Cobalt-Based MOF as

Antibacterial Activity

Presentad By: Nadeem Abbas (f2018140037)

 Bioinorganic Chemistry
 Bioinorganic chemistry is a field that examines the role
of metals in biology.
 Bioinorganic chemistry includes the study of both natural
phenomena such as the behavior of metalloproteins as well as
artificially introduced metals, including those that are non-
essential, in medicine and toxicology. Many biological
processes such as respiration depend upon molecules that fall
within the of inorganic chemistry.
 MOFs
• Metal–organic frameworks (MOFs) are a class of compounds
consisting of metal ions or clusters coordinated
to organic ligands to form one-, two-, or three-dimensional
structures. They are a subclass of coordination polymers, with the
special feature that they are often porous.
Paper
 Cobalt-Base MOFs
 Co-based metal-organic framework have drawn increasing
attention as electrochemical applications due to their facile
preparation methods, adjustable sizes, tunable structures and
high specific surface areas. Co-based MOFs with highly
antibacterial activity and evaluated for use as a bactericidal
material against the gram-negative bacteria Pseudomonas
putida and Escherichia coli. Co-based MOFs exhibit a high
antimicrobial activity towards photosynthetic organisms such
as cyanobacteria. Thus, Co-based MOFs show a great
potential against pathogenic bacteria.
 Cobalt-Base MOFs
• Two Co-based MOFs, namely,
 {Co(Hin)2}n·DMF
 {Co2 (Hin)4}n
• MOF architectures are solvothermally synthesize from the
reaction of isonicotinic acid and cobalt chloride under
different pH conditions. The antibacterial activity is evaluate
by the transparent ring method and well diffusion method. The
gram-negative bacteria E. coli and gram-positive bacteria S.
aureus.
 Synthesis of MOFs
1
 CoCl2·6H2O (0.0238 g, 0.1 mmol) and Hin (0.018 g, 0.15
mmol) in the solvent of DMF (6 mL) were placed in a Teflon-
lined stainless steel autoclave (20 mL). The pH condition 5.3–
5.6 with 0.5 mol· L-1 hydrochloric acid aqueous solution.
After the adjustment of the pH condition, the mixture sealed
and heated at 120 °C for 72 h and then cooled to room
temperature at a rate of 2 °C·h−1 . Purple crystals of complex
1 obtained by filtration, washing with DMF and acetone.
 Synthesis of MOFs
2
 The synthetic method for the preparation of complex 2 is
almost identical to that of complex 1, except pH condition
approximately 4.8–5.0 with 0.5 mol·L-1 hydrochloric acid
aqueous solution. Pink crystals of complex 2 obtained by
filtration, washing with DMF and acetone.
 Structure description of
{Co (Hin)2 }n·DMF (1)
 The central Co ions are six-coordinated with four
oxygen atoms and two nitrogen atoms from the four
independent Hin ligands forming the coordination
geometry. The bond angles around the central Co ions
are in the range of 29.8 Å to 165.6 Å.
 Empirical formula C12H8CoN2O4·C3H7NO
 Structure description of
{Co2(Hin)4}n (2)
 Two central Co ions and four Hin ligands. Each Co
ion is six-coordinated by four oxygen atoms and two
nitrogen atoms from six independent Hin ligands,
showing an octahedron coordination.
 Empirical Formula: C24H16Co2N4O8
 Characterization Techniques
Both compounds are characterize by following techniques.

 FT-IR
 TG
 PXRD
 IR
 TGA curves of complexes
 Complexes similar
pyrolysis trends.

 Decomposition occur at
approximately 200–400 °C,
leading to the predominant
weight loss.

 Before 200 °C, the weight

loss corresponds to the
remove of water from the
surfaces of MOF materials.
 Antibacterial Activities
 The antibacterial activity of both complex against E.
coli and S. aureus is measured by the transparent ring
method.
 The antibacterial activity of both complexes
the MIC values against E. coli and S. aureus
are also measured by the well diffusion
method.
Antibacterial Activities
 Antibacterial Activities
Complex 1 Complex 2 Penicillin Hin CoCl2 DMSO

Concentratio 100 100 100 100 100 --

n(μg·mL−1 )

Staphylococc 14.23 ± 0.11 13.56 ± 0.17 16.89 ± ~10 0 0

us aureus 0.13

Escherichia 13.02 ± 0.22 13.25 ± 0.1 16.15 ± ~9.9 0 0

coli 0.12

 The diameters of transparent circle of tested samples and positive

control (mm)
 Antibacterial Activities
1 2 Penicillin Hin

Escherichia coli 12.5 12.5 3.1 75

Staphylococcus 6.25 12.5 6.5 50
aureus

 MIC value of 6.25 μg/mL against S. aureus can be obtained for

complex 1, which demonstrates that complex 1 has an antibacterial
activity against S. aureus that is similar to that of the positive
control.
 Complex 2 has MIC value of 12.5 μg/ mL against S. aureus and E.
coli
 Conclusion
 We demonstrates that it is possible to use cobalt-based metal
organic frameworks as antibacterial materials. The materials exhibit
remarkable antibacterial activity and durability, due to the control
of the release of cobalt ions in biocidal solutions. The antibacterial
activities of both complex are investigated by determining their
minimal inhibitory concentration values against gram-positive
bacteria and gram-negative bacteria which are bacteria commonly
encountered in our daily environments. The results shows that both
complexes are more sensitive to S.aureus than to E.coli and their
preparation with simple, relatively cheap and easily accessible
commercial ligands, leading to a more affordable possible future
application as antimicrobial materials.
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