6 VC+CCD+SD (1:1:5)
 Results and Discussions

P lant Height M ean (cm)

88

87

86

86.8

85

84.7 84.8
84 84.9
83.9
83.7

83

82
T1 – Pure Sawdust
T1 – Pure Sawdust (SD)
T2 –T2 Pure Coco Coir
– Pure Coco Coir Dust (CCD) T3 –T3Pure Vermicast
– Pure Vermicast (VC) T4 – T4
VC + +CCD
– VC + SD
CCD + SD (1:1:1)
(1:1:1) T5 –T5VC
– VC+ CCD
+ CCD +SD
+ SD (1:1:3)
(1:1:3) T6 – T5
VC ++CCD
– VC +SD
CCD + SD (1:1:5)
(1:1:5)

TREATMENTS

Figure 1. Plant Height of Tissue Cultured Lakatan Banana

Plantlet as Influence by Organic Potting Media
 14

12

13.0a
10
Stem Diameter Mean (cm)

4.8b 5.1b
2
4.4b 4.2b
2.6c

0
T1 –T1Pure Sawdust
– Pure Sawdust (SD) T2
T2 –– Pure
Pure Coco
Coco Coir Coir
Dust (CCD)
T3T3–– Pure
Pure Vermicast (VC)
Vermicast T4 – VC + CCD + SD (1:1:1)
T4 – VC + CCD + SD (1:1:1)
T5 – VC + CCD + SD (1:1:3)
T5 – VC + CCD +SD (1:1:3)
T5 – VC + CCD + SD (1:1:5)
T6 – VC + CCD +SD (1:1:5)
TREATMENTS

Figure 2. Stem Diameter of Tissue Cultured Lakatan Banana Plantlet

as Influence by Organic Potting Media
 7

6.5a
Number of Leaves Mean

3.6b
2
3.4c
2.8c 3.0c

1 1.9d

0
T1 – Pure Sawdust (SD) T2 – Pure Coco Coir Dust (CCD) T3 – Pure Vermicast (VC) T4 – VC + CCD + SD (1:1:1) T5 – VC + CCD + SD (1:1:3) T5 – VC + CCD + SD (1:1:5)
T1 – Pure Sawdust T2 – Pure Coco Coir T3 – Pure Vermicast T4 – VC + CCD + SD (1:1:1)
TREATMENTS T5 – VC + CCD +SD (1:1:3) T6 – VC + CCD +SD (1:1:5)

Figure 3. Number of Leaves of Tissue Cultured Lakatan Banana Plantlet

as Influence by Organic Potting Media
 90

80

70

83.3a
60 70.0c 76.7b
76.7b
50
60.0d
Survival rate %

40

30 46.7d

20

10

0
T1 – Pure Sawdust (SD) T2 – Pure Coco Coir Dust (CCD) T3 – Pure Vermicast (VC) T4 – VC + CCD + SD (1:1:1) T5 – VC + CCD + SD (1:1:3) T5 – VC + CCD + SD (1:1:5)
T1 – Pure Sawdust T2 – Pure Coco Coir T3 – Pure Vermicast T4 – VC + CCD + SD (1:1:1) T5 – VC + CCD +SD (1:1:3) T6 – VC + CCD +SD (1:1:5)
TREATMENTS

Figure 4. Survival Rate of Tissue Cultured Lakatan Banana Plantlet as

Influence by Organic Potting Media
   Marketable plantlets Gross income Total expenses Net return (Php) ROI
TREATMENTS (pcs) (Php) (Php) (%)

Saw Dust
16 480 262.86 217.14 82.61

Coco Coir Dust

23 690 276.19 413.81 149.83

Vermicast
18 540 296.19 243.81 82.32

VC+CCD+SD (1:1:1)
25 750 309.52 440.48 142.31

VC+CCD+SD (1:1:3)
21 630 309.52 320.48 103.54

VC+CCD+SD (1:1:5)
23 690 309.52 380.48 122.93

TOTAL
146 4380 2003.80 2376.20 833.54

Mean
20.86 625.71 286.26 339.46 119.08

Figure 5. Return on Investment of Tissue Cultured Lakatan Banana Plantlet as Influence by Organic Potting Media
Conclusions

• As observed from the different organic potting media utilized in

the production of tissue cultured lakatan banana plantlet under
screenhouse condition it was concluded that
• T3 – Pure vermicast enhanced the greatest growth performance
of tissue cultured lacatan banana grown under screenhouse
condition both in plant height, stem diameter and number of
leaves.
* On the survival rate T4 – VC + CCD + SD (1:1:1) enhanced
the highest survival rate of tissue cultured lakatan banana
grown under screen house condition of 83.3%.
* While Treatment 2 - Coco coir dust enhanced the greatest
return on investment with a rate of 152.44%.
Thank You!