BIOCHEMICAL REACTOR

ASSIGNMENT CH-6
CITRA DEWI RAKHMANIA

02211850012005
Problem 6.3
 ASUME μ max 0.1

a. Kinetics Parameter Ks
KI
0.1
0.2
Cs μ exp μ non lin eror Cs μ exp μ non lin eror
0.5 0.2 0.027027 0.029920 30 0.5 0.000662 0.249338
0.7 0.25 0.021538 0.052195 22 0.6 0.000901 0.358920
1.1 0.35 0.015172 0.112110 15 0.7 0.001316 0.488160
1.6 0.5 0.011034 0.239087 SUM 2.648642
3.3 0.7 0.005704 0.482046
10 0.8 0.001960 0.636867

 
• Assume the value of μ max, Ks and
• Optimize the SSE using solver in Ms. Excel
KI
• Using Cs data form experiment,
calculate𝐹μ
 𝜇 =
𝑒𝑥𝑝
𝑉

• Calculate the square of error for

every Cs Set Objective : SSE to min
By Changing Variable Cells : μ max, Ks and KI
• Sum all of the square of error for Click Solve
every Cs
NONLINEAR

Cs μ exp μ non lin eror Solver μ max 1.739442

0.5 0.2 0.192514 0.000056
0.7 0.25 0.257063 0.000050 Result Ks 3.997998
1.1 0.35 0.368429 0.000340 KI 12.68577
1.6 0.5 0.479863 0.000405
3.3 0.7 0.703758 0.000014
10 0.8 0.794959 0.000025
30 0.5 0.497248 0.000008
22 0.6 0.596523 0.000012
1.7394
15 0.7 0.710275 0.000106  𝜇=
3.9979 𝐶𝑠
SUM 0.001016 ( 1+
𝐶𝑠
+
12.6857 )
NONLINEAR
 𝐹𝑜𝑟 𝐶 𝑠𝑖 =30 𝑔
𝐿
Cs μ exp μ non lin eror Solver μ max 1.752664
0.5 0.2 0.192514 0.000065
0.7 0.25 0.257063 0.000042 Result Ks 4.045541
1.1 0.35 0.368429 0.000319 KI 12.69345
1.6 0.5 0.479863 0.000417 1.7 526
3.3 0.7 0.703758 0.000025  𝜇=
4.0455 𝐶𝑠
10 0.8 0.794959
SUM
0.000000
0.000868
( 1+
𝐶𝑠
+
12.6 934 )

 𝐹𝑜𝑟 𝐶 𝑠𝑖 =6 0 𝑔
𝐿
Cs μ exp μ non lin eror Solver μ max 2.822627
30 0.5 0.500004 0.000000
22 0.6 0.599961 0.000000 Result Ks 14.19329
15 0.7 0.700010 0.000000 KI 7.190633
SUM 0.000000  𝜇= 2 .8226
14.1933 𝐶𝑠
( 1+
𝐶𝑠
+
7.1906 )
 b. Steady State Cell Concentration
•  

 
 
 c. Inhibition Process
• Based on these equation, we know
that Inhibition Process is an
 𝜇= 𝐹 unpredictable because it is a function
𝑉 of many variables, such as Flowrate,
Substrate inlet concentration, yield
growth, Cell concentration, and
𝐾 𝑠 𝐶𝑠 𝜇𝑚𝑎𝑥 𝑉 Monod Kinetics Coefficient.
 
1+ + = • Inhibition Process is an reversible
𝐶𝑠 𝐾 𝐼 𝐹
process,
 𝐶 𝑠 𝜇 𝑚𝑎𝑥 𝑉 𝐾𝑠
• With the increasing of F, KI
= −1 −
𝐾𝐼 𝐹 𝐶𝑠 will increase, it means the
𝐶𝑠 inhibition process will get
 𝐾 𝐼 = faster to reach the
𝜇𝑚𝑎𝑥 𝑉 𝐾𝑠
[ 𝐹
−1 −
𝐶𝑠 ] equilibrium state
• But, with the increasing of Cs,
  𝑪𝑿 KI is a little difficult to predict,
𝑲 𝑰=
( 𝑪 𝑺𝒊 −
𝒀 𝑿/𝑺 ) because the kinetics rate is in
𝝁𝒎𝒂𝒙 𝑽 𝑲𝒔 quadratic function of Cs

[ 𝑭
− 𝟏−

( 𝑪 𝑺𝒊 −
𝑪𝑿
𝒀 𝑿/𝑺 ) ]