Short Manual for NEO-1200 Operation

Short Manual for NEO-1200 Operation

1. INSTALLATION ....................................................................................................................................................... 2

1.1. INSTRUMENT CONNECTION ......................................................................................................................................... 2

1.2. DRIVER INSTALLATION ................................................................................................................................................ 2
1.3. SOFTWARE INSTALLATION ............................................................................................................................................ 3
1.4. COM PORT SETTING .................................................................................................................................................. 3
1.5. INITIALIZATION .......................................................................................................................................................... 4

2. PARAMETERS SETTING .......................................................................................................................................... 5

3. REAGENT POSITION SETTING ................................................................................................................................ 6

4. CALIBRATION ........................................................................................................................................................ 7

5. QC......................................................................................................................................................................... 9

5.1. QC INFORMATION SETTING ......................................................................................................................................... 9

5.2. QC APPLICATION ..................................................................................................................................................... 10
5.3. QC VIEW ............................................................................................................................................................... 10

6. PATIENT SAMPLE TEST ......................................................................................................................................... 12

6.1. ROUTINE SAMPLE TEST ............................................................................................................................................. 12

6.2. BATCH TEST ............................................................................................................................................................ 12

7. MAINTENANCE AND TROUBLE-SHOOTING .......................................................................................................... 14

7.1. DAILY MAINTENANCE ............................................................................................................................................... 14

7.2. CUVETTE CHANGE ................................................................................................................................................... 14
7.3. LAMP AD ADJUSTING ............................................................................................................................................... 15
7.4. SOUND ALARM ....................................................................................................................................................... 16
7.5. ABNORMAL RESULTS ................................................................................................................................................ 17
Short Manual for NEO-1200 Operation

1. Installation

1.1. Instrument connection

DI Water Bottle (10L) Waste Bottle (10L)

Connect the liquid pipeline as above picture.

1) The left green pipeline is for inlet of detergent solution, the other two green pipelines are for inlet of water
for dispensing and washing. The right yellow pipelines are for outlet of dispensing and system washing.
2) When connect the pipeline, the connector should be connected with the water filter. Make sure this end sink
in the bottom of bottle. The washing function on the instrument can help clear air bubble in the pipeline.
3) The bottle should be full with DI water. A water bucket with a volume 10 liter is recommended. Manufacturer
may not provide such bucket along with the analyzer.

1.2. Driver installation

The software mentioned in this manual with version 5.1.1 is applicable to NEO1200 in 2019 and later. Any
NEO1200 prior to this date may require old version software. Please contact your supplier if needed.
1) Connect the instrument (USB port) and PC (USB port) with the USB port communication cable.
2) Run the USB driver provided by the manufacturer in the file CP210x_Windows_Drivers.zip
3) Enter Device Manager of computer to find the Ports (COM&LPT), record the COM No. (COM3 for example)
 Short Manual for NEO-1200 Operation

1.3. Software installation

The PC hard-disk should have the D disk. If it is not available, IT engineer can help do hard-disk partition and keep
one disk name as “D”.
Find and install the software Automatic Biochemistry Analyzer V5.1.1.msi provided by the supplier.
The database shall be located at disk D: Nova.
If there pop up any tip about the .NET error, please install the program DotNetFX40.exe

Reminder of lower version NET Framework installation NET

1.4. COM port setting

1) Power on equipment and login software (password “admin”). British punctuation marks are required all the
time for any input of characters and numbers on this software.
2) In the initial software login, it may popup message “serial open failed”. We need to enter the software and set
the correct COM port.
shown in the Device Manager (COM 3), 【 Save 】 and login again the software.

After login software, if the instrument communication with PC is in good condition, the instrument will do
mechanical initialization. The red LED shall emit light through the reaction cuvette No. 15. The mechanical
position shall be shown as below:
Short Manual for NEO-1200 Operation

Reagent Position 1

Probe move and turn back to pool

1.5. Initialization

Enter the interface Debug with password 123456 to check and initialize all pipelines.
1) Make sure the water bottle full of DI water.

times.
Short Manual for NEO-1200 Operation

2. Parameters Setting
The parameters marked by red box in the following Item Setting section are very important. Please refer to your
reagent kit insert and find out the concerned information.

