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Chromatography
Chromatography is a of separation in which
method
the components to be separated are distributed
betweenonetwo
phases, of these is called a stationary phase and the other is
a mobile phase which moves on stationary phase in a definite
direction. The component of the mixture redistribute
themselves between two phases by a process which may be
adsorption, partition, ion exchange or size exclusion.
The stationary phase can be solid or a liquid and the mobile
phase can be liquid, gas or a supercritical fluid.
Illustration of Chromatography
Separation
Mobile Phase
Mixture Components
Examples of Chromatography
On the basis of interaction of solute On the basis of chromatographic bed On the basis of physical state
to the stationary phase shape of mobile phase
Partition Chromatography
Liquid-liquid Gas-liquid
Chromatograph Chromatograph
y y
Ion Exchange Chromatography
Definition:
Ion Exchange Chromatography (Ion
Chromatography) is a process that allows the separation of
ions and polar molecules based on their affinity to the ion
exchanger. It can be used for almost any kind of charged
molecules including large protein, small nucleotide and amino
acids. The solution to be injected is called Sample and
individually separated components are called analytes. It is
often used in protein purification, water analysis, and quality
control.
Principle:
Ion Exchange Chromatography is based on the relative
retention of the ions during their progress through an ion
exchange column which has functional group of opposite
charge attached to its surface. The stronger the charge on the
ion, the greater is the retention time in the column.
Liquid-Liquid Liquid-Solid
Chromatography Chromatography
Stationary
Liquid Solid
Phase
s
_CHromatography.jpg
http://xray.bmc.uu.se/Courses/MPC/students_files/ION_EXCHANGE_CHROMATOGRAPH
Y.pptx
http://www.thefreedictionary.com/chromatography
http://www.britannica.com/EBchecked/topic/115917/chromatography/80518/Liquid-
chromatography
http://www.ltt.com.au/simulab/5/Laboratory/StudyNotes/snClassifChromatoMeth.ht
m
http://www.rpi.edu/dept/chem-eng/Biotech-Environ/CHROMO/be_types.htm
http://en.wikipedia.org/wiki/Paper_chromatography
http://en.wikipedia.org/wiki/Aqueous_normal-phase_chromatography
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