• DnaA- Positive modulator of DNA Replication

• SeqA –Negative modulator of DNA replication

-DNA Methylation and Attachment to the Membrane
Control Initiation of Replication

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• Dna A –a positive modulator of DNA
replication (discussed in previous lecture)

SeqA –Negative modulator of DNA

replication

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SeqA will bind and sequester the The fully methylated DNA
hemimethylated daughter origins to the does not bind to SeqA, no
membrane, preventing more DnaA binding. membrane association,
As a result of its membrane association, DnaA can bind and
methylation of oriC is delayed about 13 reinitiate replication from
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minutes after initiation. OriC
• Seq A protein is a negative regulator of DNA initiation.
• SeqA selectively binds to origin (oriC) and inhibits
reinitiation of replication from OriC after the replication has
proceeded.
• The oriC contains 11 copies of GATC palindromic
sequences. When the OriC is fired, the daughter strand are
hemi-methylated.
 Over most of the chromosome, full methylation is restored
within a minute or two of replication . This brief period allows
for use of hemi-methylation as a guide for the mismatch
repair system . However, the origin of replication is slow to
regain full methylation; it takes 10 to 15 minutes.

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• SeqA will bind and sequester the
hemimethylated daughter origins to the
membrane, preventing more DnaA binding. As a
result of its membrane association, methylation
of oriC is delayed about 13 minutes after
initiation.
The fully methylated DNA does not bind to
SeqA, no membrane association, DnaA can bind
and reinitiate replication from OriC

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