1) Sample and reagent volume setting

The sample volume on this instrument should be 5uL to 35uL, stepping at 1uL. Minimum 5uL is suggested.
The reagent 1 volume should be in the range of 150uL to 400uL, stepping at 1uL.
The reagent 2 volume should be in the range of 20uL to 300uL, stepping at 1uL.
When match the volume of sample and R1/R2, make sure the total volume added to reaction cuvette is within
the range 160uL and 400uL. Recommended total volume is 300uL.
2) Test point setting
 The test cycling time on this model is 30 seconds. One cycle is one point. Total points is 31(0, 1, 2, 3, 4…30).
The reagent mixing time and monitoring time can be converted into points as each minute is 2 points.
 For the End method with mono reagent, the point refers to the end of the reaction. The pre-blank is by
default 0 and 0 respectively. The test points (start point and end point respectively) can be set as 18 and 20.
 For the End method with dual reagent, the start point refers to the R1 and sample mixture, so we set 18
points plus the mixing time as start point. The end point refers to the end of the reaction, so we set 2 more
points plus the start point as the end point. The pre-blank can be set as 12 and 13 respectively.
 For the Kinetic or Fixed-time method, the start and end point refers to the interval between the points when
the reaction becomes stabilized. We set 18 points plus the mixing time as start point, and set the continuous
monitoring time (minimum 3minutes) plus the start point as the end point.
 Based on the above calculation, operators can check the actual reaction curve, find a stable time period,
turn back to the parameter setting section and adjust the test point accordingly.
3) Other settings

The Places is for the decimal places. The absorbance, Male and female range, health item, linearity, correction
factory, reaction direction and dynamic can be set by default if not required in use.
 Short Manual for NEO-1200 Operation

3. Reagent Position Setting

Whenever we want to run new test, we need to first of all register and set reagent parameters in the interface
Item parameters, then we can set new positions for reagents in the interface Reagent.
When load reagents to reagent bottles, for mono reagent, we put R1 to bigger bottle. For dual reagent, we put R1
to bigger bottle and put R2 to smaller bottle.

1) Reagent Disk Number: it is the virtual reagent disk number. Number can be set as 1 or 2. Each disk has 16
positions for reagent. If use no more than 16 reagents kits, we only set disk 1. If use more than 16 reagent at
the same time, the disk No. 2 is needed and set reagent position as 2.
2) Test Name: name of reagent for test, name it in short.
3) Type: set 1 for Reagent 1 and set 2 for Reagent 2.
4) Position No.: the position on the reagent tray where we load the reagent kit. If it’s dual reagent we should
set same position for Reagent 1 and Reagent 2.
5) Total Capacity: set by default, Reagent 1 at 28ml, Reagent 2 at 7ml.
6) Residual: residual of reagent in the kit by default.
7) Measurable sample quantity: Set by default.
8) Margin detection and full detection allow the detection of reagent residual in the kit on board.

Click 【 Add 】 to create new location for new reagent, input required information and click 【 Save 】 , it will be added
to the reagent tray list. For dual reagent, the R1 and R2 should be set in same position respectively. For example,
UA is dual reagent. We set R1 in position 3 and set R2 in Position 3 again.
Notice: when logout the software, the instrument probe will move and stop at reagent position, please take
away the reagent tray before logout software.
Short Manual for NEO-1200 Operation

4. Calibration
Reagent parameters and Reagents positions must be set before running its calibration.
Some laboratories may need to do calibration and quality control before running tests with samples.

select the calibration item and 【 Confirm 】 .

2) Select Calibration method, input Batch No., Calibrators Num, Standard Num and Number of repeated tests.
Click 【 Add 】 to add it to the list. Input calibrator Concentration and Cup position. Click 【 Save 】 , the calibrator
will be automatically listed in “Untested” list for calibration.

the following picture.

 Short Manual for NEO-1200 Operation
Calibration method
 Linear: only one calibrator, fitted linear from zero and calibration point.
 Two point Linear: Two calibrators, fitted linear from two calibration points.
Notice: This is the most common method for calibration. General biochemistry has only 1 calibrator, we put
distilled water for 0 point, and put calibrator for second point.

4) After finishing calibration, enter Calibration History section. Select one calibration on the Calibration history
table and 【 Set Current 】 , it will be validated and added to Scaling parameter table.

5) After calibration validation, we can run sample test with this reagent. The calibration reaction curve can be
viewed by double click on one calibration record.
6) Operators can run a QC as sample for three times and make the mean value, and compare this with the
calibration result. This result can be used to determine if the calibration is good.
 Short Manual for NEO-1200 Operation

First calibrator reaction curve Second calibrator reaction curve

5. QC

5.1. QC information setting

Some laboratories may need to run Calibration and Quality Control before running specific tests.
At QC Set section, we can set QC information
1) Name: the control name
2) Batch: the control lot number
3) Position: which sample tray position will put
4) Validity: the expiration date
5) Type: low, middle or high level
Click 【 Add 】 , input one QC info and 【 Save 】 , this QC will be listed.
In the middle there is “Item info” which is a list of reagent calibration validated for QC. It is available only after
finishing calibration and validation.
1) Select a QC, select an item and double click this item, the Item will appear at right side QC item list.
Short Manual for NEO-1200 Operation

5.2. QC application

1) Select a QC Product.
3) The “Test Items” is QC waiting list.

