SUBSTRATE

CONCENTRATIO
N
Created by: Neelam, Tamu and Chiedza
THE EFFECT ON ENZYME-
CATALYZED REACTION.
 The higher the substrate concentration, the faster the reaction
 more substrate molecules means a collision between substrate and enzyme is
more likely and so more active site will be used.
 This is only true up until a ‘saturation’ point. After that, there are so many
substrate molecules that the enzymes have about as much as they can cope with
(all the active sites are full), and adding more makes no difference.
 Substrate concentration decreases with time during a reaction (unless more
substrate is added to the reaction mixture), so if no other variables are changed,
the rate of reaction will decrease over time too. This makes the initial rate of
reaction (the reaction rate at the start) the highest rate of reaction.
SUMMARY
Increasing substrate concentration also increases the
rate of reaction to a certain point. Once all of the
enzymes have bound, any substrate increase will have
no effect on the rate of reaction, as the available
enzymes will be saturated and working at their
maximum rate.