Model: PIS

Rabia Haider
Mphil (Pharmacology)
2nd Semester

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Contents
Introduction
Material & Methods
Behavioral Testing
Results
Conclusion

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Introduction
 Postoperative pain is highly debilitating and hinders recovery.
 Opioids are the main pain medication used for acute postoperative pain.
 Due to opioid addiction and overdose epidemic ,non-opioid pain therapeutics
are needed.
 In order to develop novel, non-opioid therapies for the treatment of
postoperative pain and identify the mechanisms underlying this pain, mice
models of incisional pain have been established.
 The protocol herein describes in detail how to create a mouse model of
postoperative pain that is adapted from established protocols.
 This model of postoperative pain is frequently-used, highly reproducible, and
results in peripheral and central nervous system alterations.
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Material & Methods
• Materials • Equipment

Adult male 20- to 30-g Small animal surgery board 

Stainless steel sterile surgical Forceps
blade
1,000 ml beaker
Sterile nylon surgical sutures
Surgical tape
Extra fine point permanent marker
Cotton swab
Sterile gloves
Tramadol (50mg/Kg)
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Plantar Incision Surgery

 Mice were anesthetized with Tramadol (50mg/kg)

 A 5-mm longitudinal incision was made with sterile surgical blade
through the skin and fascia of the plantar foot.
 The incision was started 2 mm from the proximal edge of the heel and
extended toward the toes.
 The underlying muscle was elevated with a curved forceps, leaving
the muscle origin and insertion intact.
 The skin was apposed with suture
 The suture was removed at the end of postoperative day 1.

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Behavioral testing
Following tests were performed
1) weight Assessment Test
2) Paw Edema test
3) Von-Frey
4) Thermal Hyperalgesia
5) Inverted Screen test
6) Muscle Strength test

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Results
 Results are attached as Separate sheets

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Conclusion
• Plantar incision in mice enhances responsiveness to mechanical and
thermal stimuli that are indicative of primary mechanical and thermal
hyperalgesia.
• Primary mechanical and heat hyperalgesia after incision in mice is
blocked by Tramadol
• Our observations indicate that the mouse model of incision-induced
pain could be a reliable, useful tool to investigate the mechanisms of
postoperative pain