5.3. QC view

In QC view section, we can inquire, view and print QC result.

Short Manual for NEO-1200 Operation
 Short Manual for NEO-1200 Operation

6. Patient sample test

6.1. Routine sample test

2) Input necessary information for the sample.

3) Choose the tests for the sample in the middle of the available test board. Multiple tests and Portfolio can be
chosen.

5) Choose one or more tests on the list on the right side.

6.2. Batch test

Click 【 Batch Add 】 to pop up the setting window.

1) In the left side Item list we can select and double click to add it to middle list Select Item.
2) Sample Number: input the sample number for batch test.
3) Start number: define a position to start testing.
4) Fixed: Select fixed position if the sample is in same position. In this case the Sample Number is the repeating
times.
Short Manual for NEO-1200 Operation
6) Turn back to the Sample interface and continue test.

7) When we start testing, do not switch to any other window until the ongoing tests are all finished. Otherwise
there might be unknown error to the system.
Short Manual for NEO-1200 Operation

7. Maintenance and Trouble-shooting

7.1. Daily maintenance

In the morning and before shutting down instrument, we use alkaline solution to clean the reagent/sample probe.
Enter the interface Debug with password “123456” and 【 Clean All 】 for cuvette maintenance.
When to clean cuvette?
1) In the morning before test.
2) Before/after calibration.
3) When there is sudden power off during test.
4) Test result is not good.
5) Replace new cuvette

7.2. Cuvette change

Generally in 3 to 6 months the reaction cuvettes should be replaced with new ones. A backup cuvette disk as
picture 7-1 can be found in the package by supplier.
Operation protocol:
1) Remove the washing station Pic 7-2
2) Remove the reaction disk fixed screw Pic 7-3
3) Remove the cuvette disk fixed screws Pic 7-4
4) Replace new cuvette and note the position 1 points to the mark position Pic 7-5
5) Fixed the reaction disk screw. Note there is an O-ring.
6) Fixed the washing station screw.

Note: after fixing the washing station, maybe the position is different. If position is not good, please loose a little
the washing station fixed screw and manually adjust its position.
Short Manual for NEO-1200 Operation

Pic 7-1 Pic 7-2 Pic 7-3

Pic 7-4 Pic 7-5 Pic 7-6

7.3. Lamp AD adjusting

When the test result is not good or after replacing new cuvette we should adjust the AD value of lamp.
1) Enter the interface Debug with password novas6807.

5) The AD value should be around 3300-3700.

adjust the gain of LED.

8) This protocol applies to different wavelength.

Short Manual for NEO-1200 Operation

Notice:
1) Before testing LED AD, please wash cuvette to make sure the cuvette is clean. If cuvettes are used for more
than 6 mouths please replace with new one.
2) Before testing AD, please dispense water to all cuvette. If there is no water in the cuvette, the AD value will
not be stable.
3) After finishing AD test, please drain all water from cuvette.

7.4. Sound Alarm

When there is sound alarm, the analyzer is in trouble.

5 times sound alarm: this means the pipetting probe is not in expected position and should be corrected.

4 times sound alarm: this may be the problem of the optocoupler position or AD value. We need to eliminate the
causes.

instrument will move respectively its pipetting arm, washing station, reaction tray, reagent tray and
dispensing pump. The results will be displayed at the bottom of the table, the record marked in red indicates
possible problem and that spare shall be checked, repaired or replaced.
 For AD value problem, the cause can be one or more wavelength LEDs giving AD lower than 2000, the
problem of reaction cuvettes or the problem of electric board. Then the AD shall be adjusted, the cuvettes
shall be checked and replaced, and the electric board shall be replaced accordingly.
Short Manual for NEO-1200 Operation

7.5. Abnormal Results

When there is abnormal result, we need to eliminate the possible reasons from the following factors.
1) Check if the reaction curve is in expected shape.
2) Check if the reagent volume is enough or overloaded, position on the tray is correct, batch No. is correct and
valid; if there is re-calibration for new reagent; if the sample volume is enough, position on the tray is correct
and pre-treated; if there is insufficient dispensing or over-dispensing of DI water.
3) Check if the AD value is normal and make adjustment and recalibrate.
4) Check if the reaction cuvette is in good condition.
5) Check if the instrument and power supply is grounding well.

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For more information or technical assistance, please contact
Rain at rain.zeng@zecenbio.com
Leo at leo.wu@zechengbio.com
Arvin at arvin.li@zecenbio.com